Identification and quantitation of the protein content of blood group antibody eluates prepared by the rapid acid-stromal method. Use in committed erythroid progenitor cell assays.

Abstract:

:Anti-D sensitized red cells were converted to stroma, either by digitonin or hypotonic lysis, and eluates prepared by the modified rapid acid-stromal method. SDS-PAGE and both rocket- and crossed-immunoelectrophoresis showed that, of the total protein content of the eluates, 11 to 25 percent was IgG, 18 to 45 percent was globin, and 34 to 71 percent was red cell membrane protein. Eluates were free of albumin, transferrin, and other non-IgG serum proteins; they did not contain proteolytic activity, and eluates were noninhibitory to the growth of committed erythroid progenitors. The rapid acid-stromal method produced high-titered blood group antibody eluates free of contaminating immune or nonimmune activity which were suitable for assay of blood group antigen expression by hemopoietic stem cells and committed progenitors.

journal_name

Transfusion

journal_title

Transfusion

authors

Rearden A,Chiu P,Elmajian D

doi

10.1046/j.1537-2995.1983.23383224906.x

subject

Has Abstract

pub_date

1983-05-01 00:00:00

pages

248-51

issue

3

eissn

0041-1132

issn

1537-2995

journal_volume

23

pub_type

杂志文章
  • Crossmatch compatible blood for patients with anti-P1.

    abstract::Anti-P1 is a cold-reactive antibody often found in the serum of P2 individuals. For the past two years our reference laboratory has encouraged the use of crossmatch compatible blood for patients with Anti-P1 rather than the use of reagent-typed P1 negative units. The authors recently surveyed those hospitals that refe...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1978.18679077956.x

    authors: Cronin CA,Pohl BA,Miller WV

    更新日期:1978-11-01 00:00:00

  • Assessment of cord blood hematopoietic cell parameters before and after cryopreservation.

    abstract:BACKGROUND:The testing of cord blood (CB) progenitor and stem cell units for transplantation suitability involves enumeration of total nucleated cells before freezing. CD34+ cell counts may also be a means of determining suitability. Studies have been conducted to evaluate how specific storage conditions influence cell...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2007.01327.x

    authors: Kurtz J,Seetharaman S,Greco N,Moroff G

    更新日期:2007-09-01 00:00:00

  • The evolving role of plerixafor in hematopoietic progenitor cell mobilization.

    abstract::The introduction of plerixafor as a peripheral blood stem cell mobilization agent has allowed more patients with multiple myeloma, non-Hodgkin's lymphoma, and Hodgkin's disease to mobilize sufficient hematopoietic progenitor cells (HPCs) to proceed to autologous transplantation. Because of the high cost of plerixafor,...

    journal_title:Transfusion

    pub_type: 杂志文章,评审

    doi:10.1111/trf.12102

    authors: Tanhehco YC,Vogl DT,Stadtmauer EA,O'Doherty U

    更新日期:2013-10-01 00:00:00

  • Effects of new brochures on blood donor recruitment and retention.

    abstract:BACKGROUND:Currently, donors may arrive at blood collection sites without prior knowledge of eligibility and deferral criteria. STUDY DESIGN AND METHODS:The effects of distributing newly developed recruitment brochures 2 weeks in advance of blood drives and the provision of brochures on temporary deferral at the takin...

    journal_title:Transfusion

    pub_type: 临床试验,杂志文章

    doi:10.1046/j.1537-2995.1994.34794330012.x

    authors: Gimble JG,Kline L,Makris N,Muenz LR,Friedman LI

    更新日期:1994-07-01 00:00:00

  • Impact of cord blood banking technologies on clinical outcome: a Eurocord/Cord Blood Committee (CTIWP), European Society for Blood and Marrow Transplantation and NetCord retrospective analysis.

    abstract:BACKGROUND:Techniques for banking cord blood units (CBUs) as source for hematopoietic stem cell transplantation have been developed over the past 20 years, aimed to improve laboratory efficiency without altering the biologic properties of the graft. A large-scale, registry-based assessment of the impact of the banking ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.13661

    authors: Saccardi R,Tucunduva L,Ruggeri A,Ionescu I,Koegler G,Querol S,Grazzini G,Lecchi L,Nanni Costa A,Navarrete C,Pouthiers F,Larghero J,Regan D,Freeman T,Bittencourt H,Kenzey C,Labopin M,Baudoux E,Rocha V,Gluckman E

    更新日期:2016-08-01 00:00:00

  • Platelet storage in a plasma-free medium.

    abstract::Currently, platelet concentrates are stored in 50 to 60 ml of plasma. A major drawback to storage in plasma is the considerable loss of platelet function which occurs during storage. A modified Tyrodes medium has been developed for storage of platelets. A comparison between platelet concentrates stored in this medium ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1985.25686071429.x

    authors: Rock G,Swenson SD,Adams GA

    更新日期:1985-11-01 00:00:00

  • A novel bioreactor and culture method drives high yields of platelets from stem cells.

