Abstract：
:Bovine insulin was glycated by in vitro incubation with 20-220 mM D-glucose for 1-48 h. The percentage of glycation was dependent on time, glucose concentration, temperature and pH, attaining values up to 28%. Glucose-lowering activities of glycated and control (non-glycated) insulin preparations were assessed in mice by intraperitoneal injection in a 39% (w/v) glucose solution (2 g/kg body weight) at doses of 0.05 and 0.25 units/kg body weight. Injection of glucose alone significantly (P < 0.001) increased plasma glucose concentrations at 30 min. Simultaneous administration of non-glycated insulin with glucose significantly decreased the 30-min glycaemic excursion (P < 0.001) in a dose-dependent manner. Glycated insulin exhibited a significant reduction (P < 0.001) in glucose-lowering activity under these conditions. The relationship between the extent of insulin glycation and glucose-lowering activity at 0.25 units/kg was assessed using five different insulin preparations glycated between 6%-28%. The insulin-induced decrease in plasma glucose at 30 min was inversely related to the extent of glycation (r = 0.99). Glycated insulin (10(-8) and 10(-6) M) also exhibited a significantly reduced (P < 0.05) ability to stimulate glucose oxidation in isolated mouse diaphragm muscle compared with non-glycated insulin. These data indicate that glycated insulin exhibits impaired biological activity which may contribute to glucose intolerance in diabetes. Further studies are required to determine if glycation of insulin occurs in man and if this process contributes to the pathogenesis of diabetes.
journal_name
Acta Diabetoljournal_title
Acta diabetologicaauthors
Abdel-Wahab YH,O'Harte FP,Boyd AC,Barnett CR,Flatt PRdoi
10.1007/s005920050086subject
Has Abstractpub_date
1997-12-01 00:00:00pages
265-70issue
4eissn
0940-5429issn
1432-5233journal_volume
34pub_type
杂志文章abstract：:The objective is to assess the effect of TNF-alpha inhibition on urinary albumin excretion in experimental diabetic rats. Male Wistar rats, 8-week-old, were categorized into four groups, which were the control (n = 9), diabetes (n = 9), infliximab-treated diabetes (n = 10), and FR167653-treated diabetes (n = 9) groups...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-007-0007-6
更新日期：2007-12-01 00:00:00
abstract：:The purification of large numbers of human pancreatic islets remains one of the limiting factors in islet transplantation. This paper describes and validates a method for accurately and reproducibly determining the density of islets and exocrine tissue in pancreatic digest on the basis of their isopycnic distribution ...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/BF00572864
更新日期：1993-01-01 00:00:00
abstract：AIMS:Diabetic retinopathy (DR) is characterized by early dropout of capillary pericytes, leading to loss of control on endothelial proliferation and, subsequently, angiogenesis. We have demonstrated that extracellular vesicles (EV) derived from mesenchymal stem cells (MSC) maintained in diabetic-like conditions may pla...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-015-0798-9
更新日期：2015-12-01 00:00:00
abstract：AIMS:This subanalysis of a cross-sectional, nationwide study was undertaken to assess the relationship between healthy lifestyle behaviors and multiple cardiovascular risk factors among people with type 2 diabetes mellitus (T2DM). METHODS:Data collected from 25,454 participants, including demographics, lifestyle behav...
journal_title：Acta diabetologica
pub_type： 杂志文章,多中心研究
doi：10.1007/s00592-017-0981-2
更新日期：2017-06-01 00:00:00
abstract：:Our aim was to compare the effects of an intermediate acting human insulin (NPH) and a long-acting insulin analog, insulin glargine, in insulin naïve type 2 diabetes patients, stratified by the type of hyperglycemia (fasting or postprandial type). Based on different action profiles, we hypothesized that patients havin...
journal_title：Acta diabetologica
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00592-013-0505-7
更新日期：2014-04-01 00:00:00
abstract：AIMS:Medication adherence and persistence are important determinants of treatment success in type 2 diabetes mellitus (T2DM). This systematic review and meta-analysis evaluated the real-world adherence, persistence, and in-class switching among patients with T2DM prescribed dipeptidyl peptidase-4 (DPP4) inhibitors. ME...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-020-01590-w
更新日期：2021-01-01 00:00:00
abstract：:The title "Plasma fatty acid-binding protein 4 (FABP4) as a novel biomarker to predict gestational diabetes mellitus" should be replaced by "Serum fatty acid-binding protein 4 (FABP4) as a novel biomarker to predict gestational diabetes mellitus". ...
