The 5' UTR and 3' UTR of the sperm mitochondria-associated cysteine-rich protein mRNA regulate translation in spermatids by multiple mechanisms in transgenic mice.


:The Smcp mRNA encoding the sperm mitochondria-associated cysteine-rich protein is translationally repressed in round spermatids and translationally active in elongated spermatids. The patterns of transcription and translation of fusions of the Smcp promoter, the green fluorescent protein coding region (Gfp) and various combination of the Smcp and Gfp 5' UTR and 3' UTR have been studied in transgenic mice. 518 nt of Smcp 5' flanking region and 8 nt of 5' UTR drive transcription of mRNAs containing the Gfp coding region in early round spermatids at the same transcription start site as the natural Smcp gene. Transcripts containing both the Gfp 5' and 3' UTRs are translationally active in step 2 spermatids as detected by GFP fluorescence in squashes of living seminiferous tubules from adult testes, and the presence of polysomal mRNAs in sucrose gradient analyses of testes from 21-day-old prepubertal mice, which contain early round spermatids and lack elongated spermatids. By comparison, expression of GFP is delayed until steps 5 and 10, respectively, in transcripts containing the Smcp 5' UTR and Gfp 3' UTR and the Gfp 5' UTR and the Smcp 3' UTR. Sucrose gradient analysis of 21-day-old testes demonstrates that transcripts containing the Smcp 3' UTR exhibit a bimodal distribution in free-mRNPs and polysomes, indicating that the 3' UTR blocks the expression of GFP after the transcripts have entered the elongation phase, a mechanism that may involve microRNAs. The Smcp 5' UTR reduces the levels and size of polysomes in adult testis. In addition, the natural Smcp mRNA contains a positive control element that counteracts the inhibition of translation by the Smcp 5' UTR in adult testis, and the Smcp 3' UTR strongly localizes GFP fluorescence in step 10 spermatids.


Dev Biol


Developmental biology


Hawthorne SK,Busanelli RR,Kleene KC




Has Abstract


2006-09-01 00:00:00














  • A newly identified type of attachment cell is critical for normal patterning of chordotonal neurons.

    abstract::This work describes unknown aspects of chordotonal organ (ChO) morphogenesis revealed in post-embryonic stages through the use of new fluorescently labeled markers. We show that towards the end of embryogenesis a hitherto unnoticed phase of cell migration commences in which the cap cells of the ventral ChOs elongate a...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Halachmi N,Nachman A,Salzberg A

    更新日期:2016-03-01 00:00:00

  • Changes in endoplasmic reticulum structure during mouse oocyte maturation are controlled by the cytoskeleton and cytoplasmic dynein.

    abstract::Oocyte maturation in mouse is associated with a dramatic reorganisation of the endoplasmic reticulum (ER) from a network of cytoplasmic accumulations in the germinal vesicle-stage oocyte (GV) to a network of distinctive cortical clusters in the metaphase II egg (MII). Multiple lines of evidence suggest that this redis...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: FitzHarris G,Marangos P,Carroll J

    更新日期:2007-05-01 00:00:00

  • The basal position of nuclei is one pre-requisite for asymmetric cell divisions in the early mouse embryo.

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    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Ajduk A,Biswas Shivhare S,Zernicka-Goetz M

    更新日期:2014-08-15 00:00:00

  • Cytoplasmic aggregation of DDX1 in developing embryos: Early embryonic lethality associated with Ddx1 knockout.

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    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Hildebrandt MR,Wang Y,Li L,Yasmin L,Glubrecht DD,Godbout R

    更新日期:2019-11-15 00:00:00

  • Analysis of transcriptional codes for zebrafish dopaminergic neurons reveals essential functions of Arx and Isl1 in prethalamic dopaminergic neuron development.

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    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Filippi A,Jainok C,Driever W

    更新日期:2012-09-01 00:00:00

  • Desert hedgehog-primary cilia cross talk shapes mitral valve tissue by organizing smooth muscle actin.

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    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Fulmer D,Toomer KA,Glover J,Guo L,Moore K,Moore R,Stairley R,Gensemer C,Abrol S,Rumph MK,Emetu F,Lipschutz JH,McDowell C,Bian J,Wang C,Beck T,Wessels A,Renault MA,Norris RA

    更新日期:2020-07-01 00:00:00

  • The regulatory mechanisms that underlie inappropriate transcription of the myogenic determination gene Myf5 in the central nervous system.

