Mitochondrial Ca2+ transport in frog early distal tubule.

Abstract:

:A global and transient rise of intracellular Ca2+ (Ca2+i) is central to the operation of pump-leak coupling in the frog early distal tubule (EDT). The endoplasmic reticulum (ER) is the site of this Ca2+ release and reuptake; however, it is likely that other intracellular pools, such as mitochondria, also contribute to cellular Ca2+ homeostasis. The present study was performed to seek evidence of mitochondrial Ca2+ transport in the frog EDT. Experiments were performed on isolated and permeabilized EDT segments from the frog kidney loaded with the low-affinity, Ca2+-sensitive fluorescent indicator, mag-fura-2. Ca2+ uptake in the absence of SarcoEndoplasmic Reticulum Calcium ATPase (SERCA) activity (inhibition by 2,5-di-t-butyl hydroquinone, TBQ) was evident at a bath [Ca2+] of 1 microm, but not at 200 nm, in the presence of ATP. This uptake was sensitive to the protonophore FCCP and the ATP-synthase inhibitor oligomycin. Ca2+ uptake was also stimulated by respiratory substrates; this uptake was enhanced by oligomycin and reversed by the application of FCCP. These findings provide the first evidence of mitochondrial Ca2+ transport in renal tubules, which appears to occur via a low-affinity pathway and which will act as a physiological Ca2+ buffer, protecting the cell from large increases in Ca2+i.

journal_name

Exp Physiol

journal_title

Experimental physiology

authors

Fowler MR,Hunter M

doi

10.1113/expphysiol.2004.028886

subject

Has Abstract

pub_date

2005-03-01 00:00:00

pages

195-201

issue

2

eissn

0958-0670

issn

1469-445X

pii

expphysiol.2004.028886

journal_volume

90

pub_type

杂志文章
  • Modulation of voltage-dependent calcium current in Helix aspersa buccal neurones by serotonin and protein kinase C activators.

    abstract::In Helix aspersa, activation of the cerebral giant serotonin neurones (GSNs) evokes a biphasic, excitatory synaptic response in the M neurones of the buccal ganglia. Local application of serotonin to the current-clamped M neurones also evokes fast and slow depolarizing responses. The slow response is thought to be dep...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1992.sp003656

    authors: Hill-Venning C,Cottrell GA

    更新日期:1992-11-01 00:00:00

  • Organ-specific ligation-induced changes in harmonic components of the pulse spectrum and regional vasoconstrictor selectivity in Wistar rats.

    abstract::It has been shown previously that the amplitudes of the harmonic components of the pulse spectrum vary in specific patterns when the arteries leading to different organs are ligated, with the variations in the harmonics being linearly additive. Since ligation can be regarded as a vast increase in organ resistance, the...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.2005.031575

    authors: Hsu TL,Chao PT,Hsiu H,Wang WK,Li SP,Wang YY

    更新日期:2006-01-01 00:00:00

  • Reduced hypoxic ventilatory response in newborn mice knocked-out for the progesterone receptor.

    abstract::Recent studies showed that progesterone stimulates the hypoxic ventilatory response and may reduce apnoea frequency in newborn rats, but so far we still do not know by what mechanisms and whether endogenous progesterone might contribute to respiratory control in neonates. We therefore determined the role of the nuclea...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.2014.080986

    authors: Potvin C,Rossignol O,Uppari N,Dallongeville A,Bairam A,Joseph V

    更新日期:2014-11-01 00:00:00

  • Dietary salt-loading attenuates endothelium-dependent relaxation in response to histamine but not to acetylcholine in rat aortic rings.

    abstract::Endothelium-dependent relaxation in response to histamine and ACh has been studied on precontracted aortic rings from control and salt-loaded Sprague-Dawley rats. Both ACh and histamine caused relaxation of the noradrenaline-induced precontraction only in the presence of the endothelium. The relaxation response to ACh...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1991.sp003475

    authors: Obiefuna PC,Sofola OA,Ebeigbe AB

    更新日期:1991-01-01 00:00:00

  • Effect of short-term training on GLUT-4 mRNA and protein expression in human skeletal muscle.

