Purification, characterization, and molecular cloning of a novel antifungal lectin from the roots of Ophioglossum pedunculosum.

abstract：:An automated flow injection analysis (FIA) system for quantifying ethanol was developed using alcohol oxidase, horseradish peroxidase, 4-amino-phenazone, and phenol. A colorimetric detection method was developed using two different methods of analysis, with free and immobilized enzymes. The system with free enzymes pe...

journal_title：Applied biochemistry and biotechnology

authors： Alhadeff EM,Salgado AM,Pereira N Jr,Valdman B

更新日期：2004-04-01 00:00:00

abstract：:In this article, we describe a solid-state photoelectrochemical cell for light detection. Safranine-T dye mixed with polyvinyl alcohol (PVA) solution was deposited on a conducting and transparent indium-tin-oxide (ITO)-coated glass that was used as one electrode and another ITO-coated glass as the counterelectrode. A ...

journal_title：Applied biochemistry and biotechnology

authors： Dey SK,Manik NB

更新日期：2001-10-01 00:00:00

abstract：:The first continuous production system of laminaribiose from sucrose and glucose in a bienzymatic reaction is reported in this study. Immobilized laminaribiose phosphorylase and sucrose phosphorylase were used in a packed bed reactor system comprising of a 3-cm glass column at 35 °C with a steady feeding flow rate of ...

journal_title：Applied biochemistry and biotechnology

authors： Abi A,Wang A,Jördening HJ

更新日期：2018-12-01 00:00:00

abstract：:Lipase from Rhizomucor miehei (RML) was immobilized onto chitosan support in the presence of some surfactants added at low levels using two different strategies. In the first approach, the enzyme was immobilized in the presence of surfactants on chitosan supports previously functionalized with glutaraldehyde. In the s...

journal_title：Applied biochemistry and biotechnology

authors： de Oliveira UMF,Lima de Matos LJB,de Souza MCM,Pinheiro BB,Dos Santos JCS,Gonçalves LRB

更新日期：2018-04-01 00:00:00

abstract：:A new cellulase producer strain of Penicillium digitatum (RV 06) was previously obtained from rotten maize grains. This work aim was to optimize the production and characterize this microorganism produced cellulase. A CMCase maximum production (1.6 U/mL) was obtained in stationary liquid culture, with an initial pH of...

journal_title：Applied biochemistry and biotechnology

authors： Dos Santos FC,de Oliveira MAS,Seixas FAV,Barbosa-Tessmann IP

更新日期：2020-09-01 00:00:00

abstract：:A genetically engineered Escherichia coli cell expressing both organophosphorus hydrolase (OPH) and carboxyl esterase (CaE) B1 intracellularly was constructed and cultivated. The harvested wet cells were vacuum dried, and the storage stability of the dried cell powder was determined in terms of OPH activity. Over a pe...

journal_title：Applied biochemistry and biotechnology

authors： Zheng YZ,Lan WS,Qiao CL,Mulchandani A,Chen W

更新日期：2007-03-01 00:00:00

abstract：:IOGEN Corporation of Ottawa, Canada, has recently built a 40t/d biomass-to-ethanol demonstration plant adjacent to its enzyme production facility. It has partnered with the University of Toronto to test the C6/C5 cofermenta-tion performance characteristics of the National Renewable Energy Labora-tory's metabolically e...

journal_title：Applied biochemistry and biotechnology

authors： Lawford HG,Rousseau JD

更新日期：2002-04-01 00:00:00

abstract：:Polygalacturonases are part of the group of enzymes involved in pectin degradation. The aim of this work was to investigate some of the factors affecting polygalacturonase production by an Aspergillus giganteus strain and to characterize this pectinolytic activity. Several carbon sources, both pure substances and natu...

journal_title：Applied biochemistry and biotechnology

authors： Pedrolli DB,Gomes E,Monti R,Carmona EC

更新日期：2008-02-01 00:00:00

abstract：:Thermomucor indicae-seudaticae, a glucoamylase-producing thermophilic mould, was mutagenised using nitrous acid and gamma ((60)Co) irradiation in a sequential manner to isolate deregulated mutants for enhanced production of glucoamylase. The mutants were isolated on Emerson YpSs agar containing a non-metabolisable glu...

journal_title：Applied biochemistry and biotechnology

authors： Kumar P,Satyanarayana T

更新日期：2009-07-01 00:00:00

abstract：:Anthracnose is a major disease in Florida hybrid bunch grapes, caused by a fungus viz. Elsinoe ampelina. Florida hybrid bunch grapes are grown in southeastern USA for their superior wine characteristics. However, the effect of anthracnose on grape productivity and wine quality is a major concern to grape growers. Our ...

