Abstract:
:Crest-derived glomus cells of the carotid body (CB) are O(2)-sensitive chemoreceptors, which resemble sympathoadrenal (SA) chromaffin cells. In this study, we tested whether perinatal rat glomus cells are sensitive to basic fibroblast growth factor (bFGF) in vitro and whether their sensitivity is regulated by oxygen. In chemically defined medium, bFGF (1-100 ng/ml) caused a significant, dose-dependent increase in the number of surviving tyrosine hydroxylase-positive (TH+) glomus cells in embryonic (E17-E19) CB cultures, following a 48-hr exposure. Though basic FGF (10 ng/ml) appeared mitogenic for these cells, based on stimulation of bromodeoxyuridine (BrdU) uptake, it supported survival of only approximately 60% of the initial TH+ population, suggesting that significant cell death was occurring. This apparent cell loss in E17 cultures could be largely prevented by combined treatment with bFGF and low oxygen (6% O(2)). In contrast, in early postnatal (P1) cultures, glomus cell number was relatively unchanged over 48 hr under control conditions or in presence of mitogenic activity from either bFGF or low oxygen. However, combined treatment with both bFGF and low oxygen stimulated proliferation of P1 glomus cells such that by 48 hr the TH+ population had increased to approximately 1.5x the initial density. Basic FGF (10 ng/ ml) also stimulated neurite outgrowth and neurofilament expression in E18-E19, but not P1-P3, glomus cells. In contrast to bFGF, treatment with nerve growth factor was ineffective. Taken together, these results suggest that bFGF and low oxygen are mitogens for perinatal CB chromaffin cells and interact cooperatively as survival factors. It is plausible that these mechanisms may operate to regulate chemoreceptor cell density, during the animal's transition from in utero (hypoxic) to ex utero (normoxic)life.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Nurse CA,Vollmer Cdoi
10.1006/dbio.1997.8539subject
Has Abstractpub_date
1997-04-15 00:00:00pages
197-206issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(97)98539-0journal_volume
184pub_type
杂志文章abstract::The MEI-1/MEI-2 microtubule-severing complex, katanin, is required for oocyte meiotic spindle formation and function in C. elegans, but the microtubule-severing activity must be quickly downregulated so that it does not interfere with formation of the first mitotic spindle. Post-meiotic MEI-1 inactivation is accomplis...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.04.004
更新日期:2009-06-15 00:00:00
abstract::Thrombospondin type 1 repeat (TSR) superfamily members regulate diverse biological activities ranging from cell motility to inhibition of angiogenesis. In this study, we verified that mouse protein O-fucosyltransferase-2 (POFUT2) specifically adds O-fucose to TSRs. Using two Pofut2 gene-trap lines, we demonstrated tha...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.07.008
更新日期:2010-10-01 00:00:00
abstract::Using two different models of pregnancy failure in mice, we show in this communication that preimplantation embryonic development can be controlled, in vivo, with cytokines. In one model, described by us previously, CSF-1 is shown to block gestations in plugged females due to its induction of defective early developme...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90236-v
更新日期:1991-08-01 00:00:00
abstract::Chromatin regulators contribute to the developmental control of gene expression. In the nematode Caenorhabditis elegans, the roles of chromatin regulation in development have been explored in several contexts, including vulval differentiation. The synthetic multivulva (synMuv) genes are regulators of vulval developmen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.05.018
更新日期:2014-08-15 00:00:00
abstract::Salivary gland formation in the Drosophila embryo is dependent on the homeotic gene Sex combs reduced (Scr). When Scr function is missing, salivary glands do not form, and when SCR is expressed everywhere in the embryo, salivary glands form in new places. Scr is normally expressed in all the cells that form the saliva...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9560
更新日期:2000-01-15 00:00:00
abstract::The HCO3(-) anion activates sperm motility, an important early step in capacitation, by increasing flagellar beat frequency through a pathway that requires the atypical adenylyl cyclase SACY and the sperm-specific C alpha2 catalytic subunit of PKA. Here we show that the accelerating action of HCO3(-) also requires the...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.09.017
更新日期:2007-12-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90441-6
更新日期:1987-09-01 00:00:00
abstract::We recently demonstrated a critical role for two-pore channel type 2 (TPC2)-mediated Ca2+ release during the differentiation of slow (skeletal) muscle cells (SMC) in intact zebrafish embryos, via the introduction of a translational-blocking morpholino antisense oligonucleotide (MO). Here, we extend our study and demon...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.03.031
更新日期:2017-05-15 00:00:00
abstract::We defined the cellular mechanisms for genesis, migration, and differentiation of the initial population of olfactory bulb (OB) interneurons. This cohort of early generated cells, many of which become postmitotic on embryonic day (E) 14.5, differentiates into a wide range of mature OB interneurons by postnatal day (P)...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.05.009
更新日期:2006-09-15 00:00:00
abstract::Octopamine is a major monoamine in invertebrates and affects many physiological processes ranging from energy metabolism to complex behaviors. Octopamine binds to receptors located on various cell types and activates distinct signal transduction pathways to produce these diverse effects. We previously identified one o...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2003.07.018
更新日期:2003-12-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0219
更新日期:1996-09-15 00:00:00
abstract::Chondrocytes were isolated from the auricular cartilage of rabbits, aged 1 week to 30 months, and grown in short-term cell culture. The cells from the 1-week animals were small, polygonal, and mononucleated, while the chondrocytes from the older animals were larger, rounded, and frequently binucleated. The synthesis o...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90311-1
更新日期:1983-03-01 00:00:00
abstract::The cardiac neural crest contains ectomesenchymal and neural anlagen that are necessary for normal heart development. It is not known whether other regions of the neural crest are capable of supporting normal heart development. In the experiments reported herein, quail donor embryos provided cardiac, trunk, or mesence...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90112-7
