An extraperitoneal isolated vascularized bone marrow transplant model in the rat.


:An isolated vascularized bone marrow transplant (iVBMT) model was previously developed in the rat to specifically study the role of bone marrow and its environment in a composite tissue allotransplant. An extraperitoneal model was successfully created to avoid laparotomy and cross-clamping of the great vessels. The extraperitoneal iVBMT model consisted of a left donor femur that was harvested with its nutrient vessels, anastomosed to the right femoral vessels in a syngeneic host, and then placed subcutaneously in the abdominal wall. At explant, the graft vessels were grossly patent, and histology of the graft bones showed a viable marrow compartment. Polymerase chain reaction demonstrated peripheral chimerism in the recipients. This model is technically simple with minimal morbidity in the recipient animals. By using the iVBMT, future studies across semiallogeneic and allogeneic barriers will help define the role of the bone marrow compartment in composite tissue allotransplants to potentially induce immune tolerance.






Tai CY,France MA,Strande LF,Eydelman R,Sheng X,Matthews MS,Dalsey R,Hewitt CW




Has Abstract


2003-05-15 00:00:00












  • Primary nonfunction of fatty livers produced by alcohol is associated with a new, antioxidant-insensitive free radical species.

    abstract::The formation of free radicals after orthotopic liver transplantation in the rat correlates with graft failure. Fatty livers from alcoholics transplant poorly, so these studies were designed to examine the effect of alcohol on free radical formation in a rearterialized rat liver transplantation model. Treatment of rat...


    pub_type: 杂志文章


    authors: Gao W,Connor HD,Lemasters JJ,Mason RP,Thurman RG

    更新日期:1995-03-15 00:00:00

  • Induction of carbon monoxide in donor animals prior to organ procurement reduces graft immunogenicity and inhibits chronic allograft dysfunction.

    abstract:BACKGROUND:Nonspecific inflammatory damages occurring prior to organ transplantation reduce long-term graft survival. Here, we tested the beneficial effects of carbon monoxide (CO) induction by methylene chloride (MC). METHODS:Fischer-344 (F-344 Rat) or Dark Agouti (DA Rat) donor animals were either treated with MC fo...


    pub_type: 杂志文章


    authors: Martins PN,Reutzel-Selke A,Jurisch A,Denecke C,Attrot K,Pascher A,Kotsch K,Pratschke J,Neuhaus P,Volk HD,Tullius SG

    更新日期:2006-10-15 00:00:00

  • Effect of preprocurement ventilation on lungs donated after cardiac death in a canine lung transplantation model.

    abstract:BACKGROUND:One method of countering chronic lung donor shortages is the practice of donation after cardiac death (DCD). However, this technique inevitably leads to pulmonary dysfunction related to warm ischemia. One promising method of alleviating this problem is ventilation. However, it can rarely be initiated from th...


    pub_type: 杂志文章


    authors: Sakamoto J,Chen F,Yamada T,Nakajima D,Ohsumi A,Kikuchi R,Zhao X,Fujinaga T,Shoji T,Sakai H,Bando T,Date H

    更新日期:2011-10-27 00:00:00

  • Apoptosis and the expression of Fas and Fas ligand (FasL) antigen in rejection and reinfection in liver allograft specimens.

    abstract:BACKGROUND AND METHODS:To investigate the frequency of apoptosis and the expression of Fas/Fas ligand (FasL) in liver allografts, we examined 97 biopsy specimens from 62 patients after orthotopic liver transplantation. The results of the biopsies were as follows: acute allograft rejection (n=32); hepatitis C virus (HCV...


    pub_type: 杂志文章


    authors: Tannapfel A,Kohlhaw K,Ebelt J,Hauss J,Liebert U,Berr F,Wittekind C

    更新日期:1999-04-15 00:00:00

  • Chronic cyclosporine-induced nephropathy in the rat. A medullary ray and inner stripe injury.

