Abstract:
:The endangered anuran species, Odorrana ishikawae, is endemic to only two small Japanese Islands, Amami and Okinawa. To assess the innate immune system in this frog, we investigated antimicrobial peptides in the skin using artificially bred animals. Nine novel antimicrobial peptides containing the C-terminal cyclic heptapeptide domain were isolated on the basis of antimicrobial activity against Escherichia coli. The peptides were members of the esculentin-1 (two peptides), esculentin-2 (one peptide), palustrin-2 (one peptide), brevinin-2 (three peptides) and nigrocin-2 (two peptides) antimicrobial peptide families. They were named esculentin-1ISa, esculentin-1ISb, esculentin-2ISa, palustrin-2ISa, brevinin-2ISa, brevinin-2ISb, brevinin-2ISc, nigrocin-2ISa and nigrocin-2ISb. Peptide primary structures suggest a close relationship with the Asian odorous frogs, Odorrana grahami and Odorrana hosii. These antimicrobial peptides possessed a broad-spectrum of growth inhibition against five microorganisms (E. coli, Staphylococcus aureus, methicillin-resistant S. aureus, Bacillus subtilis and Candida albicans). Nine different cDNAs encoding the precursor proteins were also cloned and showed that the precursor proteins exhibited a signal peptide, an N-terminal acidic spacer domain, a Lys-Arg processing site and an antimicrobial peptide at the C-terminus.
journal_name
Peptidesjournal_title
Peptidesauthors
Iwakoshi-Ukena E,Ukena K,Okimoto A,Soga M,Okada G,Sano N,Fujii T,Sugawara Y,Sumida Mdoi
10.1016/j.peptides.2010.12.013subject
Has Abstractpub_date
2011-04-01 00:00:00pages
670-6issue
4eissn
0196-9781issn
1873-5169pii
S0196-9781(10)00541-3journal_volume
32pub_type
杂志文章相关文献
PEPTIDES文献大全abstract::The effects of arginine vasotocin (AVT), 4-6 ng/kg, on renal function and plasma prolactin (PRL) were determined in conscious American foxhounds undergoing a water diuresis. Intravenous AVT produced an antidiuresis, kaliuresis and inconsistent natriuresis, independently of changes in GFR, blood pressure, heart rate an...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(82)90062-6
更新日期:1982-11-01 00:00:00
abstract::Morphologic and immunohistochemical studies were conducted to ascertain whether pumiliotoxin-B (PTX-B), an indolizine alkaloid from the skin of the Neotropical dendrobatid frog, Dendrobates pumilio, affects the anatomic and immunohistochemical features of the electrically stimulated mouse vas deferens preparations. PT...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(99)00066-2
更新日期:1999-01-01 00:00:00
abstract::Previous reports have demonstrated that systemic injection of cholecystokinin (CCK) in rats produces dose-related decreases in food intake, increases in neurohypophyseal secretion of oxytocin (OT), and decreases in gastric emptying. The present studies determined whether systemic injection of bombesin (BBS), another p...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(88)90194-5
更新日期:1988-11-01 00:00:00
abstract::Peptides have the potential to be potent pharmaceutical agents for the treatment of many central nervous system derived maladies. Unfortunately peptides are generally water-soluble compounds that will not enter the central nervous system, via passive diffusion, due to the existence of the blood-brain barrier. Peptides...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/s0196-9781(01)00537-x
更新日期:2001-12-01 00:00:00
abstract::The melanocortin system has been implicated in a multitude of physiological pathways including obesity, satiety, energy homeostasis, sexual behavior, pigmentation, sodium regulation, hypertension, and many others. Based upon studies of the endogenous melanocortin receptor agonists at the cloned human melanocortin rece...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2010.08.025
更新日期:2010-12-01 00:00:00
abstract::The neuropeptide Y system is known to have expanded in early vertebrate evolution. Three neuropeptide Y receptors have been proposed to have existed before the two basal vertebrate tetraploidizations, namely a Y1-like, a Y2-like, and a Y5-like receptor, with their genes in the same chromosomal region. Previously we ha...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2012.11.007
更新日期:2013-01-01 00:00:00
abstract::Studies were conducted in order to determine if selected neurons could be isolated from the brain using Sepharose-linked recognition complexes directed against or related to the biosynthetic/neurosecretory product of the desired neuronal population. Immunoreactive LRF neurons were precipitated when dispersed cells of ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(85)90061-0
更新日期:1985-03-01 00:00:00
