Abstract：
BACKGROUND:The goal of this study was to identify the nature of the inclusion bodies that have been found in HeLa cells (cervical cancer immortal cell line) by electron microscope and to determine whether the major capsid protein (L1) of human papillomavirus (HPV) can be expressed in HPV-positive uterine cervix cancer cells. METHODS:HPV L1 protein expression in HeLa cells was detected with anti-HPV L1 multivalent mice monoclonal antibody and rabbit polyclonal anti-HPV L1 antibody by ELISA, light microscope immunohistochemistry, electron microscope immunocytochemistry and Western blotting assays. Reverse transcriptional PCR (RT-PCR) was performed to detect the transcription of L1 mRNA in HeLa cells. The immortalized human keratinocyte HeCat was used as the negative control. RESULTS:HPV L1 proteins reacted positively in the lysate of HeLa cells by ELISA assays. HRP labeled light microscope immunohistochemistry assay showed that there was a strong HPV L1 positive reaction in HeLa cells. Under the electron microscope, irregular shaped inclusion bodies, assembled by many small and uniform granules, had been observed in the cytoplasm of some HeLa cells. These granules could be labeled by the colloidal gold carried by HPV L1 antibody. The Western blotting assay showed that there was a L1 reaction strap at 80-85 kDa in the HeLa cell lysates, hence demonstrating the existence of HPV18 L1 in HeLa cells. RT-PCR assay showed that the L1 mRNA was transcribed in HeLa cells. CONCLUSIONS:The inclusion bodies found in the cytoplasm of HeLa cells are composed of HPV18 L1 protein. Since HeLa cell line is a type of cervical cancer cells, this implies that HeLa cells have the ability to express HPV L1 proteins.
journal_name
Cancer Cell Intjournal_title
Cancer cell internationalauthors
Xiao CY,Fu BB,Li ZY,Mushtaq G,Kamal MA,Li JH,Tang GC,Xiao SSdoi
10.1186/s12935-015-0206-0subject
Has Abstractpub_date
2015-05-23 00:00:00pages
53issn
1475-2867pii
206journal_volume
15pub_type
杂志文章abstract：Background:Glioma is the most common highly aggressive, primary adult brain tumour. Clinical data show that therapeutic approaches cannot reach the expectations in patients, thus gliomas are mainly incurable diseases. Tumour cells can adapt rapidly to alterations during therapeutic treatments related to their metabolic...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-018-0710-0
更新日期：2018-12-19 00:00:00
abstract：BACKGROUND:The aim of this study is to explore the expression of alpha-synuclein (α-synuclein) in benign, atypical, and anaplastic meningiomas and determine its role in the malignant progression of meningiomas. METHODS:Expression of α-synuclein was measured in 44 meningioma samples by real-time PCR analysis. The effec...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-016-0361-y
更新日期：2016-11-15 00:00:00
abstract：BACKGROUND:Circular RNAs (circRNAs) is a newly discovered type of non-coding RNA, the abnormal expression of which has been demonstrated in many types of human tumors. So they have been considered as promising candidates as diagnostic and therapeutic targets in cancer. This research aimed to screen the profile of circR...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01681-2
更新日期：2021-01-07 00:00:00
abstract：Background:New drugs for adrenocortical carcinoma (ACC) are needed because most patients undergo rapid disease progression despite surgery and adjuvant therapy with mitotane. In this study, we aimed to investigate the in vitro effects of different chemotherapy drugs, alone or combined with mitotane, on the viability of...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-018-0527-x
更新日期：2018-03-01 00:00:00
abstract：Background:Sarcomas are malignant heterogeneous tumors of mesenchymal derivation. Dedifferentiated liposarcoma (DDLPS) is aggressive with recurrence in 80% and metastasis in 20% of patients. We previously found that miR-133a was significantly underexpressed in liposarcoma tissues. As this miRNA has recently been shown ...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-018-0583-2
更新日期：2018-06-26 00:00:00
abstract：Background:Oxidative stress is responsible for some alterations in the chemical structure and, consequently, in the function of proteins, lipids, and DNA. Recent studies have linked oxidative stress to cancers, particularly thyroid cancer, but the mechanisms remain unclear. Here, we further characterize the role of oxi...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-018-0504-4
更新日期：2018-01-15 00:00:00
abstract：Background:Tumor necrosis factor-α (TNF-α) immunotherapy controls the progression of human cervical cancer. Here, we explored the detailed molecular mechanisms played by melatonin in human cervical cancer (HeLa cells) death in the presence of TNF-α injury, with a particular attention to the mitochondrial homeostasis. ...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-019-0777-2
更新日期：2019-03-14 00:00:00
abstract：Background:In recent years, the B cell receptor (BCR) signaling pathway has become a "hot point" because it plays a critical role in B-cell proliferation and function. Bruton's tyrosine kinase (BTK) is overexpressed in many subtypes of B-cell lymphoma as a downstream kinase in the BCR signaling pathway. Ibrutinib, the ...
