当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF RADIATION BIOLOGY期刊下所有文献 > Low-dose hyper-radiosensitivity in human hepatocellular HepG2 cells is associated with Cdc25C-mediated G2/M cell cycle checkpoint control.

Low-dose hyper-radiosensitivity in human hepatocellular HepG2 cells is associated with Cdc25C-mediated G2/M cell cycle checkpoint control.

Abstract：

PURPOSE:Although the significance of cell cycle checkpoints in overcoming low-dose hyper-radiosensitivity (HRS) has been proposed, the underlying mechanism of HRS in human hepatocellular cells remains unclear. Therefore, the aim of this study was to characterize HRS inhuman hepatocellular HepG2 cells and to explore the molecular mechanism(s) mediating this response. MATERIALS AND METHODS:HepG2 cells were exposed to various single doses of γ radiation (from 0 Gy to 4 Gy), and then were assayed at subsequent time-points. Survival curves were then generated using a linear-quadratic (LQ) equation and a modified induced repair model (MIRM). The percentage of cells in the G1, G2/M, and S phases of the cell cycle were also examined using propidium iodide (PI) staining and flow cytometry. Levels of total cell division cyclin 25C (Cdc25C) and phosphorylated Cdc25C were examined by Western blotting. RESULTS:Low-dose γ radiation (<0.3 Gy) induced HRS in HepG2 cells, while doses of 0.3, 0.5, and 2.0 Gy γ radiation significantly arrested HepG2 cells in the G2/M phase. While total Cdc25C levels remained unchanged after irradiation, levels of phosphorylated Cdc25C markedly increased 6, 16, and 24 h after treatment with 0.5 or 2.0 Gy radiation, and they peaked after 16 h. The latter observation is consistent with the G2/M arrest that was detected following irradiation. CONCLUSIONS:These findings indicate that low-dose HRS in HepG2 cells may be associated with Cdc25C-mediated G2/M cell cycle checkpoint control.

journal_name

Int J Radiat Biol

journal_title

International journal of radiation biology

authors

Xue J,Zong Y,Li PD,Wang LX,Li YQ,Niu YF

doi

10.1080/09553002.2016.1206235

subject

Has Abstract

pub_date

2016-10-01 00:00:00

pages

543-7

issue

10

eissn

0955-3002

issn

1362-3095

journal_volume

92

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY文献大全
  • Relative toxicity of (45)Ca beta-particles and (242)Cm alpha-particles following their intravenous injection into mice as radiolabelled FAP.

    abstract：PURPOSE:To determine the relative toxicity of alpha- and beta-radiations under conditions of controlled temporal and spatial dose distribution. METHODS:Fused aluminosilicate particles were radiolabelled with either (45)Ca (a beta-emitter) or (242)Cm (an alpha-emitter). These were injected into CBA/Ca mice to give life...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.3109/09553000903564075

    authors： Priest ND,Hoel DG,Brooks PN

    更新日期：2010-04-01 00:00:00

  • Chromosomal aberration types in cells at the second division after irradiation in G1 or G2.

    abstract：:Cultures of JU-56 cells were irradiated in either G1 or G2 and examined either in their first post-irradiation metaphase (in diploids) or in their second post-irradiation metaphase (in colcemid-induced tetraploids). The timing of fixation, together with tritiated thymidine pulse labelling, allowed selection for scorin...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.1080/09553009314552131

    authors： Moore RC,Bender MA

    更新日期：1993-06-01 00:00:00

  • Effects of 50 Hz magnetic fields on cancer induced by ionizing radiation in mice.

    abstract：PURPOSE:To evaluate the effects of 50 Hz magnetic fields (MF) on the development of cancer induced by ionizing radiation. MATERIALS AND METHODS:A total of 150 female CBA/S mice were randomized into three equal groups at the age of 3-5 weeks. One of the groups served as a 'cage-control group'. The two other groups were...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.1080/09553000010024696

    authors： Heikkinen P,Kosma VM,Huuskonen H,Komulainen H,Kumlin T,Penttilä I,Väänänen A,Juutilainen J

