Abstract:
Background:Breast cancer has been the first death cause of cancer in women all over the world. Metastasis is believed to be the most important process for treating breast cancer. There is evidence that lncRNA MEG3 functions as a tumor suppressor in breast cancer metastasis. However, upstream regulation of MEG3 in breast cancer remain elusive. Therefore, it is critical to elucidate the underlying mechanism upstream MEG3 to regulate breast cancer metastasis. Methods:We employed RT-qPCR and Western blot to examine expression level of miR-506, DNMT1, SP1, SP3 and MEG3. Besides, methylation-specific PCR was used to determine the methylation level of MEG3 promoter. Wound healing assay and transwell invasion assay were utilized to measure migration and invasion ability of breast cancer cells, respectively. Results:SP was upregulated while miR-506 and MEG3 were downregulated in breast tumor tissue compared to adjacent normal breast tissues. In addition, we found that miR-506 regulated DNMT1 expression in an SP1/SP3-dependent manner, which reduced methylation level of MEG3 promoter and upregulated MEG3 expression. SP3 knockdown or miR-506 mimic suppressed migration and invasion of MCF-7 and MDA-MB-231 cells whereas overexpression of SP3 compromised miR-506-inhibited migration and invasion. Conclusions:Our data reveal a novel axis of miR-506/SP3/SP1/DNMT1/MEG3 in regulating migration and invasion of breast cancer cell lines, which provide rationales for developing effective therapies to treating metastatic breast cancers.
journal_name
Cancer Cell Intjournal_title
Cancer cell internationalauthors
Wang XX,Guo GC,Qian XK,Dou DW,Zhang Z,Xu XD,Duan X,Pei XHdoi
10.1186/s12935-018-0642-8subject
Has Abstractpub_date
2018-10-25 00:00:00pages
171issn
1475-2867pii
642journal_volume
18pub_type
杂志文章abstract:Background:Emergence of resistance to molecular targeted therapy constitutes a limitation to clinical benefits in cancer treatment. Cross-resistance commonly happens with chemotherapeutic agents but might not with targeted agents. Methods:In the current study, TP53 wild-type cell lines with druggable MAPK pathway muta...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-0768-3
更新日期:2019-03-07 00:00:00
abstract:Background:Drug resistance to 5-fluorouracil (5-FU) and recurrence after chemotherapy in colorectal cancer remain a challenge to be resolved for the improvement of patient outcomes. It is recognized that a variety of secretory proteins released from the tumor cells exposed to chemo-drugs into the tumor microenvironment...
journal_title:Cancer cell international
pub_type: 杂志文章
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更新日期:2020-09-14 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-14-38
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-13-54
更新日期:2013-06-01 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-015-0265-2
更新日期:2015-12-10 00:00:00
abstract::BACKGROUND: Mast cells are known to accumulate at sites of inflammation and upon activation to release their granule content, e.g. histamine, cytokines and proteases. The secretory leukocyte protease inhibitor (SLPI) is produced in the respiratory mucous and plays a role in regulating the activity of the proteases. RE...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-3-14
更新日期:2003-08-20 00:00:00
abstract::[This corrects the article DOI: 10.1186/s12935-020-01426-1.]. ...
journal_title:Cancer cell international
pub_type: 已发布勘误
doi:10.1186/s12935-020-01482-7
更新日期:2020-08-18 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-017-0452-4
更新日期:2017-10-24 00:00:00
abstract:BACKGROUND:Increased expression of voltage-gated sodium channels (VGSCs) have been implicated with strong metastatic potential of human breast cancer in vitro and in vivo where the main culprits are cardiac isoform Nav1.5 and its 'neonatal' splice variant, nNav1.5. Several factors have been associated with Nav1.5 and n...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-017-0442-6
更新日期:2017-08-01 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-0761-x
更新日期:2019-02-28 00:00:00
abstract:BACKGROUND:Several reports have revealed that cancer stem cells (CSCs) exist in many types of solid tumors. Some studies have demonstrated that side population (SP) cells isolated from diverse cancer lines harbor cancer stem-like properties, but there are few reports examining the characteristic of SP cells in human ce...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-14-3
更新日期:2014-01-13 00:00:00
abstract:Background:Lymph node metastasis is one of the most important factors affecting the prognosis of tongue cancer, and the molecular mechanism regulating lymph node metastasis of tongue cancer is poorly known. Methods:The gene expression dataset GSE2280 and The Cancer Genome Atlas (TCGA) tongue cancer dataset were downlo...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-1028-2
更新日期:2019-11-21 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-015-0206-0
更新日期:2015-05-23 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-5-20
更新日期:2005-07-07 00:00:00
abstract::[This corrects the article DOI: 10.1186/s12935-019-0742-0.]. ...
