Isolation, purification, and characterization of avian antimicrobial glycopeptide from the posterior salivary gland of Sepia pharaonis.

Abstract:

:A proteinaceous glycopeptide was isolated from the posterior salivary gland (PSG) of Sepia pharaonis by gel (Sephadex G-100) filtration chromatography and purified by reversed-phase high-performance liquid chromatography (RP-HPLC). Among the collected fractions, fraction 12 showed a retention time (RT) of 31 min. The total protein and neutral sugar contents of the purified glycopeptide were recorded as 68.14 and 2.95 mg, respectively. The molecular weight of the purified glycopeptide was found to be ~50 kDa. The infrared (IR) and circular dichroism (CD) spectroscopy confirmed the presence of peptide and secondary structure in the purified glycopeptide. The antibacterial activity of the purified glycopeptide against avian bacterial strains was also determined. Gas chromatography-mass spectrometry (GC-MS) of the purified glycopeptide revealed the likely compounds for the antibacterial activity such as 22, 23-dibromostigmasterol acetate, 3-methyl 2-(2-oxypropyl) furan, and 2,4,4-trimethyl-3-hydroxymethyl-5A-(3-methyl-but-2-enyl)-cyclohexene. These three compounds found in the purified glycopeptide could be responsible for the antibacterial activity against the avian pathogens. The results of this study suggest that the purified glycopeptide from the PSG of S. pharaonis could be an antibacterial agent against avian bacterial pathogens.

authors

Karthik R,Saravanan R,Ebenezar KK,Sivamalai T

doi

10.1007/s12010-014-1370-8

subject

Has Abstract

pub_date

2015-02-01 00:00:00

pages

1507-18

issue

3

eissn

0273-2289

issn

1559-0291

journal_volume

175

pub_type

杂志文章
  • Statistical analysis and modeling of pelletized cultivation of Mucor circinelloides for microbial lipid accumulation.

    abstract::Microbial oil accumulation via oleaginous fungi has some potential benefits because filamentous fungi can form pellets during cell growth and these pellets are easier to harvest from the culture broth than individual cells. This research studied the effect of various culture conditions on the pelletized cell growth of...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0759-8

    authors: Xia C,Wei W,Hu B

    更新日期:2014-04-01 00:00:00

  • Biogas by semi-continuous anaerobic digestion of food waste.

    abstract::The semi-continuous anaerobic digestion of food waste was investigated in 1-L and 20-L continuously stirred tank reactors (CSTRs), to identify the optimum operation condition and the methane production of the semi-continuous anaerobic process. Results from a 1-L digester indicated that the optimum organic loading rate...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-015-1559-5

    authors: Zhang C,Su H,Wang Z,Tan T,Qin P

    更新日期:2015-04-01 00:00:00

  • Purification and partial characterization of an exo-polygalacturonase from Paecilomyces variotii liquid cultures.

    abstract::An extracellular polygalacturonase (PG) produced from Paecilomyces variotii was purified to homogeneity through two chromatography steps using DEAE-Fractogel and Sephadex G-100. The molecular weight of P. variotii PG was 77,300 Da by gel filtration and SDS-PAGE. PG had isoelectric point of 4.37 and optimum pH 4.0. PG ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8682-0

    authors: de Lima Damásio AR,da Silva TM,Maller A,Jorge JA,Terenzi HF,Polizeli Mde L

    更新日期:2010-03-01 00:00:00

  • Identification of heat-related ESTs in moth bean through suppression subtraction hybridization.

    abstract::Moth bean (Vigna aconitifolia (Jacq.) Marechal), an important grain-legume crop grown in hot desert regions of Thar, under scorching sun rays, was investigated for heat tolerance at molecular level. In the present study, we constructed a forward suppression subtractive hybridization (SSH) cDNA library of heat tolerant...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-1011-2

    authors: Gurjar K,Rampuria S,Joshi U,Palit P,Bhatt KV,Mohapatra T,Sharma R

    更新日期:2014-08-01 00:00:00

  • Production of insoluble exopolysaccharide of Agrobacterium sp. (ATCC 31749 and IFO 13140).

    abstract::Agrobacterium isolated from soil samples produced two extracellular polysaccharides: succinoglycan, an acidic soluble polymer, and curdlan gum, a neutral, insoluble polymer. Maize glucose, cassava glucose, and maize maltose were used in fermentation medium to produce insoluble polysaccharide. Two Agrobacterium sp. str...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/ABAB:131:1:864

    authors: Portilho M,Matioli G,Zanin GM,de Moraes FF,Scamparini AR

    更新日期:2006-03-01 00:00:00

  • Lignin Sulfonation and SO2 Addition Enhance the Hydrolyzability of Deacetylated and Then Steam-Pretreated Poplar with Reduced Inhibitor Formation.

