Abstract:
:An isolated vascularized bone marrow transplant (iVBMT) model was previously developed in the rat to specifically study the role of bone marrow and its environment in a composite tissue allotransplant. An extraperitoneal model was successfully created to avoid laparotomy and cross-clamping of the great vessels. The extraperitoneal iVBMT model consisted of a left donor femur that was harvested with its nutrient vessels, anastomosed to the right femoral vessels in a syngeneic host, and then placed subcutaneously in the abdominal wall. At explant, the graft vessels were grossly patent, and histology of the graft bones showed a viable marrow compartment. Polymerase chain reaction demonstrated peripheral chimerism in the recipients. This model is technically simple with minimal morbidity in the recipient animals. By using the iVBMT, future studies across semiallogeneic and allogeneic barriers will help define the role of the bone marrow compartment in composite tissue allotransplants to potentially induce immune tolerance.
journal_name
Transplantationjournal_title
Transplantationauthors
Tai CY,France MA,Strande LF,Eydelman R,Sheng X,Matthews MS,Dalsey R,Hewitt CWdoi
10.1097/01.TP.0000061490.85273.29subject
Has Abstractpub_date
2003-05-15 00:00:00pages
1591-3issue
9eissn
0041-1337issn
1534-6080journal_volume
75pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract:BACKGROUND:Circulation of leukocytes via blood, tissue and lymph is integral to adaptive immunity. Afferent lymphatics form CCL21 gradients to guide dendritic cells and T cells to lymphatics and then to draining lymph nodes (dLN). Vascular endothelial growth factor C and vascular endothelial growth factor receptor 3 (V...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000000561
更新日期:2015-04-01 00:00:00
abstract::Recent reports document the efficacy of transjugular intrahepatic portocaval shunts (TIPS) for the prevention of portal hypertensive bleeding and have advocated its use as a bridge to liver transplantation. There are no reports, however, analyzing liver transplant results for patients with indwelling TIPS. We reviewed...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1994-08-15 00:00:00
abstract:BACKGROUND:Allogeneic bone marrow transplantation (BMT) and donor lymphocyte infusion (DLI) provide valuable treatments for a range of diseases. However, the therapeutic utility of BMT and DLI is reduced by the high incidence of graft-versus-host disease (GvHD) mediated by activated donor T lymphocytes directed against...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000229435.58898.c5
更新日期:2006-08-27 00:00:00
abstract:BACKGROUND:Infections are a major cause of morbidity and mortality in kidney allograft recipients. In this post hoc analysis of a randomized clinical trial which tested the effect of denosumab on bone mineral density, we assessed the impact of this drug on the incidence and severity of infections in the first year afte...
journal_title:Transplantation
pub_type: 杂志文章,随机对照试验
doi:10.1097/TP.0000000000001547
更新日期:2017-09-01 00:00:00
abstract:BACKGROUND:Little is known about innate lymphoid cell (ILC) populations in the human gut, and the turnover of these cells and their subsets after transplantation has not been described. METHODS:Intestinal samples were taken from 4 isolated intestine and 3 multivisceral transplant recipients at the time of any operativ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000001593
更新日期:2017-10-01 00:00:00
abstract::Pulmonary endothelium is considered the compartment most susceptible to preservation damage. This investigation was designed to analyze the efficacy of an original, University of Parma low-potassium-albumin solution (SPAL UP) on cultured human pulmonary artery endothelial cells (HPAEC) and to compare its effects with ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199611150-00036
更新日期:1996-11-15 00:00:00
abstract::Antihuman lymphocyte rabbit (or horse) gamma globulins used in recipients of organ transplantation are prepared against thymocytes or immortalized cell lines, the only two sources so far allowing enough antigen preparation. These cells lack, however, the surface determinants characteristic of alloreactive blasts invol...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198908000-00014
更新日期:1989-08-01 00:00:00
abstract::Seven murine anti-H-2 and three nonimmune mouse sera were tested for cytotoxicity toward B and T lymphocytes from a panel of human donors. One group of sera, including two anti-H-2.33 sera, exhibited cytotoxicity directed exclusively toward human B but not T cells from all donors. Absorption studies on human lymphobla...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197908000-00007
更新日期:1979-08-01 00:00:00
abstract:BACKGROUND:The cascade of immunological effects brought about by donor bone marrow cell (DBMC) infusions in human organ transplantation, especially in the context of continuous pharmacologic immunosuppression, is not fully understood. Yet, in inbred rodents and even primates, administration of specific bone marrow cell...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199804150-00015
更新日期:1998-04-15 00:00:00
