Abstract:
:High affinity 125I-angiotensin II (Ang II) binding sites were characterized in the canine pancreas. Total binding increased with protein concentration and equilibrium was reached within 60-90 min at 22 degrees C. Specific binding was saturable and averaged 70% of total. Scatchard analysis of binding yielded a KD of 0.48 +/- 0.18 nM with a Bmax of 32.8 +/- 6.5 fmol/mg protein (mean +/- SEM, n = 6). The addition of the reducing agent dithiothreitol increased specific binding two-fold. The rank order of displacement of 125I-Ang II binding by native angiotensin peptides was Ang II greater than or equal to Ang III greater than AngI greater than Ang(1-7) much greater than Ang(1-6). The use of the specific Ang II antagonists CGP 42112A, PD 123177, and DuP 753 revealed that the pancreas expresses two receptor subtypes. The majority of Ang II binding sites in the pancreas could be classified as type 2 (AT2), although type 1 (AT1) sites were also detected. In vitro autoradiography revealed binding sites localized over islet cells, acinar and duct cells, as well as the pancreatic vasculature. In addition, the autoradiographic studies confirmed the predominance of the AT2 receptor subtype throughout the pancreas.
journal_name
Peptidesjournal_title
Peptidesauthors
Chappell MC,Diz DI,Jacobsen DWdoi
10.1016/0196-9781(92)90114-isubject
Has Abstractpub_date
1992-03-01 00:00:00pages
313-8issue
2eissn
0196-9781issn
1873-5169pii
0196-9781(92)90114-Ijournal_volume
13pub_type
杂志文章相关文献
PEPTIDES文献大全abstract::Adrenomedullin (AM) is highly expressed in various cancer cell lines, suggesting a possible association with cancer growth. In the present study, we examined the expression and/or concentration of AM, its related peptide, adrenomedullin2/intermedin (AM2/IMD) and their receptors in human colorectal cancer and the surro...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2011.07.012
更新日期:2011-09-01 00:00:00
abstract::Rubiscolin-6 (YPLDLF) is a delta selective opioid peptide isolated from the enzymatic digests of ribulose bisphosphate carboxylase/oxygenase (Rubisco) from spinach leaves. In a step-through type passive avoidance test in ddY mice, rubiscolin-6 enhanced memory consolidation at doses of 3nmol/mouse after intracerebroven...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(03)00044-5
更新日期:2003-02-01 00:00:00
abstract::Plant sucking heteropteran bugs feed regularly on small amounts of K(+)-rich plant material, in contrast to their hematophagous relatives which imbibe large volumes of Na(+)-rich blood. It was anticipated that this would be reflected in the endocrine control of Malpighian tubule (MT) secretion. To explore this, neuroe...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2009.09.017
更新日期:2010-03-01 00:00:00
abstract::Antimicrobial peptides (AMPs) secreted by amphibian skin represent an important innate immune defense strategy. There are more than 340 species in the family of Ranidae worldwidely, and from which nearly 100 families of AMPs comprising between 8 and 48 amino acid (aa) residues have been characterized. In current work,...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2012.01.014
更新日期:2012-02-01 00:00:00
abstract::This work determined Plasmodium falciparum merozoite surface protein-8 (MSP-8) regions specifically binding to membrane surface receptors on human erythrocytes. Five high activity binding peptides (HABPs), whose binding to erythrocytes became saturable and sensitive on being treated with neuraminidase and chymotrypsin...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(03)00185-2
更新日期:2003-07-01 00:00:00
abstract::This is the first report on the structural identity of a neuropeptide of the insect order Megaloptera. A peptide was isolated and sequenced from the retrocerebral corpora cardiaca glands of the alderfly, Sialis lutaria. The sequence of the peptide was deduced from the multiple MS(N) electrospray mass data as that of a...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2008.07.022
更新日期:2009-03-01 00:00:00
abstract::Just as the blood-brain barrier (BBB) is not a static barrier, the adipocytes are not inert storage depots. Adipokines are peptides or polypeptides produced by white adipose tissue; they play important roles in normal physiology as well as in the metabolic syndrome. Adipokines secreted into the circulation can interac...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2007.04.023
更新日期:2007-06-01 00:00:00
abstract::Bombesin (BBS) at doses of 0.1, 1.0, 10.0 and 100.0 nM stimulated chemiluminescence (CL) production by phagocytic cells (monocytes, macrophages and polymorphonuclear leucocytes) in mice in the presence of ZAP (opsonized zymosan particles containing luminol). These data suggest that BBS increased the phagocytic functio...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(90)90098-p