    abstract::The merit of deacetylation of corn stover prior to pretreatment is decreasing the formation of inhibitors and improving enzyme hydrolysis, proved in dilute acid pretreatment. However, few studies are done on how deacetylation would affect bioconversion process containing steam explosion. In this study, the effect of d...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-017-2545-x

    authors: Tang Y,Dou X,Hu J,Jiang J,Saddler JN

    更新日期:2018-01-01 00:00:00

  • Expression of codon-optmized phosphoenolpyruvate carboxylase gene from Glaciecola sp. HTCC2999 in Escherichia coli and its application for C4 chemical production.

    abstract::We examined the expression of the phosphoenolpyruvate carboxylase (PEPC) gene from marine bacteria in Escherichia coli using codon optimization. The codon-optimized PEPC gene was expressed in the E. coli K-12 strain W3110. SDS-PAGE analysis revealed that the codon-optimized PEPC gene was only expressed in E. coli, and...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-9730-8

    authors: Park S,Pack SP,Lee J

    更新日期:2012-08-01 00:00:00

  • Chitosan-modified carbon nanotubes-based platform for low-density lipoprotein detection.

    abstract::We have fabricated an immunosensor based on carbon nanotubes and chitosan (CNT-CH) composite for detection of low density lipoprotein (LDL) molecules via electrochemical impedance technique. The CNT-CH composite deposited on indium tin oxide (ITO)-coated glass electrode has been used to covalently interact with anti-a...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-1179-5

    authors: Ali MA,Singh N,Srivastava S,Agrawal VV,John R,Onoda M,Malhotra BD

    更新日期:2014-10-01 00:00:00

  • Analysis of kinetic and operational parameters in a structured model for acrylic acid production through experimental design.

    abstract::In biotechnological processes, a great number of factors can influence the income productivity and conversion. Normally, it is not evident which of these factors are the most important and how they interact. In this work, multivariate analysis techniques are used as experimental design coupled to a detailed determinis...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-007-8060-8

    authors: Lunelli BH,Rivera EC,Vasco de Toledo EC,Wolf Maciel MR,Maciel Filho R

    更新日期:2008-03-01 00:00:00

  • Purification and characterization of the glucoside 3-dehydrogenase produced by a newly isolated Sphingobacterium faecium ZJF-D6 CCTCC M 2013251.

    abstract::A soluble glucoside 3-dehydrogenase (G3DH) was purified from a newly isolated Sphingobacterium faecium ZJF-D6 CCTCC M 2013251. The enzyme was purified to 35.71-fold with a yield of 41.91 % and was estimated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis with a molecular mass of 62 kDa. The sequences of ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0816-3

    authors: Zhang JF,Yang B,Chen W,Chen J

    更新日期:2014-04-01 00:00:00

  • Strategies for reducing solvent toxicity in extractive ethanol fermentation.

    abstract::Extractive fermentation is a widely preferred technique in which the products of fermentation are removed from the fermentation medium by a proper solvent, in order to avoid the inhibitory effects of the products. In this work, decanol, which has a high distribution coefficient with respect to the biocompatible solven...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02787775

    authors: Kapucu H,Mehmetoğlu U

    更新日期:1998-11-01 00:00:00

  • Modeling a protein foam fractionation process.

    abstract::A simple staged model for the protein foam fractionation process is proposed in this article. This simplified model does not detail the complex foam structure and gas-liquid hydrodynamics in the foam phase but, rather, is built on the conventional theoretical stage concept considering upward bubbles with entrained liq...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:84-86:1-9:1087

    authors: Du L,Loha V,Tanner RD

    更新日期:2000-04-01 00:00:00

  • Rheological behavior and non-enzymatic degradation of a sulfated galactan from Halymenia durvillei (Halymeniales, Rhodophyta).

    abstract::The rheological behavior of a sulfated galactan extracted from Halymenia durvillei, a red seaweed collected in the coastal waters of a small island of Madagascar (Nosy-be in Indian Ocean), was investigated in dilute and semi-dilute solutions. In dilute solution with NaCl at 0.3 M, the polysaccharide adopted a coil con...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-9605-z

    authors: Fenoradosoa TA,Laroche C,Delattre C,Dulong V,Le Cerf D,Picton L,Michaud P

    更新日期:2012-07-01 00:00:00

  • The Effect of Ionic Liquid Pretreatment on the Bioconversion of Tomato Processing Waste to Fermentable Sugars and Biogas.

