Studies on productivity and characterization of polygalacturonase from Aspergillus giganteus submerged culture using citrus pectin and orange waste.

abstract：:A soluble glucoside 3-dehydrogenase (G3DH) was purified from a newly isolated Sphingobacterium faecium ZJF-D6 CCTCC M 2013251. The enzyme was purified to 35.71-fold with a yield of 41.91 % and was estimated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis with a molecular mass of 62 kDa. The sequences of ...

journal_title：Applied biochemistry and biotechnology

authors： Zhang JF,Yang B,Chen W,Chen J

更新日期：2014-04-01 00:00:00

abstract：:In rapidly growing developing countries, waste disposal is a major challenge. Current waste disposal methods (e.g., landfills and sewage treatment) incur costs and often are not employed; thus, wastes accumulate in the environment. To address this challenge, it is advantageous to create economic incentives to collect ...

journal_title：Applied biochemistry and biotechnology

authors： Lonkar S,Fu Z,Wales M,Holtzapple M

更新日期：2017-01-01 00:00:00

abstract：:The effect of organic matter and fill time on anaerobic sequencing batch reactor (5 L, 30 °C, 8-h cycles, 50 rpm) efficiency has been analyzed. Organic matter was increased by the influent concentration. Fill times investigated were in the batch mode and fed-batch followed by batch. In the batch mode organic matter re...

journal_title：Applied biochemistry and biotechnology

authors： Selma VC,Cotrim LH,Rodrigues JA,Ratusznei SM,Zaiat M,Foresti E

更新日期：2010-12-01 00:00:00

abstract：:Dual-responsive poly(N,N-diethylacrylamide) (PDEA) hydrogel films with entrapped horseradish peroxidase (HRP) and glucose oxidase (GOD) were successfully prepared on electrode surface with a simple one-step polymerization procedure under mild conditions, designated as PDEA-HRP-GOD. Cyclic voltammetric (CV) response of...

journal_title：Applied biochemistry and biotechnology

authors： Yao H,Lin L,Wang P,Liu H

更新日期：2014-08-01 00:00:00

abstract：:In this work, we engineered the α/β fold of mannanase Man23 based on its molecular structure analysis to obtain more stable variants. By introducing 31 single-site mutations in the α/β fold and shuffling them, the incorporation of four mutations (K178R, K207R, N340R, and S354R) displayed a good balance between high ac...

journal_title：Applied biochemistry and biotechnology

authors： Zhou HY,Pan HY,Rao LQ,Wu YY

更新日期：2011-01-01 00:00:00

abstract：:In this study, we investigated structural changes in alpha-glucosidase during urea denaturation. Alpha-glucosidase was inactivated by urea in a dose-dependent manner. The inactivation was a first-order reaction with a monophase process. Urea inhibited alpha-glucosidase in a mixed-type reaction. We found that an increa...

journal_title：Applied biochemistry and biotechnology

authors： Wu XQ,Wang J,Lü ZR,Tang HM,Park D,Oh SH,Bhak J,Shi L,Park YD,Zou F

更新日期：2010-03-01 00:00:00

abstract：:Lignin peroxidase was purified (72-fold) from Acinetobacter calcoaceticus NCIM 2890. The purified lignin peroxidase (55-65 kDa) showed dimeric nature. The maximum enzyme activity was observed at pH 1.0, between a broad temperature range of 50 and 70 degrees C, at H2O2 concentration (40 mM) and the substrate concentrat...

journal_title：Applied biochemistry and biotechnology

authors： Ghodake GS,Kalme SD,Jadhav JP,Govindwar SP

更新日期：2009-01-01 00:00:00

abstract：:The main goal of this paper was to elaborate the possibility of industrial application of biosorption properties of Enteromorpha prolifera (production of mineral feed additives for livestock). In this study, biosorption process was used in the binding of chromium(III) ions from aqueous solution by the green macroalga....

journal_title：Applied biochemistry and biotechnology

authors： Michalak I,Chojnacka K

更新日期：2010-03-01 00:00:00

abstract：:The use of stalks instead of tubers as a source of carbohydrates for ethanol production has been investigated. The inulin present in the stalks of Jerusalem artichoke was extracted with water and the effect of solid-liquid ratio, temperature, and acid addition was studied and optimized in order to attain a high-fructo...

journal_title：Applied biochemistry and biotechnology

authors： Negro MJ,Ballesteros I,Manzanares P,Oliva JM,Sáez F,Ballesteros M

更新日期：2006-04-01 00:00:00

abstract：:A green synthesis of iron oxide nanoparticles (FeNPs) was developed using Murraya koenigii leaf extract as reducing and stabilizing agent. UV-vis spectra show that the absorption band centred at a wavelength of 277 nm which corresponds to the surface plasmon resonances of synthesized FeNPs. Fourier transform infrared ...

