Reporter gene assay for fish-killing activity produced by Pfiesteria piscicida.

Abstract:

:Collaborative studies were performed to develop a functional assay for fish-killing activity produced by Pfiesteria piscicida. Eight cell lines were used to screen organic fractions and residual water fraction by using a 3-[4, 5-dimethylthiazol-(2-4)]-diphenyltetrazolium bromide cytotoxicity assay. Diethyl ether and a residual water fraction were cytotoxic to several cell lines including rat pituitary (GH(4)C(1)) cells. Residual water as well as preextracted culture water containing P. piscicida cells induced c-fos-luciferase expressed in GH(4)C(1) cells with a rapid time course of induction and sensitive detection. The reporter gene assay detected activity in toxic isolates of P. piscicida from several North Carolina estuaries in 1997 and 1998 and may also be suitable for detecting toxic activity in human and animal serum.

authors

Fairey ER,Edmunds JS,Deamer-Melia NJ,Glasgow H Jr,Johnson FM,Moeller PR,Burkholder JM,Ramsdell JS

doi

10.1289/ehp.99107711

subject

Has Abstract

pub_date

1999-09-01 00:00:00

pages

711-4

issue

9

eissn

0091-6765

issn

1552-9924

pii

sc271_5_1835

journal_volume

107

pub_type

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