Crosslinked penicillin acylase aggregates for synthesis of beta-lactam antibiotics in organic medium.


:Crosslinked enzyme aggregates (CLEAs) of a partially purified penicillin acylase from a recombinant Escherichia coli strain have been produced as a novel type of biocatalysts well endowed to perform in organic media. Different protein precipitants were studied and glutaraldehyde was used as the crosslinking agent. Precipitation curves were obtained for all precipitants to determine the concentrations at which all the protein precipitated out of the solution. The effect of the glutaraldehyde-to-protein ratio was studied with respect to process recovery and the specific activity and stability of the biocatalyst. Recovery of penicillin acylase activity was moderately high, about 50%; major losses of enzyme activity were produced at the precipitation step. Specific activities of all CLEAs were very high, which is one of the advantages of using nonsupported biocatalysts. Ammonium sulfate and tert-butyl alcohol were the best precipitants at a glutaraldehyde-protein mass ratio of 2 and were selected to perform the kinetically controlled synthesis of ampicillin in 60% (v/v) ethylene glycol medium. At comparable conversion yields, volumetric and specific antibiotic productivity were much higher for CLEAs than for carrier-bound penicillin acylases.


Illanes A,Wilson L,Caballero E,Fernández-Lafuente R,Guisán JM




Has Abstract


2006-06-01 00:00:00














  • Antioxidative Metabolites Synthesized by Marine Pigmented Vibrio sp. and Its Protection on Oxidative Deterioration of Membrane Lipids.

    abstract::Bacterial strain Vibrio sp. (PIGB 184) isolated from water samples of the Arabian Sea and identified through 16S rRNA demonstrated the production of pigmentary antioxidants with higher ABTS activities 90.9 ± 0.42 % in comparison with the standard commercial pigmented antioxidant, quercetin 88.8 ± 1.4 %. Antioxidative ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Pawar R,Mohandass C,Dastager SG,Kolekar YM,Malwankar R

    更新日期:2016-04-01 00:00:00

  • Antibacterial activity of Alpinia galanga (L) Willd crude extracts.

    abstract::Methanol, acetone and diethyl ether extracts of Alpinia galanga have been evaluated against pathogens viz. Bacillus subtilis MTCC 2391, Enterobacter aerogene, Enterobacter cloacae, Enterococcus faecalis, Escherichia coli MTCC 1563, Klebsiella pneumoniae, Pseudomonas aeruginosa MTCC 6642, Salmonella typhimurium, Staphy...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Rao K,Ch B,Narasu LM,Giri A

    更新日期:2010-10-01 00:00:00

  • Catalytic antibodies for complex reactions: hapten design and the importance of screening for catalysis in the generation of catalytic antibodies for the NDA/CN reaction.

    abstract::Success in generating catalytic antibodies as enzyme mimics lies in the strategic design of the transition-state analog (TSA) for the reaction of interest, and careful development of screening processes for the selection of antibodies that are catalysts. Typically, the choice of TSA structure is straightforward, and t...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: DeSilva BS,Orosz G,Egodage KL,Carlson RG,Schowen RL,Wilson GS

    更新日期:2000-01-01 00:00:00

  • MicroRNA-499a-5p Promotes Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells to Cardiomyocytes.

    abstract::Since the adult mammalian heart has limited regenerative capacity, cardiac trauma, disease, and aging cause permanent loss of contractile tissue. This has fueled the development of stem cell-based strategies to provide the damaged heart with new cardiomyocytes. Bone marrow-derived mesenchymal stem cells (BM-MSCs) are ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Neshati V,Mollazadeh S,Fazly Bazzaz BS,de Vries AAF,Mojarrad M,Naderi-Meshkin H,Neshati Z,Mirahmadi M,Kerachian MA

    更新日期:2018-09-01 00:00:00

  • Reusability of entrapped cells of Pseudomonas diminuta for production of 7-aminocephalosporanic acid.

    abstract::Entrapped cells of P. diminuta were used for the production of 7-aminocephalosporanic acid (7-ACA), a key intermediate required for the production of most of the clinically used cephalosporin derivatives, i.e., semisynthetic cephalosporins. The repeated batch production of 7-ACA with entrapped cells of P. diminuta in ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Nigam VK,Kundu S,Ghosh P

    更新日期:2007-04-01 00:00:00

  • Influence of temperature on performance of an anaerobic sequencing biofilm batch reactor with circulation applied to treatment of low-strength wastewater.

