当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > In vivo targeting of inflamed areas by electroloaded neutrophils.

In vivo targeting of inflamed areas by electroloaded neutrophils.

Abstract：

:Due to their spontaneous accumulation in inflamed or infected areas, blood phagocytes are potent drug vectors with specific targeting. Drug like molecule loading was obtained by use of cell electropermeabilization in which the impermeability of their plasma membrane is transiently impaired. Electrical conditions were used which allow electroloading of a drug like molecule (propidium iodide) in 70% of leukocytes in a whole blood sample while preserving in vitro functional properties. Slow release of entrapped hydrophilic molecules was observed with a half lifetime longer than 4 hours at 4 degrees C and at 37 degrees C. With an in vivo assay, using a rat model of inflammation, we showed that, as for non-pulsed cells, pulsed neutrophils accumulate 10 times more in an inflamed area than they do in control areas. Phagocyte electropermeabilization is therefore a very efficient way of drug targeting. Accumulation of electropulsed neutrophils in an area of inflammation gives targeted release of the electroloaded drug.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Sixou S,Teissié J

doi

10.1016/0006-291x(92)90825-6

subject

Has Abstract

pub_date

1992-07-31 00:00:00

pages

860-6

issue

2

eissn

0006-291X

issn

1090-2104

pii

0006-291X(92)90825-6

journal_volume

186

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Interaction of Trypanosoma cruzi with cells with altered glycosylation patterns.

    abstract：:Glycosylation mutants of chinese hamster ovary cells were used to analyse the role played by surface-exposed carbohydrates on the process of interaction of Trypanosoma cruzi with the host cell. Adhesion and invasion of the parasites were markedly reduced in cells which express very few sialic acid residues. Infection ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1684

    authors： Ciavaglia Mdo C,de Carvalho TU,de Souza W

    更新日期：1993-06-15 00:00:00

  • Effects of hydrogen sulfide on homocysteine-induced oxidative stress in vascular smooth muscle cells.

    abstract：:Hydrogen sulfide (H(2)S) is an important gasotransmitter that generated in mammalian cells from l-cysteine metabolism. Little is known about its protective role in oxidative stress. In the present study, we investigated whether H(2)S could affect homocysteine (HCY)-induced cytotoxicity and oxidative stress in vascular...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.10.058

    authors： Yan SK,Chang T,Wang H,Wu L,Wang R,Meng QH

    更新日期：2006-12-15 00:00:00

  • Structural organization of the human selenium-dependent phospholipid hydroperoxide glutathione peroxidase gene (GPX4): chromosomal localization to 19p13.3.

    abstract：:The primary structure of human selenium-dependent phospholipid hydroperoxide glutathione peroxidase (GPX4) was determined by genomic cloning. The gene structure of GPX4 spans only 2.8 kb and consists of 7 exons. The coding sequence resides on all 7 exons, and the mitochondrial leader sequence is contained entirely wit...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9086

    authors： Kelner MJ,Montoya MA

    更新日期：1998-08-10 00:00:00

  • Impact of methionine oxidation on calmodulin structural dynamics.

    abstract：:We have used electron paramagnetic resonance (EPR) to examine the structural impact of oxidizing specific methionine (M) side chains in calmodulin (CaM). It has been shown that oxidation of either M109 or M124 in CaM diminishes CaM regulation of the muscle calcium release channel, the ryanodine receptor (RyR), and tha...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.11.091

    authors： McCarthy MR,Thompson AR,Nitu F,Moen RJ,Olenek MJ,Klein JC,Thomas DD

    更新日期：2015-01-09 00:00:00

  • Conversion of nicotinic acid to trigonelline is catalyzed by N-methyltransferase belonged to motif B' methyltransferase family in Coffea arabica.

    abstract：:Trigonelline (N-methylnicotinate), a member of the pyridine alkaloids, accumulates in coffee beans along with caffeine. The biosynthetic pathway of trigonelline is not fully elucidated. While it is quite likely that the production of trigonelline from nicotinate is catalyzed by N-methyltransferase, as is caffeine synt...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.09.043

    authors： Mizuno K,Matsuzaki M,Kanazawa S,Tokiwano T,Yoshizawa Y,Kato M

    更新日期：2014-10-03 00:00:00

  • Role of protein kinase C-alpha in activation of ecto-5'-nucleotidase in the preconditioned canine myocardium.

    abstract：:We have reported that activation of protein kinase C (PKC) increases ecto-5'-nucleotidase activity, which may contribute to the infarct size-limiting effect of ischemic preconditioning. Since we have reported that Ca(2+)- and phospholipid-sensitive PKC is activated due to ischemic preconditioning, we further tested 1)...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7445

    authors： Kitakaze M,Funaya H,Minamino T,Node K,Sato H,Ueda Y,Okuyama Y,Kuzuya T,Hori M,Yoshida K