    更新日期：2001-04-01 00:00:00

  • Metaphase yields from staphylococcal enterotoxin A stimulated peripheral blood lymphocytes of unirradiated and irradiated aged rhesus monkeys.

    abstract：:The mitogen phytohemagglutinin (PHA) works well in both human and cynomolgus monkey (Macaca fascicularis) lymphocyte cultures to stimulate T cell proliferation. T cells from rhesus monkeys (Macaca mulatta) are less responsive than human cells, producing few metaphases when thousands are required, e.g. in biological do...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.1080/09553009414551321

    authors： Hill FS,Cox AB,Salmon YL,Cantu AO,Lucas JN

    更新日期：1994-10-01 00:00:00

  • Liposome-based delivery of a boron-containing cholesteryl ester for high-LET particle-induced damage of prostate cancer cells: a boron neutron capture therapy study.

    abstract：PURPOSE:The efficacy of a boron-containing cholesteryl ester compound (BCH) as a boron neutron capture therapy (BNCT) agent for the targeted irradiation of PC-3 human prostate cancer cells was examined. MATERIALS AND METHODS:Liposome-based delivery of BCH was quantified with inductively coupled plasma-mass spectrometr...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.3109/09553002.2014.901579

    authors： Gifford I,Vreeland W,Grdanovska S,Burgett E,Kalinich J,Vergara V,Wang CK,Maimon E,Poster D,Al-Sheikhly M

    更新日期：2014-06-01 00:00:00

  • DNA damages processed by base excision repair: biological consequences.

    abstract：:Base damages, sugar damages, and single-strand breaks produced by free radicals are the preponderant lesions produced in DNA by ionizing radiation. These lesions have been individually introduced into substrate, template, and biologically active DNA molecules and enzymatic processing and biological consequences determ...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章,评审

    doi：10.1080/09553009414551661

    authors： Wallace SS

    更新日期：1994-11-01 00:00:00

  • Mechanisms of radiation-induced skin injury and implications for future clinical trials.

    abstract：PURPOSE:To summarize current knowledge regarding mechanisms of radiation-induced skin injury and medical countermeasures available to reduce its severity. Advances in radiation delivery using megavoltage and intensity modulated radiation therapy have permitted delivery of higher doses of radiation to well-defined tumor...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章,评审

    doi：10.3109/09553002.2013.765055

    authors： Kim JH,Kolozsvary AJ,Jenrow KA,Brown SL

    更新日期：2013-05-01 00:00:00

  • X-ray-induced chromosomal aberrations and cell killing in somatic and germ cells of the scid mouse.

    abstract：:To characterize further the radiosensitivity of severe combined immunodeficiency (scid) mice, the induction of micronuclei (MN) in polychromatic erythrocytes as well as cell killing and translocation induction in stem cell spermatogonia was studied. Scid mice turned out to be clearly hypersensitive for X-ray-induced c...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.1080/09553009514550661

    authors： Van Buul PP,De Rooij DG,Zandman IM,Grigorova M,Van Duyn-Goedhart A

    更新日期：1995-05-01 00:00:00

  • Effects of 36.6 GHz and static magnetic field on degree of endoreduplication in Drosophila melanogaster polytene chromosomes.

    abstract：:Purpose To study the effect of microwave (MW) irradiation and consistent action of microwaves and static magnetic field (MF) on the giant chromosomes endoreduplication in Drosophila melanogaster Meig. Materials and methods Experiments were carried out on inbred wild type Canton-S strain. Exposure to microwaves (freque...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.3109/09553002.2016.1137105

    authors： Dyka LD,Shakina LA,Strashnyuk VY,Shckorbatov YG

    更新日期：2016-01-01 00:00:00

  • Environmental and industrial developments in radiation cataractogenesis.

    abstract：:Purpose: This review discusses recent developments in our understanding of biological and physiological mechanisms underlying radiation cataractogenesis. The areas discussed include effects of low-dose exposures to the lens including potential relevance of non-targeted effects, the development of new personal-protecti...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.1080/09553002.2020.1767820

    authors： Laskowski L,Williams D,Seymour C,Mothersill C

    更新日期：2020-05-26 00:00:00

  • Design and characterization of an economical (192)Ir hemi-brain small animal irradiator.

