Abstract:
:The Xist sequence has been proposed as a potential candidate for the X-inactivation center based both on its localization within the candidate region for the X-inactivation center in man and mouse and on its unique pattern of expression from the inactive X chromosome. We have cloned 550 kb of DNA surrounding the mouse Xist sequence in contiguously overlapping YAC clones and have developed a long-range restriction map that spans almost 1 Mb of this region and includes this YAC contig. The detailed restriction map we have established provides a framework for the identification of expressed sequences other than Xist that may equally exhibit unusual expression characteristics associated with X inactivation. The presence of possible structural or methylation differences within this region between the active and inactive X chromosomes has been investigated through comparative analysis of male and female genomic DNA, and we report here the identification of certain CpG-containing restriction sites around Xist that have an interesting differential methylation status on the inactive and active X chromosomes.
journal_name
Genomicsjournal_title
Genomicsauthors
Heard E,Simmler MC,Larin Z,Rougeulle C,Courtier B,Lehrach H,Avner Pdoi
10.1006/geno.1993.1108subject
Has Abstractpub_date
1993-03-01 00:00:00pages
559-69issue
3eissn
0888-7543issn
1089-8646pii
S0888-7543(83)71108-0journal_volume
15pub_type
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