    abstract::Six untrained, male subjects (23 +/- 1 years old, 84 +/- 5 kg, (O(2)peak)= 3.7 +/- 0.8 l min(-1)) exercised for 60 min at 75 +/- 1%(O(2)peak) on 7 consecutive days. Muscle samples were obtained before the start of cycle exercise training and 24 h after the first and seventh exercise sessions and analysed for citrate s...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.2004.027409

    authors: Kraniou GN,Cameron-Smith D,Hargreaves M

    更新日期:2004-09-01 00:00:00

  • Effect of body posture on postexercise parasympathetic reactivation in men.

    abstract::The aim of the present study was to assess the influence of body posture on post-submaximal exercise parasympathetic reactivation and to examine whether this influence was preserved under a heightened sympathetic background. On four occasions, eleven moderately trained subjects (22.1 +/- 3.0 years old) performed, in r...

    journal_title:Experimental physiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1113/expphysiol.2009.048041

    authors: Buchheit M,Al Haddad H,Laursen PB,Ahmaidi S

    更新日期:2009-07-01 00:00:00

  • Long-term administration of L-arginine did not influence blood pressure, heart rate, cardiac hypertrophy or arterial wall thickness of spontaneously hypertensive rats.

    abstract::The aim of this study was to evaluate whether long-term administration of L-arginine, a physiological substrate for the production of nitric oxide, improved blood pressure, heart rate, cardiac hypertrophy and particularly structural changes in the coronary and carotid artery of spontaneously hypertensive rats (SHR). T...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1998.sp004141

    authors: Kristek F

    更新日期:1998-09-01 00:00:00

  • Oestradiol affects skeletal muscle mass, strength and satellite cells following repeated injuries.

    abstract:NEW FINDINGS:What is the central question of this study? Oestradiol (E2 ) plays an important role in regulating skeletal muscle strength in females. To what extent does E2 deficiency affect recovery of strength and satellite cell number when muscle is challenged by multiple injuries? What is the main finding and its im...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/EP088827

    authors: Larson AA,Baumann CW,Kyba M,Lowe DA

    更新日期:2020-10-01 00:00:00

  • Dynamic characteristics of heart rate control by the autonomic nervous system in rats.

    abstract::We estimated the transfer function of autonomic heart rate (HR) control by using random binary sympathetic or vagal nerve stimulation in anaesthetized rats. The transfer function from sympathetic stimulation to HR response approximated a second-order, low-pass filter with a lag time (gain, 4.29 +/- 1.55 beats min(1) H...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.2010.053090

    authors: Mizuno M,Kawada T,Kamiya A,Miyamoto T,Shimizu S,Shishido T,Smith SA,Sugimachi M

    更新日期:2010-09-01 00:00:00

  • Acute and chronic effects of pancreatic glucagon on sugar transport across the brush-border and basolateral membranes of rat jejunal enterocytes.

    abstract::Preparations of isolated rat jejunal enterocyte and brush-border and basolateral membrane vesicles have been used to study the effects of a 15 min exposure of upper and mid-villus enterocytes to pancreatic glucagon on the initial, unidirectional phlorhizin-sensitive (brush border) transport of galactose and phlorhizin...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1993.sp003680

    authors: Debnam ES,Sharp PA

    更新日期:1993-03-01 00:00:00

  • Volumetric change of the lateral ventricles in the human brain following glucose loading.

