A pattern of 5-1-1 and c100-3 only on hepatitis C virus (HCV) recombinant immunoblot assay does not reflect HCV infection in blood donors.

Abstract:

:Current criteria for a reactive (positive) interpretation on hepatitis C virus (HCV) recombinant immunoblot assay (RIBA) require > or = 1+ reactivity to at least two of the four HCV antigens present in the assay. Given that 5-1-1 is a subcomponent of c100-3, there is concern that donor samples reacting only with these two antigens (and not with c22-3 or c33c) could be incorrectly classified as positive on the basis of limited reactivity to only one HCV gene product. It is determined that 0.23 to 0.44 percent of HCV enzyme immunoassay-repeatably reactive donor sera demonstrate a pattern of 5-1-1 and c100-3 only on RIBA. Evaluation of six such donor sera using peptide enzyme immunoassays spanning the c100-3 antigen showed highly restricted reactivity to the 5-1-1 N-terminal region of c100-3, in contrast to broad 5-1-1 and c100-3 C-terminal peptide reactivity observed in the majority of donor sera with other positive RIBA patterns. HCV polymerase chain reaction and follow-up serologic evaluations of four of these donors indicated the absence of viremia or evolving seroconversion in all cases. It is concluded that, in the blood donor setting, a pattern of only 5-1-1 and c100-3 reactivity is typically not indicative of HCV infection. To avoid overinterpretation, it is recommended that RIBA grading criteria be revised to require reactivity to two or more HCV-encoded gene products.

journal_name

Transfusion

journal_title

Transfusion

authors

Busch MP,Tobler L,Quan S,Wilber JC,Johnson P,Polito A,Steane E,Zola A,Bahl C,Nelles M

doi

10.1046/j.1537-2995.1993.33193142316.x

subject

Has Abstract,Author List Incomplete

pub_date

1993-01-01 00:00:00

pages

84-8

issue

1

eissn

0041-1132

issn

1537-2995

journal_volume

33

pub_type

杂志文章
  • Human herpesvirus 8 genomes and seroprevalence in United States blood donors.

    abstract:BACKGROUND:Human herpesvirus 8 (HHV-8) is a gamma-herpesvirus that causes Kaposi's sarcoma. The prevalence of HHV-8 genomes in US blood donors has not been systemically studied. STUDY DESIGN AND METHODS:A sensitive and quantitative real-time polymerase chain reaction (PCR) assay was used to detect HHV-8 DNA from purif...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2009.02559.x

    authors: Qu L,Jenkins F,Triulzi DJ

    更新日期:2010-05-01 00:00:00

  • Influence of enteral feeding and anemia on tissue oxygen extraction after red blood cell transfusion in preterm infants.

    abstract:BACKGROUND:Understanding factors that impact tissue oxygen extraction may guide red blood cell (RBC) transfusion decision making in preterm infants. Our objective was to assess the influence of enteral feeding and anemia on cerebral and mesenteric oxygen saturation (Csat and Msat) and fractional tissue oxygen extractio...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15680

    authors: Goldstein GP,Rao A,Ling AY,Ding VY,Chang IJ,Chock VY

    更新日期:2020-03-01 00:00:00

  • Prevention of transfusion-associated graft-versus-host disease: selection of an adequate dose of gamma radiation.

    abstract::To determine the optimal dose of gamma radiation necessary to inhibit T-lymphocyte function and prevent transfusion-acquired graft-versus-host disease (TA-GVHD), a donor plateletpheresis component was initially divided into ten 20-mL samples. One sample was not irradiated, while the other nine samples were treated wit...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1993.33293158043.x

    authors: Rosen NR,Weidner JG,Boldt HD,Rosen DS

    更新日期:1993-02-01 00:00:00

  • Obtaining an accepted Investigational New Drug application to operate an umbilical cord blood bank.

