S100 to receptor for advanced glycation end-products binding assay: looking for inhibitors.

abstract：BACKGROUND:X-linked hypophosphatemia (XLH), the most common form of inherited rickets, is a dominant disorder that is characterized by renal phosphate wasting with hypophosphatemia, abnormal bone mineralization, short stature, and rachitic manifestations. The related gene with inactivating mutations associated with XLH...

journal_title：Biochemical and biophysical research communications

authors： Kang QL,Xu J,Zhang Z,He JW,Lu LS,Fu WZ,Zhang ZL

更新日期：2012-07-13 00:00:00

abstract：:The 2',3'-dideoxyriboside of 2,6-diaminopurine (ddDAPR) and its 2',3'-didehydro derivative (ddeDAPR) are poor substrates for adenosine deaminase (ADA) but potent inhibitors of the enzyme. Their Km values for ADA are of the same order of magnitude as those of the natural adenosine (Ado) and 2'-deoxyadenosine (dAdo), bu...

journal_title：Biochemical and biophysical research communications

authors： Balzarini J,Robins MJ,Zou RM,Herdewijn P,De Clercq E

更新日期：1987-05-29 00:00:00

abstract：:Matrix vesicles (MVs) are cell-derived membranous entities crucial for mineral formation in the extracellular matrix. One of the dominant groups of constitutive proteins present in MVs, recognised as regulators of mineralization in norm and pathology, are annexins. In this report, besides the annexins already describe...

journal_title：Biochemical and biophysical research communications

authors： Cmoch A,Strzelecka-Kiliszek A,Palczewska M,Groves P,Pikula S

更新日期：2011-09-09 00:00:00

abstract：:Using a culture system that facilitates osteogenic differentiation of bone marrow-derived human mesenchymal stem cells, we analyzed gene-expression profiles during the mineralization process by means of a cDNA microarray system consisting of 23,040 genes. We compared expression profiles of the cells at days 3, 15, and...

journal_title：Biochemical and biophysical research communications

authors： Doi M,Nagano A,Nakamura Y

更新日期：2002-01-11 00:00:00

abstract：:The jelly coat of echinoderm eggs contains a glycoconjugate, acrosome reaction-inducing substance (ARIS), that is essential for triggering the acrosome reaction in homologous spermatozoa. In the starfish, Asterias amurensis, ARIS is a sulfated glycoprotein of an apparent molecular size of greater than 10(7). Since its...

journal_title：Biochemical and biophysical research communications

authors： Okinaga T,Ohashi Y,Hoshi M

更新日期：1992-07-15 00:00:00

abstract：:TESK1 (testis-specific protein kinase 1) is a serine/threonine kinase, with a unique structure composed of an N-terminal protein kinase domain and a C-terminal proline-rich domain. Northern blot analysis revealed that TESK1 mRNA is predominantly expressed in testicular germ cells. We present here evidence that express...

journal_title：Biochemical and biophysical research communications

authors： Toshima J,Koji T,Mizuno K

更新日期：1998-08-10 00:00:00

abstract：:IGF-I, when added to TSH-primed FRTL-5 cells, induces a long lasting Ca2+ influx and then DNA synthesis. Ca2+ channel agonist, BAYK 8644, is capable to mimic these effects on cell proliferation. We studied the effect of genistein, a specific tyrosine kinase inhibitor, on BAY K8644- or IGF-I- induced cell cycle progres...

journal_title：Biochemical and biophysical research communications

authors： Takano T,Takada K,Tada H,Nishiyama S,Amino N

更新日期：1993-02-15 00:00:00

abstract：:Lipopolysaccharide (LPS) is the main component of Gram-negative bacteria that - upon infection - activates the host immune system and is crucial in fighting pathogens as well as in the induction of sepsis. In the present study we addressed the question whether the key structural components of LPS equally take part in ...

journal_title：Biochemical and biophysical research communications

authors： Eder K,Vizler C,Kusz E,Karcagi I,Glavinas H,Balogh GE,Vigh L,Duda E,Gyorfy Z

更新日期：2009-11-06 00:00:00

abstract：:Pancreatic elastase has been implicated in the pathophysiology of severe acute pancreatitis, characterized by systemic inflammatory response, distant organ failure, and high mortality. Here we show that pancreatic elastase activates transcription factors NF-kappaB, AP-1, and NFAT in human myeloid cells (U-937 and THP-...

journal_title：Biochemical and biophysical research communications

authors： Hietaranta A,Mustonen H,Puolakkainen P,Haapiainen R,Kemppainen E

更新日期：2004-10-08 00:00:00

abstract：:Neuroblastoma LAN-5 cells exposed to retinoic acid cease to multiply and extend neurite outgrowths acquiring a neuronal phenotype. We now report that protein kinase C-theta; (PKC-theta;) isozyme is involved in this differentiation process due to the following findings: (i) PKC-theta; is expressed by LAN-5 cells as a n...