journal_title：Applied biochemistry and biotechnology

authors： Vasanthaiah HK,Katam R,Basha SM

更新日期：2009-06-01 00:00:00

abstract：:Sorbitol, a polyol found in many fruits, is attracting increasing industrial interest as a sweetener, humectant, texturizer, and softener. It is principally produced by chemical means. The bacterium Zymomonas mobilis is able to produce sorbitol together with gluconic acid from fructose and glucose, respectively. This ...

journal_title：Applied biochemistry and biotechnology

authors： Jonas R,Silveira MM

更新日期：2004-07-01 00:00:00

abstract：:The main goal of this paper was to elaborate the possibility of industrial application of biosorption properties of Enteromorpha prolifera (production of mineral feed additives for livestock). In this study, biosorption process was used in the binding of chromium(III) ions from aqueous solution by the green macroalga....

journal_title：Applied biochemistry and biotechnology

authors： Michalak I,Chojnacka K

更新日期：2010-03-01 00:00:00

abstract：:Soybean hulls were subjected to thermo-mechanical extrusion pretreatment at various in-barrel moisture contents and screw speeds. Extrusion degraded the lignocellulosic structure and enhanced enzymatic hydrolysis of soybean hulls, with up to 155% increase in glucose yield as compared to untreated substrate. Greater gl...

journal_title：Applied biochemistry and biotechnology

authors： Yoo J,Alavi S,Vadlani P,Behnke KC

更新日期：2012-02-01 00:00:00

abstract：:For efficiently enhancing the activity of isoeugenol monooxygenase, a whole cell overproducing active aggregate IEM720-18A was successfully fabricated via the fusion of amphiphilic short peptide 18A (EWLKAFYEKVLEKLKELF) and isoeugenol monooxygenase and then efficiently expressed in E. coli BL21 (DE3). Such resulting b...

journal_title：Applied biochemistry and biotechnology

authors： Zhao L,Jiang Y,Fang H,Zhang H,Cheng S,Rajoka MSR,Wu Y

更新日期：2019-10-01 00:00:00

abstract：:The increase in waste disposal and energy costs has provided an incentive to convert carbohydrate-rich food waste streams into fuel. For example, dining halls and restaurants discard foods that require tipping fees for removal. An effective use of food waste may be the enzymatic hydrolysis of the waste to simple sugar...

journal_title：Applied biochemistry and biotechnology

authors： Davis RA

更新日期：2008-03-01 00:00:00

abstract：:A 28-day feeding experiment with formulated feed using docosahexaenoic acid (DHA)-rich whole cells of freeze-dried marine microalgae Schizochytrium sp. to understand the distribution of fatty acids in a laboratory model zebrafish was conducted. Three feeds, commercial feed, 50:50 feed (50% commercial and 50% algae), a...

journal_title：Applied biochemistry and biotechnology

authors： Byreddy AR,Yoganantharjah P,Gupta A,Gibert Y,Puri M

更新日期：2019-05-01 00:00:00

abstract：:Waste frying oil, which not only harms people's health but also causes environmental pollution, can be a good alternative to partially substitute petroleum diesel through transesterification reaction. This oil contained 8.8 % of free fatty acids, which cause a problem in a base-catalyzed process. In this study, synthe...

journal_title：Applied biochemistry and biotechnology

authors： Romdhane IB,Romdhane ZB,Bouzid M,Gargouri A,Belghith H

更新日期：2013-12-01 00:00:00

abstract：:The first step involved in the degradation of phthalate isomers (phthalate, isophthalate and terephthalate) is the double hydroxylation by respective aromatic-ring hydroxylating dioxygenases. These are two component enzymes consisting of 'oxygenase' and 'reductase' components. Soil isolate Pseudomonas aeruginosa strai...

journal_title：Applied biochemistry and biotechnology

authors： Karandikar R,Badri A,Phale PS

更新日期：2015-09-01 00:00:00

abstract：:Bacterial strain NYT501, which we previously isolated from soil, was identified as Stenotrophomonas maltophilia, and it was confirmed that this strain produces an intracellular β-N-acetylhexosaminidase exhibiting transglycosylation activity. Several properties of this enzyme were characterized using a partially purifi...

journal_title：Applied biochemistry and biotechnology

authors： Uehara A,Takahashi N,Moriyama M,Hirano T,Hakamata W,Nishio T

更新日期：2018-02-01 00:00:00

abstract：:The adaptive response of the fungus Blakeslea trispora to the oxidative stress induced by butylated hydroxytoluene (BHT) during carotene production in shake flask culture was investigated. The culture response to oxidative stress was studied by measuring the specific activities of catalase (CAT) and superoxide dismuta...

journal_title：Applied biochemistry and biotechnology

authors： Nanou K,Roukas T

更新日期：2010-04-01 00:00:00

abstract：:The inhibitory effects of pH and acetic acid on the co-fermentation of glucose and xylose in complex medium by recombinant flocculent Saccharomyces cerevisiae MA-R4 were evaluated. In the absence of acetic acid, the fermentation performance of strain MA-R4 was similar between pH 4.0-6.0, but was negatively affected at...