journal_title：Acta diabetologica
pub_type： 已发布勘误
doi：10.1007/s00592-018-1177-0
更新日期：2018-10-01 00:00:00
abstract：:The purpose of this study was to determine the feasibility of a flexible multiple daily insulin (FMDI) regimen in routine pediatric diabetes care by comparing HbA(1c), body mass index (BMI), and episodes of severe hypoglycemia (SH) before and after initiation of FMDI therapy. Data from 44 patients (2-16 years old), on...
journal_title：Acta diabetologica
pub_type： 临床试验,杂志文章
doi：10.1007/s00592-003-0102-2
更新日期：2003-09-01 00:00:00
abstract：AIMS:The present study aims to investigate the association between serum ferritin and diabetes, diabetes control, and insulin resistance (IR) and examine whether gender is a modifier for these associations in a community-based sample. METHODS:A cross-sectional survey of 8,235 participants was conducted in 2009. Serum ...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-014-0656-1
更新日期：2014-12-01 00:00:00
abstract：AIMS:Type 2 diabetes (DM2) is associated to oxidative modifications of high-density lipoproteins (HDL), which can interfere with their function. Pioglitazone has proved effective in raising HDL cholesterol (HDL-C) and lowering small dense low-density lipoprotein (LDL), but no clinical studies have examined its effect o...
journal_title：Acta diabetologica
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00592-018-01278-2
更新日期：2019-05-01 00:00:00
abstract：:Diabetes mellitus, the most common serious metabolic disorder, is characterized by functional and structural changes in the peripheral and central nervous systems. Glial cells provide structural and metabolic support for retinal neurons. During diabetes, one of the early pathogenic events is retinal glial reactivity. ...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-004-0155-x
更新日期：2004-09-01 00:00:00
abstract：:The aim of this study was to assess the effectiveness and safety of intracavernous injections of a four-drug vasoactive mixture in diabetic patients with organic impotence. A group of 60 diabetic patients with either pure neurogenic, pure vasculogenic or mixed neurovasculogenic impotence were treated with intracaverno...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/BF00580752
更新日期：1994-04-01 00:00:00
abstract：:Selective loss of capillary pericytes occurs early and specifically in diabetic retinopathy. We have investigated whether blood derivatives from patients with long-term type 1 (insulin-dependent) diabetes and no retinopathy differ from those with retinopathy and/or non-diabetic controls in their ability to stimulate D...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/BF00572854
更新日期：1993-01-01 00:00:00
abstract：:Insulin resistance is a major factor in the pathogenesis of type 2 diabetes mellitus (T2DM) and is related to the fatty acid profile of the plasma membranes. The purpose of the present study was to investigate fatty acid composition and cholesterol content of cell membranes in patients with type 2 diabetes and, thus, ...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-007-0224-4
更新日期：2006-12-01 00:00:00
abstract：:To study the immune response to glutamic acid decarboxylase (GAD) in insulin-dependent diabetes mellitus, monoclonal GAD antibodies after fusion of splenocytes from a nondiabetes-susceptible BALB/c mouse immunized with human recombinant GAD65 were generated. Of the 44 monoclonals, 35 are specific for the GAD65 isoform...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/BF02048548
更新日期：1996-09-01 00:00:00
abstract：:Kidney volume was measured during pregnancy in insulin-dependent diabetic women by an ultrasound technique and prognostic value of these measurements evaluated. A prospective study was performed on 87 pregnant women with insulin-dependent diabetes attending the maternity clinic of Aarhus Kommunehospital. Patients with...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/BF00576254
更新日期：1995-12-01 00:00:00
abstract：:The aim of the study is to evaluate the prevalence and incidence of myocardial dysfunction (MD) and heart failure (HF) in long-lasting (≥10 years) type 1 diabetes without cardiovascular disorders or with hypertension or coronary heart disease (CHD). The study included 1,685 patients with type 1 diabetes (mean baseline...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-013-0455-0
更新日期：2013-08-01 00:00:00
abstract：:The proinflammatory lysophosphatidic acid (LPA) is a potent activator of several transcriptional factors and signaling pathways and a potent modulator of genes involved in inflammation, angiogenesis and fibrosis. This study was conducted to measure the levels of LPA and LPA-producing enzymes, autotaxin (ATX) and acylg...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-012-0422-1
更新日期：2013-06-01 00:00:00
abstract：:Rosiglitazone often results in weight gain. We hypothesized that rosiglitazone may modulate circulating levels of ghrelin and peptide YY(3-36) and this modulation may be related to weight-gaining effect of this agent. This study was designed as an open-label, randomized, controlled trial of 3-month duration. Women wit...