journal_title：Biochemical and biophysical research communications

authors： Sparatore B,Patrone M,Passalacqua M,Pedrazzi M,Pontremoli S,Melloni E

更新日期：2000-12-20 00:00:00

abstract：:Direct chemical acetylation of an oligonucleosomal template for bacteriophage T7 RNA polymerase is accompanied by a substantial increase in its capability to support RNA synthesis. The template was assembled from a plasmid, containing a promoter and a terminator for T7 RNA polymerase, plus one (H3-H4)2 tetramer and tw...

journal_title：Biochemical and biophysical research communications

authors： Hernández F,Puerta C,López-Alarcón L,Palacián E

更新日期：1995-08-04 00:00:00

abstract：:The reactivity of human thioredoxin (HTR) was tested in several reactions. HTR was as efficient as E. coli or plant and algal thioredoxins when assayed with E. coli ribonucleotide reductase or for the reduction of insulin. On the other hand, HTR was poorly reduced by NADPH and the E. coli flavoenzyme NADPH thioredoxin...

journal_title：Biochemical and biophysical research communications

authors： Jacquot JP,de Lamotte F,Fontecave M,Schürmann P,Decottignies P,Miginiac-Maslow M,Wollman E

更新日期：1990-12-31 00:00:00

abstract：:Cancer cells show characteristic telomere dynamics. Their chromosomes usually have short telomeres and a high telomerase activity. The "telomere crisis model" proposed here suggests that these unique telomeric features are responsible for the progression of cancer. ...

journal_title：Biochemical and biophysical research communications

authors： Ishikawa F

更新日期：1997-01-03 00:00:00

abstract：:A polymerizable gel superaggregate has been formed from low concentrations (6 mM) of phospholipid mixtures of polymerizable 1,2-bis(tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine and 1,2-bis(dinonoyl)-sn-glycero-3-phosphocholine. Transmission electron microscopy (TEM) revealed that the superaggregate structure con...

journal_title：Biochemical and biophysical research communications

authors： Markowitz MA,Singh A,Chang EL

更新日期：1994-08-30 00:00:00

abstract：:Treatment of 8-Br-cAMP promotes neurite outgrowth and neuronal differentiation in N1E115 mouse neuroblastoma cells. Prior or simultaneous treatment of PMA blocks 8-Br-cAMP-mediated neurite outgrowth. Phosphorylation of cellular proteins during these treatments was examined in a permeabilized cell system. While PMA pro...

journal_title：Biochemical and biophysical research communications

authors： Li H,Chen HC,Huang FL

更新日期：1995-12-05 00:00:00

abstract：:The stability of mRNA influences the dynamics of gene expression. The CCR4-NOT complex, the major deadenylase in mammalian cells, shortens the mRNA poly(A) tail and contributes to the destabilization of mRNAs. The CCR4-NOT complex plays pivotal roles in various physiological functions, including cell proliferation, ap...

journal_title：Biochemical and biophysical research communications

authors： Takahashi A,Kikuguchi C,Morita M,Shimodaira T,Tokai-Nishizumi N,Yokoyama K,Ohsugi M,Suzuki T,Yamamoto T

更新日期：2012-03-09 00:00:00

abstract：:Aminopeptidase B (Ap-B) is a Zn2+-aminopeptidase of the M1 family which is implicated, in conjunction with the nardilysin endoprotease, in the generation of miniglucagon, a peptide involved in the maintenance of glucose homeostasis. Other in vivo physiological roles have been established for this vertebrate enzyme, su...

journal_title：Biochemical and biophysical research communications

authors： Cadel S,Darmon C,Désert A,Mahbouli M,Piesse C,Ghélis T,Lafont R,Foulon T

更新日期：2019-05-14 00:00:00

abstract：:Syntaxin is one of the proteins involved in the exocytotic event through sequential binding to specific proteins, including SNAP25 and synaptobrevin. In a previous work in digitonin-permeabilized beta cells, we characterized the functional role of two segments: synA and synB of the H3 domain of syntaxin. As a continua...

journal_title：Biochemical and biophysical research communications

authors： Martin F,Salinas E,Barahona F,Vázquez J,Soria B,Reig JA

更新日期：1998-07-09 00:00:00

abstract：:The major bovine seminal plasma protein, PDC-109, binds to choline phospholipids of the sperm plasma membrane and induces an efflux of cholesterol and choline phospholipids (cholesterol efflux), which is crucial for sperm capacitation. PDC-109 also exhibits chaperone-like activity and protects target proteins against ...

journal_title：Biochemical and biophysical research communications

authors： Singh BP,Saha I,Nandi I,Swamy MJ

更新日期：2017-12-02 00:00:00

abstract：:Chondroitin sulfate proteoglycan 4 (CSPG4), a transmembrane proteoglycan originally identified in melanoma cells, has been reported to be expressed in breast cancer cells. This study was performed to examine the expression and significance of CSPG4 in a cohort of breast cancer patients. Immunohistochemical analysis of...

