Phorbol myristate acetate and Bryostatin 1 rescue IFN-gamma inducibility of MHC class II molecules in LS1034 colorectal carcinoma cell line.


BACKGROUND:The expression of major histocompatibility complex class II (MHCII) antigens in both mouse and human tumors is rare, and these antigens are not easily inducible by IFN-gamma (IFNg). Since MHCII may play an important role in the development of host antitumor immune response, we explored the possibility of restoring MHCII inducibility in several IFNg-resistant tumor cell lines using protein kinase C (PKC) agonists phorbol myristate acetate (PMA) or Bryostatin. RESULTS:Tumor cells were co-cultured with various concentrations of PMA and IFNg for 48 hr. The expression of MHCII antigens and receptors IFNgR1 and IFNgR2 was determined by flow cytometry. We showed that the presence of as little as 0.1 ng/ml of PMA in tissue culture restored the ability of weakly inducible LS1034 colon carcinoma cells to express MHCII in response to IFNg (100 - 10,000 IU/ml) in a dose-dependent manner. Likewise, Bryostatin 1, as low as 10 ng/ml produced a 5-6 fold upregulation of MHCII. The effect of PMA was not observed in two other poorly responding cell lines, MSTO-211H mesothelioma and HepG2 hepatocellular carcinoma, and was abrogated by relatively high concentrations of PKC inhibitors staurosporine (100 nM) and GF 109203X (1,000 nM). Both surface and intracellular staining of all cell lines with antibodies against IFNgR1 and IFNgR2 failed to detect any increase in IFNg receptor expression following incubation with PMA. CONCLUSION:In this study we showed that IFNg-inducibility of MHCII antigens in weakly inducible LS1034 colorectal carcinoma cell line can be rescued by concomitant incubation with PKC agonists. Bryostatin 1 may be considered for further investigation of IFNg-dependent MHCII induction in resistant tumors in vivo.


Cancer Cell Int


Kudinov Y,Wiseman CL,Kharazi AI





Has Abstract


2003-03-25 00:00:00








  • Human urothelial carcinoma cell response to Sunitinib malate therapy in vitro.

    abstract:OBJECTIVES:Bladder transitional cell carcinoma (TCC) is one of the most common solid malignancies in China. This study examined the antitumor effect and underlying mechanism of action of sunitinib malate in human bladder TCC in vitro. METHODS:Bladder TCC cell lines 5637 and BIU87 were maintained in 1640 medium and T24...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Wen J,Li HZ,Ji ZG,Jin J

    更新日期:2015-02-28 00:00:00

  • Investigation of the apoptotic way induced by digallic acid in human lymphoblastoid TK6 cells.

    abstract:BACKGROUND:The digallic acid (DGA) purified from Pistacia lentiscus. L fruits was investigated for its antiproliferative and apoptotic activities on human lymphoblastoid TK6 cells. METHODS:We attempt to characterize the apoptotic pathway activated by DGA. Apoptosis was detected by DNA fragmentation, PARP cleavage and ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Bhouri W,Boubaker J,Skandrani I,Ghedira K,Chekir Ghedira L

    更新日期:2012-06-11 00:00:00

  • Downregulation of long non-coding RNA MAFG-AS1 represses tumorigenesis of colorectal cancer cells through the microRNA-149-3p-dependent inhibition of HOXB8.

    abstract:Background:Colorectal cancer (CRC) is considered as the second common death-induced cancer. More recently, association of long non-coding RNAs (lncRNAs) with CRC has been extensively investigated. Therefore, the present study was performed to determine whether lncRNA MAF BZIP Transcription Factor G Antisense RNA 1 (MAF...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Ruan Z,Deng H,Liang M,Xu Z,Lai M,Ren H,Deng X,Su X

    更新日期:2020-10-19 00:00:00

  • Long noncoding RNA DLX6-AS1 promotes neuroblastoma progression by regulating miR-107/BDNF pathway.