    abstract::Cyclosporine CsA nephrotoxicity was examined in male Sprague-Dawley rats with or without prior uninephrectomy, injected daily with 12.5 mg/kg CsA, and fed a salt-depleted or normal diet for 3-10 weeks. Control rats received the CsA vehicle. CsA induced a fall in creatinine clearance in salt-depleted rats, from 1.3 +/-...


    pub_type: 杂志文章


    authors: Rosen S,Greenfeld Z,Brezis M

    更新日期:1990-02-01 00:00:00

  • A schema for histologic grading of small intestine allograft acute rejection.

    abstract:BACKGROUND:Histologic evaluation of small bowel allograft biopsies is important for the diagnosis of acute rejection. However, a standard histologic schema to grade the severity of intestinal acute rejection is not currently available. The primary goal of this study was to develop a histologic grading system for the di...


    pub_type: 杂志文章


    authors: Wu T,Abu-Elmagd K,Bond G,Nalesnik MA,Randhawa P,Demetris AJ

    更新日期:2003-04-27 00:00:00

  • Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody.

    abstract::Monoclonal antibodies to CD3 have been shown to activate T cells in vivo and in vitro but have also been shown to render T cells anergic in vitro. In this study G4.18, a mouse IgG3 mAb, was produced that appeared to recognize CD3 by its binding to all peripheral T cells, including a population not recognized by mAb to...


    pub_type: 杂志文章


    authors: Nicolls MR,Aversa GG,Pearce NW,Spinelli A,Berger MF,Gurley KE,Hall BM

    更新日期:1993-03-01 00:00:00

  • Trends in Lung Transplantation Practices Across the United States During the COVID-19 Pandemic.

    abstract:BACKGROUND:The purpose of this study is to examine the effects of the coronavirus disease 2019 (COVID-19) pandemic on adult lung transplants and report practice changes in the United States. METHODS:A retrospective analysis of a public dataset from the United Network for Organ Sharing was performed regarding adult lun...


    pub_type: 杂志文章


    authors: Chan EG,Chan PG,Harano T,Ryan JP,Morrell MR,Sanchez PG

    更新日期:2021-01-01 00:00:00

  • Antibodies to hepatitis C virus envelope proteins correlate with hepatitis C viraemia after liver transplantation.

    abstract:BACKGROUND:Liver transplant recipients for hepatitis C virus (HCV)-related cirrhosis usually remain anti-HCV-seropositive after transplantation. The aim of this study was to characterize, longitudinally, the profile of HCV-specific antibodies and cryoglobulins in liver transplant recipients with recurrent HCV infection...


    pub_type: 杂志文章


    authors: Gane EJ,Maertens G,Ducatteeuw A,Qian KP,Lau JY,Jones H,Davies E,Naoumov NV,Williams R

    更新日期:1999-01-15 00:00:00

  • A possible explanation for anemia in patients treated with mycophenolic acid.

    abstract:BACKGROUND:Clinical studies suggest that the immunosuppressant mycophenolate mofetil is associated with anemia. However, the mechanism for this is not known. Here, we studied the effect of mycophenolic acid (MPA), the active metabolite of mycophenolate mofetil, on erythropoiesis in vitro. METHODS:Both UT-7 cells and p...


    pub_type: 杂志文章


    authors: Pile T,Kieswich J,Harwood S,Yaqoob MM

    更新日期:2011-12-27 00:00:00

  • Apoptosis versus necrosis during cold storage and rewarming of human renal proximal tubular cells.

    abstract:BACKGROUND:A recent clinical study demonstrated that in renal allografts preserved in the cold apoptosis occurred soon after reperfusion. The mode of cell death during cold storage is generally considered necrotic. Whether apoptosis occurs as a part of cold storage is uncertain. The objective was to determine in human ...


