Abzyme generation using an anti-idiotypic antibody as the "internal image" of an enzyme active site.

abstract：:The wet oxidation pretreatment (water, oxygen, elevated temperature, and pressure) of softwood (Picea abies) was investigated for enhancing enzymatic hydrolysis. The pretreatment was preliminarily optimized. Six different combinations of reaction time, temperature, and pH were applied, and the compositions of solid an...

journal_title：Applied biochemistry and biotechnology

authors： Palonen H,Thomsen AB,Tenkanen M,Schmidt AS,Viikari L

更新日期：2004-04-01 00:00:00

abstract：:A proteinaceous glycopeptide was isolated from the posterior salivary gland (PSG) of Sepia pharaonis by gel (Sephadex G-100) filtration chromatography and purified by reversed-phase high-performance liquid chromatography (RP-HPLC). Among the collected fractions, fraction 12 showed a retention time (RT) of 31 min. The ...

journal_title：Applied biochemistry and biotechnology

authors： Karthik R,Saravanan R,Ebenezar KK,Sivamalai T

更新日期：2015-02-01 00:00:00

abstract：:Nanotechnology is an emerging technology which is an amalgamation of different aspects of science and technology that includes disciplines such as electrical engineering, mechanical engineering, biology, physics, chemistry, and material science. It has potential in the fields of information and communication technolog...

journal_title：Applied biochemistry and biotechnology

authors： Chakraborty M,Jain S,Rani V

更新日期：2011-11-01 00:00:00

abstract：:The effect of methanol on trichloroethylene (TCE) degradation by mixed and pure methylotrophic cultures was examined in batch culture experiments. Methanol was found to relieve growth inhibition of Methylosinus trichosporium (OB3b) at high (14 mg/L) TCE concentrations. Degradation of TCE was determined by both radiola...

journal_title：Applied biochemistry and biotechnology

authors： Eng W,Palumbo AV,Sriharan S,Strandberg GW

更新日期：1991-04-01 00:00:00

abstract：:Cross-linking of enzyme aggregates from recombinant Geotrichum sp. lipase based on polyethylenimine (PEI) was applied to hydrolyze fish oil for enrichment of polyunsaturated fatty acids successfully. Through acetone precipitation and cross-linking of physical aggregates using glutaraldehyde in the presence of PEI, fir...

journal_title：Applied biochemistry and biotechnology

authors： Yan J,Gui X,Wang G,Yan Y

更新日期：2012-02-01 00:00:00

abstract：:The genes encoding purine nucleoside phosphorylase (PNPase), uridine phosphorylase (UPase), and thymidine phosphorylase (TPase) from Escherichia coli K12 were cloned respectively into expression vector pET-11a or pET-28a. The recombinant plasmids were transformed into the host strain E. coli BL21(DE3) to construct fou...

journal_title：Applied biochemistry and biotechnology

authors： Ge C,OuYang L,Ding Q,Ou L

更新日期：2009-10-01 00:00:00

abstract：:Corn stover has potential as a resource for both fiber and chemical needs if separation strategies can be developed to deal with its heterogeneity. Relative hydrolysis characteristics were assessed for pith (sclerenchyma and parenchyma) and fiber (collenchyma) tissue fractions derived from mechanical separation of cor...

journal_title：Applied biochemistry and biotechnology

authors： Bootsma JA,Shanks BH

更新日期：2005-04-01 00:00:00

abstract：:The three-dimensional distribution (x, y, and z) of ultrasound-induced microalgal cell disruption in a sonochemical reactor was predicted by solving the Helmholtz equation using a three-dimensional acoustic module in the COMSOL Multiphysics software. The simulated local ultrasound pressure at any given location (x, y,...

journal_title：Applied biochemistry and biotechnology

authors： Wang M,Yuan W,Hale A

更新日期：2016-03-01 00:00:00

abstract：:Based on the principle of self-coagulation of microorganisms, the flocculant-producing denitrifying bacterial TN-14 sludge was added to the continuous-flow reactor for treating domestic sewage. The bacterial TN-14 sludge acted as the main seed sludge to promote the rapid formation of aerobic granular sludge. The sludg...

journal_title：Applied biochemistry and biotechnology

authors： Xin X,Lu H,Yao L,Leng L,Guan L

更新日期：2017-01-01 00:00:00

abstract：:Laccase is one of the lignolytic enzymes found in liquid cultures of the fungus Coriolus zonatus in defined medium. The enzyme was isolated from culture liquid and characterized. Laccase from C. zonatus is a single-chain protein with a molecular mass of 60 kDa. Carbohydrate moiety of enzyme consisted of mannose, galac...

journal_title：Applied biochemistry and biotechnology

authors： Koroljova OV,Stepanova EV,Gavrilova VP,Biniukov VI,Jaropolov AI,Varfolomeyev SD,Scheller F,Makower A,Otto A

