Cloning, functional expression and tissue distribution of the human P2Y6 receptor.

abstract：:Characterization of functional vessels is required either for monitoring hemodynamics or patterning of functional vasculature in experimental models. Haemoglobin (Hb) staining is a traditionally used approach for determining the differentiation of erythroid cells. In this investigation, we tested if HB staining can be...

journal_title：Biochemical and biophysical research communications

authors： Zheng PP,Severijnen LA,Willemsen R,Kros JM

更新日期：2009-03-20 00:00:00

abstract：:An increase in the intracellular cAMP concentration induces tyrosine phosphorylation of the epidermal growth factor receptor (EGFR) followed by activation of extracellular signal-regulated kinases 1/2 (ERK1/2). In this report we demonstrate that these effects of cAMP are mediated via activation of protein kinase A (PK...

journal_title：Biochemical and biophysical research communications

authors： Piiper A,Lutz MP,Cramer H,Elez R,Kronenberger B,Dikic I,Müller-Esterl W,Zeuzem S

更新日期：2003-02-21 00:00:00

abstract：:To investigate the involvement of thyroid hormone on the release of atrial natriuretic polypeptide (ANP), we have measured immunoreactive ANP in the atria and plasma of experimental hyperthyroid and hypothyroid rats. Plasma ANP was higher (p less than 0.05) in hyperthyroid rats and was lower (p less than 0.05) in hypo...

journal_title：Biochemical and biophysical research communications

authors： Kohno M,Takaori K,Matsuura T,Murakawa K,Kanayama Y,Takeda T

更新日期：1986-01-14 00:00:00

abstract：:A prolyl isomerase Pin1 deficient (Pin1-/-) male mice had severe testicular atrophy. We investigated the function of Pin1 in spermatogenesis by analyzing the Pin1-/- mice at reproductive age. Pin1-/- mice had lessαPLZF positive spermatogonia (undifferentiated spermatogonia) than wild type (WT). Nevertheless, the Pin1-...

journal_title：Biochemical and biophysical research communications

authors： Kurita-Suzuki A,Kamo Y,Uchida C,Tanemura K,Hara K,Uchida T

更新日期：2018-02-26 00:00:00

abstract：:The human pancreas carcinoma cell line T3M-4 contains activated c-Kirsten (K)-ras oncogene detectable by the DNA-mediated gene transfer technique using NIH/3T3 cells. DNA fragments containing coding lesions have been cloned, and nucleotide sequence analysis suggests that the T3M-4 oncogene has been activated by a sing...

journal_title：Biochemical and biophysical research communications

authors： Hirai H,Okabe T,Anraku Y,Fujisawa M,Urabe A,Takaku F

更新日期：1985-02-28 00:00:00

abstract：:Actin is a highly abundant cytoskeletal protein that is essential for all eukaryotic cells and participates in many structural and functional roles. It has long been noted that estrogen affects cellular morphology. However, recent studies observed that both estrogen and tamoxifen induce a remarkable cytoskeletal remod...

journal_title：Biochemical and biophysical research communications

authors： Wang Z,Sun L,Liang S,Liu ZC,Zhao ZY,Yang J,Wang D,Yang DQ

更新日期：2019-08-27 00:00:00

abstract：:This article has been withdrawn at the request of the editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...

journal_title：Biochemical and biophysical research communications

authors： Rai V,Datta K

更新日期：2010-04-10 00:00:00

abstract：:Apobec1 edits the ApoB mRNA by deaminating nucleotide C(6666), which results in a codon change from Glutamate to stop, and subsequent expression of a truncated protein. Apobec1 is regulated by ACF (Apobec1 complementation factor) and hnRNPQ, which contains an N-terminal "acidic domain" (AcD) of unknown function, three...

journal_title：Biochemical and biophysical research communications

authors： Quaresma AJ,Oyama S Jr,Barbosa JA,Kobarg J

更新日期：2006-11-17 00:00:00

abstract：:Neurodegeneration causes dysfunction and degeneration of neurons and is triggered by various factors including genetic defects, free radicals, injury, and glutamate excitotoxicity. Among those, glutamate excitotoxicity is implicated in chronic disorders including AD and ALS, and in acute insults in the CNS including t...

journal_title：Biochemical and biophysical research communications

authors： Fujiwara T,Morimoto K

更新日期：2013-10-25 00:00:00

abstract：:Thermo-alkalophilic bacterium, Geobacillus thermoleovorans secrets many enzymes including a 43 kDa extracellular lipase. Significant thermostability, organic solvent stability and wide substrate preferences for hydrolysis drew our attention to solve its structure by crystallography. The structure was solved by molecul...

journal_title：Biochemical and biophysical research communications

authors： Moharana TR,Pal B,Rao NM

更新日期：2019-01-01 00:00:00

abstract：:miR-4458, a new tumor-suppressor, was reported to down-regulated in human hepatocellular carcinoma. The expression status, roles and inhibitory mechanisms of miR-4458 in other tumors still need to be clarified. The aim of this study is to investigate the effects of miR-4458 and to elucidate the potential mechanism in ...