    abstract::Lateral ventricular volumes were monitored and quantified using accurately registered magnetic resonance images (MRIs) in six healthy individuals 30 min before and up to 4 h after ingestion of a glucose drink. The volume of the lateral ventricles increased by an average (+/- S.E.M.) of 2.4 +/- 0.4% as blood glucose le...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1111/j.1469-445x.1999.tb00085.x

    authors: Puri BK,Lewis HJ,Saeed N,Davey NJ

    更新日期:1999-01-01 00:00:00

  • On the role of bicarbonate as a hydrogen ion buffer in rat CNS neurones.

    abstract::The role of bicarbonate as a hydrogen ion buffer has been investigated using the fluorescent dye BCECF in individual rat cerebellar, hippocampal and neocortical neurones maintained in culture. The steady-state intracellular pH (pHi) was estimated to be 7.07 +/- 0.05 (n = 22) in CO2-HCO3(-)-buffered media. Buffering po...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1996.sp003963

    authors: Amos BJ,Pocock G,Richards CD

    更新日期:1996-07-01 00:00:00

  • Neopterin/7,8-dihydroneopterin is elevated in Duchenne muscular dystrophy patients and protects mdx skeletal muscle function.

    abstract:NEW FINDINGS:What is the central question of this study? We examined whether the macrophage-synthesized antioxidant 7,8-dihydroneopterin was elevated in Duchenne muscular dystrophy (DMD) patients. We then examined whether 7,8-dihydroneopterin could protect dystrophic skeletal mouse muscle from eccentric contraction-ind...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/EP087031

    authors: Lindsay A,Schmiechen A,Chamberlain CM,Ervasti JM,Lowe DA

    更新日期:2018-07-01 00:00:00

  • Cerebral haemodynamics and oxygenation during whole-body exercise over 5 days at high altitude.

    abstract:NEW FINDINGS:What is the central question of this study? Impairment and subsequent improvement in cerebral oxygenation during acute and prolonged exposure to high altitude affect exercise performance. This study innovates by investigating the effect of acute and prolonged high-altitude exposure on cerebral haemodynamic...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/EP088354

    authors: Marillier M,Rupp T,Bouzat P,Walther G,Baillieul S,Millet GY,Robach P,Verges S

    更新日期:2021-01-01 00:00:00

  • The glucogenic capacity of the fetal pig: developmental regulation by cortisol.

    abstract::In the present study, the ontogenic changes in gluconeogenic enzyme activities and in hepatic glycogen and beta-adrenergic receptor levels were investigated in fetal pigs from 70 days of gestation until delivery at term (114 +/- 2 days). The values were compared with those observed in fetuses infused subcutaneously wi...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1995.sp003860

    authors: Fowden AL,Apatu RS,Silver M

    更新日期:1995-05-01 00:00:00

  • Eccentric cardiac hypertrophy was induced by long-term intermittent hypoxia in rats.

    abstract::It is unclear whether cardiac hypertrophy and hypertrophy-related pathways will be induced by long-term intermittent hypoxia. Thirty-six Sprague-Dawley rats were randomly assigned into three groups: normoxia, and long-term intermittent hypoxia (12% O(2), 8 h per day) for 4 weeks (4WLTIH) or for 8 weeks (8WLTIH). Myoca...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.2006.036590

    authors: Chen LM,Kuo WW,Yang JJ,Wang SG,Yeh YL,Tsai FJ,Ho YJ,Chang MH,Huang CY,Lee SD

    更新日期:2007-03-01 00:00:00

  • Age and sex differences in muscle sympathetic nerve activity in relation to haemodynamics, blood volume and left ventricular size.

    abstract::We compared the effect of age- and sex-related differences in haemodynamics, blood volume (BV) and left ventricular (LV) size and mass on resting muscle sympathetic nerve activity (MSNA) in healthy, normotensive adults. Twenty young men (19-47 years old) and 20 young women (21-46 years old) as well as 15 older men (62...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.2013.077248

    authors: Best SA,Okada Y,Galbreath MM,Jarvis SS,Bivens TB,Adams-Huet B,Fu Q

    更新日期:2014-06-01 00:00:00

  • Human amniotic membrane mesenchymal stem cell-conditioned medium reduces inflammatory factors and fibrosis in ovalbumin-induced asthma in mice.