    abstract:BACKGROUND:Platelet (PLT) transfusion is the primary treatment for thrombocytopenia. PLTs are obtained exclusively from volunteer donors, and the PLT product has only a 5-day shelf life, which can limit supply and result in PLT shortages. PLTs derived from stem cells could help to fill this clinical need. However, curr...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.13375

    authors: Avanzi MP,Oluwadara OE,Cushing MM,Mitchell ML,Fischer S,Mitchell WB

    更新日期:2016-01-01 00:00:00

  • A revised classification scheme for acute transfusion reactions.

    abstract:BACKGROUND:Although the standard classification system for acute transfusion reactions adequately describes the general features associated with the various types of reactions, it was not designed to provide strict criteria for diagnosis and classification. Consequently, its use to classify individual reactions can res...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2007.01163.x

    authors: Sanders RP,Geiger TL,Heddle N,Pui CH,Howard SC

    更新日期:2007-04-01 00:00:00

  • Transfusion of murine red blood cells expressing the human KEL glycoprotein induces clinically significant alloantibodies.

    abstract:BACKGROUND:Red blood cell (RBC) alloantibodies to nonself antigens may develop after transfusion or pregnancy, leading to morbidity and mortality in the form of hemolytic transfusion reactions or hemolytic disease of the newborn. A better understanding of the mechanisms of RBC alloantibody induction, or strategies to m...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12217

    authors: Stowell SR,Girard-Pierce KR,Smith NH,Henry KL,Arthur CM,Zimring JC,Hendrickson JE

    更新日期:2014-01-01 00:00:00

  • A new radioimmunoassay for the detection of small amounts of white cells and platelets in red cell concentrates: implications for blood transfusion.

    abstract::In the procedure for quality control of red cell concentrates, made white cell (WBC)-poor by filtration, the particle-counting technique was found to be insufficiently sensitive in detecting the remaining WBCs and platelets. Therefore, direct radioimmunoassays were developed using murine monoclonal antibodies specific...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1990.30290162896.x

    authors: Webster MR,Prins HK,Huisman JG

    更新日期:1990-02-01 00:00:00

  • Evaluation of riboflavin photochemical treatment for inactivation of HCT116 tumor cells mixed in simulative intraoperative salvage blood.

    abstract:BACKGROUND:Radiation and filtration have achieved satisfactory results in inactivation or removal of tumor cells mixed in salvage blood, but some drawbacks remain. This study evaluated the inactivation on HCT116 cells mixed in simulative salvage blood by riboflavin photochemical treatment. METHODS:HCT116 cells were ad...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15499

    authors: Yu Y,Yang L,He C,Tai S,Ma C,Yang T,Wang D

    更新日期:2019-10-01 00:00:00

  • Detection of dengue, chikungunya, and Zika RNA in blood donors from Southeast Asia.

    abstract:BACKGROUND:Chikungunya (CHIKV), dengue (DENV), and Zika (ZIKV) viruses are of concern due to the potential of transfusion transmission in blood, especially in regions such as Southeast Asia where the viruses are endemic. The recent availability of nucleic acid testing (NAT) to screen blood donations on an automated pla...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.16110

    authors: Stanley J,Chongkolwatana V,Duong PT,Kitpoka P,Stramer SL,Dung NTT,Grimm KE,Pojanasingchod A,Suksomboonvong P,Galel SA

    更新日期:2020-10-07 00:00:00

  • Implementation of a script for predonation interviews: impact on human immunodeficiency virus risk in South African blood donors.

    abstract:BACKGROUND:The way in which the donor history questionnaire is conducted plays a crucial role in the self-disclosure of behavioral risk factors for human immunodeficiency virus (HIV) infection by prospective donors. The South African National Blood Service changed its policy on the process of donor assessment in May 20...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15288

    authors: Mitchel J,Custer B,Kaidarova Z,Murphy EL,van den Berg K,Recipient Epidemiology and Donor Evaluation Study-III (REDS-III) South Africa Program.