journal_title：Biochemical and biophysical research communications

authors： Hsu NC,Nien PY,Yokoyama KK,Chu PY,Hou MF

更新日期：2013-11-15 00:00:00

abstract：:Enzyme-linked immunosorbent assays (ELISA) of somatic (cs) and testicular (ct) cytochromes of rat, mouse, rabbit, and beef with rabbit anti-rat cyt ct antibody exhibited two different antigenic profiles, indicating the presence of two different antigenic structure between cyt ct and cs. SDS-polyacrylamide gel electrop...

journal_title：Biochemical and biophysical research communications

authors： Kim IC,Sabourin CL

更新日期：1986-11-26 00:00:00

abstract：:Polyclonal antibodies have been obtained against a synthetic dodecapeptide identical to the aminoacid sequence 120-131 DSPIKDGVWPPE (inferred from its DNA sequence) of Paracoccus denitrificans cytochrome c oxidase subunit III. The antibodies had a titer higher than 1:10000 when tested against the antigen. These antibo...

journal_title：Biochemical and biophysical research communications

authors： Müller M,Labonia N,Azzi A

更新日期：1988-08-15 00:00:00

abstract：:The ability of tissue plasminogen activator (tPA) to induce human umbilical vein endothelial (HUVE) cell migration was studied using an in vitro, serum-free wound assay system. At pharmacological doses, tPA stimulated HUVE cell migration dose-dependently. Treatment of cells with epsilon amino caproic acid (EACA) to de...

journal_title：Biochemical and biophysical research communications

authors： Inyang AL,Tobelem G

更新日期：1990-09-28 00:00:00

abstract：:Cultured bovine pulmonary artery endothelial cells contain a second peptidyl dipeptidase, distinct from angiotensin-converting enzyme, present in an inactive form associated with a non-dialyzable inhibitor. Partial purification by glycine affinity chromatography separates enzyme from inhibitor to yield a preparation w...

journal_title：Biochemical and biophysical research communications

authors： Lanzillo JJ,Dasarathy Y,Stevens J,Fanburg BL

更新日期：1986-01-29 00:00:00

abstract：:Available evidence suggests that insulin-like growth factor I receptor (IGF-IR) expression leads to increased cellular radioresistance. The most direct explanation of these findings predicts that IGF-IR is the source of survival signals in resistant cells. Mutational analysis revealed that protein truncated at amino a...

journal_title：Biochemical and biophysical research communications

authors： Yu D,Shibuya H,Miura M

更新日期：2003-11-07 00:00:00

abstract：:A mutant of Photinus pyralis luciferase in which all four native cysteine residues are converted to serines retains about 10% of wild-type activity. This mutant should prove useful as a starting point for the introduction of biophysical probes of conformational changes associated with enzyme function. The activities o...

journal_title：Biochemical and biophysical research communications

authors： Kumita JR,Jain L,Safroneeva E,Woolley GA

更新日期：2000-01-07 00:00:00

abstract：:HERC2 is a giant protein with E3 ubiquitin ligase activity and other known and suspected functions. Mutations of HERC2 are implicated in the pathogenesis of various cancers and result in severe neurological conditions in Herc2-mutant mice. Recently, a pleotropic autosomal recessive HERC2-associated syndrome of intelle...

journal_title：Biochemical and biophysical research communications

authors： Abraham JR,Barnard J,Wang H,Noritz GH,Yeganeh M,Buhas D,Natowicz MR

更新日期：2019-04-30 00:00:00

abstract：:Cellular invasion of extracellular matrix (ECM) occurs during normal and pathological settings. For cells to invade, they must adhere to the underlying substratum, break down barrier molecules, and detach from the substratum prior to migrating through the ECM. We previously demonstrated that incubation under reduced o...

journal_title：Biochemical and biophysical research communications

authors： Lash GE,Fitzpatrick TE,Graham CH

更新日期：2001-09-28 00:00:00

abstract：:Treatment of rat basophilic leukemia cell line (2H3) with interferon-alpha significantly increased intracellular histamine levels. On the other hand, the histidine content was decreased reciprocally by interferon in a dose-dependent manner. Concomitantly, the activity of histidine decarboxylase, the enzyme responsible...

journal_title：Biochemical and biophysical research communications

authors： Kito T,Kubota M,Takimoto T,Akiyama Y,Heike T,Mikawa H

更新日期：1989-01-16 00:00:00

abstract：:Ultrasound (US) has emerged as a promising noninvasive modality for neuromodulation. Despite previous evidence that US may mediate cellular response by activating mechanosensitive ion channels embedded in the cell membrane, the underlying mechanism is not well understood. In this work, we developed a vertically deploy...

journal_title：Biochemical and biophysical research communications

authors： Liao D,Li F,Lu D,Zhong P

更新日期：2019-10-20 00:00:00