    abstract::The effect of temperature on the performance of an anaerobic sequencing biofilm batch reactor (ASBBR) with liquid-phase recirculation was assessed. Assays were performed using a recirculation velocity of 0.20 cm/s, 8-h cycles, and an average treated synthetic wastewater volume of 2 L/cycle with a concentration of 500 ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Agibert SA,Moreira MB,Ratusznei SM,Rodrigues JA,Zaiat M,Foresti E

    更新日期:2007-02-01 00:00:00

  • Structure Characterization and Lead Detoxification Effect of Carboxymethylated Melanin Derived from Lachnum Sp.

    abstract::In the present study, an intracellular melanin, named LIM205, was separated from Lachnum YM205 mycelia and was purified on a Sephadex G-15 column. The molecular weight of LIM205 was determined as 522 Da, and its molecular formula was speculated as C28H14N2O7S. The possible chemical structure of LIM205 was determined a...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Zong S,Li L,Li J,Shaikh F,Yang L,Ye M

    更新日期:2017-06-01 00:00:00

  • Characteristics of White-rot Fungus Phlebia brevispora TMIC33929 and Its Growth-Promoting Bacterium Enterobacter sp. TN3W-14 in the Decolorization of Dye-Contaminated Water.

    abstract::The dye decolorization potential of the white-rot fungus Phlebia brevispora TMIC33929 when grown alone or in coculture with its growth-promoting bacterium Enterobacter sp. TN3W-14 was evaluated in low nitrogen liquid medium at different pHs. Axenic fungus removed a similar amount of Congo red and crystal violet at pH ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Harry-Asobara JL,Kamei I

    更新日期:2019-12-01 00:00:00

  • Effects of organic loading, influent concentration, and feed time on biohydrogen production in a mechanically stirred AnSBBR treating sucrose-based wastewater.

    abstract::An anaerobic sequencing batch biofilm reactor (AnSBBR-total volume 7.5 L; liquid volume 3.6 L; treated volume per cycle 1.5 L) treated sucrose-based wastewater to produce biohydrogen (at 30 °C). Different applied volumetric organic loads (AVOL of 9.0, 12.0, 13.5, 18.0, and 27.0 kg COD m(-3) day(-1)), which were varied...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Manssouri M,Rodrigues JA,Ratusznei SM,Zaiat M

    更新日期:2013-12-01 00:00:00

  • Trehalose biosynthesis enhancement for six yeast strains under pressurized culture.

    abstract::Six yeast strains of the commercial brewing yeasts CICC1391 and CICC1471, the commercial baker yeasts CICC1339 and CICC1447, and the commercial alcohol yeasts CICC1286 and CICC1291 have been cultured under 1.0 MPa of pressure with N(2) and CO(2) as pressure media. The concentration of intracellular trehalose and the a...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Qiao C,Jia S,Dai Y,Wang R,Sun A

    更新日期:2010-01-01 00:00:00

  • Soybean hulls pretreated using thermo-mechanical extrusion--hydrolysis efficiency, fermentation inhibitors, and ethanol yield.

    abstract::Soybean hulls were subjected to thermo-mechanical extrusion pretreatment at various in-barrel moisture contents and screw speeds. Extrusion degraded the lignocellulosic structure and enhanced enzymatic hydrolysis of soybean hulls, with up to 155% increase in glucose yield as compared to untreated substrate. Greater gl...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Yoo J,Alavi S,Vadlani P,Behnke KC

    更新日期:2012-02-01 00:00:00

  • Co-aggregation of laccase and nature egg white: a simple method to prepare stable and recyclable biocatalyst.

    abstract::Cross-linked enzyme aggregates (CLEAs) are a versatile and effective method for enzyme immobilization, which is exquisitely simple and amenable to rapid optimization. In this study, nature egg white, which is low cost, easily available, and nontoxic, was used as protein feeder to replace traditional protein feeder (bo...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Jiang Y,Wang Q,He Y,Zhou L,Gao J

    更新日期:2014-03-01 00:00:00

  • Prediction of folding stability and degradability of the de novo designed protein MB-1 in cow rumen.

    abstract::The authors have recently reported on the design of a protein (MB-1) enriched in methionine, threonine, lysine, and leucine. The protein is intended to be produced by rumen bacteria, in a way that would provide high producing lactating cows with limiting amino acids. In this report, MB-1 stability in the rumen is asse...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: MacCallum JD,Hefford MA,Omar S,Beauregard M

    更新日期:1997-04-01 00:00:00

  • Polysaccharide hydrolase folds diversity of structure and convergence of function.

    abstract::Polysaccharide glycosyl hydrolases are a group of enzymes that hydrolyze the glycosidic bond between carbohydrates or between a carbohydrate and a noncarbohydrate moiety. Here we illustrate that traditional schemes for grouping enzymes, such as by substrate specificity or by organism of origin, are not appropriate whe...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Himmel ME,Karplus PA,Sakon J,Adney WS,Baker JO,Thomas SR

