Abstract:
:The present work reports the effect of pHo on pHi and Na(+)-H+ exchanger activity. Intracellular pH tended to follow pHo, but the proton distribution across the cell membrane is not at electrochemical equilibrium. Removal of external Na+ acidified the cells by both reversing the direction of the Na(+)-H+ exchanger and hyperpolarizing the cell membrane potential. The relationship between pHo and the rate of Na(+)-dependent proton efflux following an acid load suggests that external protons interact with the Na(+)-H+ exchanger at a single site with an apparent pK (-log of the dissociation constant) of 7.22. The results demonstrate that maintenance of pHo in the physiological range is essential for maintenance of normal cell pH and that the activity of the Na(+)-H+ exchanger involved in pHi regulation is affected by external protons. The results also suggest that, at least at low pHo, some intracellular mechanism is involved in pHi regulation.
journal_name
Exp Physioljournal_title
Experimental physiologyauthors
Peral MJ,Calonge ML,Ilundáin AAdoi
10.1113/expphysiol.1995.sp003897subject
Has Abstractpub_date
1995-11-01 00:00:00pages
1001-7issue
6eissn
0958-0670issn
1469-445Xjournal_volume
80pub_type
杂志文章abstract::Cardiovascular arousal is associated with patterned cortical activity changes. Head-down-tilt bed rest (HDBR) dimishes the baroreflex-mediated cardiac control. The present study tested the hypothesis that HDBR deconditioning would modify the forebrain organization for heart rate (HR) control during baroreflex unloadin...
journal_title:Experimental physiology
pub_type: 杂志文章,随机对照试验
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更新日期:2003-05-01 00:00:00
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更新日期:2010-11-01 00:00:00
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