Observations on the expression of human papillomavirus major capsid protein in HeLa cells.


BACKGROUND:The goal of this study was to identify the nature of the inclusion bodies that have been found in HeLa cells (cervical cancer immortal cell line) by electron microscope and to determine whether the major capsid protein (L1) of human papillomavirus (HPV) can be expressed in HPV-positive uterine cervix cancer cells. METHODS:HPV L1 protein expression in HeLa cells was detected with anti-HPV L1 multivalent mice monoclonal antibody and rabbit polyclonal anti-HPV L1 antibody by ELISA, light microscope immunohistochemistry, electron microscope immunocytochemistry and Western blotting assays. Reverse transcriptional PCR (RT-PCR) was performed to detect the transcription of L1 mRNA in HeLa cells. The immortalized human keratinocyte HeCat was used as the negative control. RESULTS:HPV L1 proteins reacted positively in the lysate of HeLa cells by ELISA assays. HRP labeled light microscope immunohistochemistry assay showed that there was a strong HPV L1 positive reaction in HeLa cells. Under the electron microscope, irregular shaped inclusion bodies, assembled by many small and uniform granules, had been observed in the cytoplasm of some HeLa cells. These granules could be labeled by the colloidal gold carried by HPV L1 antibody. The Western blotting assay showed that there was a L1 reaction strap at 80-85 kDa in the HeLa cell lysates, hence demonstrating the existence of HPV18 L1 in HeLa cells. RT-PCR assay showed that the L1 mRNA was transcribed in HeLa cells. CONCLUSIONS:The inclusion bodies found in the cytoplasm of HeLa cells are composed of HPV18 L1 protein. Since HeLa cell line is a type of cervical cancer cells, this implies that HeLa cells have the ability to express HPV L1 proteins.


Cancer Cell Int


Xiao CY,Fu BB,Li ZY,Mushtaq G,Kamal MA,Li JH,Tang GC,Xiao SS




Has Abstract


2015-05-23 00:00:00










  • Increase of IFN-γ and TNF-α production in CD107a + NK-92 cells co-cultured with cervical cancer cell lines pre-treated with the HO-1 inhibitor.

    abstract:BACKGROUND:Natural killer (NK) cells eliminate virus-infected and tumor cells through the release of perforins and granzymes; they also produce Interferon gamma (IFN-γ) and Tumor necrosis factor alpha (TNF-α), which induce apoptosis in target cells. Many tumors express Heme oxygenase 1 (HO-1), and this expression has b...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Gómez-Lomelí P,Bravo-Cuellar A,Hernández-Flores G,Jave-Suárez LF,Aguilar-Lemarroy A,Lerma-Díaz JM,Domínguez-Rodríguez JR,Sánchez-Reyes K,Ortiz-Lazareno PC

    更新日期:2014-10-01 00:00:00

  • Correction to: miR-484 suppresses proliferation and epithelial-mesenchymal transition by targeting ZEB1 and SMAD2 in cervical cancer cells.

    abstract::[This corrects the article DOI: 10.1186/s12935-017-0407-9.]. ...

    journal_title:Cancer cell international

    pub_type: 已发布勘误


    authors: Hu Y,Xie H,Liu Y,Liu W,Liu M,Tang H

    更新日期:2020-07-17 00:00:00

  • Targeting different types of human meningioma and glioma cells using a novel adenoviral vector expressing GFP-TRAIL fusion protein from hTERT promoter.

    abstract:OBJECTIVE:The objective of this study was to evaluate the anti-tumor effects of Ad/gTRAIL (an adenoviral vector in which expression of GFP and TRAIL is driven by a human telomerase reverse transcriptase promoter, hTERT) on malignant meningiomas and gliomas. BACKGROUND:Gliomas and meningiomas are the two most common ty...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Li JT,Bian K,Zhang AL,Kim DH,Ashley WW,Nath R,McCutcheon I,Fang B,Murad F