journal_title：Biochemical and biophysical research communications

authors： Qin Y,Cheng C,Lu H,Wang Y

更新日期：2016-01-01 00:00:00

abstract：:Cytosolic free calcium concentration ([Ca(2+)]i) is a central signalling element for the maintenance of endothelial barrier function. Under physiological conditions, it is controlled within narrow limits. Metabolic inhibition during ischemia/reperfusion, however, induces [Ca(2+)]i overload, which results in barrier fa...

journal_title：Biochemical and biophysical research communications

authors： Bosche B,Schäfer M,Graf R,Härtel FV,Schäfer U,Noll T

更新日期：2013-05-03 00:00:00

abstract：:This is the first study to demonstrate that the AT motif binding factor 1-A (ATBF1-A) is expressed in the crypts and the bases of villi of the small intestine and negatively regulates transcription of brush-border enzyme gene, aminopeptidase-N (APN). In situ hybridization visualized a limited ATBF1-A mRNA expression i...

journal_title：Biochemical and biophysical research communications

authors： Kataoka H,Joh T,Miura Y,Tamaoki T,Senoo K,Ohara H,Nomura T,Tada T,Asai K,Kato T,Itoh M

更新日期：2000-01-07 00:00:00

abstract：:Tamoxifen (TAM), an anti-estrogen compound, is widely used for chemotherapy of breast cancer, although the molecular mechanisms underlying TAM cytotoxicity are obscure. Here, we show that TAM dramatically caused degradation of vimentin (VIM) in human skin fibroblasts, in a time and dose dependent manner. Addition of c...

journal_title：Biochemical and biophysical research communications

authors： Hashimoto M,Inoue S,Ogawa S,Conrad C,Muramatsu M,Shackelford D,Masliah E

更新日期：1998-06-18 00:00:00

abstract：:Wet elastoidin spicules (fish fin rays) yield low-angle meridional X-ray diffraction patterns which resemble those from tendons. However, when the spicule dries the meridian splits into the arms of a diagonal cross (sometimes only one arm appears). Of the possible explanations we reject shearing of the axial arrangeme...

journal_title：Biochemical and biophysical research communications

authors： Hukins DW,Woodhead-Galloway J,Knight DP

更新日期：1976-12-20 00:00:00

abstract：:Cell-free translation is one potential approach to the production of functional transmembrane proteins. We have now examined various detergents as supplements to a wheat-germ cell-free system in order to optimize the production and subsequent purification of a functional model transmembrane protein, bacteriorhodopsin....

journal_title：Biochemical and biophysical research communications

authors： Genji T,Nozawa A,Tozawa Y

更新日期：2010-10-01 00:00:00

abstract：:The genome of a novel foot-and-mouth disease virus, HKN/2002, was 8104 nucleotides (nt) in length (excluding the poly(C) tract and poly(A) tail) and was composed of a 1042-nt 5'-untranslated region (UTR), a 6966-nt open reading frame, and a 93-nt 3'-UTR. Genome sequences of HKN/2002 and other known FMDV strains were c...

journal_title：Biochemical and biophysical research communications

authors： Feng Q,Yu H,Liu Y,He C,Hu J,Sang H,Ding N,Ding M,Fung YW,Lau LT,Yu AC,Chen J

更新日期：2004-10-08 00:00:00

abstract：:Lipid rafts often serve as an entry site for certain viruses. Here, we report that lipid rafts in Vero E6 cells are involved in the entry of severe acute respiratory syndrome coronavirus (SARS-CoV). Infectivity assay showed the integrity of lipid rafts was required for productive infection of pseudotyped SARS-CoV. Dep...

journal_title：Biochemical and biophysical research communications

authors： Lu Y,Liu DX,Tam JP

更新日期：2008-05-02 00:00:00

abstract：:Mutations in the WNK1 and WNK4 genes result in an inherited hypertensive disease, pseudohypoaldosteronism type II (PHAII). Recently, the KLHL3 and Cullin3 genes were also identified as responsible genes for PHAII. Although we have reported that WNK4 is a substrate for the KLHL3-Cullin3 E3 ligase complex, it is not cle...

journal_title：Biochemical and biophysical research communications

authors： Takahashi D,Mori T,Wakabayashi M,Mori Y,Susa K,Zeniya M,Sohara E,Rai T,Sasaki S,Uchida S

更新日期：2013-08-02 00:00:00

abstract：:Aberrant expression of cell cycle regulators have been implicated in prostate cancer development and progression. Therefore, understanding transcriptional networks controlling the cell cycle remain a challenge in the development of prostate cancer treatment. In this study, we found that icilin, a super-cooling agent, ...