    更新日期：1997-10-09 00:00:00

  • Molecular cloning of cDNA for rat glycine methyltransferase.

    abstract：:Using a highly purified preparation of glycine methyltransferase mRNA, double-stranded cDNA was synthesized and inserted into the PstI site of pBR322. The resulting recombinant DNA was used to transform E. coli X 1776 by conventional methods. Among tetracycline-resistant transformants, a number of colonies were found ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)90913-6

    authors： Ogawa H,Gomi T,Horii T,Ogawa H,Fujioka M

    更新日期：1984-10-15 00:00:00

  • Insulin/NFκB protects against ischemia-induced necrotic cardiomyocyte death.

    abstract：:In the heart, insulin controls key functions such as metabolism, muscle contraction and cell death. However, all studies have been focused on insulin action during reperfusion. Here we explore the cardioprotective action of this hormone during ischemia. Rat hearts were perfused ex vivo with an ischemia/reperfusion Lan...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.09.171

    authors： Díaz A,Humeres C,González V,Gómez MT,Montt N,Sanchez G,Chiong M,García L

    更新日期：2015-11-13 00:00:00

  • Activation mechanism of solubilized epidermal growth factor receptor tyrosine kinase.

    abstract：:Dimerization of epidermal growth factor receptor (EGFR) leads to the activation of its tyrosine kinase. To elucidate whether dimerization is responsible for activation of the intracellular tyrosine kinase domain or just plays a role in the stabilization of the active form, the activated status of wild-type EGFR moiety...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.6285

    authors： Ge G,Wu J,Wang Y,Lin Q

    更新日期：2002-01-25 00:00:00

  • Conversion of desulforedoxin into a rubredoxin center.

    abstract：:Rubredoxin and desulforedoxin both contain an Fe(S-Cys)4 center. However, the spectroscopic properties of the center in desulforedoxin differ from rubredoxin. These differences arise from a distortion of the metal site hypothesized to result from adjacent cysteine residues in the primary sequence of desulforedoxin. Tw...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6171

    authors： Yu L,Kennedy M,Czaja C,Tavares P,Moura JJ,Moura I,Rusnak F

    更新日期：1997-02-24 00:00:00

  • Identification and characterization of proteins involved in stolon adhesion in the highly invasive fouling ascidian Ciona robusta.

    abstract：:Adhesive ascidians have caused serious biofouling problems and huge economic losses in marine ecosystems. However, adhesion mechanisms, particularly on functional proteins involved in ascidian adhesion, remain largely unexplored. Here, we identified 26 representative stolon proteins from the highly invasive fouling as...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.01.053

    authors： Li S,Huang X,Chen Y,Li X,Zhan A

    更新日期：2019-02-26 00:00:00

  • Age-dependent and cell-population-restricted LRRK2 expression in normal mouse spleen.

    abstract：:Leucine-rich repeat kinase 2 (LRRK2) is the causal molecule of familial Parkinson's disease (PD), but its true physiological function remains unknown. In the normal mouse, LRRK2 is expressed in kidney, spleen, and lung at much higher levels than in brain, suggesting that LRRK2 may play an important role in these organ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.01.041

    authors： Maekawa T,Kubo M,Yokoyama I,Ohta E,Obata F

    更新日期：2010-02-12 00:00:00

  • Hydrolysis of oxidized nucleotides by the Escherichia coli Orf135 protein.

    abstract：:To examine the possibility that the Orf135 protein of Escherichia coli functions as a hydrolyzing enzyme for a damaged DNA precursor (deoxyribonucleoside 5'-triphosphate), we purified the recombinant Orf135 protein and incubated it with oxidized deoxynucleotides. Of the nucleotides tested, 2-hydroxydeoxyadenosine 5'-t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5781

    authors： Kamiya H,Murata-Kamiya N,Iida E,Harashima H

    更新日期：2001-11-02 00:00:00

  • A cryptic protease activity from Trypanosoma cruzi revealed by preincubation with kininogen at low temperatures.

    abstract：:Cysteine proteases have been identified in parasitic protozoa including the causative agent of Chagas' disease Trypanosoma cruzi. T. cruzi lysates subjected to substrate-containing SDS-polyacrylamide gel electrophoresis exhibit major bands of proteolytic activity in the 45-55 kDa molecular mass range (cruzipain activi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7690

    authors： Wiser MF,Lonsdale-Eccles JD,D'Alessandro A,Grab DJ

    更新日期：1997-11-26 00:00:00

  • Identification of Gliotoxin isolated from marine fungus as a new pyruvate kinase M2 inhibitor.