abstract:BACKGROUND:Deciphering the mechanisms of tolerance and chronic rejection (CR) remains a major goal in transplantation. Data in rodents suggest that Toll-like receptors (TLR), regulators of innate immune responses, play a role in determining graft outcome. However, few studies have focused on TLR expression in human kid...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31817b8dc5
更新日期:2008-07-15 00:00:00
abstract:BACKGROUND:Humanized Fc receptor (FcR)-nonbinding antibodies against CD3 are promising immunosuppressive agents that may overcome both the neutralizing response to and the cytokine release syndrome seen with conventional monoclonal antibodies against CD3. In addition, evidence from several murine models suggests that t...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200104150-00020
更新日期:2001-04-15 00:00:00
abstract::Spleen cells from B10.A mice transfused with B10.D2 blood suppress the immune responses of normal B10.A to B10.D2 in coculture as early as 2 days posttransfusion. In addition, the ability of B10.A mice to respond in cell-mediated lymphocytotoxicity (CML) is significantly impaired as early as 2 days after B10.D2 transf...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198805000-00018
更新日期:1988-05-01 00:00:00
abstract::Allotransplantation into immunosuppressed individuals results in long-term survival of grafts. However, the grafts are damaged, probably at many stages before, during and after implantation. The hypothesis to be presented is that release of antigens and autoantigens from the chronically damaged graft results in breaki...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/01.tp.0000153294.38939.5f
更新日期:2005-02-15 00:00:00
abstract:BACKGROUND:We have previously demonstrated that in a concordant hamster-to-nude rat cardiac transplant model, T-independent specific B-lymphocyte and natural killer (NK)-cell tolerance could be induced, leading to long-term xenograft (Xg) survival. Here, we investigated whether the same could be achieved in a clinicall...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199907150-00024
更新日期:1999-07-15 00:00:00
abstract::Hemorrhagic cystitis is a well known complication of allogeneic bone marrow transplantation (BMT) and is normally attributed to the use of high-dose cyclophosphamide in the preparative regimen. Hemorrhagic cystitis occurring late after BMT is unlikely to be due to the effects of this conditioning, and probably has an ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198701000-00024
更新日期:1987-01-01 00:00:00
abstract::We developed a multiplex, quantitative, real-time, polymerase chain reaction assay for cytomegalovirus (CMV) and used it to measure the CMV viral load in weekly blood specimens from 43 lung transplant recipients. The median viral load in blood samples immediately preceding bronchoscopy was 1150 copies/microg human DNA...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200108270-00030
更新日期:2001-08-27 00:00:00
abstract:OBJECTIVES:Recent studies demonstrated that prolactin (PRL) has beneficial effects on beta cells for islet transplantation. We examined the effect of human recombinant PRL (rhPRL) supplementation to the culture media to determine its potential use in the context of clinical islet transplantation. MATERIALS AND METHODS...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181d98af1
更新日期:2010-06-15 00:00:00
abstract:BACKGROUND:Our aim was to evaluate the effect of FTY720 in discordant islet xenotransplantation. METHODS:Fetal porcine islet-like cell clusters (ICCs) were transplanted into normoglycemic rats that were either left untreated or treated with FTY720 only, with FTY720 plus cyclosporine A (CsA) or with CsA only. Twelve or...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.TP.0000061770.39569.7F
更新日期:2003-04-27 00:00:00
abstract::We retrospectively reviewed the clinical data of all renal transplant patients treated with cyclosporine as their main chronic immunosuppressive agent between 12/83 and 11/85 to identify cytomegalovirus-negative patients at our institutions who received cytomegalovirus (CMV)-positive kidneys. Using a latex agglutinati...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198702000-00005
更新日期:1987-02-01 00:00:00
abstract::Osteopetrotic microphthalmic mice (mi/mi) were treated by injections of suspensions of myeloid tissue, newborns i.p., and weanlings i.v. Donated syngeneic material effected permanent cure of oteopetrosis provided that the dose was large enough (10(8) cells of bone marrow). H-2-compatible allogeneic bone marrow was ini...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197910000-00004
更新日期:1979-10-01 00:00:00
abstract::We studied major histocompatibility complex (MHC) and cytokine mRNA induction after renal injury in the absence of interferon-gamma (IFN-gamma) using IFN-gamma gene knockout (GKO) mice. The left renal pedicle of normal (wild-type) and GKO BALB/c mice was clamped for 60 minutes; cytokine and MHC mRNA expression were mo...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199560120-00031
更新日期:1995-12-27 00:00:00
abstract::We report two cases of Kaposi's sarcoma (KS) after orthotopic liver transplantation for cirrhosis of the liver related to hepatitis C virus. Both cases were Saudi-born Arabs who were negative for human immunodeficiency virus; one patient was receiving FK506 plus prednisolone, and the other patient was receiving FK506....