journal_title：Acta diabetologica
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00592-011-0352-3
更新日期：2012-12-01 00:00:00
abstract：AIMS:Familial partial lipodystrophy (FPLD) is a rare autosomal dominant disorder, mostly due to mutations in lamin A (LMNA) or in peroxisome proliferator-activated receptor gamma (PPARG) genes. In the present study, we aimed to identify and functionally characterize the genetic defect underlying FPLD in an Italian fami...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-019-01462-y
更新日期：2020-05-01 00:00:00
abstract：:We investigated the effect of sub-chronic soybean oil (SO) treatment on the insulin secretion and fatty acid composition of islets of Langerhans obtained from Goto-Kakizaki (GK), a model of type 2 diabetes, and normal Wistar rats. We observed that soybean-treated Wistar rats present insulin resistance and defective is...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-007-0252-8
更新日期：2007-09-01 00:00:00
abstract：:Recent studies have reported that elevated proinsulin levels are indicative of an increased cardiovascular risk. Renal proximal tubular cells represent a major site for the metabolism of insulin-like hormones after glomerular filtration into the tubular lumen. To determine the binding and degradation of proinsulin in ...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/BF00569428
更新日期：1996-07-01 00:00:00
abstract：BACKGROUND:Emerging adulthood is a challenging period for diabetes management. Our aim was to determine whether a dedicated transition clinic for emerging adults with type 1 diabetes can improve glycemic control and visit attendance. METHODS:An observational study of 53 emerging adults (30 males) treated during 2010-2...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-015-0734-z
更新日期：2016-02-01 00:00:00
abstract：:Few studies were performed to evaluate the prevalence of latent autoimmune diabetes in adults (LADA) and the difference of chronic complications between LADA, T1DM, and T2DM in Korean. The aim of this study is to establish the prevalence of LADA in a diabetic clinic of Soonchunhyang University hospital and to compare ...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-010-0228-y
更新日期：2013-04-01 00:00:00
abstract：:Comparing macrophage-derived cytokine and nitric oxide (NO) profiles in type I and type II diabetes mellitus (DM); and determining whether thymoquinone (TQ) has any modulatory effect were the main objectives of the present study. Peritoneal macrophages have been collected from Otsuka Long-Evans Tokushima Fatty (OLETF)...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-005-0170-6
更新日期：2005-03-01 00:00:00
abstract：:Serum gamma-glutamyl transferase (gamma-GT), a marker of oxidative stress, predicts morbidity and mortality from cardiovascular disease. Plasma total homocysteine (tHcy), a pro-oxidant and also an independent risk factor for cardiovascular disease, correlates with gamma-GT among some populations. It is not known wheth...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-007-0001-z
更新日期：2007-12-01 00:00:00
abstract：AIMS:To investigate the associations of vitamin B12 (cobalamin and holotranscobalamin) status with depression, cognition and neuropathy in patients with type 2 diabetes using metformin. METHODS:In an observational study, among 550 type 2 diabetes patients using metformin, cobalamin and holotranscobalamin (holoTCII) le...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-014-0661-4
更新日期：2015-04-01 00:00:00
abstract：:We hypothesize that type 2 diabetic patients with different phenotypes may show different response to incretin-based therapies. Therefore, we tested whether the presence of metabolic syndrome (MS) influences glycemic response to these drugs. We prospectively followed 211 patients, treated with the GLP-1 analog exenati...
journal_title：Acta diabetologica
pub_type： 临床试验,杂志文章
doi：10.1007/s00592-011-0296-7
更新日期：2011-09-01 00:00:00
abstract：AIM:Infection of a diabetic foot ulcer (DFU) is common. More than the half of DFUs become infected and 15-20% of them necessitate amputation in course of treatment. Diabetic foot infection (DFI) is therefore the major cause for non-traumatic lower limb amputation in Germany. Prompt and effective treatment of DFI is man...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-020-01587-5
更新日期：2020-09-17 00:00:00
abstract：:Despite advances in technology, the frequent self-measurement of blood glucose (SMBG) remains fundamental to the management of 1 diabetes mellitus (T1DM). Once measured, SMBG results are routinely reported back to health professionals and other interested parties, either verbally, via a logbook, or electronically down...
journal_title：Acta diabetologica
pub_type： 杂志文章,评审
doi：10.1007/s00592-016-0907-4
更新日期：2017-01-01 00:00:00
