Overproduction of glucoamylase by a deregulated mutant of a thermophilic mould Thermomucor indicae-seudaticae.

abstract：:The native amino acid ergothioneine, a thiourea derivative of histidine, inhibits mushroom tyrosinase activity in a dose-dependent manner, with an IC(50) value of 1.025 mg/ml (4.47 mM). By contrast, histidine exhibited no inhibitory effect on mushroom tyrosinase activity. We characterized ergothioneine as a noncompeti...

journal_title：Applied biochemistry and biotechnology

authors： Liao WC,Wu WH,Tsai PC,Wang HF,Liu YH,Chan CF

更新日期：2012-01-01 00:00:00

abstract：:Ethanol production was studied in simultaneous saccharification and fermentation (SSF) of steam-pretreated spruce at 42 degrees C, using a thermotolerant yeast. Three yeast strains of Kluyveromyces marxianus were compared in test fermentations. SSF experiments were performed with the best of these on 5% (w/w) of subst...

journal_title：Applied biochemistry and biotechnology

authors： Bollók M,Réczey K,Zacchi G

更新日期：2000-04-01 00:00:00

abstract：:Androstenedione (AD) is a steroid intermediate used in the pharmaceutical industry for the production of several important anabolic drugs. An important route for producing AD is by the microbial transformation of sterols. Microbes capable of efficiently transforming sterols to AD are few and newer strains need to be i...

journal_title：Applied biochemistry and biotechnology

authors： Malaviya A,Gomes J

更新日期：2009-08-01 00:00:00

abstract：:Biodiesel has become attractive due to its environmental benefits compared with conventional diesel. Although the enzymatic synthesis of biodiesel requires low thermal energy, low conversions of enzymatic transesterification with ethanol (ethanolysis) of oils to produce biodiesel are reported as a result of deactivati...

journal_title：Applied biochemistry and biotechnology

authors： Pessoa FL,Magalhães SP,Falcão PW

更新日期：2010-05-01 00:00:00

abstract：:Sweet sorghum (Sorghum sp.) has high biomass yield. Hydrolysis of lignocellulosic sweet sorghum bagasse (SSB) to fermentable sugar could be useful for manufacture of biofuel or other fermentation products. Pretreatment of lignocellulosic biomass to degrade lignin before enzymatic hydrolysis is a key step. Fungal pretr...

journal_title：Applied biochemistry and biotechnology

authors： Mishra V,Jana AK

更新日期：2017-09-01 00:00:00

abstract：:Stachybotrys microspora is a filamentous fungus characterized by the secretion of multiple hydrolytic activities (cellulolytic and non-cellulolytic enzymes). The production of these biocatalysts was studied under submerged culture using glucose, cellulose, and wheat bran as carbon sources. Endoglucanases, pectinases, ...

journal_title：Applied biochemistry and biotechnology

authors： Abdeljalil S,Ben Hmad I,Saibi W,Amouri B,Maalej W,Kaaniche M,Koubaa A,Gargouri A

更新日期：2014-02-01 00:00:00

abstract：:A study was performed regarding the effect of the relation between fill time, volume treated per cycle, and influent concentration at different applied organic loadings on the stability and efficiency of an anaerobic sequencing batch reactor containing immobilized biomass on polyurethane foam with recirculation of the...

journal_title：Applied biochemistry and biotechnology

authors： Rodrigues JA,Oliveira RP,Ratusznei SM,Zaiat M,Foresti E

更新日期：2011-01-01 00:00:00

abstract：:(R)-3, 5-Bis(trifluoromethyl)phenyl ethanol is a key chiral intermediate for the synthesis of aprepitant. Through a genome mining approach, an NADPH-dependent short-chain dehydrogenases derived from Burkholderia cenocepacia (Bc-SDR) was discovered with excellent anti-Prelog's stereoselectivity of reducing 3, 5-bis(tri...

journal_title：Applied biochemistry and biotechnology

authors： Yu S,Li H,Lu Y,Zheng G

更新日期：2018-04-01 00:00:00

abstract：:Alkaline phosphatase from hog intestine was immobilized to controlled-pore glass under various conditions. The specific activity of the enzyme was not diminished by immobilization. The influence of temperature and pH on the behavior and the stability of the immobilized enzyme preparations is discussed and compared to ...

