Abstract:
:Recent studies have reported that elevated proinsulin levels are indicative of an increased cardiovascular risk. Renal proximal tubular cells represent a major site for the metabolism of insulin-like hormones after glomerular filtration into the tubular lumen. To determine the binding and degradation of proinsulin in comparison with insulin and insulin-like growth factor-1 (IGF-1), we have used a rabbit proximal tubular cell line (PT-1). As confirmed by electron microscopy. PT-1 cells exhibit bipolar differentiation, demonstrating apical microvilli and invaginations of the basolateral membrane. To allow selective incubation of both compartments, cells were grown on filter membranes. Performing equilibrium binding assays with 125I-labelled hormones, severalfold higher binding was found at the apical than at the basolateral cell membrane, with the capacity range IGF-1 > insulin > proinsulin. Half-maximal displacement of 125I-labelled insulin and IGF-1 was observed at 0.6 and 2 nM, respectively, while crossover binding to the alternate receptor occurred with a 10- to 100-fold lower affinity. Half-maximal displacement of 125I-proinsulin binding was obtained at approx. 8 nM proinsulin and insulin, whereas IGF-1 was 10-fold less potent. The relative degradation of specifically bound tracer was lowest for proinsulin (apical 10%, basolateral: 13%). IGF-1 was degraded by 20% at the apical cell membrane, and up to 78% at the basolateral membrane. In contrast, almost the total amount of insulin bound was degraded at both membrane sites (apical 99%, basolateral: 83%). These results suggest separate insulin and IGF-1 receptors while proinsulin binds with high affinity to a third insulin-like receptor on the apical membrane of PT-1 cells.
journal_name
Acta Diabetoljournal_title
Acta diabetologicaauthors
Jehle PM,Fussgänger RD,Stracke S,Grunewald RW,Keller Fdoi
10.1007/BF00569428subject
Has Abstractpub_date
1996-07-01 00:00:00pages
159-65issue
2eissn
0940-5429issn
1432-5233journal_volume
33pub_type
杂志文章abstract::Obesity, insulin resistance (IR), and progressive decline in pancreatic β-cell function are major features of type 2 diabetes mellitus (T2DM). Altered adipokines contribute to obesity-induced IR. Hence understanding of adipokines' relation to obesity and glycemic control could be useful to improve disease outcomes. We...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-012-0373-6
更新日期:2013-10-01 00:00:00
abstract::Comparing macrophage-derived cytokine and nitric oxide (NO) profiles in type I and type II diabetes mellitus (DM); and determining whether thymoquinone (TQ) has any modulatory effect were the main objectives of the present study. Peritoneal macrophages have been collected from Otsuka Long-Evans Tokushima Fatty (OLETF)...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-005-0170-6
更新日期:2005-03-01 00:00:00
abstract::Diabetic neuropathy is a significant complication of chronic diabetes. We wished to discover whether we were successfully identifying patients who had developed neuropathic problems. Specifically, Diabetologists are traditionally poor at asking about erectile dysfunction (ED) and patients themselves are not always for...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-008-0084-1
更新日期:2009-12-01 00:00:00
abstract::(31)P-magnetic resonance spectroscopy ((31)P-MRS) is a non-invasive tool to study high-energy phosphate (HEP) metabolism. We evaluate whether 31P-MRS can detect early changes in kidney HEP metabolism during a 6-month trial with Valsartan. Twenty consecutive stable and normotensive kidney-transplanted patients were enr...
journal_title:Acta diabetologica
pub_type: 临床试验,杂志文章
doi:10.1007/s00592-012-0369-2
更新日期:2012-12-01 00:00:00
abstract:AIMS:In patients with type 2 diabetes, non-alcoholic fatty liver disease (NAFLD) and liver fibrosis is frequent and presumably associated with increased cardiovascular disease risk and mortality. The objective was to investigate risk factors associated with hepatic fibrosis in patients with type 2 diabetes and NAFLD to...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-019-01374-x
更新日期:2019-11-01 00:00:00
abstract::Considering that changes in exercise routines might have relevance in treatment of adolescents with type 1 diabetes mellitus, we sought to assess whether spontaneous modifications to weekly exercise habits might occur in these patients and whether such variations would be accompanied by alterations in autonomic profil...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-012-0416-z
更新日期:2013-06-01 00:00:00
abstract::Few studies were performed to evaluate the prevalence of latent autoimmune diabetes in adults (LADA) and the difference of chronic complications between LADA, T1DM, and T2DM in Korean. The aim of this study is to establish the prevalence of LADA in a diabetic clinic of Soonchunhyang University hospital and to compare ...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-010-0228-y
更新日期:2013-04-01 00:00:00
abstract:BACKGROUND:Emerging adulthood is a challenging period for diabetes management. Our aim was to determine whether a dedicated transition clinic for emerging adults with type 1 diabetes can improve glycemic control and visit attendance. METHODS:An observational study of 53 emerging adults (30 males) treated during 2010-2...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-015-0734-z
更新日期:2016-02-01 00:00:00
abstract::Cardiovascular risk is determined by multiple risk factors, all of which greatly increase the chance of morbidity and mortality. So-called "normal" levels of these factors are not biologically normal, so current strategy is based on estimations of a person's global cardiovascular risk, and then using appropriate combi...