    更新日期:2019-07-01 00:00:00

  • The prevalence, immunogenicity, and evanescence of alloantibodies to MUT and Mur antigens of GP.Mur red blood cells in a Southeast Asian patient cohort.

    abstract:BACKGROUND:Antibodies to Mia , MUT, and Mur are among the most frequently identified alloantibodies in Southeast Asia. Understanding the characteristics of these antibodies in terms of induction and evanescence would aid in optimizing methods for their detection. STUDY DESIGN AND METHODS:Antibody testing results betwe...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.14538

    authors: Nadarajan VS

    更新日期:2018-05-01 00:00:00

  • Five-day storage of platelets in a non-diethylhexyl phthalate-plasticized container.

    abstract::A non-diethylhexyl phthalate (DEHP)-plasticized blood bag for 5-day storage of random-donor platelet concentrates has been developed. The plastic bag is composed of polyvinylchloride plastic with a butyryl trihexyl citrate plasticizer. The suitability of this plastic for the storage of platelet concentrates for use in...

    journal_title:Transfusion

    pub_type: 临床试验,杂志文章

    doi:10.1046/j.1537-2995.1992.32893032101.x

    authors: Snyder EL,Aster RH,Heaton A,Grode G,Napychank P,Kagen L,Jefferies LC,Hedberg S,Buchholz DH

    更新日期:1992-10-01 00:00:00

  • Real-time symptomatic case of transfusion-transmitted dengue.

    abstract:BACKGROUND:Dengue virus transmission by blood transfusion is a rarely reported event. CASE REPORT:During a dengue outbreak in São Paulo city, a regular plateletpheresis donor informed the blood bank of being diagnosed a few days after donation. The recipient was hospitalized and displayed symptoms and laboratory evide...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12944

    authors: Levi JE,Nishiya A,Félix AC,Salles NA,Sampaio LR,Hangai F,Sabino EC,Mendrone A Jr

    更新日期:2015-05-01 00:00:00

  • Dengue viremia in blood donors from Honduras, Brazil, and Australia.

    abstract:BACKGROUND:Dengue fever and hemorrhagic disease are caused by four dengue virus (DENV) serotypes (DENV-1 to -4), mosquito-borne flaviviruses with increasing incidence, and expanding global distributions. Documented transfusion transmission of West Nile virus raised concern regarding transfusion-transmitted DENV. METHO...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2008.01772.x

    authors: Linnen JM,Vinelli E,Sabino EC,Tobler LH,Hyland C,Lee TH,Kolk DP,Broulik AS,Collins CS,Lanciotti RS,Busch MP

    更新日期:2008-07-01 00:00:00

  • The impact of temporary deferral due to low hemoglobin: future return, time to return, and frequency of subsequent donation.

    abstract:BACKGROUND:This study investigated the effects of a 6-month deferral due to low hemoglobin (Hb) on the subsequent donation patterns of Australian whole blood donors. STUDY DESIGN AND METHODS:The study was a retrospective cohort study of the donation patterns of all whole blood donors deferred for low Hb during a 2-mon...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2010.02881.x

    authors: Hillgrove T,Moore V,Doherty K,Ryan P

    更新日期:2011-03-01 00:00:00

  • Hemolytic markers following the transfusion of uncrossmatched, cold-stored, low-titer, group O+ whole blood in civilian trauma patients.

    abstract:BACKGROUND:Low-titer group O whole blood (LTOWB) is increasingly being used in the civilian trauma setting, although there is a risk of hemolysis. This study evaluated the impact on hemolytic markers following the transfusion of 4 or more units of uncrossmatched LTOWB. METHODS:Civilian adult trauma patients who receiv...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15629

    authors: Harrold IM,Seheult JN,Alarcon LH,Corcos A,Sperry JL,Triulzi DJ,Yazer MH

    更新日期:2020-06-01 00:00:00

  • Selective elution of HLA antigens and beta 2-microglobulin from human platelets by chloroquine diphosphate.

    abstract::To determine whether chloroquine can specifically elute HLA antigens and beta 2-microglobulin (beta 2-M) from the platelet surface, quantitative immunofluorescence flow cytometry and monoclonal antibodies were used to show that HLA antigens and beta 2-M were proportionally eluted from the platelet surface without affe...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1988.28188127942.x

    authors: Kao KJ

    更新日期:1988-01-01 00:00:00

  • Donor body mass index is an important factor that affects peripheral blood progenitor cell yield in healthy donors after mobilization with granulocyte-colony-stimulating factor.

    abstract:BACKGROUND:The use of hematopoietic progenitor cell (HPC) transplantation has rapidly expanded in recent years. Currently, several sources of HPCs are available for transplantation including peripheral blood HPCs (PBPCs), cord blood cells, and marrow cells. Of these, PBPC collection has become the major source of HPCs....