journal_title：Applied biochemistry and biotechnology

authors： Mohanraj S,Kodhaiyolii S,Rengasamy M,Pugalenthi V

更新日期：2014-05-01 00:00:00

abstract：:The cellulolytic fungus, Trichoderma has oval and mononucleate conidia. When these conidia are incubated in a liquid medium, they begin to swell and their shape becomes spherical followed by an increase in inner space. In such swollen conidia, it is possible to produce a larger autopolyploid nucleus using a mitotic ar...

journal_title：Applied biochemistry and biotechnology

authors： Toyama H,Yano M,Hotta T

更新日期：2004-04-01 00:00:00

abstract：:Soaking in aqueous ammonia (SAA) pretreatment was investigated to improve enzymatic digestibility and consequently to increase total fermentable sugar production from barley straw. Various effects of pretreatment process parameters, such as reaction temperature, reaction time, solid:liquid ratio, and ammonia concentra...

journal_title：Applied biochemistry and biotechnology

authors： Yoo CG,Nghiem NP,Hicks KB,Kim TH

更新日期：2013-04-01 00:00:00

abstract：:The abilities of lignin peroxidase (LIP) and manganese peroxidase (MNP) from Phanerochaete chrysosporium to degrade an insoluble hardwood lignin in vitro in aqueous media were tested. Neither LIP nor MNP appreciably changed the mass or lignin content, although both produced small amounts of unique solubilized lignin f...

journal_title：Applied biochemistry and biotechnology

authors： Thompson DN,Hames BR,Reddy CA,Grethlein HE

更新日期：1998-04-01 00:00:00

abstract：:Phytochelatin synthase (PCS) is a key enzyme involved in the synthesis of phytochelatins, which are thought to play important roles in heavy metal detoxification. The sacred lotus (Nelumbo nucifera), one of the most popular ornamental species, has been shown to be a potential phytoremediator of heavy metal polluted wa...

journal_title：Applied biochemistry and biotechnology

authors： Liu Z,Gu C,Chen F,Yang D,Wu K,Chen S,Jiang J,Zhang Z

更新日期：2012-02-01 00:00:00

abstract：:Crosslinked enzyme aggregates (CLEAs) of a partially purified penicillin acylase from a recombinant Escherichia coli strain have been produced as a novel type of biocatalysts well endowed to perform in organic media. Different protein precipitants were studied and glutaraldehyde was used as the crosslinking agent. Pre...

journal_title：Applied biochemistry and biotechnology

authors： Illanes A,Wilson L,Caballero E,Fernández-Lafuente R,Guisán JM

更新日期：2006-06-01 00:00:00

abstract：:Large-scale amine-based CO(2) capture will generate waste containing large amounts of ammonia, in addition to contaminants such as the actual amine as well as degradation products thereof. Monoethanolamine (MEA) has been a dominant amine applied so far in this context. This study reveals how biological N removal can b...

journal_title：Applied biochemistry and biotechnology

authors： Hauser I,Colaço AB,Skjæran JA,Einbu A,Ostgaard K,Svendsen HF,Cervantes FJ

更新日期：2013-02-01 00:00:00

abstract：:The effect of different concentrations of zinc on the bioaccumulation of zinc and biomass yield in both mycelium and fruiting body of Pleurotus florida cultivated in liquid medium was studied. The results showed that the optimum yield of mycelia (11.33 ± 0.44 g/L) and fruiting bodies (7.70 ± 0.19 g/L) dry biomass was ...

journal_title：Applied biochemistry and biotechnology

authors： Poursaeid N,Azadbakht A,Balali GR

更新日期：2015-04-01 00:00:00

abstract：:The gate surface of an ion-sensitive field effect transistor (ISFET) was modified with Langmuir-Blodgett (LB) film composed of fatty acid or crown ether amphiphiles to examine their potentiometric response to H+ and K+ ions. The results demonstrate the possible use of the lipid films for preparing ISFET ion sensors. ...

journal_title：Applied biochemistry and biotechnology

authors： Osa T

更新日期：1993-04-01 00:00:00

abstract：:Poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) [poly(HEMA-GMA)] cryogel was synthesized by cryopolymerization technique at frozen temperature. Iminodiacetic acid (IDA) was then attached covalently to the cryogel as a chelating agent. Then, poly(HEMA-GMA)-IDA cryogel was chelated with Ni(II) ions and this n...

journal_title：Applied biochemistry and biotechnology

authors： Uygun M,Akduman B,Akgöl S,Denizli A

更新日期：2013-08-01 00:00:00

abstract：:Ovarian malignancy is diagnosed in nearly a fourth of a million women internationally every year. Methylation of RASSF1A tumor suppressor gene prompts its inactivation in diseases. In this study, the RASSF1A promoter methylation was detected by methylated-specific PCR and investigated serum RASSF1A protein level throu...

