T2 relaxation reveals spatial collagen architecture in articular cartilage: a comparative quantitative MRI and polarized light microscopic study.

Abstract:

:It has been suggested that orientational changes in the collagen network of articular cartilage account for the depthwise T2 anisotropy of MRI through the magic angle effect. To investigate the relationship between laminar T2 appearance and collagen organization (anisotropy), bovine osteochondral plugs (N = 9) were T2 mapped at 9.4T with cartilage surface normal to the static magnetic field. Collagen fibril arrangement of the same samples was studied with polarized light microscopy, a quantitative technique for probing collagen organization by analyzing its ability to rotate plane polarized light, i.e., birefringence (BF). Depthwise variation of safranin O-stained proteoglycans was monitored with digital densitometry. The spatially varying cartilage T2 followed the architectural arrangement of the collagen fibril network: a linear positive correlation between T2 and the reciprocal of BF was established in each sample, with r = 0.91 +/- 0.02 (mean +/- SEM, N = 9). The current results reveal the close connection between the laminar T2 structure and the collagen architecture in histologic zones.

journal_name

Magn Reson Med

authors

Nieminen MT,Rieppo J,Töyräs J,Hakumäki JM,Silvennoinen J,Hyttinen MM,Helminen HJ,Jurvelin JS

doi

10.1002/mrm.1218

subject

Has Abstract

pub_date

2001-09-01 00:00:00

pages

487-93

issue

3

eissn

0740-3194

issn

1522-2594

pii

10.1002/mrm.1218

journal_volume

46

pub_type

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