Redesign the α/β fold to enhance the stability of mannanase Man23 from Bacillus subtilis.

Abstract:

:In this work, we engineered the α/β fold of mannanase Man23 based on its molecular structure analysis to obtain more stable variants. By introducing 31 single-site mutations in the α/β fold and shuffling them, the incorporation of four mutations (K178R, K207R, N340R, and S354R) displayed a good balance between high activity and stability at higher temperature and broader pH. This quartet variant was characterized by an almost threefold increased activity and a sevenfold increased stability compared to native mannanase Man23. Our results suggest that such work is safe to increase our target protein stability with no loss of activity.

authors

Zhou HY,Pan HY,Rao LQ,Wu YY

doi

10.1007/s12010-010-9027-8

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

186-94

issue

1

eissn

0273-2289

issn

1559-0291

journal_volume

163

pub_type

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