Abstract:
:Degradation of Boc CCK7 (Boc Tyr1 (SO3H)-Met2-Gly3-Trp4-Met5-Asp6-Phe7-NH2), a fully active analog of CCK8, by purified rabbit kidney neutral metalloendopeptidase (enkephalinase) was studied as a basis for the rational design of potent peptidases-resistant analogs of cholecystokinin. Characterization of the metabolites was performed by HPLC using several elution procedures. Three cleavage sites were evidenced: one major at the Asp6-Phe7 bond and two minor at Gly3-Trp4 and Trp4-Met5 bonds. All cleavages were fully inhibited by thiorphan, a potent inhibitor of enkephalinase. The relative importance of the different cleavages was established using several cholecystokinin analogs. At 25 degrees C the half-disappearance time was 18 min for Boc CCK7, Boc[diNle2,5]CCK7 and 70 min for Boc[diNle2,5 D.Asp6]CCK7. Although, half-life of Boc CCK7 and Boc[diNle2,5]CCK7 were identical, the replacement of Met by Nle, a more hydrophobic aminoacid, greatly favoured the cleavage at the Trp4-Nle5 bond which became the major breakdown. This feature was exemplified by the substitution of L.Asp by D.Asp, preventing the Trp4-Nle5 cleavage, which gave rise to the most enkephalinase-resistant analog in this series.
journal_name
Peptidesjournal_title
Peptidesauthors
Durieux C,Charpentier B,Fellion E,Gacel G,Pelaprat D,Roques BPdoi
10.1016/0196-9781(85)90116-0subject
Has Abstractpub_date
1985-05-01 00:00:00pages
495-501issue
3eissn
0196-9781issn
1873-5169pii
0196-9781(85)90116-0journal_volume
6pub_type
杂志文章相关文献
PEPTIDES文献大全abstract::Androgenic gland hormone (AGH) is known to be responsible for sex differentiation in crustaceans. The amino acid sequence of AGH-active fraction purified from androgenic glands of the terrestrial isopod Armadillidium vulgare was determined by immunoprecipitation employing three types of antibodies raised against diffe...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(00)00374-0
更新日期:2001-02-01 00:00:00
abstract::Previously, we described a gene, flp-1, that encodes seven FMRFamide-like peptides from two alternatively spliced transcripts in the nematode Caenorhabditis elegans. To determine whether all or a subset of the predicted peptides coded for by flp-1 are produced in vivo, we undertook the isolation of FMRFamide-like pept...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(93)90049-m
更新日期:1993-03-01 00:00:00
abstract::Human Zonulin, related to the Zonula occludens toxin of Vibrio cholerae, regulates intestinal permeability and is induced in inflammatory disorders of the lower GI tract. Gastroesophageal reflux disease (GERD) is associated with an impairment of epithelial barrier function. Here, we studied expression of zonulin in th...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2009.03.017
更新日期:2009-06-01 00:00:00
abstract::Since its discovery, many physiologic functions have been ascribed to ghrelin, a gut derived hormone. The presence of a median fatty acid side chain on the ghrelin peptide is required for the binding and activation of the classical ghrelin receptor, the growth hormone secretagogue receptor (GHSR)-1a. Ghrelin O-acyl tr...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2011.08.020
更新日期:2011-11-01 00:00:00
abstract::Modification to the structure of glucagon has provided a number of glucagon receptor antagonists with possible therapeutic application for diabetes. These novel peptide analogs include desHis(1)Pro(4)Glu(9)-glucagon and desHis(1)Pro(4)Glu(9)(Lys(30)PAL)-glucagon. This study has evaluated the metabolic benefits of once...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2014.08.002
更新日期:2014-10-01 00:00:00
abstract::Nervous, endocrine, and immune systems share a large number of regulatory molecules including hormones and neuropeptides. Vasoactive intestinal peptide (VIP) plays an important role in a variety of immunological functions. In the present report, we sorted purified thymocytes of the four major thymic subsets defined by...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(96)00070-8
更新日期:1996-01-01 00:00:00
abstract::Hypothalamic orexin neurons project to many brain areas, including hindbrain structures such as the nucleus of the solitary tract (NTS) and area postrema (AP), where orexin 1 receptors (OX1Rs) are expressed. Hindbrain administration of orexin-A increases feeding and meal size, and blockade of hindbrain OX1Rs with the ...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2020.170351
更新日期:2020-11-01 00:00:00
abstract::The first member of the periviscerokinin peptide family in Locusta migratoria was identified by post-source decay fragmentation on a MALDI-TOF mass spectrometer using a single neurohemal organ only. The primary sequence of this decapeptide, code-named Lom-PVK, is Ala-Ala-Gly-Leu-Phe-Gln-Phe-Pro-Arg-Val-NH(2). Unlike t...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(01)00669-6
