Abstract:
:In previous studies we demonstrated that insulin-like growth factor I (IGF-I) induces pituitary vasoactive intestinal peptide (VIP) gene expression and secretion, and that IGF-I-induced prolactin (PRL) release is mediated by VIP. In this study, we investigate the mitotropic action of IGF-I and VIP on pituitary lactotropes, and their possible interplay in this effect. Cultured male rat pituitary cells were treated with rhIGF-I (10(-7)M) and/or VIP (10(-7)M) for 48 h. 5-Bromo-2'-deoxyuridine (BrdU) (10 microM) was added for labeling proliferation of pituitary cells. BrdU-labeling indices indicative of the proliferation rate of lactotropes were determined by double-labeling immunofluorescence staining for PRL and BrdU. Treatment with either IGF-I or VIP increased BrdU-labeling indices of lactotropes, but there was no further increase upon combined incubation with both factors, suggesting an interaction between the signal transduction pathways of IGF-I and VIP. VIP antiserum partially suppressed IGF-I-induced BrdU-labeling indices of lactotropes. We also investigated the intracellular signal transduction pathways in the action of IGF-I and VIP on the proliferation of lactotropes. Treatment of pituitary cells with an inhibitor of the mitogen-activated protein kinase (MAPK) pathway completely abolished IGF-I-induced lactotrope proliferation, whereas it partially suppressed VIP-induced BrdU-labeling indices. The protein kinase A (PKA) inhibitor, which abolished the mitogenic action of VIP, markedly suppressed IGF-I-induced lactotrope proliferation. These results indicate that both IGF-I and VIP stimulate lactotrope proliferation, and that IGF-I-induced lactotrope proliferation is partially mediated by VIP produced locally. Also, this study suggests that interactions between MAPK and cyclic adenosine 3',5'-monophosphate-PKA signaling pathways are implicated in the lactotrope proliferation induced by IGF-I and VIP.
journal_name
Neuroendocrinologyjournal_title
Neuroendocrinologyauthors
Fernández M,Sánchez-Franco F,Palacios N,Sánchez I,Villuendas G,Cacicedo Ldoi
10.1159/000070900subject
Has Abstractpub_date
2003-05-01 00:00:00pages
341-52issue
5eissn
0028-3835issn
1423-0194pii
70900journal_volume
77pub_type
杂志文章abstract::Pregnancy and parturition are associated with opioid-mediated elevations in maternal pain thresholds. This analgesia is subserved by a spinal cord dynorphin/kappa-opiate receptor system. During gestation, elevated pain thresholds are paralleled by a significant increase in the content of dynorphin (1-17 and 1-8) in th...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126555
更新日期:1993-09-01 00:00:00
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journal_title:Neuroendocrinology
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doi:10.1159/000472255
更新日期:2017-01-01 00:00:00
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doi:10.1159/000327430
更新日期:2011-01-01 00:00:00
abstract::Many aspects of reproductive neuroendocrine function and sexual behavior are responsive to both androgens and estrogens, suggesting that receptors for these steroid hormones may reside within single cells in brain regions that control reproductive function. We determined the distribution of estrogen receptor-containin...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000127039
更新日期:1995-11-01 00:00:00
abstract::Pituitary oxytocin (OT) secretion is inversely related to saline consumption in several experimental models of sodium appetite in rats. Because systemic OT administration does not inhibit sodium appetite, release of OT as a neurotransmitter within the brain, coincident with its secretion from the pituitary, may be rel...
journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000126236
更新日期:1992-08-01 00:00:00
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journal_title:Neuroendocrinology
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2002-02-01 00:00:00
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journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000122750
更新日期:1978-01-01 00:00:00
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journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000054405
更新日期:1999-02-01 00:00:00
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journal_title:Neuroendocrinology
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1997-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1159/000122870
更新日期:1979-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1159/000123261
更新日期:1981-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1159/000126869
更新日期:1995-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1159/000125232
更新日期:1989-09-01 00:00:00
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journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000338794
更新日期:2012-01-01 00:00:00
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journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124668
更新日期:1986-01-01 00:00:00
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journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123576
更新日期:1983-11-01 00:00:00
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journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000125612
更新日期:1990-10-01 00:00:00
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journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124839
更新日期:1987-10-01 00:00:00
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journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000122884
更新日期:1979-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1986-01-01 00:00:00
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更新日期:1977-01-01 00:00:00
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更新日期:1995-06-01 00:00:00
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journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124484
更新日期:1986-01-01 00:00:00
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journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000124039
更新日期:1984-12-01 00:00:00
abstract::Recent work has demonstrated that the brain has the capacity to synthesize impressive amounts of the gases nitric oxide (NO) and carbon monoxide (CO). There is growing evidence that these gaseous molecules function as novel neural messengers in the brain. This article reviews the pertinent literature concerning the pu...
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pub_type: 杂志文章,评审
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更新日期:1997-06-01 00:00:00
abstract::Vasoactive intestinal peptide (VIP), a prolactin (PRL)-releasing factor, has been shown to be synthesized within the anterior pituitary. To test the hypothesis that estrogens increase PRL secretion, at least in part, by stimulating VIP secretion, the concentrations of VIP, peptide histidine isoleucine (PHI) and prepro...
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pub_type: 杂志文章
doi:10.1159/000125622
更新日期:1990-11-01 00:00:00
abstract::Previous studies indicate that 7 days after testicular administration of naloxone, serum testosterone (T) concentration and basal T secretion in vitro decrease significantly. In neonatal rats, short-term (2 h) intratesticular treatment with 0.3 micrograms (D-Met2-Pro5)-enkephalinamide suppresses steroidogenesis. In th...
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pub_type: 杂志文章
doi:10.1159/000126242
更新日期:1992-09-01 00:00:00
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journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000125773
更新日期:1991-06-01 00:00:00
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journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000511811
更新日期:2020-09-25 00:00:00
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journal_title:Neuroendocrinology
pub_type: 杂志文章
doi:10.1159/000123976
更新日期:1984-08-01 00:00:00