    abstract:BACKGROUND:The residual blood left in the placenta, previously considered a biologic waste, contains sufficient hematopoietic stem and progenitor cells to consistently engraft at least a small recipient. Over the past several years, more than 500 HLA-matched, related and unrelated, allogeneic cord blood transplants hav...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1999.39499235666.x

    authors: Reems JA,Fujita D,Tyler T,Moldwin R,Smith SD

    更新日期:1999-04-01 00:00:00

  • Another potential source of error in Rh-hr typing.

    abstract::Three cases are presented in which the presence of sodium caprylate dependent albumin auto-agglutinins in the patients' sera resulted in erroneous Rh-hr phenotyping of their erythrocytes. In the initial testing, the albumin control was negative in each case. Since one of the cases was an Rh0(D) negative individual who...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1975.15576082226.x

    authors: Reid ME,Ellisor SS,Frank BA

    更新日期:1975-09-01 00:00:00

  • The influence of bleeding on trigger changes for platelet transfusion in patients with chemotherapy-induced thrombocytopenia.

    abstract:BACKGROUND:For patients with thrombocytopenia without bleeding risk factors, a platelet transfusion trigger of 10 × 10(9) /L is recommended. No studies have evaluated the clinicians' decision-making process leading to trigger changes. STUDY DESIGN AND METHODS:We report on the evaluation of trigger changes and the rela...

    journal_title:Transfusion

    pub_type: 临床试验,杂志文章

    doi:10.1111/j.1537-2995.2012.03727.x

    authors: Rioux-Massé B,Laroche V,Bowman RJ,Lindgren BR,Cohn CS,Pulkrabek SM,McCullough J

    更新日期:2013-02-01 00:00:00

  • The prevalence of anti-HCV among Chinese voluntary blood donors in Taiwan.

    abstract::In Taiwan, the prevalence of circulating anti-HCV is 2 percent among first-time voluntary Chinese blood donors, 10 percent among donors with elevated ALT levels (greater than 45 IU/L), and higher among older men. The carrier rate for HBsAg was 18.6 percent and the frequency of positive HBV marker(s) (HBsAg, anti-HBc, ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1990.30590296383.x

    authors: Lin-Chu M,Tsai SJ,Watanabe J,Nishioka K

    更新日期:1990-06-01 00:00:00

  • Blood supply safety in Afghanistan: a national assessment of high-volume facilities.

    abstract:BACKGROUND:Little information is available regarding blood supply safety in Afghanistan. The purpose of this study was to assess blood safety through serologic and observational measures in Afghanistan. STUDY DESIGN AND METHODS:This cross-sectional assessment included the 40 highest-volume facilities collecting and tr...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12005

    authors: Mansoor GF,Rahmani AM,Kakar MA,Hashimy P,Abrahimi P,Scott PT,Peel SA,Rentas FJ,Todd CS

    更新日期:2013-09-01 00:00:00

  • Role of glycoprotein Ibalpha in phagocytosis of platelets by macrophages.

    abstract:BACKGROUND:Platelet (PLT) storage at 0 to 4 degrees C suppresses bacterial multiplication, but induces clusters of glycoprotein (GP) Ibalpha that trigger their phagocytosis by macrophages and reduce their survival after transfusion. A method was sought that detects cold-induced changes in GPIbalpha involved in phagocyt...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2006.01034.x

    authors: Badlou BA,Spierenburg G,Ulrichts H,Deckmyn H,Smid WM,Akkerman JW

    更新日期:2006-12-01 00:00:00

  • Demonstration of gene dosage effects on antigens in the Duffy, Ss, and Rh systems using an enzyme-linked immunosorbent assay.

    abstract::Using a recently developed enzyme-linked immunosorbent assay, gene dosage effects were demonstrated for the Duffy, Ss, and Rh red blood cell antigen systems. We report a comparison by an antibody-binding assay of the relative amounts of Fya and Fyb antigens on FyaFya, FyaFyb, FybFyb, FyaFyx, and FyxFyx erthrocytes, an...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1982.22683068606.x

    authors: Caren LD,Bellavance R,Grumet FC

    更新日期:1982-11-01 00:00:00

  • Effect of screening for red cell antibodies, other than anti-D, to detect hemolytic disease of the fetus and newborn: a population study in the Netherlands.

