Abstract:
:Pulsed-field gel electrophoresis and flow dot-blot analysis have been used to construct a physical map of the q32-q34 region of chromosome 9, where one of the loci responsible for tuberous sclerosis (TSC1) has been mapped by genetic linkage. Five linked groups of markers have been defined by pulsed-field gel electrophoresis. The orientation of these groups and the order of markers within them were determined by hybridization to flow-sorted dot blots derived from a panel of cell lines of chromosome 9 translocations to place probes proximal or distal to each breakpoint. The local map order in 9q32-q34 derived by application of this combination of techniques is as follows: centromere-ALAD-1.3 Mb-ORM/20 kbD9S16-GSN-250 kb-C5-HXB-1.9 Mb-D9S21-AK1-1.4 Mb-SPTAN1-ASS-800 kb-ABL-2 Mb-D9S10/350 Kb/DBH-telomere.
journal_name
Genomicsjournal_title
Genomicsauthors
Harris RM,Carter NP,Griffiths B,Goudie D,Hampson RM,Yates JR,Affara NA,Ferguson-Smith MAdoi
10.1006/geno.1993.1056subject
Has Abstractpub_date
1993-02-01 00:00:00pages
265-74issue
2eissn
0888-7543issn
1089-8646pii
S0888-7543(83)71056-6journal_volume
15pub_type
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