当前位置： SCI文献检索 > Cancer Cell International期刊下所有文献 > Casticin inhibits nasopharyngeal carcinoma growth by targeting phosphoinositide 3-kinase.

Casticin inhibits nasopharyngeal carcinoma growth by targeting phosphoinositide 3-kinase.

Abstract：

Background:Casticin, an isoflavone compound extracted from the herb Fructus Viticis, has demonstrated anti-inflammatory and anticancer activities and properties. The aim of this study was to investigate the effects and mechanisms of casticin in nasopharyngeal carcinoma (NPC) cells and to determine its potential for targeted use as a medicine. Methods:NPC cells were used to perform the experiments. The CCK‑8 assay and colony formation assays were used to assess cell viability. Flow cytometry was used to measure the cell cycle and apoptosis analysis (annexin V/PI assay). A three-dimensional (3D) tumour sphere culture system was used to characterize the effect of casticin on NPC stem cells. In silico molecular docking prediction and high-throughput KINOME scan assays were used to evaluate the binding of casticin to phosphoinositide 3-kinase (PI3K), including wild-type and most of mutants variants. We also used the SelectScreen assay to detect the IC50 of ATP activity in the active site of the target kinase. Western blotting was used to evaluate the changes in key proteins involved cell cycle, apoptosis, stemness, and PI3K/protein kinase B (AKT) signalling. The effect of casticin treatment in vivo was determined by using a xenograft mouse model. Results:Our results indicate that casticin is a new and novel selective PI3K inhibitor that can significantly inhibit NPC proliferation and that it induces G2/GM arrest and apoptosis by upregulating Bax/BCL2 expression. Moreover, casticin was observed to affect the self-renewal ability of the nasopharyngeal carcinoma cell lines, and a combination of casticin with BYL719 was observed to induce a decrease in the level of the phosphorylation of mTORC1 downstream targets in BYL719-insensitive NPC cell lines. Conclusion:Casticin is a newly emerging selective PI3K inhibitor with potential for use as a targeted therapeutic treatment for nasopharyngeal carcinoma. Accordingly, casticin might represent a novel and effective agent against NPC and likely has high potential for combined use with pharmacological agents targeting PI3K/AKT.

journal_name

Cancer Cell Int

journal_title

Cancer cell international

authors

Liu J,Yang J,Hou Y,Zhu Z,He J,Zhao H,Ye X,Li D,Wu Z,Huang Z,Hao B,Yao K

doi

10.1186/s12935-019-1069-6

subject

Has Abstract

pub_date

2019-12-21 00:00:00

pages

348

issn

1475-2867

pii

1069

journal_volume

pub_type

杂志文章

在线工具

Casticin inhibits nasopharyngeal carcinoma growth by targeting phosphoinositide 3-kinase.