journal_title：Applied biochemistry and biotechnology

authors： Pittner F

更新日期：1982-05-01 00:00:00

abstract：:In the present study, an intracellular melanin, named LIM205, was separated from Lachnum YM205 mycelia and was purified on a Sephadex G-15 column. The molecular weight of LIM205 was determined as 522 Da, and its molecular formula was speculated as C28H14N2O7S. The possible chemical structure of LIM205 was determined a...

journal_title：Applied biochemistry and biotechnology

authors： Zong S,Li L,Li J,Shaikh F,Yang L,Ye M

更新日期：2017-06-01 00:00:00

abstract：:Feather waste, generated in large quantities as a byproduct of commercial poultry processing, is almost pure keratin, which is not easily degradable by common proteolytic enzymes. Feather-degrading bacteria were isolated from a Brazilian poultry industrial waste. Among these isolates, a strain identified as kr2 was th...

journal_title：Applied biochemistry and biotechnology

authors： Sangali S,Brandelli A

更新日期：2000-04-01 00:00:00

abstract：:Analysis of peroxidase activity by native polyacrylamide gel electrophoresis (PAGE) from a garlic bulb (Allium sativum L) extract showed two major activities (designated POX1 and POX2). The POX2 isoenzyme was purified to homogeneity by ammonium sulfate precipitation, gel filtration, and cation-exchange chromatography....

journal_title：Applied biochemistry and biotechnology

authors： Marzouki SM,Limam F,Smaali MI,Ulber R,Marzouki MN

更新日期：2005-12-01 00:00:00

abstract：:Empty palm fruit bunch fiber (EPFBF), a readily available cellulosic biomass from palm processing facilities, is investigated as a potential carbohydrate source for cellulosic ethanol production. This feedstock was pretreated using ammonia fiber expansion (AFEX) and enzymatically hydrolyzed. The best tested AFEX condi...

journal_title：Applied biochemistry and biotechnology

authors： Lau MJ,Lau MW,Gunawan C,Dale BE

更新日期：2010-11-01 00:00:00

abstract：:Field contamination with pesticide mixtures of organophosphates (OPs) and organochlorines (OCs) is becoming global issues to be solved urgently. The strategy of utilizing engineered microorganisms that have an ability to simultaneously degrade OPs and OCs has increasingly received great interest. In this work, an OP d...

journal_title：Applied biochemistry and biotechnology

authors： Yang J,Liu R,Song W,Yang Y,Cui F,Qiao C

更新日期：2012-02-01 00:00:00

abstract：:The objective of this study was to understand the effect of pH and ionic strength of aluminum sulfate on the flocculation of microalgae. It was found that changing pH and ionic strength influenced algal flocculation by changing the zeta potential of cells, which was described by the classical theory of Derjaguin, Land...

journal_title：Applied biochemistry and biotechnology

authors： Cui Y,Yuan W,Cheng J

更新日期：2014-08-01 00:00:00

abstract：:IOGEN Corporation of Ottawa, Canada, has recently built a 40t/d biomass-to-ethanol demonstration plant adjacent to its enzyme production facility. It has partnered with the University of Toronto to test the C6/C5 cofermenta-tion performance characteristics of the National Renewable Energy Labora-tory's metabolically e...

journal_title：Applied biochemistry and biotechnology

authors： Lawford HG,Rousseau JD

更新日期：2002-04-01 00:00:00

abstract：:In this work, important results from simulations are presented, showing the potentiality of the molecular distillation process for recovering vitamin E from vegetal oils. Two types of molecular distillators are considered: falling film and centrifugal. The results emphasize the degree of recovery and factors that infl...

journal_title：Applied biochemistry and biotechnology

authors： Batistella CB,Moraes EB,Maciel Filho R,Maciel MR

更新日期：2002-04-01 00:00:00

abstract：:The ability of poly(ethylene glycol) (PEG) to protect enzymatic peroxidase activity was determined for horseradish peroxidase (HRP), versatile peroxidase (VP), commercial Coprinus peroxidase (BP), and chloroperoxidase (CPO). The operational stability measured as the total turnover number was determined for the four pe...

