Abstract:
BACKGROUND:Radiation and filtration have achieved satisfactory results in inactivation or removal of tumor cells mixed in salvage blood, but some drawbacks remain. This study evaluated the inactivation on HCT116 cells mixed in simulative salvage blood by riboflavin photochemical treatment. METHODS:HCT116 cells were added to the whole blood to simulate contaminated salvaged blood. The mixed blood was added with riboflavin of 50 μmol/L final concentration and illuminated by ultraviolet light. The samples were divided into control group and Experimental Groups 1 (18 J/cm2 ), 2 (23.4 J/cm2 ), and 3 (28.8 J/cm2 ). An autotransfusion system (Cell Saver Elite, Haemonetics) was used to simulate the intraoperative blood salvage procedure to deal with whole blood. The apoptosis rate and tumorigenicity of HCT116 cells and the superimposed damage to red blood cells (RBCs) were evaluated. RESULTS:The apoptosis rates of HCT116 in Experimental Groups 1, 2, and 3 were much higher than that in the control group. Tumor growth was found in the control group, but no tumor growth was found in the three experimental groups. The hemolysis rates in the three experimental groups were significantly higher than that in the control group, but much lower than the quality standard of RBCs at the end of preservation. The concentration of adenosine triphosphate in RBCs was comparable in the control and experimental groups. CONCLUSION:Riboflavin at a 50 μmol/L final concentration and 18 J/cm2 ultraviolet illumination can effectively inactivate HCT116 cells in salvaged blood, with minimum damage to the structure and function of RBCs, and the main quality indexes of salvaged RBCs were within the standard range.
journal_name
Transfusionjournal_title
Transfusionauthors
Yu Y,Yang L,He C,Tai S,Ma C,Yang T,Wang Ddoi
10.1111/trf.15499subject
Has Abstractpub_date
2019-10-01 00:00:00pages
3205-3213issue
10eissn
0041-1132issn
1537-2995journal_volume
59pub_type
杂志文章相关文献
TRANSFUSION文献大全abstract::A 24-year-old, blood-group O Polynesian woman with multiple red blood cell antibodies received a group A, HLA-compatible, sex-matched bone marrow transplantation as treatment for severe aplastic anemia. High retransplantation titers of ABO isohemagglutinins were successfully reduced by repeated plasmaphereses. Despite...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1982.22182154221.x
更新日期:1982-01-01 00:00:00
abstract:BACKGROUND:The purpose of this study was to develop human immunodeficiency virus (HIV) infection donation deferral criteria for blood donors in an HIV-epidemic area of northern Thailand, where the predominant means of transmission of HIV is through heterosexual contact. STUDY DESIGN AND METHODS:In a preliminary study,...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1996.36396182143.x
更新日期:1996-03-01 00:00:00
abstract::Anti-D sensitized red cells were converted to stroma, either by digitonin or hypotonic lysis, and eluates prepared by the modified rapid acid-stromal method. SDS-PAGE and both rocket- and crossed-immunoelectrophoresis showed that, of the total protein content of the eluates, 11 to 25 percent was IgG, 18 to 45 percent ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1983.23383224906.x
更新日期:1983-05-01 00:00:00
abstract:BACKGROUND:The aim was to assess iron status and dietary iron intake in a sample of premenopausal female regular and new blood donors. STUDY DESIGN AND METHODS:Premenopausal women blood donors were invited to participate. Blood samples were analyzed for serum ferritin and hemoglobin. An iron checklist assessed dietary...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12347
更新日期:2014-03-01 00:00:00
abstract:BACKGROUND:Direct oral anticoagulants (DOACs) are effective and safe for prophylaxis and treatment of thromboembolic phenomena. However, managing DOACs during bleeding emergencies is challenging. A systematic review and meta-analysis was conducted on studies addressing efficacy and safety of the drugs used for reversal...