journal_title：Cancer cell international
pub_type： 杂志文章,评审
doi：10.1186/s12935-020-01518-y
更新日期：2020-09-29 00:00:00
abstract：Background:Thyroid cancer (TC) is the most frequent endocrine malignancy. Long noncoding RNAs (lncRNAs) have been confirmed to act as significant roles in tumor development. The role of lncRNA TMPO-AS1 in TC is still unclear, so it remains to be explored. The aim of the research is to investigate the role and regulator...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01334-4
更新日期：2020-07-08 00:00:00
abstract：:Colorectal cancer (CRC) is a complex and molecularly heterogeneous disease representing one of the most frequent causes of cancer-related death worldwide. About 8-15% of CRCs harbor a mutation in BRAF gene, a proto-oncogene involved in cell proliferation, differentiation and survival through the MAPK signaling cascade...
journal_title：Cancer cell international
pub_type： 杂志文章,评审
doi：10.1186/s12935-020-1117-2
更新日期：2020-01-29 00:00:00
abstract：:[This corrects the article DOI: 10.1186/s12935-017-0407-9.]. ...
journal_title：Cancer cell international
pub_type： 已发布勘误
doi：10.1186/s12935-020-01375-9
更新日期：2020-07-17 00:00:00
abstract：Background:At the present time, colorectal cancer (CRC) is still known as a disease with a high mortality rate. Theranostics are flawless scenarios that link diagnosis with therapy, including precision medicine as a critical platform that relies on the development of biomarkers particularly "liquid biopsy". Circulating...
journal_title：Cancer cell international
pub_type： 杂志文章,评审
doi：10.1186/s12935-020-01389-3
更新日期：2020-07-06 00:00:00
abstract：BACKGROUND:The expression of major histocompatibility complex class II (MHCII) antigens in both mouse and human tumors is rare, and these antigens are not easily inducible by IFN-gamma (IFNg). Since MHCII may play an important role in the development of host antitumor immune response, we explored the possibility of res...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/1475-2867-3-4
更新日期：2003-03-25 00:00:00
abstract：BACKGROUND:Cancer cells, even in the presence of available oxygen, have a glycolysis enhancement. The "aerobic glycolysis" is known as the Warburg effect and it is considered one of the fundamental hallmarks of metabolic alteration during malignant transformation. A feature of many tumors is also a change into ions equ...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-014-0077-9
更新日期：2014-08-12 00:00:00
abstract：Background:Long non-coding RNA (lncRNA) BM742401 is a tumor suppressor in gastric cancer and chronic lymphocytic leukemia. As the promoter and coding region of BM742401 are fully embedded in a CpG island, we hypothesized that BM742401 is a tumor suppressor lncRNA epigenetically silenced by promoter DNA methylation in m...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01504-4
更新日期：2020-08-25 00:00:00
abstract：BACKGROUND:Breast cancer (BrCa) is the most common female malignancy worldwide and has the highest morbidity among all cancers in females. Unfortunately, the mechanisms of BrCa growth and metastasis, which lead to a poor prognosis in BrCa patients, have not been well characterized. METHODS:Immunohistochemistry (IHC) w...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01733-7
更新日期：2021-01-06 00:00:00
abstract：UNLABELLED: BACKGROUND:Anticancer research resulted in the discovery of a promising antimitotic metabolite, 2-methoxyestradiol. 2-Methoxyestradiol-bis-sulphamate, a bis-sulphamoylated analogue exerts antiproliferative- and antimitotic activity. Investigating the anticancer potential of 2-methoxyestradiol-bis-sulphamat...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/1475-2867-12-37
更新日期：2012-08-20 00:00:00
abstract：BACKGROUND:Advanced cancer and chemotherapy are both associated with immune system suppression. We initiated a clinical trial in patients receiving chemotherapy for metastatic colorectal cancer to determine if administration of GM-CSF in this setting was immunostimulatory. METHODS:Between June, 2003 and January, 2007,...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/1475-2867-12-2
更新日期：2012-01-23 00:00:00
abstract：:Application of novel methods in cancer therapy is important in terms of management and treatment of the life-threatening disorder. It appears that autophagy is a potential target in cancer therapy, as a variety of drugs targeting autophagy have shown great potential in reducing the viability and proliferation of cance...