journal_title:Acta diabetologica
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s00592-005-0181-3
更新日期:2005-04-01 00:00:00
abstract::Left atrial (LA) structural, functional and mechanical changes have important role in development of diabetic cardiomyopathy and it was discovered that LA remodeling has important prognostic role in the patients with diabetes (DM). Previously the focus of echocardiographic studies in DM population was on the left vent...
journal_title:Acta diabetologica
pub_type: 杂志文章,评审
doi:10.1007/s00592-020-01557-x
更新日期:2020-06-09 00:00:00
abstract:AIMS:Many authors do not recommend hypoglycemic treatment during pregnancy in women affected by monogenic diabetes due to heterozygous glucokinase (GCK) mutations (MODY 2) in case of affected fetus, because maternal hyperglycemia would be necessary to achieve a normal birthweight. We aimed to evaluate differences in bi...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-018-1198-8
更新日期:2018-12-01 00:00:00
abstract::To determine the impact of glargine insulin therapy with different nutritional models on key parameters of diabetes including quality of life, behavior in children and adolescents with type 1 diabetes. Age, duration of diabetes, HbA1c, anthropometric data and episodes of severe hypoglycemia were taken from patients' c...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-007-0019-2
更新日期:2008-03-01 00:00:00
abstract:AIMS:The aims of this study were: to develop new equations for predicting resting energy expenditure (REE) in obese Italian subjects according to body composition parameters; to compare them with predicted values estimated by other REE prediction equations; and to cross-validate our equations using a validation set coh...
journal_title:Acta diabetologica
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00592-017-1061-3
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:International comparisons of diabetes-related lower extremity amputation rates are still hampered by different criteria used for data collection and analysis. We aimed to evaluate trends and variation of major/minor amputations, using agreed definitions adopted by the Organization for Economic Cooperation an...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-019-01423-5
更新日期:2020-03-01 00:00:00
abstract::Hypertriglyceridaemia is associated with insulin resistance of both lipid and glucose metabolism. It is not known whether the insulin resistance affects both glucose oxidation and glycogen formation. To study the oxidative and non-oxidative pathways of non-esterified fatty acids (NEFA) and glucose metabolism, eight ma...
journal_title:Acta diabetologica
pub_type: 临床试验,杂志文章
doi:10.1007/BF00580753
更新日期:1994-04-01 00:00:00
abstract::The objective of the study was to evaluate the association between infectious diseases and other events pertaining to childhood medical history and type 1 diabetes. A case-control study was carried out, taking as cases 159 type 1 diabetic patients (0-29 years) recorded from 1988 to 2000 within the population registry ...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-007-0235-9
更新日期:2007-03-01 00:00:00
abstract::Selective loss of capillary pericytes occurs early and specifically in diabetic retinopathy. We have investigated whether blood derivatives from patients with long-term type 1 (insulin-dependent) diabetes and no retinopathy differ from those with retinopathy and/or non-diabetic controls in their ability to stimulate D...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/BF00572854
更新日期:1993-01-01 00:00:00
abstract::Hemoglobin A1c (HbA1c) has been recommended as an optional method for diagnosing diabetes. The impact of HbA1c on the diagnosis of diabetes has not been evaluated in China, a country with the greatest number of people with diabetes in the world. Hence, we aim to examine how well HbA1c performs as compared with fasting...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-013-0515-5
更新日期:2014-01-01 00:00:00
abstract:AIMS:Glucagon is used to resolve severe hypoglycemia in unconscious patients with diabetes, requiring third-party assistance. A few studies have shown that intranasal (IN) glucagon causes resolution of hypoglycemia in insulin-treated patients with type 1 (T1DM) diabetes. This systematic review and meta-analysis updates...