    abstract：PURPOSE:To describe the design and dosimetric characterization of a simple and economical small animal irradiator. MATERIALS AND METHODS:A high dose rate (HDR) (192)Ir brachytherapy source from a commercially available afterloader was used with a 1.3 cm thick tungsten collimator to provide sharp beam penumbra suitable...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.3109/09553002.2014.922719

    authors： Grams MP,Wilson ZC,Sio TT,Beltran CJ,Tryggestad EJ,Gupta SK,Blackwell CR,McCollough KP,Sarkaria JN,Furutani KM

    更新日期：2014-10-01 00:00:00

  • The yield of DNA double-strand breaks produced intracellularly by ionizing radiation: a review.

    abstract：:The mechanisms of radiation damage production are used to examine the following premises: (1) the number of DNA double-strand breaks per unit dose increases with dose; (2) cell type to cell type variations in yield of DNA dsb per dose occur. Two stages of damage production are identified as possible sources of damage ...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章,评审

    doi：10.1080/09553009014551251

    authors： Ward JF

    更新日期：1990-06-01 00:00:00

  • Quantification of DNA damage in patients undergoing non-contrast and contrast enhanced whole body PET/CT investigations using comet assay and micronucleus assay.

    abstract：:Objective: To quantify DNA damage in patients undergoing non-contrast and contrast-enhanced 18F-FDG PET/CT whole body positron emission tomography/computed tomography (WB PET/CT) investigations using comet assay technique and micronucleus assay, and to study the effect of other baseline parameters of patients on DNA d...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.1080/09553002.2019.1577569

    authors： Nautiyal A,Mondal T,Mukherjee A,Mitra D,Kaushik A,Goel HC,Goel A,Dey SK

    更新日期：2019-06-01 00:00:00

  • Radiation-induced bystander phenomenon: insight and implications in radiotherapy.

    abstract：:Despite the extensive utilization of radiotherapy in cancer treatments, clinicians often face challenges in achieving desired outcomes. This is because of the existence of secondary radiation effects like bystander phenomenon that influence radiation nontargeted cells and tissues. The concept of bystander effects of r...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章,评审

    doi：10.1080/09553002.2019.1547440

    authors： Mukherjee S,Chakraborty A

    更新日期：2019-03-01 00:00:00

  • Effects of 50 Hz extremely low frequency sinusoidal magnetic fields on the apoptosis of the hearts of preincubated chicken embryos at different levels of developments.

    abstract：PURPOSE:The aim of this research was to demonstrate the effects of 50 Hz sinusoidal extremely low frequency of magnetic fields (ELF-MF) on preincubated chicken embryo hearts at histopathological, biochemical, and ultrastructural levels. MATERIALS AND METHODS:Ninety healthy fertilized eggs were divided into six groups ...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.3109/09553002.2013.754556

    authors： Shams Lahijani M,Tehrani DM,Fereydouni N

    更新日期：2013-04-01 00:00:00

  • The molecular regulation of apoptosis and implications for radiation oncology.

    abstract：:One of the major goals of cancer research is to identify and understand the causes of cellular proliferation. The role of cell death, or lack thereof, in carcinogenesis, tumour growth, metastatic spread and response to treatment has been largely overlooked even though the morphology of apoptosis (programmed cell death...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章,评审

    doi：10.1080/095530097143789

    authors： Blank KR,Rudoltz MS,Kao GD,Muschel RJ,McKenna WG

    更新日期：1997-05-01 00:00:00

  • Mechanism of protection against radiation-induced DNA damage in plasmid pBR322 by caffeine.