abstract::We have previously developed an in vitro model of rat brainstem explants. The latter release sizable amounts of calcitonin gene-related peptide (CGRP); basal release can be stimulated by such secretagogues as high KCl concentrations, veratridine or capsaicine. In this paradigm we investigated the activity of the analg...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2014.03.009
更新日期:2014-06-01 00:00:00
abstract::The goal of our study was to explore the effect of social isolation stress of varying durations on the plasma oxytocin (OT), messenger ribonucleic acid (mRNA) for oxytocin receptor (OTR), plasma arginine vasopressin (AVP) and mRNA for V1a receptor of AVP (V1aR) expression in the hypothalamus and heart of socially mono...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2013.02.007
更新日期:2013-05-01 00:00:00
abstract::The role of the D-Trp residues in the sequence of the NK2-selective tachykinin antagonist, MEN 10207 (Asp-Tyr-D-Trp-Val-D-Trp-D-Trp-Arg-NH2). has been examined by replacement of each D-Trp with either the L-isomer or the residue naturally occurring in the same position of neurokinin A(4-10). The biological activity of...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(91)90053-r
更新日期:1991-09-01 00:00:00
abstract::Various growth factors and their receptors are present in the nervous system. This review focuses on the presence of epidermal growth factor (EGF) and its receptors in the central nervous system (CNS). Evidence indicates that EGF in the CNS is the result of local synthesis, by intrinsic and blood-derived macrophages, ...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/0196-9781(91)90115-6
更新日期:1991-05-01 00:00:00
abstract::High affinity 125I-angiotensin II (Ang II) binding sites were characterized in the canine pancreas. Total binding increased with protein concentration and equilibrium was reached within 60-90 min at 22 degrees C. Specific binding was saturable and averaged 70% of total. Scatchard analysis of binding yielded a KD of 0....
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(92)90114-i
更新日期:1992-03-01 00:00:00
abstract::We have developed a specific and sensitive radioimmunoassay for the hydra head activator neuropeptide (HHAP). The antibody recognizes the C-terminal portion of HHAP and shows no cross-reactivity with other neuropeptides. The assay allows measurement of HHAP-like material in tissue extracts with a minimum detectable co...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(90)90081-f
更新日期:1990-03-01 00:00:00
abstract::Neural membrane fractions, prepared from brain-subesophageal ganglion complexes of the adult lepidopteran Lymantria dispar, contain at least two peptidases capable of metabolizing locust adipokinetic hormone-I in vitro. The initial fragments, pGlu1-Leu2-Asn3 and Phe4-Thr5-Pro6-Asn7-Trp8-Gly9-Thr10, result from the act...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(95)02098-5
更新日期:1996-01-01 00:00:00
abstract::The cervical sympathetic trunk-submandibular gland neuroendocrine axis plays an integral role in physiological adaptations and contributes to the maintenance of systemic homeostasis, particularly under the 'stress conditions' seen with tissue damage, inflammation, and aggressive behavior. The variety of polypeptides, ...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/s0196-9781(00)00158-3
更新日期:2000-03-01 00:00:00
abstract::Circulating urotensin II (UII) concentrations and the tissue expression of its cognate receptor (UT) are elevated in patients with cardiovascular disease (CVD). The functional significance of elevated plasma UII levels in CVD is unclear. Urotensin-related peptide (URP) is a paralog of UII in that it contains the six a...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2007.08.013
更新日期:2008-05-01 00:00:00
abstract::We studied the ability of NPFF, a FMRFamide-like peptide with certain antiopiate properties, to affect the inhibitory effect of morphine on the electrically induced contraction of guinea pig ileum. Neuropeptide FF had no effect when administered alone but reduced morphine inhibition in a noncompetitive manner. Neurope...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(93)90078-u
更新日期:1993-09-01 00:00:00
abstract::Since its discovery, many physiologic functions have been ascribed to ghrelin, a gut derived hormone. The presence of a median fatty acid side chain on the ghrelin peptide is required for the binding and activation of the classical ghrelin receptor, the growth hormone secretagogue receptor (GHSR)-1a. Ghrelin O-acyl tr...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2011.08.020
更新日期:2011-11-01 00:00:00
abstract::We report herein the isolation and characterization of a novel glycine-rich antimicrobial peptide purified from the larvae of Drosophila virilis. A range of chromatographic methods was used for isolation and its antibacterial activity against Bacillus subtilis was employed to screen for the most active fractions. The ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2009.09.028