journal_title:Acta diabetologica
pub_type: 杂志文章,meta分析
doi:10.1007/s00592-020-01483-y
更新日期:2020-06-01 00:00:00
abstract::To study the immune response to glutamic acid decarboxylase (GAD) in insulin-dependent diabetes mellitus, monoclonal GAD antibodies after fusion of splenocytes from a nondiabetes-susceptible BALB/c mouse immunized with human recombinant GAD65 were generated. Of the 44 monoclonals, 35 are specific for the GAD65 isoform...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/BF02048548
更新日期:1996-09-01 00:00:00
abstract:AIM:It is unclear whether any breastfeeding or a certain duration of breastfeeding is protective against the development of diabetes in adult offspring. METHODS:We followed a sub-sample of 3,595 offspring born in the Mater Hospital in Brisbane, Australia between 1981 and 1983 and for whom we had doctor diagnosed self-...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-014-0690-z
更新日期:2015-10-01 00:00:00
abstract::The aim of the study was to evaluate the effects of a supervised physical training added to a healthy diet-rich in either carbohydrate and fibre (CHO/fibre) or monounsaturated fatty acids (MUFA)-on postprandial dyslipidaemia, an independent cardiovascular risk factor particularly relevant in type 2 diabetes (T2D). Par...
journal_title:Acta diabetologica
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00592-013-0522-6
更新日期:2014-01-01 00:00:00
abstract::Insulin resistance is a major factor in the pathogenesis of type 2 diabetes mellitus (T2DM) and is related to the fatty acid profile of the plasma membranes. The purpose of the present study was to investigate fatty acid composition and cholesterol content of cell membranes in patients with type 2 diabetes and, thus, ...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-007-0224-4
更新日期:2006-12-01 00:00:00
abstract::Diabetes mellitus, the most common serious metabolic disorder, is characterized by functional and structural changes in the peripheral and central nervous systems. Glial cells provide structural and metabolic support for retinal neurons. During diabetes, one of the early pathogenic events is retinal glial reactivity. ...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-004-0155-x
更新日期:2004-09-01 00:00:00
abstract:AIMS:Emerging evidence suggested genetic factor attributed as a major determinant for the complex pathogenic mechanism of gestational diabetes mellitus (GDM), but the related genetic study was limited. We aimed to investigate the impact of polymorphisms in miRNA-binding sites (miR-binding SNPs) on the risk of GDM in Ch...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-017-0969-y
更新日期:2017-03-01 00:00:00
abstract::The aim of this study was to analyse the effect of the new oral antidiabetic drug repaglinide on antioxidant factors and lipid peroxidation in tissues of alloxan-induced diabetic rabbits after 4 and 8 weeks treatment. The activity of superoxide dismutase (diabetic vs. control values, mean+/-S.E.M., p<0.05) in diabetic...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-005-0182-2
更新日期:2005-06-01 00:00:00
abstract:BACKGROUND:Despite advances in the medical management of type 1 diabetes mellitus (T1DM), for many, glycaemic control remains substandard. Other factors are clearly important in determining success, or lack thereof, with diabetes management. With this in mind, we have investigated whether family CHAOS may provide a nov...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-015-0736-x
更新日期:2016-02-01 00:00:00
abstract::Insulin secretion is stimulated better by oral than by intravenous glucose (incretin effect). The contribution of the autonomic nervous system to the incretin effect after oral glucose in humans is unclear. We therefore examined nine type 1 diabetic (insulin-dependent) patients with end-stage nephropathy, studied afte...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/BF00572873
更新日期:1993-01-01 00:00:00
abstract:AIMS:To assess the risk of adverse neonatal outcomes in women with gestational diabetes (GDM) by identifying subgroups of women at higher risk to recognize the characteristics most associated with an excess of risk. METHODS:Observational, retrospective, multicenter study involving consecutive women with GDM. To identi...
journal_title:Acta diabetologica
pub_type: 杂志文章,多中心研究
doi:10.1007/s00592-018-1208-x
更新日期:2018-12-01 00:00:00
abstract::Patients with diabetes represent an increasing proportion of end-stage renal disease (ESRD) patients. Cardiovascular risk, already formidable among patients on dialysis, is significantly higher among those who also have diabetes. Diabetic ESRD patients are not only at higher risk of ischaemic events, but are also subj...
journal_title:Acta diabetologica
pub_type: 杂志文章,评审
doi:10.1007/s005920200003
更新日期:2002-04-01 00:00:00