    abstract:BACKGROUND:Hemolytic disease of the fetus and newborn (HDFN) is a severe disease, resulting from maternal red cell (RBC) alloantibodies directed against fetal RBCs. The effect of a first-trimester antibody screening program on the timely detection of HDFN caused by antibodies other than anti-D was evaluated. STUDY DES...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2007.01625.x

    authors: Koelewijn JM,Vrijkotte TG,van der Schoot CE,Bonsel GJ,de Haas M

    更新日期:2008-05-01 00:00:00

  • An acute hemolytic transfusion reaction caused by an anti-P1 that reacted at 37 degrees C.

    abstract:BACKGROUND:Hemolytic transfusion reactions (HTRs) due to anti-P1 have rarely been reported. There is only one report (from 1945) of an acute HTR due to anti-P1. CASE REPORT:A 74-year-old woman with anti-P1 was given blood that had been found to be compatible by the use of prewarmed serum and saline-suspended red cells...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1998.38498257376.x

    authors: Arndt PA,Garratty G,Marfoe RA,Zeger GD

    更新日期:1998-04-01 00:00:00

  • A prospective observational study of acute traumatic coagulopathy in traumatic bleeding from the battlefield.

    abstract:BACKGROUND:Acute trauma coagulopathy (ATC) after military trauma has not been comprehensively studied. ATC is defined as a prolonged prothrombin time ratio (PTr) or reduced clot amplitude (A5) in viscoelastic testing. Compared to civilian trauma, military trauma has more injuries from explosions and gunshot wounds (GSW...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15658

    authors: Woolley T,Gwyther R,Parmar K,Kirkman E,Watts S,Midwinter M,Lucca JD,Hunt BJ

    更新日期:2020-06-01 00:00:00

  • Collection and transfusion of blood and blood components in the United States, 1994.

    abstract:BACKGROUND:Collections and transfusions of blood in the United States in 1994 were measured and compared with those in 1992. STUDY DESIGN AND METHODS:Completed survey questionnaires were returned by all 147 regional blood centers, 1340 American Association of Blood Banks (AABB) member hospitals, and 523 non-AABB hospi...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1998.38798346630.x

    authors: Wallace EL,Churchill WH,Surgenor DM,Cho GS,McGurk S

    更新日期:1998-07-01 00:00:00

  • Platelet glycolytic flux increases stimulated by ultraviolet-induced stress is not the direct cause of platelet morphology and activation changes: possible implications for the role of glucose in platelet storage.

    abstract:BACKGROUND:Stress-enhanced platelet (PLT) storage lesions include increased glycolysis, discoid-to-sphere morphology change, and spontaneous PLT activation. It is not clear if reduction in glycolysis can alleviate storage lesion development. STUDY DESIGN AND METHODS:Apheresis PLT concentrates were exposed to 17.2 J/mL...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2005.00582.x

    authors: Li J,Goodrich L,Hansen E,Edrich R,Gampp D,Goodrich RP

    更新日期:2005-11-01 00:00:00

  • Can family or replacement blood donors become regular volunteer donors?

    abstract:BACKGROUND:In sub-Saharan Africa (SSA) confirmed viral marker prevalence between family donors (FDs) and first-time volunteer nonremunerated donors (VNRDs) is similar. In a blood service collecting 10 units/1000 inhabitants, a questionnaire examined FD donation conditions and willingness of becoming repeat VNRDs. STUD...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.12216

    authors: Asenso-Mensah K,Achina G,Appiah R,Owusu-Ofori S,Allain JP

    更新日期:2014-03-01 00:00:00

  • Hepatitis E virus antibody prevalence, RNA frequency, and genotype among blood donors in Cambodia (Southeast Asia).