    abstract:NEW FINDINGS:What is the central question of this study? Is mesenchymal stem cell-conditioned medium capable of improving the pathological alterations of ovalbumin-induced asthma in mice? What is the main finding and its importance? Our study indicated that human amniotic membrane mesenchymal stem cell-conditioned medi...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/EP088911

    authors: Dalouchi F,Falak R,Bakhshesh M,Sharifiaghdam Z,Azizi Y,Aboutaleb N

    更新日期:2020-12-01 00:00:00

  • Influence of muscle fibre type and fitness on the oxygen uptake/power output slope during incremental exercise in humans.

    abstract::We recently reported that a higher percentage of type I fibres in vastus lateralis and a greater peak oxygen uptake (O2) were associated with a greater initial rise in O2 (O2 /W, where W is work rate) following the onset of heavy constant power output exercise (above the lactate threshold, LT). It was unclear if these...

    journal_title:Experimental physiology

    pub_type: 临床试验,杂志文章

    doi:

    authors: Barstow TJ,Jones AM,Nguyen PH,Casaburi R

    更新日期:2000-01-01 00:00:00

  • Inhibition of augmented muscle vasoconstrictor drive following asphyxic apnoea in awake human subjects is not affected by relief of chemical drive.

    abstract::Progressive asphyxia, produced by a prolonged voluntary breath hold (end-expiratory apnoea), evokes large bursts of muscle sympathetic nerve activity (MSNA). These bursts increase in amplitude until the asphyxic break point is reached, at which point the bursts are inhibited. We tested the hypothesis that lung inflati...

    journal_title:Experimental physiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1113/expphysiol.2012.067421

    authors: Seitz MJ,Brown R,Macefield VG

    更新日期:2013-02-01 00:00:00

  • Effects of sex and ovarian hormones on the initial renal sympathetic nerve activity response to myocardial infarction.

    abstract::The physiological mechanisms contributing to sex differences following myocardial infarction (MI) are poorly understood. Given the strong relationship between sympathetic nerve activity (SNA) and outcome, we hypothesized there may be a sex difference in SNA responses to MI. In anaesthetized, open-chest male, female an...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.2012.065615

    authors: Pinkham MI,Guild SJ,Malpas SC,Barrett CJ

    更新日期:2012-09-01 00:00:00

  • Electrical pulse stimulation: an in vitro exercise model for the induction of human skeletal muscle cell hypertrophy. A proof-of-concept study.

    abstract:NEW FINDINGS:What is the central question of this study? Is electrical pulse stimulation (EPS) an in vitro exercise model able to elicit the hypertrophy of human muscle cells? What is the main finding and its importance? The addition of a restitution period of 8 h after EPS induces the enlargement of human muscle cells...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/EP086581

    authors: Tarum J,Folkesson M,Atherton PJ,Kadi F

    更新日期:2017-11-01 00:00:00

  • Confocal analysis of fluorescent bead uptake by mouse Peyer's patch follicle-associated M cells.

    abstract::Latex beads coated with secretory immunoglobulin (IgA) instilled into mouse intestinal loops are taken up by M cells present in Peyer's patch follicle-associated epithelial tissue. Bead adsorption and uptake is greater at the edge compared with the apex of follicle domes. Coating beads with bovine serum albumin (BSA) ...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1992.sp003662

    authors: Porta C,James PS,Phillips AD,Savidge TC,Smith MW,Cremaschi D

    更新日期:1992-11-01 00:00:00

  • Chronic intermittent hypoxia and acetaminophen induce synergistic liver injury in mice.