更新日期:1990-03-01 00:00:00
abstract::Amylin is a peptide hormone secreted from the beta cells of the pancreatic islets. Amylin was administered peripherally or centrally following weak or strong training on footshock avoidance conditioning in a T-maze. Under conditions of weak training, amylin improved memory retention in a dose-dependent manner. Under c...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(92)90092-h
更新日期:1992-05-01 00:00:00
abstract::C-type natriuretic peptide (CNP) is assuming increasing importance in cardiovascular disease, and in adults its plasma levels are related to clinical and functional disease severity. Data are scarce regarding the reference values for CNP in infancy. Aim of this study was to assess the reference intervals for CNP in hu...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2011.11.005
更新日期:2012-01-01 00:00:00
abstract::A simple and computationally nonintensive technique based on principal component analysis of 3-dimensional fields to derive theoretical descriptors is presented. The descriptors are then applied to a quantitative structure-activity relationship study on bradykinin potentiating pentapeptides. ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(91)90198-x
更新日期:1991-11-01 00:00:00
abstract::A novel antimicrobial peptide, with molecular mass of 1602.0469Da, produced by Brevibacillus laterosporus strain A60 was isolated and purified from the soil of mango plants. The purification procedure consisted of ammonium sulfate precipitation, cation exchange chromatography on an HiTrap SP HP column, thin layer chro...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2012.01.001
更新日期:2012-02-01 00:00:00
abstract::Gastrin-releasing peptide (GRP) has been implicated in the itch-scratch cycle. We investigated if this gut-brain-skin peptide plays a role in the compulsive, hindleg scratching of the neck of mice by 5'-guanidinonaltrindole (GNTI), the kappa opioid receptor antagonist, and in the antipruritic activity of nalfurafine, ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2010.11.022
更新日期:2011-02-01 00:00:00
abstract::Peptidomic analysis of norepinephrine-stimulated skin secretions of the South-East Asian frog Hylarana erythraea (formerly Rana erythraea partim) has led to the identification of multiple peptides with antimicrobial activity. Structural characterization of the peptides demonstrated that they belong to the brevinin-1 (...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2009.12.013
更新日期:2010-04-01 00:00:00
abstract::The cell adhesion molecule, N-cadherin plays a pivotal role in many biological and disease processes. Drugs that modulate N-cadherin function should therefore be useful therapeutic agents. We have used phage display technology to identify amino acid sequences capable of binding to N-cadherin. All of these sequences ha...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2008.06.025
更新日期:2008-11-01 00:00:00
abstract::Multiple-time regression analysis has been used to study the influx of radiolabeled peptides and polypeptides across the blood-brain barrier (BBB). This study used both tritiated and iodinated leptin to clarify several issues associated with these measurements. Recombinant murine leptin was radiolabeled with 3H by der...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(01)00569-1
更新日期:2001-12-01 00:00:00
abstract::Neuropeptide FF (NPFF) has certain antiopiate actions and may play a role in opiate tolerance and dependence. Third ventricle injection of 10 micrograms NPFF induces a quasimorphine abstinence syndrome in opiate-naive rats. Nitric oxide synthesis may also contribute to opiate tolerance and dependence. The present stud...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(96)00035-6
更新日期:1996-01-01 00:00:00
abstract::Growing evidence suggests that the neuropeptide Y (NPY) system plays an important role in the immune system. Yet, little is known about the expression of NPY and receptors in the immune system. Moreover, original contradicting results have confused the picture and hampered a clear understanding of its role in the immu...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2006.09.030
更新日期:2007-02-01 00:00:00
abstract::To determine if insulin-like growth factor (IGF)-1 and -2, FSH, or leptin alter IGF-binding protein (IGFBP)-2, -3, -4, and -5 mRNA levels in bovine granulosa and (or) theca cells, granulosa and theca cells were collected from bovine ovarian follicles, plated for 48 h in 10% FCS and then treated for 24 h in serum-free ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2004.07.008