    abstract：:Pyruvate kinase M2 (PKM2) functions as an important rate-limiting enzyme of aerobic glycolysis that is involved in tumor initiation and progression. However, there are few studies on effective PKM2 inhibitors. Gliotoxin is a marine-derived fungal secondary metabolite with multiple biological activities, including immu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.05.139

    authors： Tang W,Liu ZL,Mai XY,Qi X,Li DH,Gu QQ,Li J

    更新日期：2020-07-30 00:00:00

  • QCM-4, a 5-HT₃ receptor antagonist ameliorates plasma HPA axis hyperactivity, leptin resistance and brain oxidative stress in depression and anxiety-like behavior in obese mice.

    abstract：:Several preclinical studies have revealed antidepressant and anxiolytic-like effect of 5-HT3 receptor antagonists. In our earlier study, we have reported the antidepressive-like effect of 3-methoxy-N-p-tolylquinoxalin-2-carboxamide (QCM-4) in obese mice subjected to chronic stress. The present study deals with the bio...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.11.036

    authors： Kurhe Y,Mahesh R,Devadoss T

    更新日期：2015-01-02 00:00:00

  • Transcriptional regulation of the human Liver X Receptor α gene by Hepatocyte Nuclear Factor 4α.

    abstract：:Liver X Receptors (LXRs) are sterol-activated transcription factors that play major roles in cellular cholesterol homeostasis, HDL biogenesis and reverse cholesterol transport. The aim of the present study was to investigate the mechanisms that control the expression of the human LXRα gene in hepatic cells. A series o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.12.031

    authors： Theofilatos D,Anestis A,Hashimoto K,Kardassis D

    更新日期：2016-01-15 00:00:00

  • Oxidation of lysine side-chains of elastin by the myeloperoxidase system and by stimulated human neutrophils.

    abstract：:Exposure of [3H]-lysine labeled elastin to either purified myeloperoxidase plus H2O2 and halides or human neutrophils plus phorbol myristate acetate resulted in oxidation of lysine side chains quantitated as 3H2O release. In both the enzyme and cell system oxidation was blocked by azide, cyanide or catalase, but not b...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90015-x

    authors： Clark RA,Szot S,Williams MA,Kagan HM

    更新日期：1986-03-13 00:00:00

  • Long-lived species have improved proteostasis compared to phylogenetically-related shorter-lived species.

    abstract：:Our previous studies have shown that the liver from Naked Mole Rats (NMRs), a long-lived rodent, has increased proteasome activity and lower levels of protein ubiquitination compared to mice. This suggests that protein quality control might play a role in assuring species longevity. To determine whether enhanced prote...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.01.046

    authors： Pride H,Yu Z,Sunchu B,Mochnick J,Coles A,Zhang Y,Buffenstein R,Hornsby PJ,Austad SN,Pérez VI

    更新日期：2015-02-20 00:00:00

  • S100 to receptor for advanced glycation end-products binding assay: looking for inhibitors.

    abstract：:Secreted by tumor and stromal cells, S100 proteins exert their biological functions via the interaction with surface receptors. The most described receptor is the receptor for advanced glycation end-products (RAGE), thereby participating in the S100-dependent cell migration, invasion, tumor growth, angiogenesis and me...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.02.143

    authors： Padilla L,Dakhel S,Hernández JL

    更新日期：2014-03-28 00:00:00

  • Influence of cyclic nucleotides on the processing of the carbohydrate part of the alpha-subunit of human choriogonadotropin by first trimester human placenta tissue.

    abstract：:In pulse-chase experiments ([35S]Met as radioactive label) 4 intracellular forms of the alpha-subunit (apparent molecular weights of 11, 16.5, 19.5, and 23.4 kDa) were observed whereas almost no label was incorporated into the beta-subunit. The 23.4 kDa form was secreted as free alpha-subunit, the others were precurso...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92399-1

    authors： Hilf G,Merz WE

    更新日期：1989-02-28 00:00:00

  • Porcine guanylin and uroguanylin: cDNA sequences, deduced amino acid sequences, and biological activity of the chemically synthesized peptides.

    abstract：:Guanylin and uroguanylin are structurally related intestinal peptide hormones which were purified from a limited number of mammals and are capable of activating the particulate guanylate cyclase-C. Although the biological functions of guanylin and uroguanylin are not yet clarified in detail, they are involved in the r...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0719

    authors： Mägert HJ,Hill O,Zucht HD,Martin S,Meyer M,Forssmann WG,Adermann K

    更新日期：1999-05-27 00:00:00

  • Phytol increases adipocyte number and glucose tolerance through activation of PI3K/Akt signaling pathway in mice fed high-fat and high-fructose diet.