更新日期:2002-04-01 00:00:00
abstract::The bursa of Fabricius (BF) is acknowledged as central humoral immune organ unique to birds. Our purpose was to identify the potential function of a novel bursal-derived bioactive peptide. A bursal septpeptide (BSP-I), EPASGMM, first isolated from BF, reduced MCF and Hela tumor cells proliferation, and enhanced antitu...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2011.04.020
更新日期:2011-06-01 00:00:00
abstract::The opioid peptides, [3H]DAGO and [3H]DPDPE, bound to rat and guinea pig brain homogenates with a high, nanomolar affinity and to a high density of mu and delta receptors, respectively. [3H]DAGO binding to mu receptors was competitively inhibited by unlabelled opioids with the following rank order of potency: DAGO gre...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(89)90135-6
更新日期:1989-05-01 00:00:00
abstract::We investigated the plasma concentration of ghrelin peptide during pregnancy and lactation in rats. Plasma ghrelin levels on days 10 and 15 of pregnancy were significantly lower than those of the non-pregnant rats. Thereafter, the plasma ghrelin levels on day 20 of pregnancy sharply increased to levels comparable with...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2004.01.011
更新日期:2004-02-01 00:00:00
abstract::Peptide YY (PYY)(3-36), a neuropeptide Y (NPY) Y2 receptor agonist, is a powerful inhibitor of intestinal secretion. Based on this anti-secretory effect, NPY Y2 receptor agonists may be useful as novel anti-diarrheal agents, but anti-diarrheal efficacy has yet to be determined. We therefore examined the anti-diarrheal...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2009.11.005
更新日期:2010-04-01 00:00:00
abstract::The effects of arginine vasotocin (AVT), 4-6 ng/kg, on renal function and plasma prolactin (PRL) were determined in conscious American foxhounds undergoing a water diuresis. Intravenous AVT produced an antidiuresis, kaliuresis and inconsistent natriuresis, independently of changes in GFR, blood pressure, heart rate an...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(82)90062-6
更新日期:1982-11-01 00:00:00
abstract::Short synthetic peptides corresponding to sequences of complementarity-determining regions (CDRs) from different immunoglobulin families have been shown to induce antimicrobial, antiviral and antitumor activities regardless of the specificity of the original monoclonal antibody (mAb). Presently, we studied the in vitr...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2014.06.007
更新日期:2014-09-01 00:00:00
abstract::Animal behavioral and neurochemical studies implicate dopaminergic systems in the neurological sequelae induced by estrogen. In the present study, we demonstrated for the first time that MIF-1, a neuropeptide unrelated to classical dopamine agonists, when given prior to, concurrently with, and after 17 beta-estradiol,...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(87)90127-6
更新日期:1987-11-01 00:00:00
abstract::The cellular distribution of PP and PYY in mammals is reviewed. Expression of PP is restricted to endocrine cells mainly present in the pancreas predominantly in the duodenal portion (head) but also found in small numbers in the gastro-intestinal tract. PYY has a dual expression in both endocrine cells and neurons. PY...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/s0196-9781(01)00601-5
更新日期:2002-02-01 00:00:00
abstract::Marine snails of the genus Conus (∼500 species) are tropical predators that produce venoms for capturing prey, defense and competitive interactions. These venoms contain 50-200 different peptides ("conotoxins") that generally comprise 7-40 amino acid residues (including 0-5 disulfide bridges), and that frequently cont...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2014.01.018
更新日期:2014-03-01 00:00:00
abstract::Neuropeptide S (NPS), the endogenous ligand of NPS receptor (NPSR), regulates many biological functions, including arousal, anxiety, locomotion and food intake. NPSR mRNA is expressed in several regions of central autonomic network through which the brain controls visceromotor and other responses essential for surviva...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2009.03.012
更新日期:2009-07-01 00:00:00
abstract::Opiate binding sites in five brain regions were labeled with the mu and delta markers, 3H-morphine and 3H-[D-Ala2,D-leu5]enkephalin, respectively. The highest densities of both 3H-morphine and 3H-DADLE labeled sites are found in striatum and frontal cortex. Hypothalamus and midbrain contain predominantly 3H-morphine l...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(83)90080-3