    abstract::Obstructive sleep apnoea (OSA) leads to chronic intermittent hypoxia (CIH) during sleep. Obstructive sleep apnoea has been associated with liver injury. Acetaminophen (APAP; known as paracetamol outside the USA) is one of the most commonly used drugs which has known hepatotoxicity. The goal of the present study was to...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.2008.044883

    authors: Savransky V,Reinke C,Jun J,Bevans-Fonti S,Nanayakkara A,Li J,Myers AC,Torbenson MS,Polotsky VY

    更新日期:2009-02-01 00:00:00

  • Acute systemic hypoxia and the surface ultrastructure and morphological characteristics of rat leucocytes.

    abstract::We have examined the surface ultrastructure and morphological characteristics of leucocytes obtained from control rats breathing air and rats made acutely hypoxic (breathing 6% O2, for 30 min). Striking ultrastructural and morphological changes occurred in the leucocytes of hypoxic rats. These changes included the app...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1993.sp003731

    authors: Mian R,Westwood D,Stanley P,Marshall JM,Coote JH

    更新日期:1993-11-01 00:00:00

  • Do cardiac neurons play a role in the intrinsic control of heart rate in the rat?

    abstract::Three experiments were performed to see whether cardiac neurons contribute to the intrinsic control of heart rate in right atria of adult rats. The intrinsic heart rate response (IRR) was examined by raising right atrial pressure from 2 to 8 mmHg for 3 min. In isolated preparations of the right atrium, the IRR was not...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/eph8702364

    authors: Wilson SJ,Bolter CP

    更新日期:2002-11-01 00:00:00

  • Mechanism of pregnancy-induced attenuation of contraction to phenylephrine in rat aorta.

    abstract::The role of endothelium in the decreased contraction to phenylephrine in aorta of pregnant Wistar rats has been studied. Contractions in response to phenylephrine were significantly decreased in aortic rings from pregnant rats compared to those from non-pregnant rats. Pretreatment of the rings with indomethacin did no...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.1993.sp003694

    authors: Aloamaka CP,Ezimokhai M,Cherian T,Morrison J

    更新日期:1993-05-01 00:00:00

  • Response of skeletal muscle mitochondria to hypoxia.

    abstract::This review explores the current concepts relating the structural and functional modifications of skeletal muscle mitochondria to the molecular mechanisms activated when organisms are exposed to a hypoxic environment. In contrast to earlier assumptions it is now established that permanent or long-term exposure to seve...

    journal_title:Experimental physiology

    pub_type: 杂志文章,评审

    doi:10.1113/eph8802513

    authors: Hoppeler H,Vogt M,Weibel ER,Flück M

    更新日期:2003-01-01 00:00:00

  • Muscle contraction duration and fibre recruitment influence blood flow and oxygen consumption independent of contractile work during steady-state exercise in humans.

    abstract::We tested the hypothesis that, among conditions of matched contractile work, shorter contraction durations and greater muscle fibre recruitment result in augmented skeletal muscle blood flow and oxygen consumption ( ) during steady-state exercise in humans. To do so, we measured forearm blood flow (FBF; Doppler ultras...

    journal_title:Experimental physiology

    pub_type: 杂志文章

    doi:10.1113/expphysiol.2011.062968

    authors: Richards JC,Crecelius AR,Kirby BS,Larson DG,Dinenno FA

    更新日期:2012-06-01 00:00:00

  • Effect of whole body resistance training on arterial compliance in young men.

    abstract::The effect of resistance training on arterial stiffening is controversial. We tested the hypothesis that resistance training would not alter central arterial compliance. Young healthy men (age, 23 +/- 3.9 (mean +/- s.e.m.) years; n = 28,) were whole-body resistance trained five times a week for 12 weeks, using a rotat...

    journal_title:Experimental physiology

    pub_type: 临床试验,杂志文章

    doi:10.1113/expphysiol.2004.029504

    authors: Rakobowchuk M,McGowan CL,de Groot PC,Bruinsma D,Hartman JW,Phillips SM,MacDonald MJ

    更新日期:2005-07-01 00:00:00