Abstract:
:Pulmonary endothelium is considered the compartment most susceptible to preservation damage. This investigation was designed to analyze the efficacy of an original, University of Parma low-potassium-albumin solution (SPAL UP) on cultured human pulmonary artery endothelial cells (HPAEC) and to compare its effects with those of University of Wisconsin solution (UW) and Euro-Collins solution (EC). Cryopreserved HPAEC tertiary cultures were inoculated at the density of 5000 cells/cm2 in 9-cm2 well-plates; subcultures were then incubated at 10 degrees C for 6 hr and 16 hr in 2 ml/well of SPAL UP, UW, and EC. The HPAEC viability after incubation was assessed by evaluating the total protein content and the expression of cytotoxicity, and by analyzing the rate of protein synthesis and expression of cellular functionality after stress. Results after 6 hr of preservation showed that SPAL UP had a less significant cytotoxic effect than EC, exerted a less depressing effect on cellular metabolism, and enhanced functional recovery of endothelial cells compared with UW. At the second time interval (16 hr), SPAL UP provided a less cytotoxic effect than UW; besides, SPAL UP-induced cytotoxicity was similar to that of warm control. In conclusion, in vitro preliminary data regarding the use of SPAL UP in HPAEC preservation suggest its suitability as solution for prolonged lung protection.
journal_name
Transplantationjournal_title
Transplantationauthors
Spaggiari L,Rusca M,Carbognani P,Alfieri R,Urbani S,Cattelani L,Petronini P,Solli P,Borghetti AF,Bobbio Pdoi
10.1097/00007890-199611150-00036subject
Has Abstractpub_date
1996-11-15 00:00:00pages
1369-71issue
9eissn
0041-1337issn
1534-6080journal_volume
62pub_type
杂志文章相关文献
TRANSPLANTATION文献大全abstract::Acute graft-versus-host disease (GVHD) has recently been associated with endothelial cell injury. The potential clinical significance was explored here in an autopsy review. Thirty-seven allogeneic bone marrow recipients were identified in the autopsy files at The Johns Hopkins Hospital with no evidence of systemic in...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199401000-00016
更新日期:1994-01-01 00:00:00
abstract::The glycosaminoglycan hyaluronan is not only involved in cellular differentiation and migration but may also play a role in several inflammatory diseases. We have previously demonstrated an increased local production of hyaluronan in chronically rejected kidneys and the correspondingly affected renal vessels. In this ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199306000-00025
更新日期:1993-06-01 00:00:00
abstract::The ability of the popliteal lymph node assay (PLNA) To detect host-versus-graft activity in response to H-Y antigens was investigated in the rat by using presensitized recipients. It was shown that significant popliteal lymph node (PLN) enlargement in response to H-Y-incompatible lymphocytes from syngeneic donors cou...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198103000-00013
更新日期:1981-03-01 00:00:00
abstract::Natural killer (NK) cells effect hybrid resistance, in which parental hematopoietic cell grafts are rejected by F1 recipients. NK cells can also resist engraftment of fully MHC-mismatched allogeneic marrow. However, studies of NK cell-mediated alloresistance have relied on short-term proliferation, colony, or survival...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199601150-00024
更新日期:1996-01-15 00:00:00
abstract::Moderate hyperhomocysteinemia, an independent cardiovascular risk factor, has been reported in renal transplant recipients. In the present study, plasma concentrations of total homocysteine were significantly increased in 120 renal transplant recipients as compared with 60 healthy controls (19.0 +/- 6.9 vs. 11.6 +/- 2...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199602150-00034
更新日期:1996-02-15 00:00:00
abstract:BACKGROUND:Renal allografts were performed in rhesus monkeys using FN18-CRM9, a potent immunotoxin capable of depleting T cells to less than 1% of baseline levels in blood and lymph nodes, as a preparative agent. We have recently reported that animals pretreated with FN18-CRM9 1 week before transplantation without furt...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199705150-00023
更新日期:1997-05-15 00:00:00
abstract:BACKGROUND:To define whether immunosuppressive agents that block the interleukin (IL)-2 pathway could prevent activation-induced cell death of activated T cells in the graft, we measured expression of IL-2, IL-2 receptor alpha chain (CD25), IL-15, Fas, and Fas ligand by real time reverse transcription-polymerase chain ...