更新日期:2010-01-01 00:00:00
abstract::Female Sprague-Dawley rat pups were injected SC with either ACTH(1-24) (0.5 mg/kg) or saline vehicle once daily from postnatal day 1 (day of birth) to day 7. Plasma corticosterone (CORT) levels were recorded via radioimmunoassay (RIA) on day 4 to measure adrenal response to these treatments. Hypothalamic 5-HT and DA f...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(93)90056-m
更新日期:1993-03-01 00:00:00
abstract::Previous physiological studies have suggested central roles of brain natriuretic peptide (BNP). However, little information is available about the localization of BNP in the brain. In this study, we determined cDNA sequence encoding the entire coding region of prepro-BNP of Japanese and cynomologus monkeys, and then e...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2006.01.001
更新日期:2006-07-01 00:00:00
abstract::TRH and PS-24 (a TRH peptidase resistant analogue) induce different effects on body temperature and motor activity in rats kept at 4 degrees C. PS-24 induced hypothermia, but TRH did not. PS-24 induced hypermotility, while TRH induced slight hypomotility. The thermal effect of TRH in hypophysectomized rats was similar...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(81)80024-1
更新日期:1981-07-01 00:00:00
abstract::Hepcidin is a peptide hormone regulating iron metabolism, the dyshomeostasis of which has been implicated in dementia. Yet, data on hepcidin status in dementia are scanty, limited to Alzheimer's disease (AD) and inconsistent due to methodological problems with its determination using immunoassays and/or lack of homoge...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2019.170169
更新日期:2019-12-01 00:00:00
abstract::Substance P (SP) is known to be involved in processes related to learning and memory, fear, anxiety and stress. SP and NK1 receptors are localized in the hippocampus, a brain structure involved in learning and memory as well as emotional processes. As there is evidence for differential functions of the ventral (VH) an...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2008.02.014
更新日期:2008-07-01 00:00:00
abstract::Parasitic worms come from two very different phyla-Platyhelminthes (flatworms) and Nematoda (roundworms). Although both phyla possess nervous systems with highly developed peptidergic components, there are key differences in the structure and action of native neuropeptides in the two groups. For example, the most abun...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/s0196-9781(99)00093-5
更新日期:1999-01-01 00:00:00
abstract::HPLC characterization of tilapia pituitary endorphins using an antibody specific for N-terminally acetylated endorphins yielded three major peaks in the neurointermediate lobe, but none in the pars distalis. The melanotropes secreted two of the immunoreactive products in vitro, one of which coeluted with Xenopus laevi...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(94)00204-j
更新日期:1995-01-01 00:00:00
abstract::Of twelve dipeptides tested, only the Trp containing peptides Val-Trp and its reverse peptide Trp-Val showed a xanthine oxidase (XO) inhibitory activity. Studies with Val and Trp revealed that XO inhibition was mainly attributed to the Trp residue. No significant difference (P ≥ 0.05) was found for the XO inhibitory p...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2012.07.030
更新日期:2012-10-01 00:00:00
abstract::The degradation of synthetic Manduca sexta allatostatin (Manse-AS) and allatotropin (Manse-AT), by enzymes of the foregut of larvae of the tomato moth, Lacanobia oleracea was investigated using reversed-phase high performance liquid chromatography (RP-HPLC) together with matrix-assisted laser desorption ionization-tim...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(02)00189-4
更新日期:2002-11-01 00:00:00
abstract::A simple and computationally nonintensive technique based on principal component analysis of 3-dimensional fields to derive theoretical descriptors is presented. The descriptors are then applied to a quantitative structure-activity relationship study on bradykinin potentiating pentapeptides. ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(91)90198-x
更新日期:1991-11-01 00:00:00
abstract::Instability and proteolytic degradation limit the delivery options and in vivo efficacy of many therapeutic peptides. We previously generated a thioether stabilized angiotensin-(1-7) analog, cAng-(1-7), which is resistant against proteolytic degradation in the circulation. We here investigated oral and pulmonary deliv...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2010.02.015
更新日期:2010-05-01 00:00:00