Effect of blood donation on the establishment of normal ranges of lymphocyte subsets.

Abstract:

BACKGROUND:Absolute counts of CD4+ T-lymphocytes are used in the management of patients with human immunodeficiency virus infection. Low absolute counts of CD3+CD4+ cells have also been observed in healthy people--a phenomenon called idiopathic CD4 lymphocytopenia. It is common practice for normal ranges for lymphocyte subsets to be derived from samples taken from blood donors. STUDY DESIGN AND METHODS:A sample of EDTA blood was taken through the donation line tubing, after donation from 565 blood donors in Sydney, Australia, who were selected from a range of age groups. An additional 12 donors provided a predonation sample as well as a postdonation sample. Hematologic assays were performed on two analyzers. Samples were stained for CD3, CD4, CD8, CD19, and CD56 and analyzed on a flow cytometer. RESULTS:Three donors were found to have absolute CD3+CD4+ counts < 300 cells per microL. The percentage of CD3+CD4+ cells was found to increase with age. Both the percentage and the absolute count of CD3+CD8+ cells decreased with age, which resulted in an increased CD4:CD8 ratio with age. Men had consistently higher absolute counts of CD3-CD56+ cells than women. The 12 additional donors all had greater percentages of CD3+CD4+ cells and lower absolute counts for CD3+, CD3+CD4+, CD3+CD8+, CD19+ and CD3-CD56+ cells after donation than they had before donation (p < 0.001). CONCLUSION:It is not satisfactory to base normal ranges for lymphocyte subsets on donor blood, from which the blood sample has been obtained after donation.

journal_name

Transfusion

journal_title

Transfusion

authors

Bryant JA,Wylie BR,Yuan FF,Ribeiro A,Thomson AR,Cooley MA,Fletcher A

doi

10.1046/j.1537-2995.1996.36696269517.x

subject

Has Abstract

pub_date

1996-06-01 00:00:00

pages

559-66

issue

6

eissn

0041-1132

issn

1537-2995

journal_volume

36

pub_type

杂志文章
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    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1980.20480260268.x

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    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1996.36111297091734.x

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    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1976.16577039304.x

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  • Transfusion and component characteristics are not associated with allergic transfusion reactions to apheresis platelets.

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    journal_title:Transfusion

    pub_type: 临床试验,杂志文章

    doi:10.1111/trf.12852

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    更新日期:2015-02-01 00:00:00

  • WBC-reduced platelet concentrates from pooled buffy coats in additive solution: an evaluation of in vitro and in vivo measures.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.2001.41070917.x

    authors: van der Meer PF,Pietersz RN,Tiekstra MJ,Huijgens PC,Dekker WJ,Reesink HW

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  • Predonation hematocrit measurement by whole blood electrical conductivity assay.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1985.25385219908.x

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  • Plasma ectonucleotidases prevent desensitization of purinergic receptors in stored platelets: importance for platelet activity during thrombus formation.

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    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2006.00837.x

    authors: Cauwenberghs S,Feijge MA,Hageman G,Hoylaerts M,Akkerman JW,Curvers J,Heemskerk JW

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  • Motivation and social capital among prospective blood donors in three large blood centers in Brazil.

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    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2012.03887.x

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    authors: Reiher VSA,Hönger G,Infanti L,Passweg JR,Hösli I,Frey BM,Gassner C,Meyer S,Buser AS,Holbro A,Schaub S

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  • Long-term production of pre-existing alloantibodies to E and c after allogenic BMT in a patient with aplastic anemia resulting in delayed hemolytic anemia.

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    pub_type: 杂志文章

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    authors: Tran MH,Lin DM,Wilcox T,Schiro D,Cannesson M,Milliken J

    更新日期:2014-10-01 00:00:00

  • Red blood cell alloimmunization is associated with lower expression of FcγR1 on monocyte subsets in patients with sickle cell disease.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15463

    authors: Balbuena-Merle R,Curtis SA,Devine L,Gibb DR,Karafin MS,Luckey CJ,Tormey CA,Siddon AJ,Roberts JD,Hendrickson JE

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  • Variation in concentration of prion protein in the peripheral blood of patients with variant and sporadic Creutzfeldt-Jakob disease detected by dissociation enhanced lanthanide fluoroimmunoassay and flow cytometry.