更新日期:1983-11-01 00:00:00
abstract::Unilateral intracerebroventricular (ICV) injections of a high dose of CCK7 have been reported to elicit barrel rotations accompanied by contralateral postural asymmetry; there was no spontaneous locomotor activity other than barrel rolling. The aim of the present study was to investigate the effects of lower doses of ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(87)90126-4
更新日期:1987-11-01 00:00:00
abstract::This review describes the role of modulation of intracellular adhesion molecule-1 (ICAM-1)/leukocyte function-associated antigen-1 (LFA-1) interaction in controlling autoimmune diseases or inducing immunotolerance. ICAM-1/LFA-1 interaction is essential for T-cell activation as well as for migration of T-cells to targe...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/s0196-9781(03)00083-4
更新日期:2003-03-01 00:00:00
abstract::Paramyosin of the pig-human parasite Taenia solium (TPmy) is a α-helical protein located on the worm surface that is suggested to fulfill an immunomodulatory role protecting the parasite against host immune system. Besides, in challenging experiments the protein shows a vaccine potential. These observations imply that...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2011.10.003
更新日期:2012-03-01 00:00:00
abstract::GIP receptor knockout mice were shown to be protected from the development of obesity on a high fat diet, suggesting a role of GIP in the development of obesity. In our study we aimed to test the hypothesis if excess of GIP could accelerate development of obesity and to identify GIP gene targets in adipose tissue. The...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2011.02.011
更新日期:2011-05-01 00:00:00
abstract::The cervical sympathetic trunk-submandibular gland neuroendocrine axis plays an integral role in physiological adaptations and contributes to the maintenance of systemic homeostasis, particularly under the 'stress conditions' seen with tissue damage, inflammation, and aggressive behavior. The variety of polypeptides, ...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/s0196-9781(00)00158-3
更新日期:2000-03-01 00:00:00
abstract::Many mammalian brain-gut peptides are known to be represented in invertebrate nervous systems; we have now examined the possibility that an invertebrate neuropeptide occurs in vertebrates. Antisera were raised in rabbits to the molluscan neuropeptide. Phe-Met-Arg-Phe-NH2 (FMRFamide). The antiserum used for radioimmuno...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/0196-9781(81)90005-x
更新日期:1981-01-01 00:00:00
abstract::Medium conditioned by a highly enriched population of gonadotrophs, cultured as reaggregates in the presence of 0.01 nM GnRH, was concentrated, separated on a reversed-phase HPLC column, and tested for activity on lactotroph development in pituitary reaggregate cell cultures of 14-day-old rats. The number of cells exp...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(96)00175-1
更新日期:1996-01-01 00:00:00
abstract::Evidence on the relative roles of endothelin ET(A) and ET(B) receptors in mediating the nociceptive and hyperalgesic actions of endothelin-1 is still fragmented and conflicting, due to variations between species and/or models. This study assesses the participation of ET(A) and ET(B) receptors on the nociceptive behavi...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2009.01.011
更新日期:2009-05-01 00:00:00
abstract::New dual immunocytochemical staining procedures were used in the same tissue section to elucidate the distribution and co-existence of CRF and vasopressin in parvocellular neuronal perikarya in the paraventricular nucleus (PVN) of rat hypothalamus. CRF immunostained cells were for the most part concentrated in the med...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(86)90111-7
更新日期:1986-09-01 00:00:00
abstract::Intracerebroventricular injections of rat hypothalamic growth hormone-releasing factor (rhGRF) were examined for effects on food intake in free-feeding rats. It was found that, in picomole doses, rhGRF had a stimulatory effect on food intake. Examination of higher doses of rhGRF revealed a decrease in the facilitatory...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(88)90220-3
更新日期:1988-01-01 00:00:00
abstract::To examine the relationship between glutamate receptors and the action of NC-1900 on a step-through passive avoidance (PA) task in mice, MK-801, an NMDA receptor blocker, and (S)-4-carboxyphenylglycine (4CPG), a group I metabotropic receptor antagonist, were administered intraventricularly (i.c.v.) singly or as co-inj...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2004.12.014
更新日期:2005-05-01 00:00:00