    abstract：PURPOSE:Caffeine (1,3,7-trimethyl xanthine), a dietary component, has been shown to have widely varying effects on DNA damage induced by UV and ionizing radiation, depending upon pre- or post-irradiation administration and its concentration. Caffeine administered post-UV irradiation is known to inhibit enzymatic repair...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.1080/09553000110034649

    authors： Kumar SS,Devasagayam TP,Jayashree B,Kesavan PC

    更新日期：2001-05-01 00:00:00

  • Evidence for a physical component to the radiation-induced bystander effect?

    abstract：PURPOSE:The nature of the transferrable factor which goes from irradiated objects to bystander objects remains undefined. Most agree that a chemical entity is the likely 'factor' although some authors have produced in vitro evidence for the involvement of a physical component or a very potent volatile capable of travel...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.3109/09553002.2012.698366

    authors： Mothersill C,Smith RW,Fazzari J,McNeill F,Prestwich W,Seymour CB

    更新日期：2012-08-01 00:00:00

  • Delayed effects of prenatal low-dose irradiation in the white matter of the rat brain.

    abstract：PURPOSE:This study is part of a general search for the anatomical bases of the severe mental retardation syndrome caused by prenatal irradiation. More specifically, it seeks reasons for the high radiosensitivity of a white matter area, the cingulum of the corpus callosum. MATERIALS AND METHODS:Pregnant primiparous Wis...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.1080/095530099139494

    authors： Reyners H,Gianfelici de Reyners E,Yan J,De Saint-Georges L,Desaintes C

    更新日期：1999-10-01 00:00:00

  • Twenty years of FISH-based translocation analysis for retrospective ionizing radiation biodosimetry.

    abstract：PURPOSE:The fluorescent in situ hybridization (FISH) technique, which easily detects reciprocal translocations, is currently used to estimate doses in retrospective biological dosimetry, after suspected accidental overexposure to ionizing radiation (IR). This study of 42 cases aimed to verify the appropriateness of thi...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.1080/09553002.2018.1427903

    authors： Grégoire E,Roy L,Buard V,Delbos M,Durand V,Martin-Bodiot C,Voisin P,Sorokine-Durm I,Vaurijoux A,Voisin P,Baldeyron C,Barquinero JF

    更新日期：2018-03-01 00:00:00

  • Radiation-induced gene expression in MCF-7 cells.

    abstract：PURPOSE:Detection of gene targets applicable to biological dosimetry and early detection of radiation-induced cell damage. MATERIALS AND METHODS:MCF-7 human mammary carcinoma cells were exposed to 2 and 6 Gy X-rays. Expression of 1176 genes was traced with the Atlas Human 1.2 Array (Clontech). RESULTS:(1) Based on da...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.1080/0955300032000093146

    authors： Stassen T,Port M,Nuyken I,Abend M

    更新日期：2003-05-01 00:00:00

  • Repetitive 50 Hz pulsed electromagnetic field ameliorates the diabetes-induced impairments in the relaxation response of rat thoracic aorta rings.

    abstract：PURPOSE:To evaluate the characteristic features of mechanical responses and the membrane potential changes induced by repetitive pulsed electromagnetic field (PEMF, 50 Hz, 5 mT) in thoracic aorta rings obtained from streptozotocin-induced diabetic and healthy control rats to determine if PEMF could ameliorate problems ...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.1080/09553000903009522

    authors： Kavak S,Emre M,Meral I,Unlugenc H,Pelit A,Demirkazik A

    更新日期：2009-08-01 00:00:00

  • Physical and biophysical properties of proton tracks of energies 1 keV to 300 MeV in water.

    abstract：PURPOSE:To investigate physical and biophysical properties of proton tracks 1 keV-300 MeV using Monte Carlo track structure methods. MATERIALS AND METHODS:We present model calculations for cross sections and methods for simulations of full-slowing-down proton tracks. Protons and electrons were followed interaction-by-...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.3109/09553002.2010.518204

    authors： Liamsuwan T,Uehara S,Emfietzoglou D,Nikjoo H

    更新日期：2011-02-01 00:00:00

  • Heat effects on the repair of DNA double-strand breaks in CHO cells.