journal_title:Cancer cell international
pub_type: 杂志文章,已发布勘误
doi:10.1186/s12935-019-0797-y
更新日期:2019-04-11 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01623-y
更新日期:2020-11-03 00:00:00
abstract:BACKGROUND:Non-small cell lung cancer comprises the majority of lung cancer cases and is insensitive to chemotherapy. Most patients develop drug resistance. Recently, tetrandrine (TET), a bis-benzylisoquinoline alkaloid, was identified as a novel anti-cancer agent. However, the effect of tetrandrine combined with cispl...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-017-0410-1
更新日期:2017-03-27 00:00:00
abstract::Application of novel methods in cancer therapy is important in terms of management and treatment of the life-threatening disorder. It appears that autophagy is a potential target in cancer therapy, as a variety of drugs targeting autophagy have shown great potential in reducing the viability and proliferation of cance...
journal_title:Cancer cell international
pub_type: 杂志文章,评审
doi:10.1186/s12935-020-01634-9
更新日期:2020-11-04 00:00:00
abstract::Amongst males, leukaemia is the most common cause of cancer-related death in individuals younger than 40 years of age whereas in female children and adolescents, leukaemia is the most common cause of cancer-related death. Chronic myeloid leukaemia (CML) is a chronic leukaemia of the haematopoietic stem cells affecting...
journal_title:Cancer cell international
pub_type: 杂志文章,评审
doi:10.1186/s12935-017-0460-4
更新日期:2017-10-30 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-014-0133-5
更新日期:2014-11-30 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-0840-z
更新日期:2019-05-06 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01268-x
更新日期:2020-05-24 00:00:00
abstract:Background:Neuroblastoma (NB) is the most common malignant tumor originating from the extracranial sympathetic nervous system in children. The molecular mechanisms underlying this disease are complex, and not completely understood. Methods:Quantitative real-time PCR (qRT-PCR) was applied to quantify the expression of ...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-017-0499-2
更新日期:2018-01-04 00:00:00
abstract:BACKGROUND:Human umbilical cord blood derived-mesenchymal stem cells (hUCMSCs) offer an attractive alternative to bone marrow-derived MSCs (BMMSCs) for cell-based therapy as it is a less invasive source of biological material. However, limited studies have been conducted with hUCMSCs as compared to BMMSCs. The present ...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-014-0093-9
更新日期:2014-09-24 00:00:00
abstract:Background:Increasing evidences have underlined the importance of long non-coding RNAs (lncRNAs) in human malignancies. LINC00958 has been found involved in some cancers. However, the underlying mechanical performance of LINC00958 in lung adenocarcinoma (LAD) has not been explored yet. Methods:The expression of releva...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-1099-0
更新日期:2020-01-23 00:00:00
abstract:BACKGROUND:Advanced cancer and chemotherapy are both associated with immune system suppression. We initiated a clinical trial in patients receiving chemotherapy for metastatic colorectal cancer to determine if administration of GM-CSF in this setting was immunostimulatory. METHODS:Between June, 2003 and January, 2007,...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-12-2
更新日期:2012-01-23 00:00:00
abstract:Background:miR-429 and TLN1 have been shown to affect the biological behaviours of many carcinomas. However, their effects in nasopharyngeal carcinoma (NPC) are not yet clear. Here, we investigated their regulatory relationships and effects on NPC cells. Methods:TargetScan was used to predict the regulatory relationsh...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-0831-0
更新日期:2019-04-30 00:00:00
abstract:Background:Growing evidence suggests that MiRNAs play essential roles in the initiation and progression of colorectal cancer (CRC). The aberrant expression of miR-384 has been reported in some cancers. However, the role and mechanism of miR-384 in CRC proliferation remains unknown. Methods:The expression of miR-384 wa...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-018-0628-6
更新日期:2018-09-03 00:00:00
abstract::Non-small-cell lung cancer (NSCLC) is a type of malignancy with progressive metastasis having poor prognosis and lowered survival resulting from late diagnosis. The therapeutic approaches for the treatment of this incurable cancer are chemo- and radiotherapy. Since current treatments are insufficient and because of dr...
journal_title:Cancer cell international
pub_type: 杂志文章,评审
doi:10.1186/s12935-019-0853-7
更新日期:2019-05-16 00:00:00
abstract:Background:Melanoma is the most aggressive type of skin cancer with high mortality rate and poor prognosis. lncRNA MEG3, a tumor suppressor, is closely related to the development of various cancers. However, the role of lncRNA MEG3 in melanoma has seldom been studied. Methods:RT-PCR was used to examine the expressions...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-1087-4
更新日期:2020-01-10 00:00:00