journal_title:Transfusion
pub_type: 杂志文章,meta分析,评审
doi:10.1111/trf.14096
更新日期:2017-07-01 00:00:00
abstract:BACKGROUND:Loss of blood group ABO antigens on red blood cells (RBCs) is well known in patients with leukemias, and such decreased ABO expression has been reported to be strongly associated with hypermethylation of the ABO promoter. We investigated the underlying mechanism responsible for A-antigen reduction on RBCs in...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15628
更新日期:2020-01-01 00:00:00
abstract:BACKGROUND:The inventory size for cord blood (CB) depends on the ethnic diversity of human leukocyte antigen (HLA) and the size estimation is important for public health in each ethnicity. STUDY DESIGN AND METHODS:We estimate the CB inventory size in Koreans with stored CB units (CBUs) and patients who underwent allog...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.12284
更新日期:2014-03-01 00:00:00
abstract:BACKGROUND:Recent studies conducted in developed countries described hepatitis E virus (HEV) as an emerging infectious threat to blood safety. However, data on HEV among blood donors from southeast Asia are lacking. STUDY DESIGN AND METHODS:Between July and August 2014, we assessed the presence of HEV immunoglobulin (...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.13731
更新日期:2016-10-01 00:00:00
abstract:BACKGROUND:Umbilical cord blood (UCB) transplantation is becoming more widely used, yet ethical and policy issues regarding consent and health history persist. Whereas most UCB banks do not require paternal consent or paternal health history (PHH), both are obtained at this institution whenever possible. This study ass...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.2002.00188.x
更新日期:2002-10-01 00:00:00
abstract:BACKGROUND:Transfusion-transmitted babesiosis (TTB) has been rapidly increasing in incidence since the beginning of the 21st century. Asymptomatic individuals with Babesia infection are able to donate blood in the United States because of the lack of specific blood donation testing. Blood products collected in Babesia-...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.14246
更新日期:2017-10-01 00:00:00
abstract:BACKGROUND:Evaluation of hemostasis in bleeding patients requires both accuracy and speed. STUDY DESIGN AND METHODS:As an alternative to point-of-care testing, we developed an emergency hemorrhage panel (EHP: prothrombin time [PT], fibrinogen, platelet count, hematocrit) for use in making transfusion decisions on blee...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2010.02753.x
更新日期:2010-12-01 00:00:00
abstract::Red cells from a Mexican blood donor had depressed H, IF, ID, i, Sda, Sdx, D, LW, Dib, Vel and JMH antigens. A wide range of other blood group antigens had normal activity. The unusual red cell phenotype may be caused by a membrane anomaly that alters orientation of certain cell surface components. The specificities o...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1983.23183147309.x
更新日期:1983-01-01 00:00:00
abstract:BACKGROUND:Absolute counts of CD4+ T-lymphocytes are used in the management of patients with human immunodeficiency virus infection. Low absolute counts of CD3+CD4+ cells have also been observed in healthy people--a phenomenon called idiopathic CD4 lymphocytopenia. It is common practice for normal ranges for lymphocyte...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1996.36696269517.x
更新日期:1996-06-01 00:00:00
abstract:BACKGROUND:Screening blood units for hepatitis C virus (HCV) with nucleic acid testing (NAT) reduces the risk associated with the long "window period" (8-9 weeks) after HCV infection. The feasibility of adding the HCV core antigen assay in pools to the existing anti-HCV individual screening was examined as an alternati...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2006.00972.x
更新日期:2006-10-01 00:00:00
abstract::The studies reported in this monograph were performed between 1972 and 2002 when it was possible to study healthy male and female baboons. A colony of baboons was maintained for 30 years without any adverse events observed in these baboons in the numerous studies that were performed. These protocols were reviewed and ...
journal_title:Transfusion
pub_type: 杂志文章,评审
doi:10.1111/j.1537-2995.2006.00922.x
更新日期:2006-08-01 00:00:00
abstract:BACKGROUND:Recombinant activated factor VII (rFVIIa) is being tested to improve hemostasis in a variety of bleeding disorders. Clinical indications and efficacy are still being evaluated for this product. CASE REPORT:Over a 17-month period, rFVIIa was used to treat central nervous system hemorrhage in three patients w...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2004.04080.x
更新日期:2004-11-01 00:00:00
abstract::This paper presents recent data from quantitative and thermodynamic investigations on A and B erythrocyte antigens. In regard to weak A phenotypes on the basis of quantitative data, practically no gap is observed from the weakest Aend to the strongest A3. Weak B phenotypes give a similar distribution. However, a more ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1976.16677060242.x
更新日期:1976-11-01 00:00:00
