Abstract：
AIMS:Diabetic retinopathy (DR) is characterized by early dropout of capillary pericytes, leading to loss of control on endothelial proliferation and, subsequently, angiogenesis. We have demonstrated that extracellular vesicles (EV) derived from mesenchymal stem cells (MSC) maintained in diabetic-like conditions may play a role in vessel destabilization, thus contributing to angiogenesis through paracrine signalling. In particular, a role for MMP-2 was described. This study was aimed at further investigating the molecular mechanisms of EV-induced vessel destabilization. METHODS:We evaluated miR-126 expression, the subsequent HIF-1α and VEGF modulation, Ang-2 and PDGF signalling pathways in human retinal pericytes (HRP) after exposure to MSC-derived EV obtained in diabetic-like conditions (high glucose and/or hypoxia). RESULTS:HRP express miR-126, and this expression is down-regulated in intermittent high glucose. MSC-derived EV obtained in hyperglycaemic/hypoxic conditions down-regulate miR-126 expression in pericytes, leading to increased expression of angiogenic molecules, such as VEGF and HIF-1α. No modulation of Ang-2 and PDGF signalling pathways in pericytes was observed following EV exposure. CONCLUSIONS:HRP express miR-126, and this expression is down-regulated in diabetic-like conditions. Exposure of HRP to EV obtained in diabetic-like conditions is able to decrease miR-126 expression, consistently with previous observations of its involvement in DR and providing further insights into the role of EV in vessel destabilization. In contrast, PDGF and Ang-2 signalling pathways do not seem to be involved in these mechanisms.
journal_name
Acta Diabetoljournal_title
Acta diabetologicaauthors
Mazzeo A,Beltramo E,Iavello A,Carpanetto A,Porta Mdoi
10.1007/s00592-015-0798-9subject
Has Abstractpub_date
2015-12-01 00:00:00pages
1113-9issue
6eissn
0940-5429issn
1432-5233pii
10.1007/s00592-015-0798-9journal_volume
52pub_type
杂志文章abstract：:Hemoglobin A1c (HbA1c) has been recommended as an optional method for diagnosing diabetes. The impact of HbA1c on the diagnosis of diabetes has not been evaluated in China, a country with the greatest number of people with diabetes in the world. Hence, we aim to examine how well HbA1c performs as compared with fasting...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-013-0515-5
更新日期：2014-01-01 00:00:00
abstract：AIMS:Type 2 diabetes (DM2) is associated to oxidative modifications of high-density lipoproteins (HDL), which can interfere with their function. Pioglitazone has proved effective in raising HDL cholesterol (HDL-C) and lowering small dense low-density lipoprotein (LDL), but no clinical studies have examined its effect o...
journal_title：Acta diabetologica
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00592-018-01278-2
更新日期：2019-05-01 00:00:00
abstract：:Post-prandial hyperglycemia is considered a relevant therapeutic target in type 2 diabetic patients, and it could represent per se an independent risk factor for diabetic complications. Aim of the present systematic review is to collect and summarize evidence from observational studies on the relationship between post...
journal_title：Acta diabetologica
pub_type： 杂志文章,评审
doi：10.1007/s00592-011-0355-0
更新日期：2012-08-01 00:00:00
abstract：:Comparing macrophage-derived cytokine and nitric oxide (NO) profiles in type I and type II diabetes mellitus (DM); and determining whether thymoquinone (TQ) has any modulatory effect were the main objectives of the present study. Peritoneal macrophages have been collected from Otsuka Long-Evans Tokushima Fatty (OLETF)...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-005-0170-6
更新日期：2005-03-01 00:00:00
abstract：AIMS:To assess the risk of adverse neonatal outcomes in women with gestational diabetes (GDM) by identifying subgroups of women at higher risk to recognize the characteristics most associated with an excess of risk. METHODS:Observational, retrospective, multicenter study involving consecutive women with GDM. To identi...