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    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Daubas P,Crist CG,Bajard L,Relaix F,Pecnard E,Rocancourt D,Buckingham M

    更新日期:2009-03-01 00:00:00

  • Production and utilization of growth factors related to fibroblast growth factor by embryonal carcinoma cells and their differentiated cells.

    abstract::Previous studies have established that embryonal carcinoma (EC) cells produce several different growth factors, but express few, if any, receptors for epidermal growth factor, platelet-derived growth factor, or transforming growth factor type-beta. In this study, the production and utilization of fibroblast growth fac...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Rizzino A,Kuszynski C,Ruff E,Tiesman J

    更新日期:1988-09-01 00:00:00

  • Involvement of an inhibitory G-protein in the signal transduction pathway of maturation-inducing hormone (17 alpha,20 beta-dihydroxy-4-pregnen-3-one) action in rainbow trout (Oncorhynchus mykiss) oocytes.

    abstract::To investigate the mechanism by which the hormone 17 alpha,20 beta-dihydroxy-4-pregnen-3-one(17 alpha,20 beta-DP) acts on a receptor on the external surface of rainbow trout oocytes to induce maturation, the interaction between 17 alpha,20 beta-DP receptors and G-proteins was examined. Pertussis toxin (PT) catalyzed t...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Yoshikuni M,Nagahama Y

    更新日期:1994-12-01 00:00:00

  • Regulation of nodal and BMP signaling by tomoregulin-1 (X7365) through novel mechanisms.

    abstract::During early vertebrate development, members of the transforming growth factor beta (TGFbeta) family play important roles in a variety of processes, including germ layer specification, patterning, cell differentiation, migration, and organogenesis. The activities of TGFbetas need to be tightly controlled to ensure the...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Chang C,Eggen BJ,Weinstein DC,Brivanlou AH

    更新日期:2003-03-01 00:00:00

  • Axonal pathfinding in organ-cultured embryonic avian retinae.

    abstract::Eye cups from stage 14-28 (E2 to E5) chick and quail embryos consisting of neural retina, lens, and vitreous body were cultured for 1 or 2 days. These eyes expanded by proliferation of the retinal cells and the surface areas of the retinae increased several-fold. The area covered by ganglion cells and axons also expan...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Halfter W,Deiss S

    更新日期:1986-04-01 00:00:00

  • Gap junction signalling mediated through connexin-43 is required for chick limb development.

    abstract::During chick limb development the gap junction protein Connexin-43 (Cx43) is expressed in discrete spatially restricted domains in the apical ectodermal ridge (AER) and mesenchyme of the zone of polarising activity. Antisense oligonucleotides (ODNs) were used to investigate the role of Connexin-43 (Cx43) in the develo...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Makarenkova H,Patel K

    更新日期:1999-03-15 00:00:00

  • Levels of the ubiquitin ligase substrate adaptor MEL-26 are inversely correlated with MEI-1/katanin microtubule-severing activity during both meiosis and mitosis.

    abstract::The MEI-1/MEI-2 microtubule-severing complex, katanin, is required for oocyte meiotic spindle formation and function in C. elegans, but the microtubule-severing activity must be quickly downregulated so that it does not interfere with formation of the first mitotic spindle. Post-meiotic MEI-1 inactivation is accomplis...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Johnson JL,Lu C,Raharjo E,McNally K,McNally FJ,Mains PE

    更新日期:2009-06-15 00:00:00

  • Tie1 regulates zebrafish cardiac morphogenesis through Tolloid-like 1 expression.

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    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Carlantoni C,Allanki S,Kontarakis Z,Rossi A,Piesker J,Günther S,Stainier DYR

    更新日期:2021-01-01 00:00:00

  • Altered proteoglycan synthesis disrupts feather pattern formation in chick embryonic skin.

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    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Goetinck PF,Carlone DL

    更新日期:1988-05-01 00:00:00

  • CnNK-2, an NK-2 homeobox gene, has a role in patterning the basal end of the axis in hydra.

    abstract::NK-2-class homeobox genes have been identified in a variety of metazoans, from sponges to arthropods and vertebrates, and have been shown to play roles in a variety of cell and tissue specifications. Here we describe the characterization of the NK-2 homolog CnNK-2 from Hydra vulgaris, a freshwater cnidarian. CnNK-2 ex...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Grens A,Gee L,Fisher DA,Bode HR

    更新日期:1996-12-15 00:00:00

  • The Drosophila LEM-domain protein MAN1 antagonizes BMP signaling at the neuromuscular junction and the wing crossveins.

    abstract::BMP signaling responses are refined by distinct secreted and intracellular antagonists in different cellular and temporal contexts. Here, we show that the nuclear LEM-domain protein MAN1 is a tissue-specific antagonist of BMP signaling in Drosophila. MAN1 contains two potential Mad-binding sites. We generated MAN1Delt...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Wagner N,Weyhersmüller A,Blauth A,Schuhmann T,Heckmann M,Krohne G,Samakovlis C