更新日期：1999-02-01 00:00:00

abstract：:Fatty acid methyl esters (FAMEs) show large potential applications as diesel substitutes, also known as biodiesel fuel. Biodiesel fuel as renewable energy is an alternative that can reduce energy dependence on petroleum as well as air pollution. Several processes for the production of biodiesel fuel have been develope...

journal_title：Applied biochemistry and biotechnology

authors： Jeong GT,Park DH,Kang CH,Lee WT,Sunwoo CS,Yoon CH,Choi BC,Kim HS,Kim SW,Lee UT

更新日期：2004-04-01 00:00:00

abstract：:Residue of chili plants left in the field after harvesting is a major lignocellulosic resource that is underexploited. India has over 0.6 million tons of this residue available as surplus annually which projects it as a potent feedstock for conversion to bioethanol. The cellulose, hemicellulose and lignin content of t...

journal_title：Applied biochemistry and biotechnology

authors： Preeti VE,Sandhya SV,Kuttiraja M,Sindhu R,Vani S,Kumar SR,Pandey A,Binod P

更新日期：2012-07-01 00:00:00

abstract：:Microbial oil accumulation via oleaginous fungi has some potential benefits because filamentous fungi can form pellets during cell growth and these pellets are easier to harvest from the culture broth than individual cells. This research studied the effect of various culture conditions on the pelletized cell growth of...

journal_title：Applied biochemistry and biotechnology

authors： Xia C,Wei W,Hu B

更新日期：2014-04-01 00:00:00

abstract：:Sorbitol, a polyol found in many fruits, is attracting increasing industrial interest as a sweetener, humectant, texturizer, and softener. It is principally produced by chemical means. The bacterium Zymomonas mobilis is able to produce sorbitol together with gluconic acid from fructose and glucose, respectively. This ...

journal_title：Applied biochemistry and biotechnology

authors： Jonas R,Silveira MM

更新日期：2004-07-01 00:00:00

abstract：:(R)-2-Phenoxy-propionic acid methyl ester (PPAM) is an important chiral precursor of aryloxy phenoxy propionate herbicides. The covalent immobilization of lipase from Aspergillus oryzae WZ007 and the catalysis of enantioselective (R, S)-PPAM resolution by the immobilized A. oryzae lipase (AOL) were investigated in thi...

journal_title：Applied biochemistry and biotechnology

authors： Zhong W,Zhang M,Li X,Zhang Y,Wang Z,Zheng J

更新日期：2020-03-01 00:00:00

abstract：:The role of selenium (Se) supplementation on glutathione, a potent intracellular redox buffer, and trehalose, a well-known stress protectant molecule, was studied. The amount of glutathione decreased significantly while that of trehalose showed a minor decrease in the cells grown in Se-supplemented medium. After heat ...

journal_title：Applied biochemistry and biotechnology

authors： Sharma SC,Anand MS

更新日期：2006-04-01 00:00:00

abstract：:The changes in total phenolics, flavonoids, tannins, valerenic acid, and antioxidant activity were assessed in 25 populations of Valeriana jatamansi sampled from 1200 to 2775 m asl and four habitat types of Uttarakhand, West Himalaya. Significant (p < 0.05) variations in total phenolics, flavonoids, valerenic acid, an...

journal_title：Applied biochemistry and biotechnology

authors： Jugran AK,Bahukhandi A,Dhyani P,Bhatt ID,Rawal RS,Nandi SK

更新日期：2016-07-01 00:00:00

abstract：:As a part of our study on microbial heterogeneity in subsurface environments, we have examined the microbial respiration of sediment samples obtained from a coastal site near Oyster, VA. The sediments at the site are unconsolidated, fine to coarse beach sand and gravel. A Columbus Instruments Micro-Oxymax Respirometer...

journal_title：Applied biochemistry and biotechnology

authors： Chapatwala KD,Babu GR,Vijaya OK,Armstead E,Palumbo AV,Zhang C,Phelps TJ

更新日期：1996-04-01 00:00:00

abstract：:One of the major limitations of effective nonviral gene carriers is their potential high cytotoxicity. Conjugation of polyethylene glycol (PEG) to polymers is a common approach to decrease toxicity and improve biodistribution. The aim of this study was to evaluate the effect of PEGylation on generation 5 polypropyleni...

journal_title：Applied biochemistry and biotechnology

authors： Hashemi M,Ayatollahi S,Parhiz H,Mokhtarzadeh A,Javidi S,Ramezani M

更新日期：2015-09-01 00:00:00

abstract：:Environmental conditions affect the production of extracellular polysaccharide by Azotobacter chroococcum ATCC 4412. Production of exocellular polymer from a variety of carbon sources depended on the air flow rate. A high sucrose concentration in medium (8%) markedly favored expopolysaccharide production, which reache...