    pub_type: 杂志文章


    authors: Salahudeen AK,Joshi M,Jenkins JK

    更新日期:2001-09-15 00:00:00

  • Prevalence and risk factors of noncontrolled and resistant arterial hypertension in renal transplant recipients.

    abstract:BACKGROUND:Arterial hypertension (HT) is common in renal transplant recipients (RTRs). Control of HT is not optimal in this high-risk population despite recommendations for target blood pressure levels under 130/80 mm Hg. METHODS:We performed a cross-sectional analysis of the prevalence of uncontrolled HT, and using a...


    pub_type: 杂志文章


    authors: Vetromile F,Pernin V,Szwarc I,Garrigue V,Delmas S,Mourad G,Fesler P

    更新日期:2015-05-01 00:00:00

  • N-terminal fragments of the proatrial natriuretic peptide in plasma and urine of kidney graft recipients.

    abstract:BACKGROUND:Successful kidney transplantation normalizes elevated proatrial natriuretic peptide (proANP) plasma concentrations of renal failure patients in the early posttransplant period. We evaluated plasma and urinary proANP fragments in the late posttransplant period. METHODS:Immunoreactive proANP(1-30) and proANP(...


    pub_type: 杂志文章


    authors: Franz M,Woloszczuk W,Hörl WH

    更新日期:2001-07-15 00:00:00

  • Notch Signaling and Alloreactivity.

    abstract::Solid organ and allogeneic hematopoietic cell transplantation have become standard therapeutic interventions that save patient lives and improve quality of life. Our enhanced understanding of transplantation immunobiology has refined clinical management and improved outcomes. However, organ rejection and graft-versus-...


    pub_type: 杂志文章,评审


    authors: Radojcic V,Maillard I

    更新日期:2016-12-01 00:00:00

  • Ischemic preconditioning: a defense mechanism against the reactive oxygen species generated after hepatic ischemia reperfusion.

    abstract:BACKGROUND:Preconditioning protects against both liver and lung damage after hepatic ischemia-reperfusion (I/R). Xanthine and xanthine oxidase (XOD) may contribute to the development of hepatic I/R. OBJECTIVE:To evaluate whether preconditioning could modulate the injurious effects of xanthine/XOD on the liver and lung...


    pub_type: 杂志文章


    authors: Peralta C,Bulbena O,Xaus C,Prats N,Cutrin JC,Poli G,Gelpi E,Roselló-Catafau J

    更新日期:2002-04-27 00:00:00

  • Serum tumor markers after renal transplantation.

    abstract::The overall incidence of malignancy in renal transplant recipients is 100-fold higher compared with age matched controls. Routine clinical evaluation therefore often includes the determination of serum tumor markers AFP, CA19-9, CEA, CA125, CA15-3, PSA, and calcitonin. We evaluated the specificity and the sensitivity ...


    pub_type: 杂志文章


    authors: Oberbauer R,Banyai S,Schmidt A,Kornek G,Scheithauer W,Mayer G

    更新日期:1996-11-27 00:00:00

  • Qualitative and quantitative aspects of anti-H-2Ld sera.

    abstract::Two anti-H-2Ld sera were analyzed, BALB/c-H-2dm2 anti-BALB/cBy and (C3H X BALB/c-H-2dm2)F1 anti-BALB/cHe, the latter also containing anti-Qa antibodies. Their reaction patterns were compared with an anti-Qa serum (C3H X BALB/cBy)F1 anti-BALB/cHe. Four H-2 specificities could be detected by the anti-H-2Ld sera, two alr...


    pub_type: 杂志文章


    authors: Oudshoorn-Snoek M,Iványi D,Démant P

    更新日期:1981-08-01 00:00:00

  • Solid Organ Transplantation in Patients With Preexisting Malignancies in Remission: A Propensity Score Matched Cohort Study.

    abstract:BACKGROUND:Solid-organ transplant recipients with pretransplant malignancies (PTM) have worse overall survival (OS) compared to recipients without history of malignancy. However, it is unknown whether the increased risk of mortality is due to recurrent cancer-related deaths. METHODS:All solid-organ transplant recipien...


