Abstract:
:Efficient immunoglobulin (Ig) production was induced when human B cells were cultured with autologous T cells activated by immobilized CD3 mAb in cultures supplemented with IL-2. Negatively purified B cells or B cells positively selected with mAb to CD19, CD21 or CD72 surface antigens produced IgM, IgG and IgA, whereas B cells selected for surface IgD or IgM produced predominantly IgM indicating that little or no isotype switching was occurring. Results are compared with reports describing high levels of mu to gamma and mu to alpha switching in single B cell systems. The limited proliferation of B cells in our culture system may account for the difference. When untreated T and B cells were cultured together in the presence of immobilized CD3 mAb, B cell numbers peaked at 6-10 days whereas T cells continued to proliferate maximally. All 52 T cell clones tested induced the production of IgM, IgG and IgA from unselected or CD19 selected B cells, but efficiency of production of Ig overall and of the different isotypes varied with different T clones. All T clones which induced high IgM, IgG and IgA production induced IgE production too, but some less active T clones also induced IgE production under non-switching conditions indicating that direct contact with activated T clone cells efficiently induces IgE as well as IgG and IgA production from B cells already expressing these isotypes. Less Ig was produced with optimal numbers of untreated T clone cells than with X-irradiated cells, confirming that proliferating T cells can inhibit as well as activate Ig production from B cells.
journal_name
Immunobiologyjournal_title
Immunobiologyauthors
Ling NR,Lowe JLdoi
10.1016/S0171-2985(11)80415-8subject
Has Abstractpub_date
1993-12-01 00:00:00pages
436-47issue
5eissn
0171-2985issn
1878-3279pii
S0171-2985(11)80415-8journal_volume
189pub_type
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