journal_title：Applied biochemistry and biotechnology

authors： De la Vega MG,Cejudo FJ,Paneque A

更新日期：1991-09-01 00:00:00

abstract：:Batch, fed-batch, and semicontinuous fermentation processes were used for the production of xylitol from sugarcane bagasse hemicellulosic hydrolysate. The best results were achieved by the semicontinuous fermentation process: a xylitol yield of 0.79 g/g with an efficiency of 86% and a volumetric productivity of 0.66 g...

journal_title：Applied biochemistry and biotechnology

authors： Rodrigues DC,Silva SS,Prata AM,Felipe MG

更新日期：1998-04-01 00:00:00

abstract：:The ability of poly(ethylene glycol) (PEG) to protect enzymatic peroxidase activity was determined for horseradish peroxidase (HRP), versatile peroxidase (VP), commercial Coprinus peroxidase (BP), and chloroperoxidase (CPO). The operational stability measured as the total turnover number was determined for the four pe...

journal_title：Applied biochemistry and biotechnology

authors： Juarez-Moreno K,Ayala M,Vazquez-Duhalt R

更新日期：2015-11-01 00:00:00

abstract：:Streptomyces badius DB-1 produces α-amylase extracellularly, and its production was enhanced 5.1-fold (from 9.47 ± 0.51 to 48.23 ± 1.45 U mL-1) due to optimization by one-variable-at-a-time and statistical approaches. Soluble starch emerged as the most influential factor that strongly affected enzyme production. The p...

journal_title：Applied biochemistry and biotechnology

authors： Shivlata L,Satyanarayana T

更新日期：2017-04-01 00:00:00

abstract：:Non-Systematic Evolution of Ligands by EXponential enrichment (SELEX)selection of aptamers, a novel technology for aptamer selection from libraries of random DNA (or RNA) sequences, involves repetitive steps of partitioning without polymerase chain reaction (PCR) amplification between them. This selection is based on ...

journal_title：Applied biochemistry and biotechnology

authors： Yu X,Yu Y

更新日期：2014-08-01 00:00:00

abstract：:Lignocellulose-decaying fungal bioplatforms available are not commercially accessible and are limited to short-term use. In this study, those limitations were overcome by developing a platform using water-soaked rice straw (RS) biodegraded by irradiation-based fungal pretreatment (IBFP). This eco-friendly system incre...

journal_title：Applied biochemistry and biotechnology

authors： Bak JS

更新日期：2015-12-01 00:00:00

abstract：:The performance of immobilized Bifidobacterium longum in sodium alginate beads and on a spiral-sheet bioreactor for the production of lactic acid from cheese whey was evaluated. Lactose utilization and lactic acid yield of B. longum were compared with those of Lactobacillus helveticus. B. longum immobilized in sodium ...

journal_title：Applied biochemistry and biotechnology

authors： Shahbazi A,Mims MR,Li Y,Shirley V,Ibrahim SA,Morris A

更新日期：2005-04-01 00:00:00

abstract：:The RNA-guided endonuclease clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) derived from CRISPR systems is a simple and efficient genome-editing technology applied to various cell types and organisms. So far, the extensive approach to detect the cleavage activity of custo...

journal_title：Applied biochemistry and biotechnology

authors： Yang Y,Liu S,Cheng Y,Nie L,Lv C,Wang G,Zhang Y,Hao L

更新日期：2016-10-01 00:00:00

abstract：:In recent years, the concept of producing biodiesel from renewable lipid sources has regained international attention. In Brazil, a national program was launched in 2002 to evaluate the technical, economic, and environmental competitiveness of biodiesel in relation to the commercially available diesel oil. Several res...

journal_title：Applied biochemistry and biotechnology

authors： Ramos LP,Wilhelm HM

更新日期：2005-04-01 00:00:00

abstract：:The vegetative insecticidal proteins (VIPs) of Bacillus thuringiensis (Bt) have a broad-spectrum insecticidal activity against Lepidopteran pests and no cross-resistance with the insecticidal crystal protein Cry protein. So there are great potentials for the control of agricultural pests and the resolution of resistan...

journal_title：Applied biochemistry and biotechnology

authors： Chi B,Li H,Zhang J,Wei P,Gao J,Liu R

更新日期：2019-04-01 00:00:00

abstract：:Milk is a natural nutraceutical produced by mammals. The nanovesicles of milk play a role in horizontal gene transfer and confer health-benefits to milk consumers. These nanovesicles contain miRNA, mRNA, and proteins which mediate the intercellular communication. In this work, we isolated and characterized the buffalo...

journal_title：Applied biochemistry and biotechnology

authors： Baddela VS,Nayan V,Rani P,Onteru SK,Singh D

更新日期：2016-02-01 00:00:00