更新日期:1989-08-01 00:00:00
abstract::Cell-cell fusion is a fundamental cellular process that is essential for development as well as fertilization. Myoblast fusion to form multinucleated skeletal muscle myotubes is a well studied, yet incompletely understood example of cell-cell fusion that is essential for formation of contractile skeletal muscle tissue...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.10.035
更新日期:2009-01-15 00:00:00
abstract::Previous experiments have demonstrated that in the septo-hippocampal system choline acetyltransferase (ChAT) is induced by nerve growth factor (NGF) (Gnahn et al. (1983) Dev. Brain Res. 9, 45-52) and that hippocampal NGF and mRNANGF levels are correlated with the density of cholinergic innervation (Korsching et al. (1...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90235-1
更新日期:1987-04-01 00:00:00
abstract::Tbx18 has been shown to be essential for ureteral development. However, it remains unclear whether it plays a direct role in kidney development. Here we addressed this by focusing on examining the pattern and contribution of Tbx18+ cells in the kidney and its role in kidney vascular development. Expression studies and...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.04.006
更新日期:2014-07-01 00:00:00
abstract::Characterization of the molecular pathways controlling differentiation and proliferation in mammalian hair follicles is central to our understanding of the regulation of normal hair growth, the basis of hereditary hair loss diseases, and the origin of follicle-based tumors. We demonstrate that the proto-oncogene Wnt3,...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.9140
更新日期:1999-03-01 00:00:00
abstract::We have examined the presence of protein kinase C in oocytes of Chaetopterus pergamentaceus and its role in the initiation of germinal vesicle breakdown (GVBD). First, we demonstrated that the oocytes contain a phospholipid- and calcium-dependent protein kinase, protein kinase C (PKC). Since PKC is the primary intrace...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90294-q
更新日期:1992-02-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:
更新日期:2002-10-15 00:00:00
abstract::In this report we describe the development of a sensitive assay for gene expression in zebrafish embryos using beta-lactamase as a reporter gene. We show that injection of a green fluorescent substrate for beta-lactamase allows the detection of reporter gene expression in live embryos. The beta-lactamase enzyme cataly...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8999
更新日期:1998-11-15 00:00:00
abstract::We have isolated two related Xenopus homologues of the homeotic zinc finger protein Teashirt1 (Tsh1), XTsh1a and XTsh1b. While Drosophila teashirt specifies trunk identity in the fly, the developmental relevance of vertebrate Tsh homologues is unknown. XTsh1a/b are expressed in prospective trunk CNS throughout early n...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.06.041
更新日期:2006-10-01 00:00:00
abstract::The segmentation of somites from the paraxial mesoderm is a crucial event in vertebrate embryonic development; however, the mechanisms underlying this process are not well understood. In a yeast two-hybrid screen we have identified the novel basic-helix-loop-helix (bHLH) protein cMeso-1 which is expressed in the preso...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8919
更新日期:1998-07-15 00:00:00
abstract::Single cells from 512-cell Xenopus embryos, totally labeled with intracellular horseradish peroxidase, were transplanted orthotopically (from either dorsal or lateral marginal zone) or heterotopically (between these two marginal zones) to unlabeled host embryos at the 512-cell stage. At tailbud stage 23 the locations,...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(89)80043-0
更新日期:1989-01-01 00:00:00
abstract::About 12-17% of the embryos obtained by mating mice carrying the In(X)1H or Paf mutations are of the 39,X (X0) genotype. Depending on the mutant mice used for mating, the monosomic X chromosome can be inherited from the paternal (XP) or the maternal (XM) parent. The XP0 embryos display developmental retardation at gas...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8972
更新日期:1998-09-01 00:00:00
abstract::The Wnt pathways are evolutionarily well-conserved signal transduction pathways that are known to play important roles in all Metazoans investigated to date. Here, we examine the Wnt pathway genes and target genes present in the genome of the echinoderm Strongylocentrotus purpuratus. Analysis of the Wnt genes revealed...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.08.045
更新日期:2006-12-01 00:00:00
abstract::The Smcp mRNA encoding the sperm mitochondria-associated cysteine-rich protein is translationally repressed in round spermatids and translationally active in elongated spermatids. The patterns of transcription and translation of fusions of the Smcp promoter, the green fluorescent protein coding region (Gfp) and variou...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.04.468
更新日期:2006-09-01 00:00:00
abstract::Progression of germ cells through meiosis is regulated by phosphorylation events. We previously showed the key role of cyclin dependent kinases in meiotic divisions of rat spermatocytes co-cultured with Sertoli cells (SC). In the present study, we used the same culture system to address the role of mitogen-activated p...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.12.019
更新日期:2008-03-01 00:00:00
abstract::The 3' penultimate exon (exon 18) of transcripts from the muscle myosin heavy chain (MHC) gene of Drosophila melanogaster is excluded from mRNAs of embryonic and larval muscle, while it is included in mRNAs of adult thoracic muscles. By transforming organisms with the MHC gene 5' end, linked in-frame to the MHC gene 3...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90235-u
更新日期:1991-08-01 00:00:00
abstract::Notch signaling inhibits hair cell differentiation, based on studies on mice deficient in Notch signaling-related genes and its downstream genes. However, the precise mechanisms of this inhibition are unknown because it is difficult to control the timing and duration of the suppression of Notch signaling. Here, we dev...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.04.035
更新日期:2007-07-01 00:00:00
abstract::Embryonic development involves a series of cell adhesive interactions that provide mechanical and instructive information required for morphogenesis. The ADAMs family of membrane-anchored proteins, containing a disintegrin and metalloprotease domain, is well suited for participating in such developmental events. They ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.8458
更新日期:1997-02-15 00:00:00