    更新日期:2011-10-28 00:00:00

  • CircNEIL3 promotes cervical cancer cell proliferation by adsorbing miR-137 and upregulating KLF12.

    abstract:BACKGROUND:CircRNAs play crucial roles in multiple tumours. However, the functions of most circRNAs in cervical cancer remain unclear. METHODS:This study collected GSE113696 data from the GEO database to search for differentially expressed circRNAs in cervical cancer. Quantitative reverse transcription PCR was used to...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Chen Y,Geng Y,Huang J,Xi D,Xu G,Gu W,Shao Y

    更新日期:2021-01-07 00:00:00

  • Upregulation of microRNA 344a-3p is involved in curcumin induced apoptosis in RT4 schwannoma cells.

    abstract:Background:Schwannoma arising from peripheral nervous sheaths is a benign tumor. Methods:To evaluate cell cytotoxicity, (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction and terminal deoxynucleotidyltransferase UTP nick-end labeling (TUNEL) assays were used. A microRNA (miRNA) array ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Sohn EJ,Bak KM,Nam YK,Park HT

    更新日期:2018-12-04 00:00:00

  • miR-429 suppresses cell proliferation, migration and invasion in nasopharyngeal carcinoma by downregulation of TLN1.

    abstract:Background:miR-429 and TLN1 have been shown to affect the biological behaviours of many carcinomas. However, their effects in nasopharyngeal carcinoma (NPC) are not yet clear. Here, we investigated their regulatory relationships and effects on NPC cells. Methods:TargetScan was used to predict the regulatory relationsh...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Wang Z,Zhu Z,Lin Z,Luo Y,Liang Z,Zhang C,Chen J,Peng P

    更新日期:2019-04-30 00:00:00

  • Functional polymorphisms in LncRNA HOTAIR contribute to susceptibility of pancreatic cancer.

    abstract:Background:Pancreatic cancer (PC) remains one of the most aggressive cancers worldwide. However, genetic factors underlying PC susceptibility remain largely unclear. Long noncoding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR) acts as an oncogene and its genetic variation has been linked to many cancers. However, ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Jiang D,Xu L,Ni J,Zhang J,Cai M,Shen L

    更新日期:2019-02-28 00:00:00

  • Par6 is an essential mediator of apoptotic response to transforming growth factor beta in NMuMG immortalized mammary cells.

    abstract:BACKGROUND:We previously observed that the TGFbeta-Par6 pathway mediates loss of polarity and apoptosis in NMuMG cells. Here we investigate the contribution of Par6 versus TGFbeta receptor I activation to TGFbeta-induced apoptosis in association with changes in apico-basal polarity. We focus on the effect of Par6 activ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Avery-Cooper G,Doerr M,Gilbert RW,Youssef M,Richard A,Huether P,Viloria-Petit AM

    更新日期:2014-03-01 00:00:00

  • Autophagy regulation using luteolin: new insight into its anti-tumor activity.

    abstract::Application of novel methods in cancer therapy is important in terms of management and treatment of the life-threatening disorder. It appears that autophagy is a potential target in cancer therapy, as a variety of drugs targeting autophagy have shown great potential in reducing the viability and proliferation of cance...

    journal_title:Cancer cell international

    pub_type: 杂志文章,评审


    authors: Ashrafizadeh M,Ahmadi Z,Farkhondeh T,Samarghandian S

    更新日期:2020-11-04 00:00:00

  • Association between underexpression of microrna-203 and clinicopathological significance in hepatocellular carcinoma tissues.

    abstract:BACKGROUND:Although recent studies have shown the utility of miR-203 as a cancer-relevant biomarker, the validated clinical significance of miR-203 in HCC remains obscure. The aim of the present study was to evaluate the relationship between miR-203 expression and clinicopathological features in HCC patients. METHODS:...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Liu Y,Ren F,Rong M,Luo Y,Dang Y,Chen G

