Transcription starting from an alternative promoter leads to the expression of the human ABO histo-blood group antigen.

Abstract:

BACKGROUND:Using the 5'-rapid amplification of cDNA ends technique with the ex vivo culture of AC133-CD34+ cells, a transcription start site was recently identified approximately 0.7 kb upstream from the transcription start sites previously determined. The transcripts from the alternative starting exon 1a were demonstrated in the cells of both erythroid and epithelial lineages. Because the nucleotide sequence of exon 1a does not contain an ATG codon, we examined whether transcription starting from exon 1a leads to production of a functional glycosyltransferase. STUDY DESIGN AND METHODS:Stable transfection experiments into the human gastric cancer MKN28 cells were performed using the various A transferase expression plasmids. RESULTS:Large amounts of A antigens were demonstrated on the cells transfected with the A transferase expression plasmid containing the entire cDNA from exon 1a or the 5'-truncated cDNA leading to the production of the N-truncated protein with deletion of the cytoplasmic tail and a portion of the transmembrane domain. However, negligible amounts of A antigens were observed on the cells transfected with the A transferase expression plasmids containing the 5'-truncated cDNA leading to the production of the N-truncated proteins without the cytoplasmic tail and the transmembrane domain. CONCLUSION:This study suggests that a functional A transferase could be produced by the transcription from exon 1a.

journal_name

Transfusion

journal_title

Transfusion

authors

Hata Y,Kominato Y,Takizawa H,Tabata S,Michino J,Nishino K,Yasumura S,Yamamoto F

doi

10.1046/j.1537-2995.2003.00382.x

subject

Has Abstract

pub_date

2003-05-01 00:00:00

pages

656-62

issue

5

eissn

0041-1132

issn

1537-2995

pii

382

journal_volume

43

pub_type

杂志文章
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    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1977.17578014580.x

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    authors: Busch MP,Tobler L,Quan S,Wilber JC,Johnson P,Polito A,Steane E,Zola A,Bahl C,Nelles M

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15463

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15621

    authors: Nixon S,Lieberman L,Atenafu EG,Doherty M,Murray C,Wolfe A,Brandys D,Rowland S,Kwan BY,Yu E,Lechner B,Maze D

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    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1982.22182154221.x

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  • Hematology patterns after hemoglobin-based oxygen carrier resuscitation from severe controlled hemorrhage with prolonged delayed definitive care.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2007.01435.x

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  • Impact of cord blood banking technologies on clinical outcome: a Eurocord/Cord Blood Committee (CTIWP), European Society for Blood and Marrow Transplantation and NetCord retrospective analysis.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.13661

    authors: Saccardi R,Tucunduva L,Ruggeri A,Ionescu I,Koegler G,Querol S,Grazzini G,Lecchi L,Nanni Costa A,Navarrete C,Pouthiers F,Larghero J,Regan D,Freeman T,Bittencourt H,Kenzey C,Labopin M,Baudoux E,Rocha V,Gluckman E

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    pub_type: 杂志文章

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    pub_type: 杂志文章

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    更新日期:1981-11-01 00:00:00

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    journal_title:Transfusion

    pub_type: 杂志文章

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    journal_title:Transfusion

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1111/trf.15786

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  • In vivo red cell destruction by anti-Lu6.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1990.30390194349.x

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    更新日期:1990-03-01 00:00:00

  • Immunochemical studies on the differential binding properties of two monoclonal antibodies reacting with Tn red cells.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1991.31291142945.x

    authors: King MJ,Parsons SF,Wu AM,Jones N

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  • The minimum number of cord blood units needed for Koreans is 51,000.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12284

    authors: Yoon JH,Oh S,Shin S,Park JS,Roh EY,Song EY,Park MH,Han KS

    更新日期:2014-03-01 00:00:00

  • Influence of climate on donor deferrals.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1988.28689059031.x

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    更新日期:1988-11-01 00:00:00

  • Dimethyl sulfoxide: effects on function of fresh platelets and on the viability of platelets in storage.

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    journal_title:Transfusion

    pub_type: 杂志文章

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  • NBTS/BRIC 8. A monoclonal anti-C3d antibody.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1985.25385219912.x

    authors: Holt PD,Donaldson C,Judson PA,Johnson P,Parsons SF,Anstee DJ

    更新日期:1985-05-01 00:00:00

  • Extension of platelet shelf life with an improved bacterial testing algorithm.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.16112

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.13622

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    pub_type: 杂志文章

    doi:10.1111/trf.12853

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    journal_title:Transfusion

    pub_type: 杂志文章

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    pub_type: 杂志文章

    doi:10.1111/trf.12263

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    journal_title:Transfusion

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验

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    更新日期:1996-01-01 00:00:00

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    authors: O'Connell B,Lee EJ,Schiffer CA

    更新日期:1988-01-01 00:00:00

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    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2010.02706.x

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    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1997.37897424398.x

    authors: Fritsch G,Printz D,Stimpfl M,Dworzak MN,Witt V,Pötschger U,Buchinger P

    更新日期:1997-08-01 00:00:00