Abstract:
:The effects of CCK-8s and desulfated CCK-8 at concentrations ranging from 10(-14) to 10(-6) M were studied in vitro on several functions of human peripheral neutrophils: adherence to substrate, mobility (spontaneous and directed by a chemical gradient or chemotaxis), ingestion of inert particles (latex beads) or cells (Candida albicans), and production of superoxide anion measured by the nitroblue tetrazolium reduction test. The effect of CCK-8s on intracellular levels of cAMP was investigated as well as the implication of calcium in the action of CCK-8s on phagocytic function using stimulants and inhibitors of both intracellular and extracellular calcium channels. The two peptides, at concentrations from 10(-12) to 10(-8) M, inhibited significantly both mobility and ingestion capacities and increased adherence to substrate. A dose-response relationship was observed with a maximum inhibition of neutrophil functions at 10(-10) M, CCK-8s and desulfated CCK-8 induced in these cells a significant, but transient, increase of cAMP levels at 60 s. Moreover, CCK-8s was found to inhibit completely the stimulation of latex bead phagocytosis in neutrophils produced by the calcium ionophore A23187. These results suggest that CCK-8 is a negative modulator of several neutrophil functions and that the inhibition of these activities could be carried out through an increase of the intracellular cAMP levels and a decrease of the extracellular calcium input.
journal_name
Peptidesjournal_title
Peptidesauthors
Carrasco M,Del Rio M,Hernanz A,De la Fuente Mdoi
10.1016/s0196-9781(96)00338-5subject
Has Abstractpub_date
1997-01-01 00:00:00pages
415-22issue
3eissn
0196-9781issn
1873-5169pii
S0196-9781(96)00338-5journal_volume
18pub_type
杂志文章相关文献
PEPTIDES文献大全abstract::In order to elucidate the relationship between secretory pathway and processing for precursor molecule of peptide hormones, we performed immunoelectron microscopic studies to localize POMC-derived peptides in mouse cultured L cells (fibroblasts without secretory granules) and in mouse AtT20 cells (ACTH secreting pitui...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(91)90092-4
更新日期:1991-05-01 00:00:00
abstract::Previous reports have demonstrated that systemic injection of cholecystokinin (CCK) in rats produces dose-related decreases in food intake, increases in neurohypophyseal secretion of oxytocin (OT), and decreases in gastric emptying. The present studies determined whether systemic injection of bombesin (BBS), another p...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(88)90194-5
更新日期:1988-11-01 00:00:00
abstract::The first member of the periviscerokinin peptide family in Locusta migratoria was identified by post-source decay fragmentation on a MALDI-TOF mass spectrometer using a single neurohemal organ only. The primary sequence of this decapeptide, code-named Lom-PVK, is Ala-Ala-Gly-Leu-Phe-Gln-Phe-Pro-Arg-Val-NH(2). Unlike t...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(01)00669-6
更新日期:2002-04-01 00:00:00
abstract::Tight junctions (TJ) control paracellular permeability and apical-basolateral polarity of epithelial cells. Dysregulated permeability is associated with pathological conditions, such as celiac disease and inflammatory bowel disease. TJ formation is dependent on E-cadherin-mediated cell-cell adhesion and actin rearrang...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2012.02.016
更新日期:2012-05-01 00:00:00
abstract::Degradation of Boc CCK7 (Boc Tyr1 (SO3H)-Met2-Gly3-Trp4-Met5-Asp6-Phe7-NH2), a fully active analog of CCK8, by purified rabbit kidney neutral metalloendopeptidase (enkephalinase) was studied as a basis for the rational design of potent peptidases-resistant analogs of cholecystokinin. Characterization of the metabolite...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(85)90116-0
更新日期:1985-05-01 00:00:00
abstract::Immunocytochemistry using an antiserum to the C-terminal octapeptide of synenkephalin, proenkephalin(63-70), was performed throughout the rat brain and revealed numerous immunopositive fibers and some cell bodies. The morphology and distribution of synenkephalin immunoreactivity was extremely similar to that of a comm...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(89)90018-1
更新日期:1989-11-01 00:00:00
abstract::Mate attraction in Aplysia involves the long-distance water-borne protein pheromones attractin, enticin, and temptin which are released during egg-laying. Other water-borne pheromones are predicted to act in concert with attractin, enticin, and temptin, but their identities were unknown. We recently identified a highl...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2004.10.024
更新日期:2005-03-01 00:00:00