    更新日期:1997-04-01 00:00:00

  • Observation of geometric structure of collagen molecules by atomic force microscopy.

    abstract::Atomic force microscopy was used to study the geometric structure of collagen fibrils and molecules of rat calcanean tendon tissues. The authors found that the diameter of the fibrils ranged from 124 to 170 nm, and their geometric form suggested a helical winding with spectral period from 59.4 to 61.7 nm, close to the...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Baranauskas V,Vidal BC,Parizotto NA

    更新日期:1998-02-01 00:00:00

  • The Role of kguT Gene in 2-Ketogluconate-Producing Pseudomonas plecoglossicida JUIM01.

    abstract::Pseudomonas are ubiquitous bacteria that can live under a wide range of environmental conditions; some species are successfully used in the fermentation industry. Pseudomonas plecoglossicida JUIM01 strain is an important industrial 2-ketogluconate (2KGA) producer. In Pseudomonas, kguT gene encodes a putative 2KGA tran...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Sun W,Wang Q,Luan F,Man Z,Cui F,Qi X

    更新日期:2019-03-01 00:00:00

  • Functional Characterization of a Novel Marine Microbial Esterase and its Utilization in the Enantioselective Preparation of (R)-Methyl 2-Chloropropionate.

    abstract::Chiral 2-chloropropanoic acids and their ester derivatives are crucial intermediates in the synthesis of many chemicals, especially herbicides. The enzymatic synthesis of chiral 2-chloropropanoic acids and their ester derivatives by esterases was not easily achieved, because the structural difference between the two e...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Cao Y,Deng D,Sun A,Zhang Y,Hu Y

    更新日期:2016-09-01 00:00:00

  • An Assessment of HbA1c in Diabetes Mellitus and Pre-diabetes Diagnosis: a Multi-centered Data Mining Study.

    abstract::HbA1c test has been widely used to evaluate glycemic control in patients with diabetes. However, there are controversial results regarding the value of HbA1c in the diagnosis of diabetes mellitus (DM). The present study investigates the diagnostic effectiveness of HbA1c in a large patient group. The oral glucose toler...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,多中心研究


    authors: Serdar MA,Serteser M,Ucal Y,Karpuzoglu HF,Aksungar FB,Coskun A,Kilercik M,Ünsal İ,Özpınar A

    更新日期:2020-01-01 00:00:00

  • Comparison of the coupling recoveries of immobilized aspartate aminotransferase. Specific activity, enzyme-bound coenzyme, and transaminationable active centers.

    abstract::Aspartate aminotransferase (AspAT, EC was bound on CNBr-activated Sepharose and the effects of immobilization on the maximum velocity, biologically active pyridoxal-5'-phosphate (PLP), and transaminationable active centers were studied. By comparing these parameters of soluble and immobilized enzyme the facto...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Kurkijärvi K,Korpela T

    更新日期:1983-04-01 00:00:00

  • Diffusion and transfer of antibody proteins from a sugar-based hydrogel.

    abstract::Diffusion of antibody protein from hydrogel films and hydrogel encapsulated in a microcapillary was studied. Thin hydrogel films were formed by crosslinking 6-acryloyl-B-O-methylgalactoside with N,N'-methylene-bis-acrylamide and the diffusive transport of monoclonal antimouse IgG-FITC into and out of the hydrate films...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Markowitz MA,Turner DC,Martin BD,Gaber BP

    更新日期:1997-10-01 00:00:00

  • Natural catalytic immunity is not restricted to autoantigenic substrates: identification of a human immunodeficiency virus gp 120-cleaving antibody light chain.

    abstract::The autoimmune repertoire is well known from previous studies to be capable of producing catalytic antibodies directed to self-antigens. In the present study, we explored the ability of 26 monoclonal light chains (L chains) from multiple myeloma patients to cleave radiolabeled gp120, a foreign protein. One L chain wit...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Paul S,Kalaga RS,Gololobov G,Brenneman D

    更新日期:2000-01-01 00:00:00

  • Effect of VIB Gene on Cellulase Production of Trichoderma orientalis EU7-22.

    abstract::The cellulolytic enzymes from filamentous fungi are widely used for production of biofuels. Molecular biological engineering of fungal strains has been applied to improve the cellulase production in the recent years. The VIB gene affects production of cellulase and known as a new transcription factor. Based on a fast-...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Han J,Xue Y,Li M,Li Y,Liu J,Gan L,Long M