    更新日期:2015-06-18 00:00:00

  • miR-206 as a prognostic and sensitivity biomarker for platinum chemotherapy in epithelial ovarian cancer.

    abstract:BACKGROUND:Drug resistance is a major obstacle to successful chemotherapy for epithelial ovarian cancer (EOC). We found a subset of miRNAs associated with the response to first-line platinum-based chemotherapy in EOC by microarray, and miR-206 was one of the most significant miRNAs. The purposes of this study were to e...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Yu X,Zhang X,Wang G,Wang B,Ding Y,Zhao J,Liu H,Cui S

    更新日期:2020-11-03 00:00:00

  • Human mast cells decrease SLPI levels in type II - like alveolar cell model, in vitro.

    abstract::BACKGROUND: Mast cells are known to accumulate at sites of inflammation and upon activation to release their granule content, e.g. histamine, cytokines and proteases. The secretory leukocyte protease inhibitor (SLPI) is produced in the respiratory mucous and plays a role in regulating the activity of the proteases. RE...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Hollander C,Nyström M,Janciauskiene S,Westin U

    更新日期:2003-08-20 00:00:00

  • Avenanthramide-C reduces the viability of MDA-MB-231 breast cancer cells through an apoptotic mechanism.

    abstract:BACKGROUND:Avenanthramides (AVN) are a relatively unstudied family of phytochemicals that could be novel chemotherapeutics. These compounds, found in oats, are non-toxic to healthy cells and have been shown to reduce viability of human colon and liver cancers in vitro. However, these studies do not elucidate a molecula...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Hastings J,Kenealey J

    更新日期:2017-10-18 00:00:00

  • LncRNA NEAT1 promotes proliferation, migration, invasion and epithelial-mesenchymal transition process in TGF-β2-stimulated lens epithelial cells through regulating the miR-486-5p/SMAD4 axis.

    abstract:BACKGROUND:Abnormal proliferation, metastasis and epithelial-mesenchymal transformation (EMT) of lens epithelial cells (LECs) are direct factors of posterior capsular opacification (PCO). Nuclear enriched abundant transcript 1 (NEAT1) has been shown to promote cell proliferation, metastasis and EMT, but whether it affe...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Wang H,Zheng G

    更新日期:2020-10-31 00:00:00

  • The assessment of minimal residual disease versus that of somatic mutations for predicting the outcome of acute myeloid leukemia patients.

    abstract:Background:In addition to morphological and cytogenetic features, acute myeloid leukemias are characterized by mutations that can be used for target-therapy; also the minimal/measurable residual disease (MRD) could be an important prognostic factor. The purpose of this retrospective study was to investigate if somatic ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Salehzadeh S,Guerrini F,Pizzano U,Grassi S,Ciabatti E,Iovino L,Buda G,Caracciolo F,Benedetti E,Orciuolo E,Pelosini M,Consani G,Carulli G,Metelli MR,Martini F,Mazziotta F,Mazzantini E,Rossi P,Tavarozzi R,Ricci F,Pe

    更新日期:2019-04-04 00:00:00

  • Role of microRNA-92a in metastasis of osteosarcoma cells in vivo and in vitro by inhibiting expression of TCF21 with the transmission of bone marrow derived mesenchymal stem cells.

    abstract:Background:This study aims to investigate the role of microRNA-92a (miR-92a) in metastasis of osteosarcoma (OS) cells in vivo and in vitro by regulatingTCF21 with the transmission of bone marrow derived mesenchymal stem cells (BMSCs). Methods:BMSCs were isolated, purified and cultured from healthy adult bone marrow ti...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Cao S,Jiang L,Shen L,Xiong Z

    更新日期:2019-02-12 00:00:00

  • Differential effects of HIF-α isoforms on apoptosis in renal carcinoma cell lines.

