Abstract：
BACKGROUND:The goal of this study was to identify the nature of the inclusion bodies that have been found in HeLa cells (cervical cancer immortal cell line) by electron microscope and to determine whether the major capsid protein (L1) of human papillomavirus (HPV) can be expressed in HPV-positive uterine cervix cancer cells. METHODS:HPV L1 protein expression in HeLa cells was detected with anti-HPV L1 multivalent mice monoclonal antibody and rabbit polyclonal anti-HPV L1 antibody by ELISA, light microscope immunohistochemistry, electron microscope immunocytochemistry and Western blotting assays. Reverse transcriptional PCR (RT-PCR) was performed to detect the transcription of L1 mRNA in HeLa cells. The immortalized human keratinocyte HeCat was used as the negative control. RESULTS:HPV L1 proteins reacted positively in the lysate of HeLa cells by ELISA assays. HRP labeled light microscope immunohistochemistry assay showed that there was a strong HPV L1 positive reaction in HeLa cells. Under the electron microscope, irregular shaped inclusion bodies, assembled by many small and uniform granules, had been observed in the cytoplasm of some HeLa cells. These granules could be labeled by the colloidal gold carried by HPV L1 antibody. The Western blotting assay showed that there was a L1 reaction strap at 80-85 kDa in the HeLa cell lysates, hence demonstrating the existence of HPV18 L1 in HeLa cells. RT-PCR assay showed that the L1 mRNA was transcribed in HeLa cells. CONCLUSIONS:The inclusion bodies found in the cytoplasm of HeLa cells are composed of HPV18 L1 protein. Since HeLa cell line is a type of cervical cancer cells, this implies that HeLa cells have the ability to express HPV L1 proteins.
journal_name
Cancer Cell Intjournal_title
Cancer cell internationalauthors
Xiao CY,Fu BB,Li ZY,Mushtaq G,Kamal MA,Li JH,Tang GC,Xiao SSdoi
10.1186/s12935-015-0206-0subject
Has Abstractpub_date
2015-05-23 00:00:00pages
53issn
1475-2867pii
206journal_volume
15pub_type
杂志文章abstract：BACKGROUND:LncRNA HOXA-AS2 has been found in the literature to deteriorate glioblastoma. However, its regulatory mechanism is yet to be fully investigated. Our study focused chiefly on the interaction and role of the HOXA-AS2/miR-885-5p/RBBP4 axis in the development of glioblastoma. METHODS:qRT-PCR analysis was perfor...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01690-1
更新日期：2021-01-11 00:00:00
abstract：Background:MicroRNAs have been involved in regulating crucial biological function in some tumors. However, the clinical role and functional effects of miR-591 in breast cancer remain unknown. Methods:The expression of miR-591 was detected in breast cancer tissues and their paired normal tissues by qRT-PCR. Functional ...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-019-0818-x
更新日期：2019-04-24 00:00:00
abstract：Background:In recent years, the B cell receptor (BCR) signaling pathway has become a "hot point" because it plays a critical role in B-cell proliferation and function. Bruton's tyrosine kinase (BTK) is overexpressed in many subtypes of B-cell lymphoma as a downstream kinase in the BCR signaling pathway. Ibrutinib, the ...