journal_title:Transplantation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/01.TP.0000063937.53702.97
更新日期:2003-05-27 00:00:00
abstract:BACKGROUND:Hemodynamic monitoring using transesophageal echocardiography (TEE) in patients with signs of portal hypertension undergoing orthotopic liver transplantation (OLT) carries potential risk of esophageal and gastric variceal hemorrhage. The aim of our retrospective analysis was to evaluate the safety of intraop...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0b013e31825475c2
更新日期:2012-07-27 00:00:00
abstract:BACKGROUND:The use of urine flow cytometry (UFC) as a noninvasive tool for the diagnosis of acute and chronic rejection of the renal allograft has been previously reported. METHODS:We analyzed the expression of various cell surface antigens during a 30-day period after the diagnosis and treatment of 24 acute rejection...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200105150-00024
更新日期:2001-05-15 00:00:00
abstract::This study was undertaken to examine the humoral immune response against endothelial antigens of the donor kidney in human renal allograft recipients. Sera from 61 transplant recipients who received 62 grafts were studied for the presence of circulating endothelial antibodies (CEAb) using an indirect immunofluorescenc...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-197903000-00007
更新日期:1979-03-01 00:00:00
abstract:BACKGROUND:It has been postulated that chimerism after transplantation might promote graft acceptance. In the present study, we prospectively assessed blood chimerism in 10 lung transplant recipients during the first posttransplant year and investigated whether chimerism was associated with an immunologically stable si...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199707150-00030
更新日期:1997-07-15 00:00:00
abstract::Allotransplantation into immunosuppressed individuals results in long-term survival of grafts. However, the grafts are damaged, probably at many stages before, during and after implantation. The hypothesis to be presented is that release of antigens and autoantigens from the chronically damaged graft results in breaki...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/01.tp.0000153294.38939.5f
更新日期:2005-02-15 00:00:00
abstract::Renal function was observed retrospectively in a population of 228 adults who underwent a cardiac allograft at the University of Pittsburgh from June 1980 through June 1987, survived a minimum of one year, and received cyclosporine. Renal function was determined by serial measurement of serum creatinine concentration....
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199010000-00012
更新日期:1990-10-01 00:00:00
abstract::Living donor renal allograft survival is superior to that achieved from deceased donors, although graft outcome is suboptimal in some of these patients. In an effort to identify the subset of patients at high risk for poor outcomes we studied donor risk factors in 248 living kidney donor-recipient pairs. Unadjusted do...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/01.tp.0000256284.78721.ba
更新日期:2007-03-15 00:00:00
abstract:BACKGROUND:HLA-specific antibodies detected by solid phase assays are increasingly used to define unacceptable HLA antigen mismatches (UAM) before renal transplantation. The accuracy of this approach is unclear. METHODS:Day of transplant sera from 211 complement-dependent cytotoxicity crossmatch-negative patients were...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/TP.0000000000002129
更新日期:2018-06-01 00:00:00
abstract:BACKGROUND:Successful kidney transplantation normalizes elevated proatrial natriuretic peptide (proANP) plasma concentrations of renal failure patients in the early posttransplant period. We evaluated plasma and urinary proANP fragments in the late posttransplant period. METHODS:Immunoreactive proANP(1-30) and proANP(...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200107150-00018
更新日期:2001-07-15 00:00:00
abstract::Allograft rejection results from a complex process involving both the innate and acquired immune systems. The innate immune system predominates in the early phase of the allogeneic response, during which chemokines and cell adhesion play essential roles, not only for leukocyte migration into the graft but also for fac...