    pub_type: 杂志文章


    authors: Acuna SA,Sutradhar R,Kim SJ,Baxter NN

    更新日期:2018-07-01 00:00:00

  • Progression of renal dysfunction in cardiac transplantation after the introduction of everolimus in the immunosuppressive regime.

    abstract::The aim of this study was to analyze, in heart transplant patients, if renal function improvement after cyclosporine replacement by everolimus persists at the middle term and its predictors. We studied prospectively 56 patients in whom conversion was consecutively made. Forty-five patients completed the follow-up peri...


    pub_type: 杂志文章


    authors: Moro López JA,Almenar L,Martínez-Dolz L,Sánchez-Lázaro I,Agüero J,Buendía F,Ortiz V,Salvador A

    更新日期:2009-02-27 00:00:00

  • Correction of hyperoxaluria by liver repopulation with hepatocytes in a mouse model of primary hyperoxaluria type-1.

    abstract:BACKGROUND:Primary hyperoxaluria type-1 (PH1) is an autosomal recessive disease characterized by excessive oxalate production by hepatocytes caused by the deficiency of peroxisomal alanine-glyoxylate aminotransferase (AGT) activity. Persistent hyperoxaluria causes nephrocalcinosis and urolithiasis, leading to renal fai...


    pub_type: 杂志文章


    authors: Jiang J,Salido EC,Guha C,Wang X,Moitra R,Liu L,Roy-Chowdhury J,Roy-Chowdhury N

    更新日期:2008-05-15 00:00:00

  • How deceased donor transplantation is impacting a decline in commercial transplantation-the Tamil Nadu experience.

    abstract::India with a population of 1.2 billion has a renal transplantation rate of 3.25 per million population. The major cause of chronic kidney disease is hypertension and diabetes. The crude and age-adjusted incidence rates of end-stage renal disease are estimated to be 151 and 232 per million population, respectively, in ...


    pub_type: 杂志文章


    authors: Abraham G,Reddy YN,Amalorpavanathan J,Daniel D,Roy-Chaudhury P,Shroff S,Reddy Y

    更新日期:2012-04-27 00:00:00

  • Spontaneous rupture of the liver upon revascularization during transplantation.

    abstract::Spontaneous rupture of the liver has been described in association with many benign and malignant conditions. We report, to our knowledge, the first case of spontaneous rupture of the liver upon revascularization, requiring total hepatectomy and portocaval shunt, followed by successful retransplantation. Routine patho...


    pub_type: 杂志文章,评审


    authors: Mistry BM,Solomon H,Garvin PJ,Durham RM,Turnage S,Bacon BR,Galvin N,Varma CR

    更新日期:2000-05-27 00:00:00

  • CMV-specific Cell-mediated Immunity at 3-month Prophylaxis Withdrawal Discriminates D+/R+ Kidney Transplants at Risk of Late-onset CMV Infection Regardless the Type of Induction Therapy.

    abstract:BACKGROUND:Whether cytomegalovirus (CMV)-specific cell-mediated immunity (CMI) at prophylaxis cessation predicts D+/R+ kidney transplants at risk of late-onset CMV infection after receiving distinct induction therapies is still not well characterized. METHODS:We prospectively assessed CMV-specific CMI predicting late-...


    pub_type: 杂志文章


    authors: Jarque M,Melilli E,Crespo E,Manonelles A,Montero N,Torras J,Cruzado JM,Luque S,Gil-Vernet S,Grinyó JM,Bestard O

    更新日期:2018-11-01 00:00:00

  • HLA antibody screening: comparison of a solid phase enzyme-linked immunoassay with antiglobulin-augmented lymphocytotoxicity.