journal_title：Cancer cell international
pub_type： 杂志文章,评审
doi：10.1186/s12935-020-01518-y
更新日期：2020-09-29 00:00:00
abstract：BACKGROUND:Abnormal proliferation, metastasis and epithelial-mesenchymal transformation (EMT) of lens epithelial cells (LECs) are direct factors of posterior capsular opacification (PCO). Nuclear enriched abundant transcript 1 (NEAT1) has been shown to promote cell proliferation, metastasis and EMT, but whether it affe...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01619-8
更新日期：2020-10-31 00:00:00
abstract：Background:Lymph node metastasis is one of the most important factors affecting the prognosis of tongue cancer, and the molecular mechanism regulating lymph node metastasis of tongue cancer is poorly known. Methods:The gene expression dataset GSE2280 and The Cancer Genome Atlas (TCGA) tongue cancer dataset were downlo...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-019-1028-2
更新日期：2019-11-21 00:00:00
abstract：BACKGROUND:Natural killer (NK) cells eliminate virus-infected and tumor cells through the release of perforins and granzymes; they also produce Interferon gamma (IFN-γ) and Tumor necrosis factor alpha (TNF-α), which induce apoptosis in target cells. Many tumors express Heme oxygenase 1 (HO-1), and this expression has b...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-014-0100-1
更新日期：2014-10-01 00:00:00
abstract：BACKGROUND:Long non-coding RNAs (lncRNAs) are increasingly implicated in the regulation of the progression of malignancy. AIM:To clarify the relations among BCYRN1 (brain cytoplasmic RNA 1, a long non-coding RNA), c-MYC and cell metastasis of non-small-cell lung cancer (NSCLC). METHODS:Real-time PCR was used to measu...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-015-0183-3
更新日期：2015-04-01 00:00:00
abstract：BACKGROUND:Mutational inactivation of the von Hippel-Lindau (VHL) tumor suppressor gene has been linked to hereditary as well as sporadic clear cell renal carcinomas. The product of the VHL gene, pVHL, acts to target hypoxia-inducible factor alpha (HIF-alpha) subunits for ubiquitination and subsequent degradation. Usin...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/1475-2867-7-13
更新日期：2007-06-28 00:00:00
abstract：Background:Cutaneous melanoma is one of the most aggressive and lethal skin cancers. It is greatly important to identify prognostic biomarkers to guide the clinical management. However, it is technically challenging for untrained researchers to process high dimensional profiling data and identify potential prognostic g...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01262-3
更新日期：2020-05-19 00:00:00
abstract：Background:The staging system of nasopharyngeal carcinoma (NPC) has close relationship with the degree of cell differentiation, but most NPC patients remain undiagnosed until advanced phases. Novel metabolic markers need to be characterized to support diagnose at an early stage. Methods:Metabolic characteristics of na...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-019-0759-4
更新日期：2019-02-18 00:00:00
abstract：Background:Laryngopharyngeal reflux (LPR), with its increasing morbidity, is attracting considerable attention. In recent years, the causal role between LPR and laryngeal carcinoma has been debated. The main harmful component of LPR is pepsin, which has been shown to induce mucosal inflammation by damaging the mucous m...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-019-0772-7
更新日期：2019-03-20 00:00:00
abstract：Background:Emergence of resistance to molecular targeted therapy constitutes a limitation to clinical benefits in cancer treatment. Cross-resistance commonly happens with chemotherapeutic agents but might not with targeted agents. Methods:In the current study, TP53 wild-type cell lines with druggable MAPK pathway muta...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-019-0768-3
更新日期：2019-03-07 00:00:00
abstract：:N6-methyl-adenosine(m6A) modification emerges as an abundant and dynamic regulation throughout the Eukaryotic transcriptome. Dysregulation of the m6A regulators has increasingly been found in many neoplasms. It is reasonable to believe that m6A changes the fate of cancer cells and subsequently affected all aspects of ...
journal_title：Cancer cell international
pub_type： 杂志文章,评审
doi：10.1186/s12935-020-01445-y
更新日期：2020-07-29 00:00:00
abstract：BACKGROUND:CD44 has recently been reported as a cancer stem cell marker in ovarian cancer. However, the clinicopathological and prognostic value of this marker in ovarian cancer remains controversial; Here, we aimed to investigate the correlation between CD44 expression and the clinicopathological features or survival ...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-016-0376-4
更新日期：2017-01-05 00:00:00
abstract：BACKGROUND:miR-222 has been reported to be overexpressed in colorectal cancer and it influences cancer cell proliferation, drug resistance and metastasis. However, the underlying molecular mechanism of miR-222 in colorectal cancer cell invasion and migration has not been thoroughly elucidated to date. METHODS:The cell...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-017-0447-1
更新日期：2017-08-29 00:00:00
abstract：Background:Nuclear transcription factor kappa B (NF-κB) subunits exhibit crucial roles in tumorigenesis and chemo-sensitivity. Recent studies suggest that RelB, the key subunit of the alternative NF-κB pathway, plays a critical role in the progression of diverse human malignancies. However, the significance of RelB in ...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-018-0677-x
更新日期：2018-11-13 00:00:00
abstract：:Cancer is a major public health problem as one of the leading causes of death worldwide. Deciphering the molecular regulation mechanisms of tumor progression can make way for tumor diagnosis and therapy. Paternally expressed gene 10 (PEG10), located on human chromosome 7q21.3, has turned out to be an oncogene implicat...