journal_title：Cancer cell international
pub_type： 杂志文章,评审
doi：10.1186/s12935-020-01634-9
更新日期：2020-11-04 00:00:00
abstract：BACKGROUND:The aim of this study was to determine whether brain abundant membrane attached signal protein 1 (BASP1) is a valuable prognostic biomarker for cervical cancer and whether BASP1 regulates the progression of cervical cancer. METHODS:Quantitative real-time PCR, western blotting, and immunohistochemistry were ...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-017-0452-4
更新日期：2017-10-24 00:00:00
abstract：BACKGROUND:Prostate cancer is a leading cause of male cancer specific mortality. When cure by radical prostatectomy is not possible the next line of prostate cancer treatment is androgen deprivation. However prolonged androgen deprivation often results in relapse and androgen-independent prostate cancer that is inevita...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/1475-2867-8-3
更新日期：2008-03-18 00:00:00
abstract：Background:Despite improvements in nasopharyngeal carcinoma (NPC) treatment, patients with recurrence and metastasis still have a poor prognosis. Thus, the identification of novel biomarkers is urgently needed to predict outcomes and tailor treatment for NPC. Methods:Four data sets were downloaded from Gene Expression...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01587-z
更新日期：2020-10-12 00:00:00
abstract：Background:Laryngopharyngeal reflux (LPR), with its increasing morbidity, is attracting considerable attention. In recent years, the causal role between LPR and laryngeal carcinoma has been debated. The main harmful component of LPR is pepsin, which has been shown to induce mucosal inflammation by damaging the mucous m...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-019-0772-7
更新日期：2019-03-20 00:00:00
abstract：Background:Although major driver gene have been identified, the complex molecular heterogeneity of renal cell cancer (RCC) remains unclear. Therefore, more relevant genes need to be identified to explain the pathogenesis of renal cancer. Methods:Microarray datasets GSE781, GSE6344, GSE53000 and GSE68417 were downloade...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01405-6
更新日期：2020-07-21 00:00:00
abstract：Background:Some studies have investigated the prognostic value exhibited by the Prognostic Nutritional Index (PNI) in patients suffering diffuse large B-cell lymphoma (DLBCL), but varying results were obtained. In order to determine the specific prognostic value more accurately, a meta-analysis was conducted in this st...
journal_title：Cancer cell international
pub_type： 杂志文章,评审
doi：10.1186/s12935-020-01535-x
更新日期：2020-09-15 00:00:00
abstract：:[This corrects the article DOI: 10.1186/s12935-019-0742-0.]. ...
journal_title：Cancer cell international
pub_type： 杂志文章,已发布勘误
doi：10.1186/s12935-019-0797-y
更新日期：2019-04-11 00:00:00
abstract：:Osteosarcoma (OS) is one of the most prevalent malignant cancers with lower survival and poor overall prognosis mainly in children and adolescents. Identifying the molecular mechanisms and OS stem cells (OSCs) as new concepts involved in disease pathogenesis and progression may potentially lead to new therapeutic targ...
journal_title：Cancer cell international
pub_type： 杂志文章,评审
doi：10.1186/s12935-018-0654-4
更新日期：2018-10-16 00:00:00
abstract：BACKGROUND:Prostate cancer progression to androgen independence is the primary cause of mortality by this tumor type. The IGF-1/IGF-1R axis is well known to contribute to prostate cancer initiation, but its contribution to invasiveness and the downstream signalling mechanisms that are involved are unclear at present. ...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/1475-2867-8-10
更新日期：2008-07-03 00:00:00
abstract：Background:This study aims to investigate the role of microRNA-92a (miR-92a) in metastasis of osteosarcoma (OS) cells in vivo and in vitro by regulatingTCF21 with the transmission of bone marrow derived mesenchymal stem cells (BMSCs). Methods:BMSCs were isolated, purified and cultured from healthy adult bone marrow ti...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-019-0741-1
更新日期：2019-02-12 00:00:00
abstract：Background and aims:microRNAs (miRNAs) have been reported to regulate proliferation and migration by down-regulating the expression of target genes. The aims of this study were to investigate whether miR-4316 inhibited proliferation and migration by downregulating vascular endothelial growth factor A (VEGF-A) and its c...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-1132-3
更新日期：2020-02-22 00:00:00