    abstract:Background:Long noncoding RNAs (lncRNAs) play essential roles in tumor progression. However, the functions and targets of lncRNAs in neuroblastoma (NB) progression still remain to be determined. In this study, we aimed to investigate the effect of lncRNA DLX6 antisense RNA 1 (DLX6-AS1) on NB and the underlying mechanis...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Zhang HY,Xing MQ,Guo J,Zhao JC,Chen X,Jiang Z,Zhang H,Dong Q

    更新日期:2019-11-27 00:00:00

  • Long noncoding RNA ROR promotes breast cancer by regulating the TGF-β pathway.

    abstract:Background:Breast cancer is the leading cause of oncological mortality among women. Efficient detection of cancer cells in an early stage and potent therapeutic agents targeting metastatic tumors are highly needed to improve survival rates. Emerging evidence indicates that lncRNAs (long noncoding RNAs) are critical reg...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Hou L,Tu J,Cheng F,Yang H,Yu F,Wang M,Liu J,Fan J,Zhou G

    更新日期:2018-09-18 00:00:00

  • LncRNA HOXA-AS2 promotes glioblastoma carcinogenesis by targeting miR-885-5p/RBBP4 axis.

    abstract:BACKGROUND:LncRNA HOXA-AS2 has been found in the literature to deteriorate glioblastoma. However, its regulatory mechanism is yet to be fully investigated. Our study focused chiefly on the interaction and role of the HOXA-AS2/miR-885-5p/RBBP4 axis in the development of glioblastoma. METHODS:qRT-PCR analysis was perfor...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Shou J,Gao H,Cheng S,Wang B,Guan H

    更新日期:2021-01-11 00:00:00

  • Real-time RT-PCR analysis of mRNA decay: half-life of Beta-actin mRNA in human leukemia CCRF-CEM and Nalm-6 cell lines.

    abstract::BACKGROUND: We describe an alternative method to determine mRNA half-life (t1/2) based on the Real-Time RT-PCR procedure. This approach was evaluated by using the beta-actin gene as a reference molecule for measuring of mRNA stability. RESULTS: Human leukemia Nalm-6 and CCRF-CEM cells were treated with various concent...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Leclerc GJ,Leclerc GM,Barredo JC

    更新日期:2002-03-07 00:00:00

  • CDK5RAP3 inhibits angiogenesis in gastric neuroendocrine carcinoma by modulating AKT/HIF-1α/VEGFA signaling.

    abstract:Background:Angiogenesis plays critical roles in the progression and metastasis of malignant tumors. Gastric neuroendocrine carcinoma is an uncommon stomach cancer that is rich in blood vessels and exhibits highly malignant biological behavior with a poor prognosis. The role of CDK5RAP3 in GNEC has not been reported to ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Lin JX,Weng XF,Xie XS,Lian NZ,Qiu SL,Wang JB,Lu J,Chen QY,Cao LL,Lin M,Tu RH,Yang YH,Liu SJ,Hu M,Lin YK,Huang CM,Zheng CH,Li P,Xie JW

    更新日期:2019-11-07 00:00:00

  • HIF-2alpha downregulation in the absence of functional VHL is not sufficient for renal cell differentiation.

    abstract:BACKGROUND:Mutational inactivation of the von Hippel-Lindau (VHL) tumor suppressor gene has been linked to hereditary as well as sporadic clear cell renal carcinomas. The product of the VHL gene, pVHL, acts to target hypoxia-inducible factor alpha (HIF-alpha) subunits for ubiquitination and subsequent degradation. Usin...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Hughes MD,Kapllani E,Alexander AE,Burk RD,Schoenfeld AR

    更新日期:2007-06-28 00:00:00

  • OSskcm: an online survival analysis webserver for skin cutaneous melanoma based on 1085 transcriptomic profiles.