journal_title：Cancer cell international
pub_type： 杂志文章,评审
doi：10.1186/s12935-018-0610-3
更新日期：2018-08-13 00:00:00
abstract：Background:Thyroid cancer is the most common type of endocrine malignancy and the incidence rate is rapidly increasing worldwide. Epigallocatechin-3-gallate (EGCG) could suppress cancer growth and induce apoptosis in many types of cancer cells. However, the mechanism of action of EGCG on the growth of human thyroid car...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-019-0762-9
更新日期：2019-02-28 00:00:00
abstract：UNLABELLED: BACKGROUND:Anticancer research resulted in the discovery of a promising antimitotic metabolite, 2-methoxyestradiol. 2-Methoxyestradiol-bis-sulphamate, a bis-sulphamoylated analogue exerts antiproliferative- and antimitotic activity. Investigating the anticancer potential of 2-methoxyestradiol-bis-sulphamat...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/1475-2867-12-37
更新日期：2012-08-20 00:00:00
abstract：:An area of research that has been recently gaining attention is the relationship between cancer stem cell (CSC) biology and chemo-resistance in colon cancer patients. It is well recognized that tumor initiation, growth, invasion and metastasis are promoted by CSCs. An important reason for the widespread interest in th...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-015-0163-7
更新日期：2015-01-28 00:00:00
abstract：BACKGROUND:Prostate cancer is a leading cause of male cancer specific mortality. When cure by radical prostatectomy is not possible the next line of prostate cancer treatment is androgen deprivation. However prolonged androgen deprivation often results in relapse and androgen-independent prostate cancer that is inevita...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/1475-2867-8-3
更新日期：2008-03-18 00:00:00
abstract：Background:Melanoma as a type of skin cancer, is associated with a high mortality rate. Therefore, early diagnosis and efficient surgical treatment of this disease is very important. Photodynamic therapy (PDT) involves the activation of a photosensitizer by light at specific wavelength that interacts with oxygen and cr...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01616-x
更新日期：2020-10-29 00:00:00
abstract：:Sineoculis homeobox homolog 1 (Six1), normally a developmentally restricted transcriptional regulator, is frequently dysregulated in mutiple cancers. Increasing evidences show that overexpression of Six1 plays a key role in tumorigenesis. However, the Six1 expression status and its relationship with the clinicopatholo...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-015-0215-z
更新日期：2015-06-20 00:00:00
abstract：:[This retracts the article DOI: 10.1186/s12935-015-0237-6.]. ...
journal_title：Cancer cell international
pub_type： 撤回出版物
doi：10.1186/s12935-016-0359-5
更新日期：2016-11-04 00:00:00
abstract：:Non-small-cell lung cancer (NSCLC) is a type of malignancy with progressive metastasis having poor prognosis and lowered survival resulting from late diagnosis. The therapeutic approaches for the treatment of this incurable cancer are chemo- and radiotherapy. Since current treatments are insufficient and because of dr...
journal_title：Cancer cell international
pub_type： 杂志文章,评审
doi：10.1186/s12935-019-0853-7
更新日期：2019-05-16 00:00:00
abstract：BACKGROUND:Blockade of the angiotensin (ANG) II type 1 receptor (AT1R) inhibits tumour growth in several cancers, including colorectal cancer (CRC) liver metastases. While AT1R blockade has been extensively studied, the potential of targeting the antagonistically acting AT2R in cancer has not been investigated. This st...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/1475-2867-10-19
更新日期：2010-06-28 00:00:00
abstract：BACKGROUND:The thieno[2,3-b]pyridines were discovered by virtual high throughput screening as potential inhibitors of phospholipase C (PLC) isoforms and showed potent growth inhibitory effects in National Cancer Institute's human tumour cell line panel (NCI60). The mechanism of the anti-proliferative activity of thieno...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-016-0293-6
更新日期：2016-03-10 00:00:00
abstract：BACKGROUND:CircRNAs play crucial roles in multiple tumours. However, the functions of most circRNAs in cervical cancer remain unclear. METHODS:This study collected GSE113696 data from the GEO database to search for differentially expressed circRNAs in cervical cancer. Quantitative reverse transcription PCR was used to...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01736-4
更新日期：2021-01-07 00:00:00
abstract：BACKGROUND:Mononuclear platinum anticancer agents hold a pivotal place in the treatment of many forms of cancers, however, there is a potential to improve response to evade resistance development and toxic side effects. BBR3464 is a promising trinuclear platinum anticancer agent, which is a polyamine mimic. The aim was...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/1475-2867-12-20
更新日期：2012-05-28 00:00:00
abstract：Background:Chimeric antigen receptors (CARs) represent a novel facet of modern day synthetic biology that exemplifies personalized medicine at work through their ability to harness and redirect a patient's immune system to fight cancer. Body:By combining the target-specificity of antibodies to the effector capabilitie...
journal_title：Cancer cell international
pub_type： 杂志文章,评审
doi：10.1186/s12935-018-0685-x
更新日期：2018-11-14 00:00:00