journal_title：Biochemical and biophysical research communications

authors： Lee S,Chun JN,Kim SH,So I,Jeon JH

更新日期：2013-11-29 00:00:00

abstract：:We have purified and isolated a novel, hypotensive peptide from avian ventricular tissue. Ventricular homogenates have been shown to exhibit potent hypotensive activity in the avian and mammalian species, with little natriuresis or diuresis. Using avian mean arterial pressure (MAP) as a bioassay, we were able to purif...

journal_title：Biochemical and biophysical research communications

authors： Barbato GF,Wideman RF

更新日期：1990-06-29 00:00:00

abstract：:We find that the low frequency Raman spectrum of Zn(II) metallothionein has a single prominent band at 138 cm-1 which is absent from the Raman spectrum of the metal-free protein. This feature is also found for Cd(II) binding to both of the independent metallothionein domains and the metallothionein from Neurospora cra...

journal_title：Biochemical and biophysical research communications

authors： Elgren TE,Wilcox DE

更新日期：1989-09-15 00:00:00

abstract：:The aim of this study was to investigate the possible pathological relation between mechanical stress and hyperpigmentation. We did this by investigating the influence of cyclic stretch on the expression of keratinocyte- and fibroblast-derived melanogenetic paracrine cytokines in vitro. Using primary human keratinocyt...

journal_title：Biochemical and biophysical research communications

authors： Kurita M,Okazaki M,Fujino T,Takushima A,Harii K

更新日期：2011-05-27 00:00:00

abstract：:The 5'-flanking region of the human poly(ADP-ribose) polymerase gene was isolated and characterized. The nucleotide sequence of a part of the poly(ADP-ribose) polymerase gene completely matched that of the cDNA. The transcriptional initiation sites (cap sites) of this gene, located about 166-bp upstream from the trans...

journal_title：Biochemical and biophysical research communications

authors： Ogura T,Nyunoya H,Takahashi-Masutani M,Miwa M,Sugimura T,Esumi H

更新日期：1990-03-16 00:00:00

abstract：:Due to their spontaneous accumulation in inflamed or infected areas, blood phagocytes are potent drug vectors with specific targeting. Drug like molecule loading was obtained by use of cell electropermeabilization in which the impermeability of their plasma membrane is transiently impaired. Electrical conditions were ...

journal_title：Biochemical and biophysical research communications

authors： Sixou S,Teissié J

更新日期：1992-07-31 00:00:00

abstract：:The serotonin(1A) (5-HT(1A)) receptor is an important member of the superfamily of seven transmembrane domain G-protein-coupled receptors. We report here that solubilization of the hippocampal 5-HT(1A) receptor by the zwitterionic detergent CHAPS is accompanied by loss of membrane cholesterol which results in a reduct...

journal_title：Biochemical and biophysical research communications

authors： Chattopadhyay A,Jafurulla M,Kalipatnapu S,Pucadyil TJ,Harikumar KG

更新日期：2005-02-25 00:00:00

abstract：:Human serum albumin (HSA) serves as a natural depot of amyloid β peptide (Aβ). Improvement of Aβ binding to HSA should impede Alzheimer's disease (AD). We developed a method for quantitation of the interaction between monomeric Aβ40/42 and HSA using surface plasmon resonance spectroscopy. The dissociation constant of ...

journal_title：Biochemical and biophysical research communications

authors： Litus EA,Kazakov AS,Sokolov AS,Nemashkalova EL,Galushko EI,Dzhus UF,Marchenkov VV,Galzitskaya OV,Permyakov EA,Permyakov SE

更新日期：2019-03-05 00:00:00

abstract：:Runt-related transcription factor-2 (RUNX2)/core binding factor a1 (Cbfa1) is implicated in the regulation of osteoblast differentiation and osteoblast-specific gene expression. Mutations in RUNX2 cause the bone disease cleidocranial dysplasia, which is characterized by multiple skeletal defects. RUNX2 is expressed as...

journal_title：Biochemical and biophysical research communications

authors： Sudhakar S,Li Y,Katz MS,Elango N

更新日期：2001-11-30 00:00:00

abstract：:Ribosome modulation factor (RMF) is involved in stabilization of ribosomes during the transition from exponential growth to the stationary growth phase in Escherichia coli. A deficiency of RMF is known to reduce cell viability. Overaccumulation of spermidine also leads to a decrease in cell viability and to a decrease...

journal_title：Biochemical and biophysical research communications

authors： Apirakaramwong A,Fukuchi J,Kashiwagi K,Kakinuma Y,Ito E,Ishihama A,Igarashi K

更新日期：1998-10-20 00:00:00

abstract：:We have identified a novel reaction in which acetaldehyde promotes rat hepatic cytosolic catalysis of O2 consumption coupled with glutathione oxidation without apparent release of activated forms of O2. Acetaldehyde is not consumed in the reaction. The reaction (O2 consumption or oxidized glutathione production) is sa...

journal_title：Biochemical and biophysical research communications

authors： Lyon I,Kaplowitz N

更新日期：1985-06-28 00:00:00