Abstract:
Background:Neuroblastoma (NB) is the most common malignant tumor originating from the extracranial sympathetic nervous system in children. The molecular mechanisms underlying this disease are complex, and not completely understood. Methods:Quantitative real-time PCR (qRT-PCR) was applied to quantify the expression of miR-20a-5p and its target gene ATG7 in clinical NB tissues. The biological function of miR-20a-5p and ATG7 in SH-SY5Y cells was investigated through in vitro studies (Real-Time cell kinetic analyzer, colony formation assay, caspase-Glo 3/7 assay and western blotting). The luciferase reporter assay was conducted to verify the biological relationship between miR-20a-5p and ATG7. Results:Here we found that miR-20a-5p expression was significantly downregulated whereas its target autophagy-related gene 7 (ATG7) was increased along with clinical staging of NB progression. Correlation analysis showed that miR-20a-5p had a negative correlation trend with ATG7. In SH-SY5Y cells, forced expression of miR-20a-5p suppressed ATG7 expression, autophagy initiation and cellular proliferation while promoted apoptosis, suggesting a potential association between miR-20a-5p and ATG7. Further bioinformatic target prediction combined with protein expression and luciferase reporter assay verified that miR-20a-5p inhibited ATG7 by directly binding to its 3'-UTR, confirming the involvement of miR-20a-5p in the regulation of ATG7 in NB. Conclusions:These results clarified that miR-20a-5p inhibited cell proliferation and promoted apoptosis through negative regulation of ATG7 and thus autophagy suppression in SH-SY5Y cells. Therefore, defining the context-specific roles of autophagy in NB and regulatory mechanisms involved will be critical for developing autophagy-targeted therapeutics against NB. Both miR-20a-5p and ATG7 would be potential therapeutic targets for future NB treatment.
journal_name
Cancer Cell Intjournal_title
Cancer cell internationalauthors
Yu Y,Zhang J,Jin Y,Yang Y,Shi J,Chen F,Han S,Chu P,Lu J,Wang H,Guo Y,Ni Xdoi
10.1186/s12935-017-0499-2subject
Has Abstractpub_date
2018-01-04 00:00:00pages
5issn
1475-2867pii
499journal_volume
18pub_type
杂志文章abstract:BACKGROUND:Avenanthramides (AVN) are a relatively unstudied family of phytochemicals that could be novel chemotherapeutics. These compounds, found in oats, are non-toxic to healthy cells and have been shown to reduce viability of human colon and liver cancers in vitro. However, these studies do not elucidate a molecula...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-017-0464-0
更新日期:2017-10-18 00:00:00
abstract:OBJECTIVES:Bladder transitional cell carcinoma (TCC) is one of the most common solid malignancies in China. This study examined the antitumor effect and underlying mechanism of action of sunitinib malate in human bladder TCC in vitro. METHODS:Bladder TCC cell lines 5637 and BIU87 were maintained in 1640 medium and T24...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-015-0179-z
更新日期:2015-02-28 00:00:00
abstract:Background:Endometrial carcinoma (EC) is one of the three major malignant tumors of the female reproductive system. In recent years, the incidence and mortality rate of EC have increased. B-cell translocation gene 1 (BTG1) is an anti-proliferation gene that regulates the occurrence and development of a variety of tumor...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01591-3
更新日期:2020-10-07 00:00:00
abstract:Background:Long noncoding RNAs (lncRNAs) play essential roles in tumor progression. However, the functions and targets of lncRNAs in neuroblastoma (NB) progression still remain to be determined. In this study, we aimed to investigate the effect of lncRNA DLX6 antisense RNA 1 (DLX6-AS1) on NB and the underlying mechanis...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-0968-x
更新日期:2019-11-27 00:00:00
abstract:BACKGROUND:CircRNAs play crucial roles in multiple tumours. However, the functions of most circRNAs in cervical cancer remain unclear. METHODS:This study collected GSE113696 data from the GEO database to search for differentially expressed circRNAs in cervical cancer. Quantitative reverse transcription PCR was used to...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01736-4
更新日期:2021-01-07 00:00:00
abstract:BACKGROUND:Bladder cancer (BCa) is a malignant tumor that occurs on the mucosa of the bladder, in which dysregulated long non-coding RNAs (lncRNAs) are involved. This study investigated the effect of lncRNA small nucleolar RNA host gene 1 (SNHG14) on the biological characteristics of BCa cells from microRNA (miR)-211-3...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01717-7
