Abstract:
:Induction of mutations to 6-thioguanine resistance (TGr) by gamma-rays at three different dose-rates and molecular changes in the HPRT gene were studied in human lymphoblastoid WIL2-NS cells. Mutant induction showed a curvilinear dose-response for acute irradiation (30 Gy/h). The total mutant frequency was lower after irradiation at 0.17 or 0.006 Gy/h compared with acute irradiation. An apparent linear relationship between total dose and mutant frequency was found for the chronic irradiations. Spontaneous mutant frequency increased linearly with the exposure time of protracted irradiation at 0.006 Gy/h. After the spontaneous mutant frequency was subtracted from the total mutant frequency for irradiation at 0.006 Gy/h, no significant difference was found in the mutant frequency as a function of dose between the cultures irradiated at 0.17 Gy/h and those at 0.006 Gy/h. The inverse dose-rate effect, which has been observed in proliferating mouse L5178Y leukemia cells was not evident in WIL2-NS cells at the dose-rates employed. Structural alterations at the HPRT locus in TGr mutants were examined with the multiplex PCR method and compared among cultures irradiated at different dose-rates. Assuming that the mutants isolated were primarily independent, approximately 17% of spontaneous mutants were deletion mutants. When the fraction of spontaneous mutants in the irradiated cultures was subtracted from the total fraction of each type of mutant, it is clear that low dose-rate gamma-rays induced deletion mutations at the HPRT locus just as efficiently (79%) as high dose-rate gamma-rays (74%).
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Furuno-Fukushi I,Tatsumi K,Takahagi M,Tachibana Adoi
10.1080/095530096145201subject
Has Abstractpub_date
1996-08-01 00:00:00pages
209-17issue
2eissn
0955-3002issn
1362-3095journal_volume
70pub_type
杂志文章abstract::In this report, in situ hybridization with whole chromosome painting probes was used to paint radiation-induced micronuclei (MN) in three lymphoblastoid cells lines to investigate the frequency of radiation-induced MN. The results obtained for four different chromosomes showed that there was a significant deviation of...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530096144860
更新日期:1996-10-01 00:00:00
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journal_title:International journal of radiation biology
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更新日期:1993-05-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009414551111
更新日期:1994-08-01 00:00:00
abstract:PURPOSE:To investigate whether mobile phone radiation might affect snail nociception, employing radiofrequency (RF) electromagnetic fields (EMF) which, to our knowledge, have hitherto not been studied in a snail model. Exposure to extremely low frequency (ELF) magnetic fields has however been shown to significantly aff...
journal_title:International journal of radiation biology
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doi:10.3109/09553002.2012.644257
更新日期:2012-03-01 00:00:00
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更新日期:2006-11-01 00:00:00
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更新日期:2008-06-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008914550841
更新日期:1989-05-01 00:00:00
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更新日期:2011-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1080/09553009214551981
更新日期:1992-08-01 00:00:00
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doi:10.1080/09553009314450831
更新日期:1993-05-01 00:00:00
abstract:PURPOSE:The hemorrhagic syndrome is a major cause of morbidity and mortality associated with the acute radiation syndrome (ARS). We previously characterized the dose-response relationship for total body irradiation (TBI)-induced ARS in the New Zealand White (NZW) rabbit. Thrombocytopenia, hemorrhage, and anemia were st...
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abstract::Radiation-induced DNA double-strand breaks (dsbs) were measured in CHO-K1 cells by means of an experimental approach involving constant-field gel electrophoresis and densitometric scanning of ethidium bromide stained gels. For X-irradiation, an induction efficiency of 36 +/- 5 dsbs (Gy x cell)-1 was determined. With t...
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pub_type: 杂志文章
doi:10.1080/09553000050151592
更新日期:2000-10-01 00:00:00
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doi:
更新日期:2003-01-01 00:00:00
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更新日期:2013-11-01 00:00:00
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更新日期:2004-08-01 00:00:00
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更新日期:2016-10-01 00:00:00
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journal_title:International journal of radiation biology
pub_type: 杂志文章
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更新日期:2017-07-01 00:00:00
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更新日期:2003-10-01 00:00:00