abstract:BACKGROUND:Current transfusion-related acute lung injury reduction strategies include avoiding transfusion of plasma products collected from female donors or female donors that have been pregnant to reduce transfusion of plasma-containing HLA antibodies. Such a policy considerably decreases the number of donors availab...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2009.02198.x
更新日期:2009-08-01 00:00:00
abstract:BACKGROUND:Safe, accurate methods permitting simultaneous and/or repeated measurement of red blood cell (RBC) survival (RCS) are important to investigate pathophysiology and therapy of anemia. Methods using chromium 51 ((51) Cr)-labeled RBCs are unacceptable for infants, children, and pregnant women. We report RCS meas...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2010.02926.x
更新日期:2011-05-01 00:00:00
abstract:BACKGROUND:Anti-Tc(a) detects a high-incidence antigen in the Cromer blood group system. Cromer system antibodies have not usually been associated with hemolytic transfusion reactions or hemolytic disease of the newborn. CASE REPORT:Anti-Tc(a) (initially identified in the patient's serum in 1982) was not detected when...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1999.39090948.x
更新日期:1999-09-01 00:00:00
abstract:BACKGROUND:Pigs are a potential source of red blood cells (RBCs) for transfusion into humans, but the presence of galactose-alpha1,3-galactose (Gal) epitopes on their surface, against which humans have anti-Gal, has been perceived as a major barrier. alpha1,3-Galactosyltransferase gene-knockout pigs, which do not expre...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2004.04002.x
更新日期:2004-07-01 00:00:00
abstract:BACKGROUND:Passenger lymphocyte-mediated graft-versus-host disease (GVHD) in solid organ transplantation (SOT-GVHD) is considered a rare complication, particularly among recipients of lung allografts. The risk of transfusion-associated GVHD (TA-GVHD) in solid organ transplants is also considered rare. The suspicion of ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2008.01835.x
更新日期:2008-10-01 00:00:00
abstract:BACKGROUND:Vitamin K is reported to begin reversing warfarin within 6 to 12 hours, but this may occur sooner. We sought to determine the rate of international normalized ratio (INR) reversal following vitamin K and relationships with dose, route, and baseline INR. METHODS:We evaluated adult patients receiving vitamin ...
journal_title:Transfusion
pub_type: 临床试验,杂志文章
doi:10.1111/trf.15146
更新日期:2019-04-01 00:00:00
abstract:BACKGROUND:Immune thrombocytopenic purpura and secondary thrombocytopenia patients treated with Rh(o)(D) immune globulin intravenous (human; anti-D IGIV) have experienced acute hemolysis, which is inconsistent with the typical presentation of extravascular hemolysis -- the presumed mechanism of action of anti-D IGIV. A...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2008.02083.x
更新日期:2009-06-01 00:00:00
abstract:INTRODUCTION:The National Healthcare Safety Network (NHSN) Hemovigilance Module (HM) collects data on the frequency, severity, and imputability of transfusion-associated adverse events. These events contribute to significant morbidity and mortality among transfusion patients. We report results from the first systematic...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/trf.15008
更新日期:2019-02-01 00:00:00
abstract::The purpose of this study was to ascertain the cause for seasonal variations of donor deferral. Deferred donors and monthly temperatures from 1981 to 1984 were tallied and analyzed. Temperatures were categorized at 10-degree intervals from 15 degrees F to 75 degrees F. Donors failing the hemoglobin screening then unde...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1988.28689059031.x
更新日期:1988-11-01 00:00:00
abstract::The vasodilative effects associated with the use of sodium acetate have been reported in the literature. Preparations of plasma protein fraction (PPF) substantially free of this vasodilator were developed. This paper presents and discusses experimental work on the improved product. ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1981.21682085754.x
更新日期:1981-11-01 00:00:00
abstract:BACKGROUND:The rare Bombay phenotype is usually due to a primary genetic defect in an alpha-2- or alpha-4-fucosyltransferase. The present study was done to investigate a patient with normal transferases, who exhibits the Bombay phenotype. CASE REPORT:Red cells of the patient, his parents, and siblings were phenotyped ...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1995.35996029164.x
更新日期:1995-09-01 00:00:00
abstract::Kell blood-group-active protein has been isolated by labeling red cell surface proteins with 125I, sensitizing intact cells with anti-K1, anti-K2, anti-K7, or anti-K22, solubilizing the cell membranes, isolating immune complexes, and separating their components by sodium dodecyl sulfate polyacrylamide gel electrophore...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1046/j.1537-2995.1984.24284173356.x
更新日期:1984-03-01 00:00:00
abstract::Retrospective studies suggest that the transfusion of older, stored red blood cells (RBCs) may be associated with increases in mortality, serious infections, multiorgan failure, thrombosis, and hospital length of stay. Our research is based on the overarching hypothesis that the adverse effects associated with transfu...
journal_title:Transfusion
pub_type: 杂志文章
doi:10.1111/j.1537-2995.2011.03096.x
更新日期:2011-04-01 00:00:00