更新日期:2021-01-22 00:00:00
abstract:Background:Nuclear transcription factor kappa B (NF-κB) subunits exhibit crucial roles in tumorigenesis and chemo-sensitivity. Recent studies suggest that RelB, the key subunit of the alternative NF-κB pathway, plays a critical role in the progression of diverse human malignancies. However, the significance of RelB in ...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-018-0677-x
更新日期:2018-11-13 00:00:00
abstract:Background:Acute lymphoblastic leukemia (ALL) is an aggressive hematopoietic malignancy that is most commonly observed in children. Alantolactone (ALT) has been reported to exhibit anti-tumor activity in different types of cancer. The aim of the present study was to investigate the anti-tumor activity and molecular mec...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01537-9
更新日期:2020-09-09 00:00:00
abstract:: Autoimmune phenomena were identified in many different cases of hematological diseases and solid tumors, which may be due to alterations in the expression and function of the NF-κB signaling pathway. Recently, a number of studies have shown that the deletion or mutation of A20, a negative regulator of NF-κB, is frequ...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-12-44
更新日期:2012-11-07 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-018-0545-8
更新日期:2018-04-02 00:00:00
abstract:BACKGROUND:Urokinase-type Plasminogen Activator (uPA), a serine protease, plays a pivotal role in human breast cancer metastasis by mediating the degradation of extracellular matrix proteins and promoting cell motility. In more advanced breast cancers, uPA activity is significantly up regulated and serves as a prognost...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-6-16
更新日期:2006-05-31 00:00:00
abstract:BACKGROUND:Compounds of plant origin and food components have attracted scientific attention for use as agents for cancer prevention and treatment. Wine contains polyphenols that were shown to have anti-cancer and other health benefits. The survival pathways of Akt and extracellular signal-regulated kinase (Erk), and t...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-14-6
更新日期:2014-01-23 00:00:00
abstract:BACKGROUND:Gastric cancer is one of the common cancers seen in south India. Unfortunately more than 90% are advanced by the time they report to a tertiary centre in the country. There is an urgent need to characterize these cancers and try to identify potential biomarkers and novel therapeutic targets. MATERIALS AND M...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-10-45
更新日期:2010-11-24 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-0860-8
更新日期:2019-05-22 00:00:00
abstract::[This corrects the article DOI: 10.1186/s12935-019-0742-0.]. ...
journal_title:Cancer cell international
pub_type: 杂志文章,已发布勘误
doi:10.1186/s12935-019-0797-y
更新日期:2019-04-11 00:00:00
abstract:BACKGROUND:Recent studies have reported the involvement of microRNA-29 (miR-29) family members in human cancers through their ability to regulate cellular functions. The present study investigated biological function of miR-29b in colorectal cancer (CRC). METHODS:CRC tissues and adjacent normal tissues were collected ...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01700-2
更新日期:2021-01-06 00:00:00
abstract:Background:Angiogenesis plays critical roles in the progression and metastasis of malignant tumors. Gastric neuroendocrine carcinoma is an uncommon stomach cancer that is rich in blood vessels and exhibits highly malignant biological behavior with a poor prognosis. The role of CDK5RAP3 in GNEC has not been reported to ...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-0997-5
更新日期:2019-11-07 00:00:00
abstract:Background:Abnormal expressions of long noncoding RNAs (lncRNAs) are very common in clear cell renal cell carcinoma (ccRCC), and some of these have been reported to be highly correlated with prognosis of ccRCC patients. Methods:"edgeR" AND "DEseq" R packages were used to explore differentially expressed genes (DEGs) b...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-0928-5
更新日期:2019-08-05 00:00:00
abstract:Background:Lymph node metastasis is one of the most important factors affecting the prognosis of tongue cancer, and the molecular mechanism regulating lymph node metastasis of tongue cancer is poorly known. Methods:The gene expression dataset GSE2280 and The Cancer Genome Atlas (TCGA) tongue cancer dataset were downlo...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-019-1028-2