journal_title：Acta diabetologica
pub_type： 杂志文章,多中心研究
doi：10.1007/s00592-018-1208-x
更新日期：2018-12-01 00:00:00
abstract：:Correct classification of diabetic patients in adulthood at the time of diagnosis is often difficult. Some may be initially diagnosed as having non-insulin-dependent diabetes mellitus and be treated with diet and/or oral hypoglycaemic agents (OHA) but later require insulin treatment. Islet cell antibodies and antibodi...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/BF00571567
更新日期：1996-12-01 00:00:00
abstract：:Consistent with the postulated role of oxidative stress in the etiology of late diabetic complications, pharmacological interventions based on biological antioxidants have been suggested. The aim of the present study was to investigate the effect of dietary supplementation with the pyridoindole antioxidant stobadine o...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s005920070012
更新日期：2000-01-01 00:00:00
abstract：AIM:It is unclear whether any breastfeeding or a certain duration of breastfeeding is protective against the development of diabetes in adult offspring. METHODS:We followed a sub-sample of 3,595 offspring born in the Mater Hospital in Brisbane, Australia between 1981 and 1983 and for whom we had doctor diagnosed self-...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-014-0690-z
更新日期：2015-10-01 00:00:00
abstract：:Patients with diabetes represent an increasing proportion of end-stage renal disease (ESRD) patients. Cardiovascular risk, already formidable among patients on dialysis, is significantly higher among those who also have diabetes. Diabetic ESRD patients are not only at higher risk of ischaemic events, but are also subj...
journal_title：Acta diabetologica
pub_type： 杂志文章,评审
doi：10.1007/s005920200003
更新日期：2002-04-01 00:00:00
abstract：:Hyperglycaemia caused by stress and inflammation is common during critical illness. We hypothesised that a latent glucose metabolism disturbance contributes to development of hyperglycaemia and that those patients have increased risk for diabetes. We included patients with sepsis, acute coronary syndrome and acute hea...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-009-0115-6
更新日期：2010-12-01 00:00:00
abstract：:To define whether reference values for bioimpedance analysis (BIA) can be predicted in healthy individuals, individual characteristics and BIA variables (resistance index=height(2)/parallel resistance and reactance index= height(2)/parallel reactance) were evaluated in non-obese healthy individuals: 863 men and 769 wo...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-003-0073-3
更新日期：2003-10-01 00:00:00
abstract：:The objective is to assess the effect of TNF-alpha inhibition on urinary albumin excretion in experimental diabetic rats. Male Wistar rats, 8-week-old, were categorized into four groups, which were the control (n = 9), diabetes (n = 9), infliximab-treated diabetes (n = 10), and FR167653-treated diabetes (n = 9) groups...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-007-0007-6
更新日期：2007-12-01 00:00:00
abstract：AIM:The study evaluated the effect of different concentrations of sildenafil-containing ointment on wound healing in healthy and streptozotocin (STZ)-induced diabetic rats. METHODS:A total of 108 Sprague Dawley male rats aged 5 months were randomly divided into two groups: healthy and diabetes-inducing rats. Following...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-020-01562-0
更新日期：2020-11-01 00:00:00
abstract：:To evaluate the role of insulin resistance and beta cell function to increasing fasting plasma glucose (FPG), 1,272 Chinese subjects (18-80 years of age) were divided into normal glucose tolerance (NGT), impaired fasting glucose (IFG), impaired glucose tolerance (IGT), combined glucose intolerance (CGI), and type 2 di...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-010-0194-4
更新日期：2012-12-01 00:00:00
abstract：BACKGROUND:Psychosocial factors like diabetes distress and social support, as well as the presence of complications, affect an individual's self-management ability; however, their role in adherence behaviours is not yet clear. We examined the role of psychosocial factors and complications in non-adherence behaviours in...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-020-01609-2
更新日期：2020-11-19 00:00:00
abstract：:The aim of this article is to investigate the relation of the anti-inflammatory effect of pioglitazone with cardiac autonomic function and metabolic control in diabetic patients. In this prospective open label trial, 36 type 2 diabetic patients (age 60 ± 10, 20 M) without overt cardiovascular disease were randomized t...