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    pub_type: 临床试验,杂志文章

    doi:10.1111/j.0041-1132.2005.04342.x

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.16000

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  • An analysis on the fate of a selection of blood products derived from cytomegalovirus-seronegative donors at three tertiary referral hospitals in Australia.

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    journal_title:Transfusion

    pub_type: 临床试验,杂志文章,多中心研究

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  • Evaluation and improvement of blood donor educational materials: results from a multicenter randomized controlled trial.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.15866

    authors: Wehrli G,Rossmann SN,Waxman DA,Katz LM

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  • Relationship of the time of storage and transfusion reactions to platelet concentrates from buffy coats.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1997.37597293886.x

    authors: Riccardi D,Raspollini E,Rebulla P,Pappalettera M,Marangoni F,Greppi N,Sirchia G

    更新日期:1997-05-01 00:00:00

  • Red blood cell hemolysis during blood bank storage: using national quality management data to answer basic scientific questions.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2009.02275.x

    authors: Hess JR,Sparrow RL,van der Meer PF,Acker JP,Cardigan RA,Devine DV

    更新日期:2009-12-01 00:00:00

  • Blood preservation. XXVIII. Galactose and maltose maintain red blood cell 2,3-DPG and ATP.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1980.20180125025.x

    authors: Dawson RB,Hershey RT,Myers CS,Zuck TF

    更新日期:1980-01-01 00:00:00

  • Evidence of acute primary occult hepatitis B virus infection in an Italian repeat blood donor.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2008.02041.x

    authors: Manzini P,Abate ML,Valpreda C,Milanesi P,Curti F,Rizzetto M,Smedile A

    更新日期:2009-04-01 00:00:00

  • A tale of two ports: an in vitro comparison of flow characteristics for therapeutic plasma exchange.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1111/trf.14494

    authors: De Simone N,Sarode R

    更新日期:2018-02-01 00:00:00

  • Antibodies to vaccine antigens in pooled polyclonal human IgG products.

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    journal_title:Transfusion

    pub_type: 杂志文章,评审

    doi:10.1111/trf.15017

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    更新日期:2018-12-01 00:00:00

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    pub_type: 杂志文章

    doi:10.1111/trf.12141

    authors: Kurnaz F,Sivgin S,Pala C,Yildirim R,Baldane S,Kaynar L,Solmaz M,Ozturk A,Eser B,Cetin M,Unal A

    更新日期:2013-11-01 00:00:00

  • High prevalence of infectious events in thrombotic thrombocytopenic purpura and genetic relationship with toll-like receptor 9 polymorphisms: experience of the French Thrombotic Microangiopathies Reference Center.

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    doi:10.1111/trf.12263

    authors: Morgand M,Buffet M,Busson M,Loiseau P,Malot S,Amokrane K,Fortier C,London J,Bonmarchand G,Wynckel A,Provôt F,Poullin P,Vanhille P,Presne C,Bordessoule D,Girault S,Delmas Y,Hamidou M,Mousson C,Vigneau C,Lautrette A

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    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2008.01772.x

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    更新日期:2008-07-01 00:00:00

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    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2007.01074.x

    authors: Scott EA,Puca KE,Pietz BC,Duchateau BK,Friedman KD

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    pub_type: 杂志文章

    doi:10.1111/j.1537-2995.2010.02720.x

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  • In vivo red cell destruction by anti-Lu6.

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    journal_title:Transfusion

    pub_type: 杂志文章

    doi:10.1046/j.1537-2995.1990.30390194349.x

    authors: Issitt PD,Valinsky JE,Marsh WL,DiNapoli J,Gutgsell NS

    更新日期:1990-03-01 00:00:00