    abstract：:The effect of heat (43-45 degrees C) on the induction and the repair of DNA double-strand breaks (dsb) was studied in CHO cells after 60 Gy of X-rays using constant-field gel electrophoresis. Heat given prior to irradiation was found not to alter the number of dsb when measured immediately after irradiation. In non-he...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.1080/095530097143392

    authors： Dahm-Daphi J,Brammer I,Dikomey E

    更新日期：1997-08-01 00:00:00

  • 50-Hz magnetic field induces EGF-receptor clustering and activates RAS.

    abstract：PURPOSE:In a previous study, we found that exposure to a 50 Hz magnetic field (MF) could activate stress-activated protein kinase (SAPK) and P38 mitogen-activated protein (MAP) kinase (P38 MAPK) in Chinese hamster lung (CHL) fibroblast cells, and simultaneous exposure to a 'noise' MF of the same intensity inhibited the...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.1080/09553000801998875

    authors： Ke XQ,Sun WJ,Lu DQ,Fu YT,Chiang H

    更新日期：2008-05-01 00:00:00

  • Role of a high throughput biodosimetry test in treatment prioritization after a nuclear incident.

    abstract：PURPOSE:We introduce and evaluate a high throughput biodosimetry test system (REDI-Dx) suitable for testing of thousands of potential radiation victims following a mass scale nuclear event caused by detonation of a nuclear device or a nuclear accident, as part of an overall strategy for effective medical management of ...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.1080/09553002.2018.1532615

    authors： Jacobs AR,Guyon T,Headley V,Nair M,Ricketts W,Gray G,Wong JYC,Chao N,Terbrueggen R

    更新日期：2020-01-01 00:00:00

  • Adoptive transfer of cytotoxic T-cells for treatment of residual disease after irradiation.

    abstract：PURPOSE:To evaluate whether immunotherapy based on adoptively transferred cytotoxic T-cells (CTL) can improve the antitumour efficacy of irradiation. MATERIAL AND METHODS:The experiments were performed using the human squamous cell carcinoma line UT-SCC-15, which expresses human leukocyte antigen (HLA)-A2. The UT-SCC-...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.1080/09553000701570196

    authors： Krause M,Schmitz M,Noessner E,Skrablin PS,Wehner R,Rieber EP,Baumann M

    更新日期：2007-11-01 00:00:00

  • Proliferation saturation index in an adaptive Bayesian approach to predict patient-specific radiotherapy responses.

    abstract：:Purpose: Radiotherapy prescription dose and dose fractionation protocols vary little between individual patients having the same tumor grade and stage. To personalize radiotherapy a predictive model is needed to simulate radiation response. Previous modeling attempts with multiple variables and parameters have been sh...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.1080/09553002.2019.1589013

    authors： Sunassee ED,Tan D,Ji N,Brady R,Moros EG,Caudell JJ,Yartsev S,Enderling H

    更新日期：2019-10-01 00:00:00

  • Nuclear structure and the microdistribution of radiation damage in DNA.

    abstract：:Evidence for the roles of proteins and metal ions in the microheterogeneity of DNA damage is reviewed. Decondensation of chromatin in hypotonic buffers markedly sensitizes the DNA to radiation, while treatment of nuclei with hypertonic buffers strips the DNA of histones and other nuclear proteins and enhances the radi...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章,评审

    doi：10.1080/09553009414551561

    authors： Oleinick NL,Balasubramaniam U,Xue L,Chiu S

    更新日期：1994-11-01 00:00:00

  • The effect of the radioprotector WR-2721 and WR-1065 on mitochondrial lipid peroxidation.

    abstract：:The radioprotective agent WR-2721 is dephosphorylated to the free thiol form WR-1065 in vivo. The effects of WR-2721, WR-1065 and reduced glutathione on a mitochondrial lipid peroxidation system were compared. WR-2721 had no effect on mitochondrial lipid peroxidation in vitro, and could not prevent the inactivation of...

    journal_title：International journal of radiation biology

    pub_type： 杂志文章

    doi：10.1080/09553009014552611

    authors： Tretter L,Rónai E,Szabados G,Hermann R,Andó A,Horváth I

    更新日期：1990-03-01 00:00:00