    abstract:BACKGROUND:Recent studies conducted in developed countries described hepatitis E virus (HEV) as an emerging infectious threat to blood safety. However, data on HEV among blood donors from southeast Asia are lacking. STUDY DESIGN AND METHODS:Between July and August 2014, we assessed the presence of HEV immunoglobulin (...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.13731

    authors: Nouhin J,Prak S,Madec Y,Barennes H,Weissel R,Hok K,Pavio N,Rouet F

    更新日期:2016-10-01 00:00:00

  • Blood groups: the past 50 years.

    abstract::Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became available, our ability to discover and el...

    journal_title:Transfusion

    pub_type: 历史文章,杂志文章

    doi:10.1111/j.1537-2995.2009.02456.x

    authors: Daniels G,Reid ME

    更新日期:2010-02-01 00:00:00

  • Influence of climate on donor deferrals.

    abstract::The purpose of this study was to ascertain the cause for seasonal variations of donor deferral. Deferred donors and monthly temperatures from 1981 to 1984 were tallied and analyzed. Temperatures were categorized at 10-degree intervals from 15 degrees F to 75 degrees F. Donors failing the hemoglobin screening then unde...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1988.28689059031.x

    authors: Lau P,Hansen M,Sererat M

    更新日期:1988-11-01 00:00:00

  • Blood preservation. XXVIII. Galactose and maltose maintain red blood cell 2,3-DPG and ATP.

    abstract::Because there may be inadequate dextrose in the newly licensed CPD-adenine for five or six weeks storage of high hematocrit red blood cells, this laboratory has examined some alternate sugars for their ability to maintain red blood cell metabolism during storage. In the current study, dextrose and fructose were studie...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1980.20180125025.x

    authors: Dawson RB,Hershey RT,Myers CS,Zuck TF

    更新日期:1980-01-01 00:00:00

  • Preventing murine transfusion-related acute lung injury by expansion of CD4+ CD25+ FoxP3+ Tregs using IL-2/anti-IL-2 complexes.

    abstract:BACKGROUND:Transfusion-related acute lung injury (TRALI) is one of the most serious adverse events following transfusion, and there is no specific treatment in clinical practice. However, regulatory T cells (Tregs) have been suggested to play a potential role in the treatment of TRALI. This study investigated whether i...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15064

    authors: He R,Li L,Kong Y,Tian L,Tian X,Fang P,Bian M,Liu Z

    更新日期:2019-02-01 00:00:00

  • Hemolytic markers following the transfusion of uncrossmatched, cold-stored, low-titer, group O+ whole blood in civilian trauma patients.

    abstract:BACKGROUND:Low-titer group O whole blood (LTOWB) is increasingly being used in the civilian trauma setting, although there is a risk of hemolysis. This study evaluated the impact on hemolytic markers following the transfusion of 4 or more units of uncrossmatched LTOWB. METHODS:Civilian adult trauma patients who receiv...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15629

    authors: Harrold IM,Seheult JN,Alarcon LH,Corcos A,Sperry JL,Triulzi DJ,Yazer MH

    更新日期:2020-06-01 00:00:00

  • Harmful effects of transfusion of older stored red blood cells: iron and inflammation.

    abstract::Retrospective studies suggest that the transfusion of older, stored red blood cells (RBCs) may be associated with increases in mortality, serious infections, multiorgan failure, thrombosis, and hospital length of stay. Our research is based on the overarching hypothesis that the adverse effects associated with transfu...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2011.03096.x

    authors: Hod EA,Spitalnik SL

    更新日期:2011-04-01 00:00:00

  • Systematic review of rituximab for autoimmune diseases: a potential alternative to intravenous immune globulin.

    abstract:BACKGROUND:The anti-CD20 monoclonal antibody rituximab has immune-modulatory effects similar to intravenous immunoglobulin (IVIG). We performed a systematic review and meta-analysis to determine the efficacy and safety of rituximab in autoimmune diseases that are also treated with IVIG. STUDY DESIGN AND METHODS:The mo...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.14841

    authors: MacIsaac J,Siddiqui R,Jamula E,Li N,Baker S,Webert KE,Evanovitch D,Heddle NM,Arnold DM