    abstract:BACKGROUND:Germline mutations in the von Hippel-Lindau (VHL) tumor suppressor gene predispose individuals to clear cell renal carcinomas, hemangioblastomas, and pheochromocytomas. The VHL gene product forms an ubiquitin E3 ligase complex, with regulation of hypoxia-inducible factor alpha (HIF-α) as its best known funct...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Doonachar A,Gallo MD,Doukas D,Pasricha R,Lantsberg I,Schoenfeld AR

    更新日期:2015-02-22 00:00:00

  • Androgen receptor and gene network: Micromechanics reassemble the signaling machinery of TMPRSS2-ERG positive prostate cancer cells.

    abstract::Prostate cancer is a gland tumor in the male reproductive system. It is a multifaceted and genomically complex disease. Transmembrane protease, serine 2 and v-ets erythroblastosis virus E26 homolog (TMPRSS2-ERG) gene fusions are the common molecular signature of prostate cancer. Although tremendous advances have been ...

    journal_title:Cancer cell international

    pub_type: 杂志文章,评审


    authors: Farooqi AA,Hou MF,Chen CC,Wang CL,Chang HW

    更新日期:2014-04-17 00:00:00

  • Mst1 overexpression combined with Yap knockdown augments thyroid carcinoma apoptosis via promoting MIEF1-related mitochondrial fission and activating the JNK pathway.

    abstract:Background:Cancer cell viability is strongly modulated by the Hippo pathway, which includes mammalian STE20-like protein kinase 1 (Mst1) and yes-associated protein (Yap). Although the roles of Mst1 and Yap in thyroid carcinoma cell death have been fully addressed, no study has determined whether differential modificati...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Zhang X,Li F,Cui Y,Liu S,Sun H

    更新日期:2019-05-22 00:00:00

  • The heterogeneous clinical and pathological landscapes of metastatic Braf-mutated colorectal cancer.

    abstract::Colorectal cancer (CRC) is a complex and molecularly heterogeneous disease representing one of the most frequent causes of cancer-related death worldwide. About 8-15% of CRCs harbor a mutation in BRAF gene, a proto-oncogene involved in cell proliferation, differentiation and survival through the MAPK signaling cascade...

    journal_title:Cancer cell international

    pub_type: 杂志文章,评审


    authors: Fanelli GN,Dal Pozzo CA,Depetris I,Schirripa M,Brignola S,Biason P,Balistreri M,Dal Santo L,Lonardi S,Munari G,Loupakis F,Fassan M

    更新日期:2020-01-29 00:00:00

  • TMPO-AS1 promotes cell proliferation of thyroid cancer via sponging miR-498 to modulate TMPO.

    abstract:Background:Thyroid cancer (TC) is the most frequent endocrine malignancy. Long noncoding RNAs (lncRNAs) have been confirmed to act as significant roles in tumor development. The role of lncRNA TMPO-AS1 in TC is still unclear, so it remains to be explored. The aim of the research is to investigate the role and regulator...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Li Z,Feng Y,Zhang Z,Cao X,Lu X

    更新日期:2020-07-08 00:00:00

  • Prognostic value of CD44 expression in patients with hepatocellular carcinoma: meta-analysis.

    abstract:BACKGROUND:CD44 has been reported to be involved with tumor growth and metastasis and has also been implicated as a CSC marker in hepatocellular carcinoma (HCC). However, the prognostic value of CD44 still remains controversial; hence, we investigated the correlation between CD44 and the clinicopathological features of...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Luo Y,Tan Y

    更新日期:2016-06-17 00:00:00

  • Silencing lncRNA ZFAS1 or elevated microRNA-135a represses proliferation, migration, invasion and resistance to apoptosis of osteosarcoma cells.

    abstract:Background:Osteosarcoma (OS) is still a disease with high mortality from malignant tumors in children and adolescents. Due to its poor treatment, this study explored the involvement of lncRNA ZFAS1/microRNA-135a (miR-135a)/apurinic/apyrimidinic exonuclease 1 (APEX1) axis in the regulation of OS growth and metastasis. ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Zhao Z,Lin X,Tong Y,Li W