abstract::Previous studies have shown that different calcitonins interact with planar lipid membranes to form ion channels. In this study, glycosylation of eel calcitonin (eCt) at different positions (Ct3-GlcNAc, Ct14-GlcNAc, Ct20-GlcNAc, Ct26-GlcNAc) is shown to preserve molecular structure and slightly change the energy of in...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2005.09.011
更新日期:2006-04-01 00:00:00
abstract::We studied the expression of the osteogenic and antinociceptive C-terminal histone H4-related peptide fragments, H4-(89-102) (OGP) and H4-(86-100), respectively, within various rat tissues and isolated alveolar macrophages (AM) by radioimmunoassay (RIA). OGP was located mainly within the bone marrow, spleen, thymus, a...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2005.03.013
更新日期:2005-08-01 00:00:00
abstract::Exposure of guinea pigs to the elevated plus maze (X-maze), an animal model of anxiety, causes an increase of extracellular serotonin (5-HT) in the lateral prefrontal cortex monitored by microdialysis. The neuropeptide cholecystokinin (CCK) plays a role in the modulation of anxiety. To compare the roles of CCK recepto...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(97)00454-3
更新日期:1998-01-01 00:00:00
abstract::Using autoradiographic method and 125I-Tyro rat CGRP as a ligand, receptor binding sites were demonstrated in the rat central nervous system. Saturation studies and Scatchard analysis of CGRP-binding to slide mounted tissue sections containing primarily cerebellum showed a single class of receptors with a dissociation...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(85)90331-6
更新日期:1985-09-01 00:00:00
abstract::We examined the effects of tumor necrosis factor (TNF)-alpha on the expression and functionality of adrenomedullin (AM) receptors in cultured human coronary artery smooth muscle cells. Analysis of real-time quantitative polymerase chain reactions showed that these cells abundantly express two AM receptors comprised of...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2004.04.010
更新日期:2004-07-01 00:00:00
abstract::Guinea pig VIP differs from VIP of several mammals by its amino acids in positions 5, 9, 19 and 26. We tested a) its ability to occupy VIP receptors in liver and lung membranes of rat and guinea pig and in the human lymphoblastic SUP-T1 cell line and b) the ensuing adenylate cyclase stimulation. In liver and lung memb...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(91)90180-w
更新日期:1991-01-01 00:00:00
abstract::The identification and characterization of hypothetical membrane proteins from Mycobacterium tuberculosis have led to a better understanding of the mechanisms used by this pathogen to invade and survive inside host cells. This study assessed the presence, transcription, localization and possible biological activity of...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2010.09.017
更新日期:2011-01-01 00:00:00
abstract::Urinary tract infections (UTI) are important health problems and predisposing causes of UTI are not entirely known. Neuro-immune interactions play an important role in human health and disease. Capsaicin-sensitive sensory nerves which in nerve bladder extensively regulate immune system through neuropeptides such as su...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2014.04.003
更新日期:2014-06-01 00:00:00
abstract::The endangered anuran species, Odorrana ishikawae, is endemic to only two small Japanese Islands, Amami and Okinawa. To assess the innate immune system in this frog, we investigated antimicrobial peptides in the skin using artificially bred animals. Nine novel antimicrobial peptides containing the C-terminal cyclic he...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2010.12.013
更新日期:2011-04-01 00:00:00
abstract::Effects of rat and human calcitonin gene-related peptide (r alpha CGRP and h beta CGRP, respectively) upon uterine contractile force were investigated using uterine horns from nonpregnant rats, r alpha CGRP and h beta CGRP were equipotent (pD2 = 8.85-9.09) in inhibiting spontaneous and electrically evoked uterine cont...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(90)90006-q
更新日期:1990-09-01 00:00:00
abstract::This study evaluated the physiological importance of Angiotensin-(1-7) receptor Mas on reflex control of circulation. Experiments were performed in male Mas-knockout (Mas-KO) and Wild Type (WT) conscious mice (12-20 wk of age). Baroreceptor reflex was evaluated by the bradycardic response induced by phenylephrine (0.2...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2010.06.030
更新日期:2010-10-01 00:00:00
abstract::Recent reports suggest that arginine vasopressin (AVP) may be an endogenous antipyretic peptide and a mediator of febrile convulsions [10,12]. The spontaneously seizing Mongolian gerbil was used to investigate the thermoregulatory, behavioral and seizure modulatory effects of AVP. Injection of AVP (1.0 and 5.0 microgr...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(83)90070-0