    更新日期:2018-11-01 00:00:00

  • Transient hemolysis due to anti-D and anti-A1 produced by engrafted donor's lymphocytes after allogeneic unmanipulated haploidentical hematopoietic stem cell transplantation.

    abstract:BACKGROUND:Development of de novo alloantibodies against recipient's red blood cell (RBC) antigens by engrafted donor's lymphocytes is a known phenomenon in the setting of allogeneic hematopoietic stem cell transplantation (HSCT). This situation is usually clinically insignificant. We report a case of early clinically ...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.14232

    authors: Bailén R,Kwon M,Pérez-Corral AM,Pascual C,Buño I,Balsalobre P,Serrano D,Gayoso J,Díez-Martín JL,Anguita J

    更新日期:2017-10-01 00:00:00

  • Estimated risk of Chikungunya viremic blood donation during an epidemic on Reunion Island in the Indian Ocean, 2005 to 2007.

    abstract:BACKGROUND:Between 2005 and 2007, Chikungunya virus (CHIKV) caused a massive epidemic on Reunion Island with a major peak in the number of cases in February 2006. Blood donation was interrupted on the island in January 2006. STUDY DESIGN AND METHODS:Estimates of the mean risk of viremic blood donation on Reunion Islan...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2008.01646.x

    authors: Brouard C,Bernillon P,Quatresous I,Pillonel J,Assal A,De Valk H,Desenclos JC,workgroup \Quantitative Estimation of the Risk of Blood Donation Contamination by Infectious Agents\.

    更新日期:2008-07-01 00:00:00

  • Platelets photochemically treated with amotosalen HCl and ultraviolet A light correct prolonged bleeding times in patients with thrombocytopenia.

    abstract:BACKGROUND:Photochemical treatment (PCT) with amotosalen HCl with ultraviolet A illumination inactivates pathogens and white blood cells in platelet (PLT) concentrates. STUDY DESIGN AND METHODS:In a Phase II crossover study, 32 patients with thrombocytopenia received one transfusion of PCT and/or one transfusion of un...

    journal_title:Transfusion

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/j.1537-2995.2006.00791.x

    authors: Slichter SJ,Raife TJ,Davis K,Rheinschmidt M,Buchholz DH,Corash L,Conlan MG

    更新日期:2006-05-01 00:00:00

  • Use of dextran 75 as a macromolecular agent in centrifugal leukapheresis.

    abstract::Twenty-five leukaphereses were done by the Haemonetics system using citrated Dextran 75 in normal saline as anticoagulant, and were compared with a much larger series using hydroxyethyl starch (HES) with and without steroid premedication. The granulocyte and platelet yields were only minimally different between the tw...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1977.17277151921.x

    authors: Huestis DW

    更新日期:1977-03-01 00:00:00

  • Identification of new KLF1 and LU alleles during the resolution of Lutheran typing discrepancies.

    abstract:BACKGROUND:The Lu(b) antigen is expressed on red blood cells (RBCs) of the majority of individuals in all populations. Its absence in transfused patients may lead to alloantibody production and mild-to-moderate transfusion reactions, and in pregnancies to mild hemolytic disease of the fetus and newborn. This report des...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.13556

    authors: Garcia-Sanchez F,Pardi C,Kupatawintu P,Thornton N,Rodriguez MA,Lucea I,Sood C,Ochoa-Garay G

    更新日期:2016-06-01 00:00:00

  • Genetic diversity of KELnull and KELel: a nationwide Austrian survey.

    abstract:BACKGROUND:Besides ABO and RH, the KEL blood group system, including the two antithetical antigens KEL1 and KEL2, is the most important owing to the frequent appearance of anti-KEL alloantibodies and their considerable clinical significance. So far, only limited information was available on KEL variant alleles determin...

    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2007.01174.x

    authors: Körmöczi GF,Wagner T,Jungbauer C,Vadon M,Ahrens N,Moll W,Mühlbacher A,Ozgül-Gülce S,Kleinrath T,Kilga-Nogler S,Schönitzer D,Gassner C

    更新日期:2007-04-01 00:00:00