Abstract:
:Anti-D sensitized red cells were converted to stroma, either by digitonin or hypotonic lysis, and eluates prepared by the modified rapid acid-stromal method. SDS-PAGE and both rocket- and crossed-immunoelectrophoresis showed that, of the total protein content of the eluates, 11 to 25 percent was IgG, 18 to 45 percent was globin, and 34 to 71 percent was red cell membrane protein. Eluates were free of albumin, transferrin, and other non-IgG serum proteins; they did not contain proteolytic activity, and eluates were noninhibitory to the growth of committed erythroid progenitors. The rapid acid-stromal method produced high-titered blood group antibody eluates free of contaminating immune or nonimmune activity which were suitable for assay of blood group antigen expression by hemopoietic stem cells and committed progenitors.
journal_name
Transfusionjournal_title
Transfusionauthors
Rearden A,Chiu P,Elmajian Ddoi
10.1046/j.1537-2995.1983.23383224906.xsubject
Has Abstractpub_date
1983-05-01 00:00:00pages
248-51issue
3eissn
0041-1132issn
1537-2995journal_volume
23pub_type
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