journal_title：Acta diabetologica
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00592-009-0150-3
更新日期：2010-12-01 00:00:00
abstract：:The renin-angiotensin system (RAS) has an important role in the endocrine pancreas. Multiple researches have shown that even in the insulin resistance phase, there are many abnormalities in islets morphology and function. This study aimed at investigating the effects of RAS blockade on the islets function in rats with...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-010-0210-8
更新日期：2013-08-01 00:00:00
abstract：:Endothelial nitric oxide synthase (eNOS) has been shown to play an essential role in retinal vascular function, and disequilibrium in its production can lead to diabetic retinopathy (DR). Genetic polymorphisms of eNOS gene have been suggested to play a role in nitric oxide (NO) abnormalities which may contribute to th...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-012-0437-7
更新日期：2012-12-01 00:00:00
abstract：:The objective of this study was to describe spatial and space-time patterns of type 1 diabetes in children less than 15 years old, diagnosed between 2000 and 2005 with residence in the Metropolitan Region of Chile. Knox and Mantel tests were used to detect space-time interaction between cases. An ecological Bayesian m...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-010-0189-1
更新日期：2010-09-01 00:00:00
abstract：:The purpose of this study was to compare bioelectrical impedance analysis (BIA) to hydrostatic weighing (HW) in male weight lifters and body builders. Twenty-two male body builders and weight lifters, 23 +/- 3 years of age (mean +/- SD), were studied to determine the efficacy of BIA to HW in this population. Subjects ...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-009-0098-3
更新日期：2010-03-01 00:00:00
abstract：:Microalbuminuria in diabetic patients is associated with an increased cardiovascular risk which is not completely explained by an excess of conventional cardiovascular risk factors. A depression of physiologic inhibitors of blood coagulation could contribute to a thrombophilic state and to cardiovascular complications...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/BF00569567
更新日期：1995-06-01 00:00:00
abstract：:We investigated the effect of sub-chronic soybean oil (SO) treatment on the insulin secretion and fatty acid composition of islets of Langerhans obtained from Goto-Kakizaki (GK), a model of type 2 diabetes, and normal Wistar rats. We observed that soybean-treated Wistar rats present insulin resistance and defective is...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-007-0252-8
更新日期：2007-09-01 00:00:00
abstract：:The aim of the present work was to investigate the impact of gender on resting cerebral blood flow velocity and cerebrovascular reserve capacity among diabetic patients. Middle cerebral artery mean blood flow velocity (MCAV) was measured in 72 patients suffering from type 1 diabetes mellitus at rest and 5, 10, 15 and ...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s005920170006
更新日期：2001-01-01 00:00:00
abstract：:Despite advances in technology, the frequent self-measurement of blood glucose (SMBG) remains fundamental to the management of 1 diabetes mellitus (T1DM). Once measured, SMBG results are routinely reported back to health professionals and other interested parties, either verbally, via a logbook, or electronically down...
journal_title：Acta diabetologica
pub_type： 杂志文章,评审
doi：10.1007/s00592-016-0907-4
更新日期：2017-01-01 00:00:00
abstract：:In late twentieth century, Ruderman and Reaven showed that insulin resistance might be fundamental to metabolic syndrome (MetS) which means a constellation of obesity-related metabolic derangements predisposing to type 2 diabetes and cardiovascular disease. In 2001, user-friendly National Cholesterol Education Program...
journal_title：Acta diabetologica
pub_type： 历史文章,杂志文章,评审
doi：10.1007/s00592-011-0309-6
更新日期：2012-04-01 00:00:00
abstract：AIMS:Although diabetes is rapidly increasing in India, there is no national consensus on best practices for screening, diagnosis, and management of gestational diabetes mellitus (GDM). The goal of this study was to systematically review the literature for studies reporting the prevalence and screening and diagnostic me...
journal_title：Acta diabetologica
pub_type： 杂志文章,meta分析,评审
doi：10.1007/s00592-018-1131-1
更新日期：2018-06-01 00:00:00
abstract：:The aim of the study was to evaluate the effects of a supervised physical training added to a healthy diet-rich in either carbohydrate and fibre (CHO/fibre) or monounsaturated fatty acids (MUFA)-on postprandial dyslipidaemia, an independent cardiovascular risk factor particularly relevant in type 2 diabetes (T2D). Par...
journal_title：Acta diabetologica
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00592-013-0522-6
更新日期：2014-01-01 00:00:00
abstract：:Although reactive oxygen species (ROS) contribute to glucose intolerance induced by the renin-angiotensin system (RAS) is well documented, the role of the newly discovered pathway of RAS, angiotensin (Ang)-(1-7)/Mas axis, in this process remains unknown. Here, we examined the effect of Ang-(1-7) on oxidative stress an...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-011-0348-z
更新日期：2012-08-01 00:00:00
abstract：:Continuous subcutaneous insulin infusion (CSII) is effective and safe in children and adults with type 1 diabetes. Notwithstanding, some patients decide to discontinue using CSII. We evaluated the discontinuation rate, and its related factors, in a large group of children and adolescents with type 1 diabetes using CSI...
journal_title：Acta diabetologica
pub_type： 杂志文章,多中心研究
doi：10.1007/s00592-011-0344-3
更新日期：2012-12-01 00:00:00
abstract：AIMS:Radiolabeled Exendin-4 has been proposed as suitable imaging marker for pancreatic beta cell mass quantification mediated by Glucagon-like peptide-1 receptor (GLP-1R). However, noticeable species variations in basal pancreatic uptake as well as uptake reduction degree due to selective beta cell ablation were obser...
journal_title：Acta diabetologica
pub_type： 杂志文章
doi：10.1007/s00592-017-1046-2
更新日期：2017-11-01 00:00:00