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199705270-00025
更新日期:1997-05-27 00:00:00
abstract:BACKGROUND:Both laboratory and clinical studies have shown that dietary lipids may affect immunologic responses. This study was conducted to compare different classes of long-chain unsaturated fatty acids for their effect on allograft survival in animals receiving a donor-specific transfusion and a short course of low-...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199805270-00004
更新日期:1998-05-27 00:00:00
abstract:BACKGROUND:The pathogenesis of acute pulmonary xenograft injury has not yet been determined. The present study evaluates the role of complement in mediating pulmonary xenograft dysfunction by using cobra venom factor (CVF) to deplete recipient complement and transgenic swine, which express human regulators of complemen...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199804270-00013
更新日期:1998-04-27 00:00:00
abstract:BACKGROUND:Intestinal allograft mucosa undergoes repopulation with host immunocytes. However, critical changes within key immunocyte subsets are not known. METHODS:To explain acute cellular rejection after intestine transplantation (ITx) on the basis of altered mucosal immunocytes, rejecting and rejection-free ITx all...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3182708d9d
更新日期:2012-12-27 00:00:00
abstract::When normal mice are exposed for short periods to ultraviolet light (UV), they support the progressive growth of transplanted syngeneic UV-induced tumors. Normal nonirradiated mice almost always reject these tumor implants. The UV-mediated suppression of the antitumor response can be adoptively transferred to normal s...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197708000-00005
更新日期:1977-08-01 00:00:00
abstract:BACKGROUND:The National Marrow Donor Program (NMDP) has approximately 5 million registered potential donors and continues to add more than 30,000 new recruits to the registry monthly. However, more than 30% of potential donors are not available or decline to donate at key decision points in the donation process. Althou...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000116394.88804.a7
更新日期:2004-03-27 00:00:00
abstract:BACKGROUND:Proteinuria is associated with endothelial dysfunction (ED) and increased mortality. We investigated whether urinary protein excretion (UPE) is correlated with markers of ED and whether these markers affect the association of proteinuria with mortality in renal transplant recipients (RTR). METHODS:Six hundr...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e3181903d25
更新日期:2008-12-27 00:00:00
abstract::The mode of action of glucocorticosteroids as immunosuppressive and antiinflammatory agents is not fully understood. Glucocorticosteroids block synthesis of interleukin 1 by interfering with the transcription of the IL-1 beta gene. Glucocorticosteroids may also induce rapid degradation of IL-1 mRNA. In the presence of...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199001000-00040
更新日期:1990-01-01 00:00:00
abstract::Balb/c (H-2d) mice were transfused weekly with 0.1 ml of whole blood from C3H (H-2k) mice. One week after 3 blood transfusions (BT), the mice were bled and the sera collected and pooled. The 3BT serum was absorbed twice with C3H lymphocytes and IgG isolated by ion-exchange chromatography. Balb/c anti-C3H, Balb/c anti-...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198612000-00011
更新日期:1986-12-01 00:00:00