更新日期:2004-12-01 00:00:00
abstract::A series of Locusta adipokinetic hormone I (AKH-I), < QLNFTPNWGTa, analogues, were synthesized with modifications at the C-terminal threonine residue using a combination of solid- and liquid-phase methodology and evaluated in Locusta migratoria, in a lipid mobilization assay in vivo and an acetate uptake assay in vitr...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(96)00224-0
更新日期:1996-01-01 00:00:00
abstract::Intracerebroventricular injections of rat hypothalamic growth hormone-releasing factor (rhGRF) were examined for effects on food intake in free-feeding rats. It was found that, in picomole doses, rhGRF had a stimulatory effect on food intake. Examination of higher doses of rhGRF revealed a decrease in the facilitatory...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(88)90220-3
更新日期:1988-01-01 00:00:00
abstract::Mastoparan (MP) peptides, distributed in insect venoms, induce a local inflammatory response post envenomation. Most endogenous MPs share common structural elements within a tetradecapeptide sequence that adopts an amphipathic helix whilst traversing biological membranes and when bound to an intracellular protein targ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2018.01.007
更新日期:2018-03-01 00:00:00
abstract::The present status of our understanding of the feedback regulation of pancreatic secretion by peptide YY (PYY) released from the distal intestine is reviewed. Exocrine pancreatic secretion is primarily controlled by the cephalic (the vagus nerve), gastric (acid and pepsin secretion, and nutrients delivered into the du...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/s0196-9781(01)00612-x
更新日期:2002-02-01 00:00:00
abstract::The first member of the periviscerokinin peptide family in Locusta migratoria was identified by post-source decay fragmentation on a MALDI-TOF mass spectrometer using a single neurohemal organ only. The primary sequence of this decapeptide, code-named Lom-PVK, is Ala-Ala-Gly-Leu-Phe-Gln-Phe-Pro-Arg-Val-NH(2). Unlike t...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(01)00669-6
更新日期:2002-04-01 00:00:00
abstract::The amino acid sequence of the vasoactive intestinal polypeptide (VIP) is well conserved between species. Thus, all mammalian VIPs isolated so far, except that of the guinea pig, have the same amino acid sequence. This study describes the isolation and primary structure of sheep brain VIP. The purification was followe...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(90)90184-7
更新日期:1990-07-01 00:00:00
abstract::Honeybee stings are a severe public health problem. Bee venom contains a series of active components, including enzymes, peptides, and biogenic amines. The local reactions observed after envenoming include a typical inflammatory response and pain. Honeybee venom contains some well-known polycationic peptides, such as ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2014.07.004
更新日期:2014-09-01 00:00:00
abstract::Previously, we described a gene, flp-1, that encodes seven FMRFamide-like peptides from two alternatively spliced transcripts in the nematode Caenorhabditis elegans. To determine whether all or a subset of the predicted peptides coded for by flp-1 are produced in vivo, we undertook the isolation of FMRFamide-like pept...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(93)90049-m
更新日期:1993-03-01 00:00:00
abstract::Trefoil factors family 2 (TFF2), also known as spasmolytic polypeptide, is primarily expressed in the mucus neck cells of gastrointestinal tracts. It has been proposed that TFF2 plays an important physiological role in protection, repair, and healing of gastrointestinal mucosa. To investigate the cis-acting regulatory...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2003.11.022
更新日期:2004-05-01 00:00:00
abstract::The distribution of corazonin in the central nervous system of the heteropteran insect Triatoma infestans was studied by immunohistochemistry. The presence of corazonin isoforms was investigated using MALDI-TOF mass spectrometry in samples containing the brain, the subesophageal ganglion, the corpora cardiaca-corpus a...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2010.10.020
更新日期:2011-03-01 00:00:00
abstract::Intracerebroventricular administration of NPY suppresses ethanol intake in selectively bred alcohol-preferring rat lines, but not in rats selectively bred for low ethanol drinking or in unselected Wistar rats, when access to ethanol is limited to 2h/day. However, when rats undergo chronic (24h/day) ethanol drinking (o...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2006.07.028
更新日期:2007-02-01 00:00:00