更新日期:1983-11-01 00:00:00
abstract::The cross-immunoreactivity, topography, and fiber projections of the alpha MSH-immunoreactive specific neurons in the forebrain of the rat appear to be distinctly different from that of the neurons in the hypothalamic arcuate opiocortin system. The cell bodies, immunoreactive only to alpha-MSH, have a specific pattern...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(84)90176-1
更新日期:1984-11-01 00:00:00
abstract::Morphologic and immunohistochemical studies were conducted to ascertain whether pumiliotoxin-B (PTX-B), an indolizine alkaloid from the skin of the Neotropical dendrobatid frog, Dendrobates pumilio, affects the anatomic and immunohistochemical features of the electrically stimulated mouse vas deferens preparations. PT...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(99)00066-2
更新日期:1999-01-01 00:00:00
abstract::Hypothalamic orexin neurons project to many brain areas, including hindbrain structures such as the nucleus of the solitary tract (NTS) and area postrema (AP), where orexin 1 receptors (OX1Rs) are expressed. Hindbrain administration of orexin-A increases feeding and meal size, and blockade of hindbrain OX1Rs with the ...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2020.170351
更新日期:2020-11-01 00:00:00
abstract::Calcitonin gene-related peptide (CGRP) immunoreactive material has been found in extracts of the intestine, however, the structure of intestinal CGRP is not known. Analytical reverse phase HPLC and ion-exchange FPLC revealed one predominant immunoreactive CGRP peak in rabbit intestinal extracts. This material was puri...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(91)90014-g
更新日期:1991-03-01 00:00:00
abstract::Strips of lower esophageal sphincter (LES) from rabbits were tested for their responses to several peptides, and to electrical field stimulation (EFS) under the presence of some peptides. Substance P (SP), motilin, and bombesin induced contraction, and vasoactive intestinal peptide (VIP) induced relaxation. SP- and bo...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(96)00137-4
更新日期:1996-01-01 00:00:00
abstract::We examined the expressions of the prepro-orexin gene in the lateral hypothalamic area (LHA), the genes of the neuropeptide Y (NPY) and proopiomelanocortin (POMC) in the arcuate nucleus (ARC), the orexin type 1 receptor (OX1R) gene in the ventromedial hypothalamic nucleus (VMH) and the orexin type 2 receptor (OX2R) ge...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(02)00111-0
更新日期:2002-09-01 00:00:00
abstract::Due to recent emergence of fungal pathogens resistant to current antifungal therapies, several studies have been focused on screening of plant peptides to find novel compounds having antifungal activities. Here, a novel antifungal plant peptide, with molecular mass of 1230 Da was purified from fruits of Trapa natans b...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2011.06.020
更新日期:2011-08-01 00:00:00
abstract::The distribution of somatostatin (SRIF) was examined in normal human forebrain, using thick vibratome cut sections. The unlabeled antibody enzyme method of immunocytochemistry revealed a widespread distribution of SRIF immunoreactive neurons and fibers throughout the septum, diencephalon and corpus striatum. Within th...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(86)90109-9
更新日期:1986-09-01 00:00:00
abstract::The peptide IIAEK derived from beta-lactoglobulin has a hypocholesterolemic activity greater than that of beta-sitosterol. To create food proteins with multiple copies of this valuable peptide sequence, we introduced tandem multimers of the nucleotide sequence encoding the peptide into DNA regions corresponding to the...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2005.11.007
更新日期:2006-06-01 00:00:00
abstract::Arginine vasopressin (AVP), which is synthesized and secreted in the hypothalamic paraventricular nucleus (PVN), is the most important bioactive substance in the pain modulation. Our pervious study had shown that AVP plays an important role in pain modulation in caudate nucleus (CdN). The experiment was designed to in...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2010.10.014
更新日期:2011-01-01 00:00:00
abstract::Previous studies have shown that the nervous system and other tissues of molluscs contain a number of peptides that potently excite molluscan hearts. Two such peptides, termed small cardioactive peptides A and B (SCPA and SCPB) are present in large quantities in the nervous system of Aplysia. These peptides are widely...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(87)90184-7
更新日期:1987-01-01 00:00:00