    abstract::Tomato pomace is an abundant lignocellulosic waste stream from industrial tomato processing and therefore a potential feedstock for production of renewable biofuels. However, little research has been conducted to determine if pretreatment can enhance release of fermentable sugars from tomato pomace. Ionic liquids (ILs...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-016-2061-4

    authors: Allison BJ,Cádiz JC,Karuna N,Jeoh T,Simmons CW

    更新日期:2016-08-01 00:00:00

  • Optimization of biodiesel production from castor oil using response surface methodology.

    abstract::The short supply of edible vegetable oils is the limiting factor in the progression of biodiesel technology; thus, in this study, we applied response surface methodology in order to optimize the reaction factors for biodiesel synthesis from inedible castor oil. Specifically, we evaluated the effects of multiple parame...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-008-8468-9

    authors: Jeong GT,Park DH

    更新日期:2009-05-01 00:00:00

  • Production of recombinant porcine interferon alpha using PHB-intein-mediated protein purification strategy.

    abstract::Interferons (IFNs) are involved in the pathogenesis and recovery of viral and other infectious diseases. Recombinant IFNs have been used as anti-infectious agents exhibiting a broad range of antiviral and immunomodulatory properties in both human and domestic animals. In this report, we describe a highly efficient and...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-010-9101-2

    authors: Zhou X,Song Z,Liu X,Jia F,Wang Y

    更新日期:2011-04-01 00:00:00

  • Purification, characterization, and molecular cloning of a novel antifungal lectin from the roots of Ophioglossum pedunculosum.

    abstract::A novel mannan-specific lectin was isolated from the roots of a traditional Chinese herbal medicine, Ophioglossum pedunculosum through ion-exchange chromatography and gel filtration. With a molecular mass of 19,835.7 Da demonstrated by MALDI-TOF analysis, this novel agglutinin was designated as O. pedunculosum aggluti...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-011-9367-z

    authors: He XM,Ji N,Xiang XC,Luo P,Bao JK

    更新日期:2011-12-01 00:00:00

  • Study on mass transfer of isopropylbenzene and oxygen in a two-phase partitioning bioreactor in the presence of silicone oil.

    abstract::A two-phase partitioning bioreactor to treat gas effluents polluted by volatile organic compound has been developed. In this work, both the mass transfer of isopropylbenzene (IPB) and oxygen have been considered in relation to their influence on the hydrodynamics of the reactor and the type of silicone oils used as a ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-008-8480-0

    authors: Aldric JM,Lecomte JP,Thonart P

    更新日期:2009-05-01 00:00:00

  • A simple method for quantifying activity and survival of microorganisms involved in bioremediation processes.

    abstract::We have developed a substrate-induced growth response (SIGR) method for quantifying activity and population dynamics of microorganisms involved in bioremediation processes in soil and bioreactors. The biomass of organisms that can mineralize a given chemical can be estimated based on the concentration of that chemical...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02787924

    authors: Schmidt SK,Colores GM,Hess TF,Radehaus PM

    更新日期:1995-07-01 00:00:00

  • Enhanced hydrolysis and acidification of waste activated sludge by biosurfactant rhamnolipid.

    abstract::The effect of biosurfactant rhamnolipid (RL) on hydrolysis and acidification of waste activated sludge (WAS) was investigated. The results indicated that RL could greatly reduce the surface tension of sludge, resulting in stimulating the hydrolysis rate of WAS and enhancing the production of short-chain fatty acids (S...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0439-0

    authors: Yi X,Luo K,Yang Q,Li XM,Deng WG,Cheng HB,Wang ZL,Zeng GM

    更新日期:2013-11-01 00:00:00

  • Mesenchymal Stem Cells: a Promising Therapeutic Tool for Acute Kidney Injury.

    abstract::Acute kidney injury (AKI) is a rapid loss of renal function. It has high mortality rates. Still, renal replacement therapy is considered the best solution for recovering AKI. This opens a line of thought to develop an alternative therapy for it without complications. Mesenchymal stem cells are considered a new therapy...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-019-02995-2

    authors: Selim RE,Ahmed HH,Abd-Allah SH,Sabry GM,Hassan RE,Khalil WKB,Abouhashem NS

    更新日期:2019-09-01 00:00:00

  • Biodegradable polycaprolactone (PCL) nanosphere encapsulating superoxide dismutase and catalase enzymes.

    abstract::Biodegradable polycaprolactone (PCL) nanosphere encapsulating superoxide dismutase (SOD) and catalase (CAT) were successfully synthesized using double emulsion (w/o/w) solvent evaporation technique. Characterization of the nanosphere using dynamic light scattering, field emission scanning electron microscope, and Four...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0427-4

    authors: Singh S,Singh AN,Verma A,Dubey VK

    更新日期:2013-12-01 00:00:00

  • Anticancer and DNA binding activities of platinum (IV) complexes; importance of leaving group departure rate.