    更新日期:2019-12-03 00:00:00

  • RASSF-1A modulates proliferation-mediated oral squamous cell carcinoma progression.

    abstract:Background:To investigate the expression of RASSF-1A in oral squamous cell carcinoma (OSCC) and adjacent tissues, and to explore its mechanism of action in the development of OSCC. Methods:RASSF-1A and proliferation-related protein expression in clinical and OSCC mouse models were detected by qPCR and western blot. In...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Sun J

    更新日期:2019-08-13 00:00:00

  • Bioinformatics analysis combined with experiments to explore potential prognostic factors for pancreatic cancer.

    abstract:Background:Pancreatic cancer is a common malignant tumor of the digestive tract. It has a high degree of malignancy and poor prognosis. Finding effective molecular markers has great significance for pancreatic cancer diagnosis and treatment. This study aimed to investigate DLGAP5 expression in pancreatic cancer and exp...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Ke MJ,Ji LD,Li YX

    更新日期:2020-08-08 00:00:00

  • MicroRNA-32 promotes cell proliferation, migration and suppresses apoptosis in breast cancer cells by targeting FBXW7.

    abstract:BACKGROUND:MicroRNAs are a class of small non-coding RNAs that are involved in many important physiological and pathological processes by regulating gene expression negatively. The purpose of this study was to investigate the effect of miR-32 on cell proliferation, migration and apoptosis and to determine the functiona...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Xia W,Zhou J,Luo H,Liu Y,Peng C,Zheng W,Ma W

    更新日期:2017-01-25 00:00:00

  • SENP1-mediated deSUMOylation of USP28 regulated HIF-1α accumulation and activation during hypoxia response.

    abstract:Background:The ubiquitin-specific protease 28 (USP28) is an oncogenic deubiquitinase, which plays a critical role in tumorigenesis via antagonizing the ubiquitination and degradation of tumor suppressor protein FBXW7-mediated oncogenic substrates. USP28 controls hypoxia-dependent angiogenesis and metastasis by preventi...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Du SC,Zhu L,Wang YX,Liu J,Zhang D,Chen YL,Peng Q,Liu W,Liu B

    更新日期:2019-01-03 00:00:00

  • The prognostic role of the cancer stem cell marker CD44 in ovarian cancer: a meta-analysis.

    abstract:BACKGROUND:CD44 has recently been reported as a cancer stem cell marker in ovarian cancer. However, the clinicopathological and prognostic value of this marker in ovarian cancer remains controversial; Here, we aimed to investigate the correlation between CD44 expression and the clinicopathological features or survival ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Lin J,Ding D

    更新日期:2017-01-05 00:00:00

  • Targeting cellular metabolism using rapamycin and/or doxycycline enhances anti-tumour effects in human glioma cells.

    abstract:Background:Glioma is the most common highly aggressive, primary adult brain tumour. Clinical data show that therapeutic approaches cannot reach the expectations in patients, thus gliomas are mainly incurable diseases. Tumour cells can adapt rapidly to alterations during therapeutic treatments related to their metabolic...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Petővári G,Hujber Z,Krencz I,Dankó T,Nagy N,Tóth F,Raffay R,Mészáros K,Rajnai H,Vetlényi E,Takács-Vellai K,Jeney A,Sebestyén A

    更新日期:2018-12-19 00:00:00

  • Downregulation of long non-coding RNA MAFG-AS1 represses tumorigenesis of colorectal cancer cells through the microRNA-149-3p-dependent inhibition of HOXB8.

    abstract:Background:Colorectal cancer (CRC) is considered as the second common death-induced cancer. More recently, association of long non-coding RNAs (lncRNAs) with CRC has been extensively investigated. Therefore, the present study was performed to determine whether lncRNA MAF BZIP Transcription Factor G Antisense RNA 1 (MAF...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Ruan Z,Deng H,Liang M,Xu Z,Lai M,Ren H,Deng X,Su X

    更新日期:2020-10-19 00:00:00