journal_title：Applied biochemistry and biotechnology

authors： Matsushika A,Sawayama S

更新日期：2012-12-01 00:00:00

abstract：:Entrapped cells of P. diminuta were used for the production of 7-aminocephalosporanic acid (7-ACA), a key intermediate required for the production of most of the clinically used cephalosporin derivatives, i.e., semisynthetic cephalosporins. The repeated batch production of 7-ACA with entrapped cells of P. diminuta in ...

journal_title：Applied biochemistry and biotechnology

authors： Nigam VK,Kundu S,Ghosh P

更新日期：2007-04-01 00:00:00

abstract：:Dissolved oxygen (DO) is an imperative parameter of the activated sludge process (ASP) for wastewater bioremediation. The effect of DO on microbial communities and corresponding metabolic functions in wastewater bioremediation was investigated using next-generation analysis techniques in this study. Illumina-based who...

journal_title：Applied biochemistry and biotechnology

authors： Yadav TC,Jadeja NB,Kapley A

更新日期：2020-10-01 00:00:00

abstract：:Large-volume parenteral solutions were submitted to heat treatments after being inoculated with Bacillus stearothermophilus ATCC 7953 (Tr = 121 degrees C) and Bacillus subtilis ATCC 9372 (Tr = 104.5 degrees C) spores. The average decimal reduction time for B. stearothermophilus ranged from a D121 degrees C value of 1....

journal_title：Applied biochemistry and biotechnology

authors： Penna TC,Marques M,Machoshvili IA,Ishii M

更新日期：2002-04-01 00:00:00

abstract：:Hydrolysates were obtained from dilute sulfuric acid pretreatment of whole-tree softwood forest thinnings and softwood sawdust. Mid-infrared (IR) spectra were obtained on sample sets of wet washed hydrolysates, and 45 degrees C vacuum-dried washed hydrolysates, using a Fourier transform infrared (FTIR) spectrophotomet...

journal_title：Applied biochemistry and biotechnology

authors： Tucker MP,Nguyen QA,Eddy FP,Kadam KL,Gedvilas LM,Webb JD

更新日期：2001-04-01 00:00:00

abstract：:The purpose of this study was to investigate the feasibility of immobilizing Yarrowia lipolytica lipase lip2 on epoxy microspheres with or without gelatin modifications. The activity of lipase immobilized on gelatin-modified supports was twofold higher than those immobilized on native supports. There was no significan...

journal_title：Applied biochemistry and biotechnology

authors： Xie R,Cui C,Chen B,Tan T

更新日期：2015-10-01 00:00:00

abstract：:The aim of this work was to establish optimal conditions for the maximum production of endo-beta-1,4 mannanases using cheaper sources. Eight thermotolerant fungal strains were isolated from garden soil and compost samples collected in and around the Gulbarga University campus, India. Two strains were selected based on...

journal_title：Applied biochemistry and biotechnology

authors： Kote NV,Patil AG,Mulimani VH

更新日期：2009-02-01 00:00:00

abstract：:6-Tuliposides A (6-PosA) and B (6-PosB) are major secondary metabolites in tulip (Tulipa gesneriana), having an acyl group at the C-6 position of D-glucose. They serve as precursors of the antimicrobial α-methylene-γ-butyrolactones tulipalins A (PaA) and B (PaB). The conversions of 6-PosA/6-PosB to PaA/PaB are catalyz...

journal_title：Applied biochemistry and biotechnology

authors： Nomura T,Ogita S,Kato Y

更新日期：2019-05-01 00:00:00

abstract：:meso-2,3-Butanediol (meso-2,3-BDO) is essential for the synthesis of various economically valuable biosynthetic products; however, the production of meso-2,3-BDO from expensive carbon sources is an obstacle for industrial applications. In this study, genes involved in the synthesis of 2,3-BDO in Klebsiella pneumoniae ...

journal_title：Applied biochemistry and biotechnology

authors： Lee S,Kim B,Park K,Um Y,Lee J

更新日期：2012-04-01 00:00:00

abstract：:Several recombinant derivatives of serine protease inhibitor called silk protease inhibitor 2 (SPI2), which is a silk component in Galleria mellonella (Lepidoptera, Insecta), were prepared in the expression vector Pichia pastoris. Both the native and the recombinant protease inhibitors were highly active against subti...

journal_title：Applied biochemistry and biotechnology

authors： Kodrík D,Kludkiewicz B,Navrátil O,Skoková Habuštová O,Horáčková V,Svobodová Z,Vinokurov KS,Sehnal F

更新日期：2013-09-01 00:00:00