    abstract：:It has been shown that adipose tissue hyperplasia (increased adipocyte number or adipogenesis) has beneficial effects on metabolic health. The aim of the present study was to determine whether phytol could modulate hyperplasia/adipogenesis and glucose homeostasis, and to explore the underlying mechanisms in mice fed h...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.05.160

    authors： Wang J,Hu X,Ai W,Zhang F,Yang K,Wang L,Zhu X,Gao P,Shu G,Jiang Q,Wang S

    更新日期：2017-08-05 00:00:00

  • Differential sensitivity of arachidonic acid release and 1,2-diacylglycerol formation to pertussis toxin, GDP beta S and NaF in saponin-permeabilized human platelets: possible evidence for distinct GTP-binding proteins involving phospholipase C and A2 act

    abstract：:Human platelets labeled with [3H]arachidonic acid and permeabilized with saponin produced [3H]1,2-diacylglycerol (DG) by phospholipase C and released [3H]arachidonate by phospholipase A2, when activated with thrombin. Thrombin-induced arachidonate liberation was almost completely inhibited with pretreatment of pertuss...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(87)80227-9

    authors： Nakashima S,Hattori H,Shirato L,Takenaka A,Nozawa Y

    更新日期：1987-11-13 00:00:00

  • Structural modeling of SoxF protein from Chlorobium tepidum: an approach to understand the molecular basis of thiosulfate oxidation.

    abstract：:Microbial redox reactions of inorganic sulfur compounds play a vital role in balancing the turnover of this element in the environment. These vital reactions are carried out by the enzyme system encoded by the sox operon. The central player of the sulfur oxidation biochemistry is the SoxY-Z protein complex. Another pr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.09.101

    authors： Bagchi A

    更新日期：2011-10-22 00:00:00

  • A novel in vivo regulatory role of P-glycoprotein in alloimmunity.

    abstract：:P-glycoprotein (P-gp) is required for adaptive immunity through defined functions in T cell activation and antigen presenting cell (APC) maturation. The potential role of P-gp as an in vivo regulator of alloimmunity is currently unknown. Here we show that P-gp blockade prolongs graft survival in a murine heterotopic c...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.03.040

    authors： Izawa A,Schatton T,Frank NY,Ueno T,Yamaura K,Pendse SS,Margaryan A,Grimm M,Gasser M,Waaga-Gasser AM,Sayegh MH,Frank MH

    更新日期：2010-04-09 00:00:00

  • Generalized rate equation for single-substrate enzyme catalyzed reactions.

    abstract：:The most widely used rate expression for single-substrate enzyme catalyzed reactions, namely the Michaelis-Menten kinetics is based upon the assumption that enzyme concentration is in excess of the substrate in the medium and the rate is mainly limited by the substrate concentration according to saturation kinetics. H...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.02.155

    authors： Kargi F

    更新日期：2009-04-24 00:00:00

  • Chemical induction of cellular antioxidants affords marked protection against oxidative injury in vascular smooth muscle cells.

    abstract：:Extensive evidence suggests that reactive oxygen species are critically involved in the pathogenesis of cardiovascular diseases, such as atherosclerosis and myocardial ischemia-reperfusion injury. Consistent with this concept, administration of exogenous antioxidants has been shown to be protective against oxidative c...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2002.6614

    authors： Cao Z,Li Y

    更新日期：2002-03-22 00:00:00

  • The stimulation of prostaglandin production by transforming growth factor-alpha and 12-O-tetradecanoyl-phorbol-13-acetate or 1-oleoyl-2-acetyl-glycerol is synergistic.

    abstract：:Stimulation of prostaglandin production in C-9 rat liver cells by transforming growth factor (TGF)-alpha was synergistic with that of the tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA). TGF-alpha and TPA synergized the release of radiolabelled compounds from [3H]arachidonic acid prelabelled C-9 cells. 1-Ol...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)90389-4

    authors： Levine L,Xiao DM,Worth N,Lilley WE

    更新日期：1985-07-16 00:00:00

  • Potentiation by chronic ethanol treatment of the mitochondrial permeability transition.

    abstract：:Mitochondria isolated from rats chronically fed ethanol were more sensitive to induction of the mitochondrial permeability transition (MPT) by a variety of agents than mitochondria isolated from isocalorically matched controls. The agents utilized have been implicated in both necrotic (Ca(2+)) and apoptotic (ceramide,...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1696

    authors： Pastorino JG,Marcineviciute A,Cahill A,Hoek JB

    更新日期：1999-11-19 00:00:00