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12238

    authors: Chen J,Burns KM,Babic A,Carrum G,Kennedy M,Segura FJ,Garcia S,Potts S,Leveque C

    更新日期:2014-01-01 00:00:00

  • Prolonged platelet preservation by transient metabolic suppression.

    abstract:BACKGROUND:In this study whether metabolic suppression can be used to preserve platelet (PLT) function during prolonged storage was investigated. STUDY DESIGN AND METHODS:Washed human PLTs were incubated without glucose and with antimycin A to block energy generation. Metabolic suppressed PLTs (MSPs) were stored for 7...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2004.04022.x

    authors: Badlou BA,Ijseldijk MJ,Smid WM,Akkerman JW

    更新日期:2005-02-01 00:00:00

  • Direct identification of Yersinia enterocolitica in blood by polymerase chain reaction amplification.

    abstract::Primers based on the nucleotide sequence of the virF gene in the pYV plasmid and the chromosomal ail gene were used in polymerase chain reaction (PCR) amplifications to directly identify Yersinia enterocolitica in blood. Approximately 500 bacteria seeded into 100 microL of blood can be extracted and amplified by PCR t...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1992.32993110759.x

    authors: Feng P,Keasler SP,Hill WE

    更新日期:1992-11-01 00:00:00

  • Effect of fresh-frozen plasma transfusion on prothrombin time and bleeding in patients with mild coagulation abnormalities.

    abstract:BACKGROUND:Fresh-frozen plasma (FFP) is frequently transfused to patients with mild prolongation of coagulation values under the assumption that FFP will correct the coagulopathy. There is little evidence to support this practice, however. To determine the effect of FFP on coagulation variables and correlation with ble...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2006.00891.x

    authors: Abdel-Wahab OI,Healy B,Dzik WH

    更新日期:2006-08-01 00:00:00

  • Effects of hydroxyethyl starch on blood coagulation, particularly factor VIII.

    abstract::The effects of hydroxyethyl starch (HES) on hemostasis were investigated extensively. In order to simulate acute blood loss due to surgery or trauma, one unit (450 ml) of blood was drawn from normal healthy men. This was followed by a 1-liter infusion over 60 minutes of either 6 percent HES, 5 percent albumin, or 0.9 ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1985.25485273815.x

    authors: Stump DC,Strauss RG,Henriksen RA,Petersen RE,Saunders R

    更新日期:1985-07-01 00:00:00

  • Platelets photochemically treated with amotosalen HCl and ultraviolet A light correct prolonged bleeding times in patients with thrombocytopenia.

    abstract:BACKGROUND:Photochemical treatment (PCT) with amotosalen HCl with ultraviolet A illumination inactivates pathogens and white blood cells in platelet (PLT) concentrates. STUDY DESIGN AND METHODS:In a Phase II crossover study, 32 patients with thrombocytopenia received one transfusion of PCT and/or one transfusion of un...

    journal_title:Transfusion

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/j.1537-2995.2006.00791.x

    authors: Slichter SJ,Raife TJ,Davis K,Rheinschmidt M,Buchholz DH,Corash L,Conlan MG

    更新日期:2006-05-01 00:00:00

  • The risk of transfusion-transmitted infections in sub-Saharan Africa.

    abstract:BACKGROUND:Blood transfusions carry the risk of transmitting infections. This risk has been studied in detail in high-income countries but not in sub-Saharan Africa. This study estimates the risks of acquiring human immunodeficiency virus (HIV), hepatitis B virus (HBV), or hepatitis C virus (HCV) from a single unit of ...

    journal_title:Transfusion

    pub_type: 杂志文章,评审

    doi:10.1111/j.1537-2995.2009.002402.x

    authors: Jayaraman S,Chalabi Z,Perel P,Guerriero C,Roberts I

    更新日期:2010-02-01 00:00:00

  • New HIV infections from blood transfusions averted in 28 countries supported by PEPFAR blood safety programs, 2004-2015.

    abstract:BACKGROUND:To quantify the impact of the US President's Emergency Plan for AIDS Relief (PEPFAR) on the risk of HIV transmission through infected blood donations in countries supported by PEPFAR blood safety programs. METHODS:Data reported to the World Health Organization Global Database on Blood Safety were analyzed f...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.16256

    authors: Mili FD,Teng Y,Shiraishi RW,Yu J,Bock N,Drammeh B,Watts DH,Benech I

    更新日期:2021-01-28 00:00:00

  • Major transfusions remain frequent despite the generalized use of tranexamic acid: an audit of 3322 patients undergoing cardiac surgery.

    abstract:BACKGROUND:Aprotinin has been reapproved for use in Europe and in Canada. We sought to determine if its reintroduction was still pertinent given the widespread administration of tranexamic acid, another antifibrinolytic shown to reduce bleeding and transfusions. STUDY DESIGN AND METHODS:After institutional review boar...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.13615

    authors: Stevens LM,Noiseux N,Prieto I,Hardy JF

    更新日期:2016-07-01 00:00:00

  • Prevalence of the newly described human circovirus, TTV, in United States blood donors.

    abstract:BACKGROUND:A novel nonenveloped single-stranded circular DNA virus (TTV) was recently identified. The prevalence of TTV in blood donors in the United States is, however, still unclear. STUDY DESIGN AND METHODS:Viral DNA was detected in US blood donors from five cities by using two sets of TTV primers: NG059/NG061/NG06...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2000.40020245.x

    authors: Handa A,Dickstein B,Young NS,Brown KE

    更新日期:2000-02-01 00:00:00