Intracellular pH regulation in chicken enterocytes: the importance of extracellular pH.

Abstract:

:The present work reports the effect of pHo on pHi and Na(+)-H+ exchanger activity. Intracellular pH tended to follow pHo, but the proton distribution across the cell membrane is not at electrochemical equilibrium. Removal of external Na+ acidified the cells by both reversing the direction of the Na(+)-H+ exchanger and hyperpolarizing the cell membrane potential. The relationship between pHo and the rate of Na(+)-dependent proton efflux following an acid load suggests that external protons interact with the Na(+)-H+ exchanger at a single site with an apparent pK (-log of the dissociation constant) of 7.22. The results demonstrate that maintenance of pHo in the physiological range is essential for maintenance of normal cell pH and that the activity of the Na(+)-H+ exchanger involved in pHi regulation is affected by external protons. The results also suggest that, at least at low pHo, some intracellular mechanism is involved in pHi regulation.

journal_name

Exp Physiol

journal_title

Experimental physiology

authors

Peral MJ,Calonge ML,Ilundáin AA

doi

10.1113/expphysiol.1995.sp003897

subject

Has Abstract

pub_date

1995-11-01 00:00:00

pages

1001-7

issue

6

eissn

0958-0670

issn

1469-445X

journal_volume

80

pub_type

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