Capture EIA for determination of IgE to diphtheria and tetanus toxins in comparison to RIA.

Abstract:

:A capture IgE assay in EIA with diphtheria toxin and tetanus toxoid as model systems was developed and evaluated in relation to RIA in a DT booster study. The EIA and the RIA gave very similar results, although the EIA determined IgE over a wider range of concentrations, without interference by other subclasses. The comparison to the capture EIA indicated that the RIA gave more reliable results when a toxin as antigen. Weak correlations between local side effects and IgE responses and differences between vaccine groups were noted by RIA but not by EIA, which correlated better with the clinical findings.

journal_name

Vaccine

journal_title

Vaccine

authors

Wahren E,Bengtsson C,Björkstén B,Mark A,Granström M

doi

10.1016/s0264-410x(97)00274-0

subject

Has Abstract

pub_date

1998-05-01 00:00:00

pages

873-6

issue

9-10

eissn

0264-410X

issn

1873-2518

pii

S0264-410X(97)00274-0

journal_volume

16

pub_type

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