    更新日期:2010-03-01 00:00:00

  • Molecular cloning of the lethal(1)discs large-1 oncogene of Drosophila.

    abstract::We present a genetic, developmental and molecular analysis of lethal(1)discs large-1[l(1)d.lg-1; Stewart et al., 1972], an oncogene of Drosophila. Mutations in this gene cause the imaginal discs to grow by cell proliferation beyond their normal final size, transform into solid tumors, fuse with one another and the bra...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Woods DF,Bryant PJ

    更新日期:1989-07-01 00:00:00

  • Hox C cluster genes are dispensable for overall body plan of mouse embryonic development.

    abstract::Hox genes encode transcription factors which provide positional information during morphogenesis along the body axis; genetic interaction among Hox genes is thought to be necessary for correct pattern formation. One of the most curious features of the 39 vertebrate Hox genes is that they form four clusters each compos...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Suemori H,Noguchi S

    更新日期:2000-04-15 00:00:00

  • Localization and function of Bam protein require the benign gonial cell neoplasm gene product.

    abstract::Division of a female Drosophila stem cell produces a daughter stem cell and a cystoblast. The cystoblast produces a syncytial cluster of 16 cells by precisely four mitotic divisions and incomplete cytokinesis. Mutations in genes required for cystoblast differentiation, such as bag-of-marbles, block syncytial cluster f...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Lavoie CA,Ohlstein B,McKearin DM

    更新日期:1999-08-15 00:00:00

  • Characterization and localization of a mouse egg cortical granule antigen prior to and following fertilization or egg activation.

    abstract::Immunological approaches were used to characterize an antigen that is present within the cortical granules of mouse oocytes and eggs. Immunoelectron microscopy shows a specific localization of the antigen to the cortical granules in the cortex of mouse oocytes and eggs. Following in vitro fertilization, the antigen is...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Pierce KE,Siebert MC,Kopf GS,Schultz RM,Calarco PG

    更新日期:1990-10-01 00:00:00

  • MEMO1 drives cranial endochondral ossification and palatogenesis.

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    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Van Otterloo E,Feng W,Jones KL,Hynes NE,Clouthier DE,Niswander L,Williams T

    更新日期:2016-07-15 00:00:00

  • Differential effect of activators of protein kinase C on cytoskeletal changes in mouse and hamster eggs.

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    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Moore GD,Kopf GS,Schultz RM

    更新日期:1995-08-01 00:00:00

  • Radial leaves of the maize mutant ragged seedling2 retain dorsiventral anatomy.

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    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Henderson DC,Muehlbauer GJ,Scanlon MJ

    更新日期:2005-06-15 00:00:00

  • Protein kinase A regulates GDNF/RET-dependent but not GDNF/Ret-independent ureteric bud outgrowth from the Wolffian duct.

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    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Tee JB,Choi Y,Shah MM,Dnyanmote A,Sweeney DE,Gallegos TF,Johkura K,Ito C,Bush KT,Nigam SK

    更新日期:2010-11-15 00:00:00

  • Na/K-ATPase-mediated 86Rb+ uptake and asymmetrical trophectoderm localization of alpha1 and alpha3 Na/K-ATPase isoforms during bovine preattachment development.

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    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Betts DH,Barcroft LC,Watson AJ

    更新日期:1998-05-01 00:00:00

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    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Palade J,Djordjevic D,Hutchins ED,George RM,Cornelius JA,Rawls A,Ho JWK,Kusumi K,Wilson-Rawls J

    更新日期:2018-01-15 00:00:00

  • Dissociation and sorting out of Drosophila imaginal disc cells.

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    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Fehon RG,Schubiger G

    更新日期:1985-04-01 00:00:00

  • Sprouty gain of function disrupts lens cellular processes and growth by restricting RTK signaling.

    abstract::Sprouty proteins function as negative regulators of the receptor tyrosine kinase (RTK)-mediated Ras/Raf/MAPK pathway in many varied physiological and developmental processes, inhibiting growth factor-induced cellular proliferation, migration and differentiation. Like other negative regulators, Sprouty proteins are exp...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Shin EH,Zhao G,Wang Q,Lovicu FJ

    更新日期:2015-10-15 00:00:00

  • E-cadherin is required for cranial neural crest migration in Xenopus laevis.

    abstract::The cranial neural crest (CNC) is a highly motile and multipotent embryonic cell population, which migrates directionally on defined routes throughout the embryo, contributing to facial structures including cartilage, bone and ganglia. Cadherin-mediated cell-cell adhesion is known to play a crucial role in the directi...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Huang C,Kratzer MC,Wedlich D,Kashef J

    更新日期:2016-03-15 00:00:00