journal_title：Applied biochemistry and biotechnology

authors： Rezk NA,Mohamed RH,Alnemr AA,Harira M

更新日期：2018-05-01 00:00:00

abstract：:S-Adenosylhomocysteine hydrolase (SAHase) encoded by sahase gene is a determinant when catalyzing the reversible conversion of adenosine and homocysteine to S-adenosylhomocysteine in most living organisms. The sahase gene was isolated from the genome of the highly thermostable anaerobic bacteria Thermotoga maritima, a...

journal_title：Applied biochemistry and biotechnology

authors： Lozada-Ramírez JD,Sánchez-Ferrer A,García-Carmona F

更新日期：2013-06-01 00:00:00

abstract：:Adenosine monophosphate-activated protein kinase (AMPK) is viewed as a privileged therapeutic target for several diseases such as cancer, diabetes, inflammation, obesity, etc. In addition, AMPK has entered the limelight of current drug discovery with its evolution as a key metabolic regulator. AMPK also plays a key ro...

journal_title：Applied biochemistry and biotechnology

authors： Ramesh M,Vepuri SB,Oosthuizen F,Soliman ME

更新日期：2016-02-01 00:00:00

abstract：:A 28-day feeding experiment with formulated feed using docosahexaenoic acid (DHA)-rich whole cells of freeze-dried marine microalgae Schizochytrium sp. to understand the distribution of fatty acids in a laboratory model zebrafish was conducted. Three feeds, commercial feed, 50:50 feed (50% commercial and 50% algae), a...

journal_title：Applied biochemistry and biotechnology

authors： Byreddy AR,Yoganantharjah P,Gupta A,Gibert Y,Puri M

更新日期：2019-05-01 00:00:00

abstract：:The pH dependencies of electrochemomechanical deformation (ECMD) including the cyclic voltammetry and the expansion ratio in conducting polymers, polyaniline (PANI), and poly(o-methoxyaniline) film were studied to elucidate the mechanisms. It was found that the ECMD is governed by the conformational change of polymer ...

journal_title：Applied biochemistry and biotechnology

authors： Kaneto K,Kaneko M

更新日期：2001-10-01 00:00:00

abstract：:This study represents the development of a new batch method by consortium GG-BL using two microbial cultures viz., Galactomyces geotrichum MTCC 1360 and Brevibacillus laterosporus MTCC 2298, by varying environmental conditions for the decolorization and biodegradation of Rubine GFL. Consortium was found to give better...

journal_title：Applied biochemistry and biotechnology

authors： Waghmode TR,Kurade MB,Lade HS,Govindwar SP

更新日期：2012-07-01 00:00:00

abstract：:IOGEN Corporation of Ottawa, Canada, has recently built a 40t/d biomass-to-ethanol demonstration plant adjacent to its enzyme production facility. It has partnered with the University of Toronto to test the C6/C5 cofermenta-tion performance characteristics of the National Renewable Energy Labora-tory's metabolically e...

journal_title：Applied biochemistry and biotechnology

authors： Lawford HG,Rousseau JD

更新日期：2002-04-01 00:00:00

abstract：:A new medical application of an immobilized microbial enzyme is described. Extracorporeal devices require systemic heparin administration to prevent thrombus formation; however, the use of heparin often leads to serious hemorrhagic complications. Heparinase isolated from Flavobacterium has been immobilized and used in...

journal_title：Applied biochemistry and biotechnology

authors： Linhardt RJ,Cooney CL,Tapper D,Zannetos CA,Larsen AK,Langer R

更新日期：1984-02-01 00:00:00

abstract：:In this study, an easily detectable method was employed for screening laccase-producing microorganisms by using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) as laccase secretion indicator. A novel laccase-producing strain was isolated and identified as Shiraia bambusicola Henn. strain GZ11K2 according to the mor...

journal_title：Applied biochemistry and biotechnology

authors： Du W,Sun C,Yu J,Liang J,Liang Z,Han Y,Zou X

更新日期：2012-12-01 00:00:00

abstract：:Significant components of cost-effective medium for Magnusiomyces capitatus A4C extracellular lipase (ECL) production were optimized via a five-level factorial design. A simplistic, economical, and green approach was adopted for biomimetic mineralization to prepare multilayered nano-entrapped ECL, which were then appl...

journal_title：Applied biochemistry and biotechnology

authors： Baloch KA,Upaichit A,Cheirsilp B

更新日期：2021-01-01 00:00:00

abstract：:This investigation utilized sugarcane bagasse aqueous extract (SBAE), a nontoxic, cost-effective medium to boost triacylglycerol (TAG) accumulation in novel fresh water microalgal isolate Scenedesmus sp. IITRIND2. Maximum lipid productivity of 112 ± 5.2 mg/L/day was recorded in microalgae grown in SBAE compared to mod...

journal_title：Applied biochemistry and biotechnology

authors： Arora N,Patel A,Pruthi PA,Pruthi V

更新日期：2016-09-01 00:00:00