Direct identification of Yersinia enterocolitica in blood by polymerase chain reaction amplification.

Abstract:

:Primers based on the nucleotide sequence of the virF gene in the pYV plasmid and the chromosomal ail gene were used in polymerase chain reaction (PCR) amplifications to directly identify Yersinia enterocolitica in blood. Approximately 500 bacteria seeded into 100 microL of blood can be extracted and amplified by PCR to yield positive results. PCR analyses of seven Y. enterocolitica isolates previously implicated in blood contaminations showed that only one isolate harbored the plasmid-borne virF gene; however, all seven isolates were identified effectively by the PCR product amplified from the chromosomal gene. The PCR assay has the potential for use in the identification of Y. enterocolitica contamination in stored units of blood or in the rapid diagnosis of transfusion-related bacteremia caused by Y.

journal_name

Transfusion

journal_title

Transfusion

authors

Feng P,Keasler SP,Hill WE

doi

10.1046/j.1537-2995.1992.32993110759.x

subject

Has Abstract

pub_date

1992-11-01 00:00:00

pages

850-4

issue

9

eissn

0041-1132

issn

1537-2995

journal_volume

32

pub_type

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