更新日期:2019-11-21 00:00:00
abstract:BACKGROUND:Chemoresistance is a major obstacle to successful chemotherapy for colorectal cancer. Eukaryotic translation initiation factor 5A2 (eIF5A2), one of the two isoforms in the eIF5A family, has been reported to be a new oncogene in many types of human cancer. In the present study, we aimed to investigate whether...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-015-0250-9
更新日期:2015-11-17 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-11-35
更新日期:2011-10-28 00:00:00
abstract:BACKGROUND:Although recent studies have shown the utility of miR-203 as a cancer-relevant biomarker, the validated clinical significance of miR-203 in HCC remains obscure. The aim of the present study was to evaluate the relationship between miR-203 expression and clinicopathological features in HCC patients. METHODS:...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-015-0214-0
更新日期:2015-06-18 00:00:00
abstract:Background:Cancer-associated fibroblasts (CAFs) play important roles in tumor progression. However, the behaviors of activated CAFs in gastric cancer remain to be determined. The aim of the present study was to investigate the correlations between activated gastric CAFs and the prognosis of patients with gastric cancer...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-018-0599-7
更新日期:2018-07-20 00:00:00
abstract:Background:Increasing evidences have underlined the importance of long non-coding RNAs (lncRNAs) in human malignancies. LINC00958 has been found involved in some cancers. However, the underlying mechanical performance of LINC00958 in lung adenocarcinoma (LAD) has not been explored yet. Methods:The expression of releva...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-1099-0
更新日期:2020-01-23 00:00:00
abstract:BACKGROUND:While some risk factors for breast cancer are well-known, the influence of other factors, particularly virus infection, remains unclear. Human cytomegalovirus (HCMV) is widespread in the general population, and both molecular and epidemiological evidence has indicated links between HCMV and breast cancer. Th...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-017-0399-5
更新日期:2017-02-13 00:00:00
abstract:BACKGROUND:Non-small cell lung cancer (NSCLC) is the largest histological subgroup of lung cancer and has increased in prevalence in China over the past 5 years. The 5-year survival rate has remained at 15-20 %, with a median survival of 8-12 months. The tumorigenesis and progression of NSCLC is orchestrated by numerou...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-015-0236-7
更新日期:2015-09-17 00:00:00
abstract:BACKGROUND:Cancer cells, even in the presence of available oxygen, have a glycolysis enhancement. The "aerobic glycolysis" is known as the Warburg effect and it is considered one of the fundamental hallmarks of metabolic alteration during malignant transformation. A feature of many tumors is also a change into ions equ...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-014-0077-9
更新日期:2014-08-12 00:00:00
abstract::Circular RNAs (circRNAs) are small non-coding RNAs with a unique ring structure and play important roles as gene regulators. Disturbed expressions of circRNAs is closely related to varieties of pathological processes. The roles of circRNAs in cancers have gained increasing concerns. The communications between the canc...
journal_title:Cancer cell international
pub_type: 杂志文章,评审
doi:10.1186/s12935-020-01301-z
更新日期:2020-06-03 00:00:00
abstract:Background:Dysregulation of long non-coding RNAs (lncRNAs) results in development of human diseases including hepatocellular carcinoma (HCC). Although several HCC related lncRNAs have been reported, the biological functions of many lncRNAs during the development of HCC remains unknown. Methods:The expression of ST8SIA...
journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-01325-5
更新日期:2020-06-11 00:00:00
abstract::Colorectal cancer (CRC) is a complex and molecularly heterogeneous disease representing one of the most frequent causes of cancer-related death worldwide. About 8-15% of CRCs harbor a mutation in BRAF gene, a proto-oncogene involved in cell proliferation, differentiation and survival through the MAPK signaling cascade...
journal_title:Cancer cell international
pub_type: 杂志文章,评审
doi:10.1186/s12935-020-1117-2
更新日期:2020-01-29 00:00:00