    abstract::The two six-coordinate Pt(IV) complexes, containing bidentate nitrogen donor/methyl ligands with general formula [Pt(X)2Me2((t)bu2bpy)], where (t)bu2bpy = 4,4'-ditert-butyl-2,2'-bipyridine and X = Cl (C1) or Br (C2), serving as the leaving groups were synthesized for evaluation of their anticancer activities and DNA b...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0700-6

    authors: Pouryasin Z,Yousefi R,Nabavizadeh SM,Rashidi M,Hamidizadeh P,Alavianmehr MM,Moosavi-Movahedi AA

    更新日期:2014-03-01 00:00:00

  • Controlled production of exopolysaccharides from Enterobacter A47 as a function of carbon source with demonstration of their film and emulsifying abilities.

    abstract::The bacterium Enterobacter A47 has demonstrated the ability to synthesise distinct exopolysaccharides (EPS) as a function of the substrate used. The culture's performance was evaluated in experiments using either glucose or xylose, as single carbon sources, and compared with the substrate (glycerol) used in previous s...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0560-0

    authors: Freitas F,Alves VD,Gouveia AR,Pinheiro C,Torres CA,Grandfils C,Reis MA

    更新日期:2014-01-01 00:00:00

  • Heterologous Expression of Aldehyde Dehydrogenase in Lactococcus lactis for Acetaldehyde Detoxification at Low pH.

    abstract::Aldehyde dehydrogenase (E.C. 1.2.1.x) can catalyze detoxification of acetaldehydes. A novel acetaldehyde dehydrogenase (istALDH) from the non-Saccharomyces yeast Issatchenkia terricola strain XJ-2 has been previously characterized. In this work, Lactococcus lactis with the NIsin Controlled Expression (NICE) System was...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-017-2573-6

    authors: Lyu Y,LaPointe G,Zhong L,Lu J,Zhang C,Lu Z

    更新日期:2018-02-01 00:00:00

  • Role of Met93 and Thr96 in the lid hinge region of Rhizopus chinensis lipase.

    abstract::We engineered Rhizopus chinensis lipase to study its critical amino acid role in catalytic properties. Based on the amino acid sequence and three-dimensional model of the lipase, residues located in its lid hinge region (Met93 and Thr96) were replaced with corresponding amino acid residues (Ile93 and Asn96) found in t...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0209-z

    authors: Zhu SS,Li M,Yu X,Xu Y

    更新日期:2013-05-01 00:00:00

  • Two-dimensional analysis of proteins specific to the bacterial magnetic particle membrane from Magnetospirillum sp. AMB-1.

    abstract::We report the identification of five proteins expressed specifically on the bacterial magnetic particle (BMP) membrane of Magnetospirillum sp. AMB-1. These proteins are major components of the BMP membrane. The molecular weights were determined to be 12.0, 16.0, 24.8, 35.6, and 66.2 kDa by sodium dodecyl sulfate polya...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:84-86:1-9:441

    authors: Okamura Y,Takeyama H,Matsunaga T

    更新日期:2000-04-01 00:00:00

  • Cytotoxic Tolerance of Healthy and Cancerous Bone Cells to Anti-microbial Phenolic Compounds Depend on Culture Conditions.

    abstract::Carnosol and carnosic acid are polyphenolic compounds found in rosemary and sage with known anti-oxidant, anti-inflammatory, and anti-microbial properties. Here, we addressed the potential use of carnosol and carnosic acid for in vitro bone tissue engineering applications, specifically depending on their cytotoxic eff...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-018-02934-7

    authors: Karadas O,Mese G,Ozcivici E

    更新日期:2019-06-01 00:00:00

  • Potential of Ca2+ in Mycobacterium tuberculosis H37Rv Pathogenesis and Survival.

    abstract::The host-pathogen interaction and involvement of calcium (Ca2+) signaling in tuberculosis infection is crucial and plays a significant role in pathogenesis. Ca2+ is known as a ubiquitous second messenger that could control multiple processes and is included in cellular activities like division, motility, stress respon...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s12010-016-2247-9

    authors: Sharma S,Meena LS

    更新日期:2017-02-01 00:00:00

  • Simulating the degradation of odor precursors in primary and waste-activated sludge during anaerobic digestion.

    abstract::Degradation of known odor precursors in sludge during anaerobic digestion was systematically studied and simulated using the Anaerobic Digestion Model Number 1 (ADM1). The degradation of various protein fractions (particulate, soluble, and bound), volatile fatty acids (VFAs), lipids, and amino acids of primary sludge ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-011-9213-3

    authors: Aldin S,Tu F,Nakhla G,Ray MB

    更新日期:2011-08-01 00:00:00