    abstract:Background:Cutaneous melanoma is one of the most aggressive and lethal skin cancers. It is greatly important to identify prognostic biomarkers to guide the clinical management. However, it is technically challenging for untrained researchers to process high dimensional profiling data and identify potential prognostic g...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Zhang L,Wang Q,Wang L,Xie L,An Y,Zhang G,Zhu W,Li Y,Liu Z,Zhang X,Tang P,Huo X,Guo X

    更新日期:2020-05-19 00:00:00

  • NEAT1/miR-23a-3p/KLF3: a novel regulatory axis in melanoma cancer progression.

    abstract:Background:Melanoma is an extremely aggressive malignant skin tumor with high mortality. Many types of long noncoding RNAs and microRNAs have been reported to be associated with the oncogenesis of melanoma. However, a novel lncRNA-NEAT has not been thoroughly investigated in melanoma cancer. The purposes of this study ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Ding F,Lai J,Gao Y,Wang G,Shang J,Zhang D,Zheng S

    更新日期:2019-08-22 00:00:00

  • Correction to: Identification of prognosis-related genes and construction of multi-regulatory networks in pancreatic cancer microenvironment by bioinformatics analysis.

    abstract::[This corrects the article DOI: 10.1186/s12935-020-01426-1.]. ...

    journal_title:Cancer cell international

    pub_type: 已发布勘误


    authors: Li T,Liu Q,Zhang R,Liao Q,Zhao Y

    更新日期:2020-08-18 00:00:00

  • Melatonin enhances TNF-α-mediated cervical cancer HeLa cells death via suppressing CaMKII/Parkin/mitophagy axis.

    abstract:Background:Tumor necrosis factor-α (TNF-α) immunotherapy controls the progression of human cervical cancer. Here, we explored the detailed molecular mechanisms played by melatonin in human cervical cancer (HeLa cells) death in the presence of TNF-α injury, with a particular attention to the mitochondrial homeostasis. ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Zhao Q,Wang W,Cui J

    更新日期:2019-03-14 00:00:00

  • MicroRNA-1269 promotes cell proliferation via the AKT signaling pathway by targeting RASSF9 in human gastric cancer.

    abstract:Background:MicroRNAs (miRNAs) play key roles in tumorigenesis and progression of gastric cancer (GC). miR-1269 has been reported to be upregulated in several cancers and plays a crucial role in carcinogenesis and cancer progression. However, the biological function of miR-1269 in human GC and its mechanism remain uncle...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Liu WL,Wang HX,Shi CX,Shi FY,Zhao LY,Zhao W,Wang GH

    更新日期:2019-11-21 00:00:00

  • Protease nexin-1 expression is altered in human breast cancer.

    abstract:BACKGROUND:Urokinase-type Plasminogen Activator (uPA), a serine protease, plays a pivotal role in human breast cancer metastasis by mediating the degradation of extracellular matrix proteins and promoting cell motility. In more advanced breast cancers, uPA activity is significantly up regulated and serves as a prognost...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Candia BJ,Hines WC,Heaphy CM,Griffith JK,Orlando RA

    更新日期:2006-05-31 00:00:00

  • Overexpression of kinesin superfamily members as prognostic biomarkers of breast cancer.

    abstract:Background:Kinesin superfamily (KIFs) has a long-reported significant influence on the initiation, development, and progress of breast cancer. However, the prognostic value of whole family members was poorly done. Our study intends to demonstrate the value of kinesin superfamily members as prognostic biomarkers as well...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Li TF,Zeng HJ,Shan Z,Ye RY,Cheang TY,Zhang YJ,Lu SH,Zhang Q,Shao N,Lin Y

    更新日期:2020-04-15 00:00:00

  • New insights into the association between AXIN2 148 C/T, 1365 C/T, and rs4791171 A/G variants and cancer risk.

    abstract:Background:Many epidemiological studies have investigated association of AXIN2 variants on overall cancer risks; however, the available results remain inconsistent. Methods:An updated analysis was conducted to ascertain a more accurate estimation of the correlation between AXIN2 148 C/T, 1365 C/T, and rs4791171 A/G po...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Xu B,Yuan W,Shi L,Zuo L,Wu XY,Zhang W,Wen Q