journal_title:Transplantation
pub_type: 杂志文章,评审
doi:10.1097/00007890-200205150-00001
更新日期:2002-05-15 00:00:00
abstract::The effect of cyclosporine on hepatic ischemia was investigated. Hepatic ischemia was produced for 90 min in mongrel dogs. Experimental dogs were divided into three groups as follows: group A (control group), group B (CsA pretreatment group), group C (CsA posttreatment group). CsA was administered at a dose of 10 mg/k...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199107000-00024
更新日期:1991-07-01 00:00:00
abstract::Cultured neonatal rat islets were transplanted across six strain combinations into nonimmunosuppressed allogeneic recipients. Islets were isolated nonenzymatically by an in vitro method and were cultured at 37 degrees C in 5% CO2 in air for 10 days prior to transplant. Transplants to nondiabetic recipients across four...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198712000-00003
更新日期:1987-12-01 00:00:00
abstract::Two patients with Fanconi's anemia treated for 5 years with oxymetholone developed hepatic function abnormalities in association with hepatic tumors demonstrated by isotope liver-spleen scan or abdominal echogram. The lesions resolved over a period of 26 months after allogeneic marrow transplantation, and the patients...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198405000-00006
更新日期:1984-05-01 00:00:00
abstract::We initiated a policy of using RSLT in critically ill patients in June of 1988. Since that time we have performed 30 RSLTs in 29 patients, including 28 children and 1 adult. The mean age of the children was 27 months (range 1 month to 10 years) with 14 (52%) being 1 year of age or less. The mean weight was 11.3 kg (ra...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199202010-00023
更新日期:1992-02-01 00:00:00
abstract::Disseminated varicella-zoster (VZV) infection is a rare complication after renal allotransplantation in adults. We report four patients, among them one with combined VZV and cytomegalovirus infection. The main complications were hepatitis, pneumonitis, and disseminated intravascular coagulation. A review of the litera...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200202270-00023
更新日期:2002-02-27 00:00:00
abstract::The pharmacologic modulation of the platelet-activating factor antagonist WEB 2086BS on the release of metabolites of the arachidonic acid and the cytokine TNF alpha was investigated in an ex vivo xenograft model of hyperacute rejection. Pig kidneys were perfused for 60 min in a perfusion system with oxygenated hepari...
journal_title:Transplantation
pub_type: 杂志文章
doi:
更新日期:1994-02-01 00:00:00
abstract::Using liposomes, multilamellar lipid vesicles (MLV), we have found that liposome-incorporated cyclosporine is not only less toxic but also more effective in prolonging survival of cardiac allografts in mice. Following intravenous injection of liposome-incorporated drug, cyclosporine levels in blood were shown to decre...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199111000-00002
更新日期:1991-11-01 00:00:00
abstract:BACKGROUND:Induction immunosuppressive therapy with the anti-T-cell antibody Thymoglobulin decreases the incidence of acute rejection in adult kidney transplant (KTx) recipients, but limited data are available for pediatric KTx recipients. METHODS:We conducted a historical cohort study to compare rates of survival, re...
journal_title:Transplantation
pub_type: 临床试验,杂志文章
doi:10.1097/01.tp.0000158325.12837.a2
更新日期:2005-04-27 00:00:00
abstract::Late deaths (after more than 1 year) after liver transplantation were analyzed in a series of 464 consecutive patients who received liver grafts between 1982 and 1993. Recipients who survived the first posttransplant year (n = 365) had actuarial 5- and 10-year survival rates of 92% and 84%, respectively. Thirty-five p...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199605150-00016
更新日期:1996-05-15 00:00:00
abstract::Vascularized xenografts are rejected acutely and hyperacutely in concordant or discordant combinations, respectively. We investigated the impact of the donor-recipient combination on the rejection of arterial xenografts, analyzing the cellular and extracellular matricial compartments. Aortic xenografts were performed ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199609270-00017
更新日期:1996-09-27 00:00:00
abstract::The potential usefulness of verapamil, a calcium channel blocker to renal allograft preservation, was investigated using the isolated perfused rat kidney. Two models of ischemic injury were used. In the first model, rat kidneys were exposed to 40 min of 37 degrees C ischemia on the perfusion circuit. Addition of verap...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-198512000-00004
更新日期:1985-12-01 00:00:00
abstract::The effects of FK506 on exocrine function of the pancreas in Sprague-Dawley rats were examined and compared with those of cyclosporine. FK506 did not affect the levels of amylase and lipase in the pancreas of rats given the drug orally in a daily dose of 1 or 5 mg/kg for 2 weeks. Furthermore, the pancreas of the rats ...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-199211000-00023
更新日期:1992-11-01 00:00:00
abstract:BACKGROUND:In a pilot trial we evaluated the toxicity and efficacy of sirolimus (rapamycin) as second-line therapy for the treatment of acute graft-versus-host disease (GVHD) in 21 patients (1-46 years of age) after allogeneic hematopoietic stem cell transplantation (HSCT). METHODS:All patients were treated with methy...
journal_title:Transplantation
pub_type: 杂志文章
doi:10.1097/00007890-200112270-00010
更新日期:2001-12-27 00:00:00