    abstract:BACKGROUND:IgG antibodies to HLA class I antigens can cause hyperacute rejection of renal allografts. Screening of sera from such transplant candidates is laborious, time-consuming, and expensive when performed by sensitive antihuman globulin-augmented lymphocytotoxicity (AHG-CDC). METHODS:Because 60-70% of our transp...


    pub_type: 临床试验,杂志文章


    authors: Moore SB,Ploeger NA,DeGoey SR

    更新日期:1997-12-15 00:00:00

  • Serum adiponectin levels and ambulatory blood pressure monitoring in pediatric renal transplant recipients.

    abstract::BACKGROUND.: Reduced levels of serum adiponectin, an adipokine, are associated with cardiovascular and obesity-related diseases; however, relations between adiponectin and hypertension are unclear. METHODS.: This cross-sectional study examined adiponectin and ambulatory blood pressure monitoring (ABPM) in 33 pediatric...


    pub_type: 杂志文章


    authors: Sethna CB,Leonard MB,Gallagher PR,Meyers KE

    更新日期:2009-10-27 00:00:00

  • Renal transplant from very old donors: how far can we go?

    abstract:BACKGROUND:The organ shortage has led many transplant centers to accept kidneys from old, suboptimal deceased donors, and make increasing use of old-for-old allocation systems. We report the experience of an Italian transplant center in the utilization of "ultra-old" (>75 years old) donors. METHODS:Sixty grafts from d...


    pub_type: 杂志文章


    authors: Collini A,Kalmar P,Dhamo A,Ruggieri G,Carmellini M

    更新日期:2009-06-27 00:00:00

  • The effect of idarubicin monoclonal antibody treatment of first-set rejection of murine skin allografts.

    abstract::Idarubicin was conjugated to anti-Ly-2.1 and anti-L3T4 monoclonal antibodies. These conjugates were used to investigate their effects on skin graft survival in mice. Several donor and recipient combinations were used so that the effect of individual or mixtures of conjugates on class I and class II antigenic differenc...


    pub_type: 杂志文章


    authors: Smyth MJ,McKenzie IF,Pietersz GA

    更新日期:1989-07-01 00:00:00

  • De novo sirolimus-based immunosuppression after liver transplantation for hepatocellular carcinoma: long-term outcomes and side effects.

    abstract:BACKGROUND:We report long-term outcomes and side effects after transplantation for hepatocellular carcinoma (HCC) using de novo, sirolimus-based immunosuppression (IS). METHODS:A total of 70 patients with HCC (mean age: 54.4+/-7 years, female/male: 12/58) were transplanted and included in the study. Immunosuppression ...


    pub_type: 杂志文章


    authors: Toso C,Meeberg GA,Bigam DL,Oberholzer J,Shapiro AM,Gutfreund K,Ma MM,Mason AL,Wong WW,Bain VG,Kneteman NM

    更新日期:2007-05-15 00:00:00

  • Donor species complement after liver xenotransplantation. The mechanism of protection from hyperacute rejection.

    abstract::Hamster hearts transplanted into stable rat recipients of hamster livers (OLT rats) were hyperacutely rejected after transfer with unaltered rat antihamster hyperimmune serum (HS). This was followed by immediate liver xenograft rejection in 4 of 5 rats. In contrast, simple heat inactivation of the rat HS resulted in p...


    pub_type: 杂志文章


    authors: Valdivia LA,Fung JJ,Demetris AJ,Celli S,Pan F,Tsugita M,Starzl TE

    更新日期:1994-03-27 00:00:00

  • Evidence that the immunosuppressive effects of FK506 and cyclosporine are identical.

    abstract::The fungal metabolite FK506 was discovered because it shared an important property, the ability to inhibit production of IL-2, with another well-known immunosuppressive fungal metabolite, CsA. FK506 has, since its isolation, also been shown to share other immunosuppressive effects with CsA. This study was performed to...


    pub_type: 杂志文章


    authors: Johansson A,Möller E

    更新日期:1990-12-01 00:00:00