    更新日期:2019-05-06 00:00:00

  • Upregulation of microRNA 344a-3p is involved in curcumin induced apoptosis in RT4 schwannoma cells.

    abstract:Background:Schwannoma arising from peripheral nervous sheaths is a benign tumor. Methods:To evaluate cell cytotoxicity, (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction and terminal deoxynucleotidyltransferase UTP nick-end labeling (TUNEL) assays were used. A microRNA (miRNA) array ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Sohn EJ,Bak KM,Nam YK,Park HT

    更新日期:2018-12-04 00:00:00

  • Human colorectal cancer-derived carcinoma associated fibroblasts promote CD44-mediated adhesion of colorectal cancer cells to endothelial cells by secretion of HGF.

    abstract:Background:Carcinoma-associated fibroblasts (CAFs) are dominant components of tumor microenvironment, which has been reported to promote development, progression, and metastasis of cancer. However, the role of CAFs during adhesion process remains unknown. It has been hypothesized that CAFs contribute to adhesion to end...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Zhang R,Qi F,Shao S,Li G,Feng Y

    更新日期:2019-07-24 00:00:00

  • SENP1-mediated deSUMOylation of USP28 regulated HIF-1α accumulation and activation during hypoxia response.

    abstract:Background:The ubiquitin-specific protease 28 (USP28) is an oncogenic deubiquitinase, which plays a critical role in tumorigenesis via antagonizing the ubiquitination and degradation of tumor suppressor protein FBXW7-mediated oncogenic substrates. USP28 controls hypoxia-dependent angiogenesis and metastasis by preventi...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Du SC,Zhu L,Wang YX,Liu J,Zhang D,Chen YL,Peng Q,Liu W,Liu B

    更新日期:2019-01-03 00:00:00

  • Prognostic nutritional index and the prognosis of diffuse large b-cell lymphoma: a meta-analysis.

    abstract:Background:Some studies have investigated the prognostic value exhibited by the Prognostic Nutritional Index (PNI) in patients suffering diffuse large B-cell lymphoma (DLBCL), but varying results were obtained. In order to determine the specific prognostic value more accurately, a meta-analysis was conducted in this st...

    journal_title:Cancer cell international

    pub_type: 杂志文章,评审


    authors: Luan C,Wang F,Wei N,Chen B

    更新日期:2020-09-15 00:00:00

  • The assessment of minimal residual disease versus that of somatic mutations for predicting the outcome of acute myeloid leukemia patients.

    abstract:Background:In addition to morphological and cytogenetic features, acute myeloid leukemias are characterized by mutations that can be used for target-therapy; also the minimal/measurable residual disease (MRD) could be an important prognostic factor. The purpose of this retrospective study was to investigate if somatic ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Salehzadeh S,Guerrini F,Pizzano U,Grassi S,Ciabatti E,Iovino L,Buda G,Caracciolo F,Benedetti E,Orciuolo E,Pelosini M,Consani G,Carulli G,Metelli MR,Martini F,Mazziotta F,Mazzantini E,Rossi P,Tavarozzi R,Ricci F,Pe

    更新日期:2019-04-04 00:00:00

  • Prognostic and clinicopathological significance of long noncoding RNA HOXA11-AS expression in human solid tumors: a meta-analysis.

    abstract:Background:Recent studies have emphasized the important prognostic role of long noncoding RNAs (lncRNAs) in various types of cancers. Here we conducted a meta-analysis to investigate whether lncRNA HOXA11-AS can be served as a prognostic biomarker in human cancers. Patients/methods:We systematically searched PubMed, E...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Mu S,Ai L,Fan F,Sun C,Hu Y

    更新日期:2018-01-03 00:00:00

  • Overexpression of p65 attenuates celecoxib-induced cell death in MDA-MB-231 human breast cancer cell line.