    更新日期:2020-08-01 00:00:00

  • Hydrogen and methane production, energy recovery, and organic matter removal from effluents in a two-stage fermentative process.

    abstract::This study evaluates the potential for using different effluents for simultaneous H(2) and CH(4) production in a two-stage batch fermentation process with mixed microflora. An appreciable amount of H(2) was produced from parboiled rice wastewater (23.9 mL g(-1) chemical oxygen demand [COD]) and vinasse (20.8 mL g(-1) ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Peixoto G,Pantoja-Filho JL,Agnelli JA,Barboza M,Zaiat M

    更新日期:2012-10-01 00:00:00

  • Magnetic microcapsules for targeted delivery of anticancer drugs.

    abstract::To achieve targeted distribution of anticancer drugs with sustained activity, ferromagnetic ethylcellulose microcapsules containing an anticancer drug, mitomycin C (FM-MMC-mc), were prepared by a method based on phase separation principles. Two prototypes of FM-MMC-mc were made: one with the drug as the core and zinc ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Kato T,Nemoto R,Mori H,Abe R,Unno K,Goto A,Murota H,Harada M,Homma M

    更新日期:1984-01-01 00:00:00

  • Lignin Sulfonation and SO2 Addition Enhance the Hydrolyzability of Deacetylated and Then Steam-Pretreated Poplar with Reduced Inhibitor Formation.

    abstract::The merit of deacetylation of corn stover prior to pretreatment is decreasing the formation of inhibitors and improving enzyme hydrolysis, proved in dilute acid pretreatment. However, few studies are done on how deacetylation would affect bioconversion process containing steam explosion. In this study, the effect of d...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Tang Y,Dou X,Hu J,Jiang J,Saddler JN

    更新日期:2018-01-01 00:00:00

  • Evaluation of Lighting Systems, Carbon Sources, and Bacteria Cultures on Photofermentative Hydrogen Production.

    abstract::Fluorescent and incandescent lighting systems were applied for batch photofermentative hydrogen production by four purple non-sulfur photosynthetic bacteria (PNSB). The hydrogen production efficiency of Rhodopseudomonas palustris, Rhodobacter sphaeroides, Rhodobacter capsulatus, and Rhodospirillum rubrum was evaluated...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Hu C,Choy SY,Giannis A

    更新日期:2018-05-01 00:00:00

  • Efficient Production of γ-GABA Using Recombinant E. coli Expressing Glutamate Decarboxylase (GAD) Derived from Eukaryote Saccharomyces cerevisiae.

    abstract::γ-Aminobutyric acid (γ-GABA) is a non-proteinogenic amino acid, which acts as a major regulator in the central nervous system. Glutamate decarboxylase (namely GAD, EC is known to be an ideal enzyme for γ-GABA production using L-glutamic acid as substrate. In this study, we cloned and expressed GAD gene from ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Xiong Q,Xu Z,Xu L,Yao Z,Li S,Xu H

    更新日期:2017-12-01 00:00:00

  • Interactions Between Autotrophic and Heterotrophic Strains Improve CO₂ Fixing Efficiency of Non-photosynthetic Microbial Communities.

    abstract::Five autotrophic strains isolated from non-photosynthetic microbial communities (NPMCs), which were screened from oceans with high CO2 fixing capability, were identified as Ochrobactrum sp. WH-2, Stenotrophomonas sp. WH-11, Ochrobactrum sp. WH-13, Castellaniella sp. WH-14, and Sinomicrobium oceani WH-15. The CO2 fixat...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Hu J,Wang L,Zhang S,Xi X,Le Y,Fu X,Tsang Y,Gao M

    更新日期:2015-07-01 00:00:00

  • Effect of surface attachment on synthesis of bacterial cellulose.

    abstract::Gluconacetobacter spp. synthesize a pure form of hydrophilic cellulose that has several industrial specialty applications. Literature reports have concentrated on intensive investigation of static and agitated culture in liquid media containing high nutrient concentrations optimized for maximal cellulose production ra...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Evans BR,O'Neill HM

    更新日期:2005-04-01 00:00:00

  • Global Transcriptome Profiling of Enterobacter Strain NRS-1 in Response to Hydrogen Peroxide Stress Treatment.

    abstract::Microbes are often subjected to oxidative stress in nature that badly affects their growth rate and viability. Although the response of microbes against oxidative stress has been characterized at the chemical, physiological, and molecular levels, the mechanism of gene-regulation network adaptations of bacteria in resp...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Fei YY,Bhat JA,Gai JY,Zhao TJ

    更新日期:2020-08-01 00:00:00