    abstract:BACKGROUND:Celecoxib is a selective cyclooxygenase (COX)-2 inhibitor that has been reported to reduce the risk of breast cancer. In our previous study, celecoxib induced apoptosis and caused cell cycle arrest at the G0/G1 phase in the breast cancer cell line MDA-MB-231, and its effects were mediated by downregulation o...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Wang L,Kang F,Li J,Zhang J,Shan B

    更新日期:2013-02-12 00:00:00

  • Epigallocatechin-3-gallate inhibits the growth and increases the apoptosis of human thyroid carcinoma cells through suppression of EGFR/RAS/RAF/MEK/ERK signaling pathway.

    abstract:Background:Thyroid cancer is the most common type of endocrine malignancy and the incidence rate is rapidly increasing worldwide. Epigallocatechin-3-gallate (EGCG) could suppress cancer growth and induce apoptosis in many types of cancer cells. However, the mechanism of action of EGCG on the growth of human thyroid car...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Wu D,Liu Z,Li J,Zhang Q,Zhong P,Teng T,Chen M,Xie Z,Ji A,Li Y

    更新日期:2019-02-28 00:00:00

  • Curcumin reduces mitomycin C resistance in breast cancer stem cells by regulating Bcl-2 family-mediated apoptosis.

    abstract:BACKGROUND:Curcumin, a natural compound derived from the turmeric rhizome Curcuma longa Linn, has anticancer and chemoresistance reduction biological activities. We evaluated the efficacy of curcumin in sensitizing chemotherapy drugs through regulation of Bcl-2-mediated apoptosis in breast cancer stem-like cells (BCSCs...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Zhou QM,Sun Y,Lu YY,Zhang H,Chen QL,Su SB

    更新日期:2017-09-26 00:00:00

  • Transgelin interacts with PARP1 in human colon cancer cells.

    abstract:Background:Transgelin, an actin-binding protein, is associated with cytoskeleton remodeling. Findings from our previous studies demonstrated that transgelin was up-regulated in node-positive colorectal cancer (CRC) versus node-negative disease. Over-expression of TAGLN affected the expression of 256 downstream transcri...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Lew ZX,Zhou HM,Fang YY,Ye Z,Zhong W,Yang XY,Yu Z,Chen DY,Luo SM,Chen LF,Lin Y

    更新日期:2020-08-03 00:00:00

  • The novel mTOR inhibitor CCI-779 (temsirolimus) induces antiproliferative effects through inhibition of mTOR in Bel-7402 liver cancer cells.

    abstract:BACKGROUND:Liver cancer is one of the most frequent cancers in the world. Targeted therapy of cancer with specific inhibitors is developing and has shown promising antitumor efficacy. CCI-779 (temsirolimus), a specific inhibitor of mTOR (mammalian target of rapamycin), can block the mTOR signaling pathway. Here, we sys...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Li S,Liang Y,Wu M,Wang X,Fu H,Chen Y,Wang Z

    更新日期:2013-03-28 00:00:00

  • TP53 mutant cell lines selected for resistance to MDM2 inhibitors retain growth inhibition by MAPK pathway inhibitors but a reduced apoptotic response.

    abstract:Background:Emergence of resistance to molecular targeted therapy constitutes a limitation to clinical benefits in cancer treatment. Cross-resistance commonly happens with chemotherapeutic agents but might not with targeted agents. Methods:In the current study, TP53 wild-type cell lines with druggable MAPK pathway muta...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Wu CE,Koay TS,Ho YH,Lovat P,Lunec J

    更新日期:2019-03-07 00:00:00

  • The role of A20 in the pathogenesis of lymphocytic malignancy.

    abstract:: Autoimmune phenomena were identified in many different cases of hematological diseases and solid tumors, which may be due to alterations in the expression and function of the NF-κB signaling pathway. Recently, a number of studies have shown that the deletion or mutation of A20, a negative regulator of NF-κB, is frequ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Zhang F,Yang L,Li Y

    更新日期:2012-11-07 00:00:00