journal_title：Applied biochemistry and biotechnology

authors： Juarez-Moreno K,Ayala M,Vazquez-Duhalt R

更新日期：2015-11-01 00:00:00

abstract：:In the present study, effects of aqueous extracts from Crocodylus siamensis bile (AE-CB) on SMMC-7721 cell growth, cell cycle, and apoptosis were investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, inverted microscopy, fluorescence microscopy, propidium iodide (PI) single- and fluoresc...

journal_title：Applied biochemistry and biotechnology

authors： Song W,Li SS,Qiu PP,Shen DY,Tian L,Zhang QY,Liao LX,Chen QX

更新日期：2013-05-01 00:00:00

abstract：:The two six-coordinate Pt(IV) complexes, containing bidentate nitrogen donor/methyl ligands with general formula [Pt(X)2Me2((t)bu2bpy)], where (t)bu2bpy = 4,4'-ditert-butyl-2,2'-bipyridine and X = Cl (C1) or Br (C2), serving as the leaving groups were synthesized for evaluation of their anticancer activities and DNA b...

journal_title：Applied biochemistry and biotechnology

authors： Pouryasin Z,Yousefi R,Nabavizadeh SM,Rashidi M,Hamidizadeh P,Alavianmehr MM,Moosavi-Movahedi AA

更新日期：2014-03-01 00:00:00

abstract：:Extracellular polymeric substances (EPS) produced by a biofilm-producing bacterium Acinetobacter junii BB1A were characterized. Purified EPS on analysis revealed neutral sugar (73.21 %), uronic acid (10.12 %), amino sugars (0.23 %), α- amino acids (11.13 %), and aromatic amino acids (1.23 %). Infrared spectrometry rev...

journal_title：Applied biochemistry and biotechnology

authors： Yadav KK,Mandal AK,Sen IK,Chakraborti S,Islam SS,Chakraborty R

更新日期：2012-11-01 00:00:00

abstract：:The capacity for anaerobic decolorization of a sulfonated azo dye, Congo Red, by a strain of a sulfate-reducing bacterium was evaluated. After optimizing the growth rate of the bacteria on a simple carbon source and terminal electron acceptor pair, lactate and sulfate, respectively, the effect of the dye concentration...

journal_title：Applied biochemistry and biotechnology

authors： Diniz PE,Lopes AT,Lino AR,Serralheiro ML

更新日期：2002-03-01 00:00:00

abstract：:Proteases are some of the most important industrial enzymes, and one of their main applications is for the production of cheese in the dairy industry. Due to a shortage of animal rennet, microbial coagulant proteases are being sought. In this work, the production of microbial rennet from Thermomucor indicae-seudaticae...

journal_title：Applied biochemistry and biotechnology

authors： Silva BL,Geraldes FM,Murari CS,Gomes E,Da-Silva R

更新日期：2014-02-01 00:00:00

abstract：:Recombinant tissue-plasminogen activator (r-tPA), expressed in Escherichia coli cells in an aggregated form, was solubilized with a strong chaotrope in the absence of any reducing agent. The solubilized molecule was reactivated by a procedure that was developed to mimic the physiological conditions optimal for the fun...

journal_title：Applied biochemistry and biotechnology

authors： Grunfeld H,Patel A,Shatzman A,Nishikawa AH

更新日期：1992-05-01 00:00:00

abstract：:The transesterification of soybean lecithin with ethyl esters of polyunsaturated fatty acids (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) using immobilized lipase from Rhizomucor miehei was tested in the presence or absence of organic solvent (hexane) and additives (urea with Ca(2+) or Mg(2+)). The rea...

journal_title：Applied biochemistry and biotechnology

authors： Marsaoui N,Naghmouchi K,Baah J,Raies A,Laplante S

更新日期：2015-06-01 00:00:00

abstract：:The short supply of edible vegetable oils is the limiting factor in the progression of biodiesel technology; thus, in this study, we applied response surface methodology in order to optimize the reaction factors for biodiesel synthesis from inedible castor oil. Specifically, we evaluated the effects of multiple parame...

journal_title：Applied biochemistry and biotechnology

authors： Jeong GT,Park DH

更新日期：2009-05-01 00:00:00

abstract：:A DNA fragment of 2,042 bp containing a novel beta-mannanase gene, man5A, was identified from the genome of the mannan-degrading bacterium Bacillus circulans CGMCC1554. The open reading frame of man5A comprised 978 bp encoding a protein of 326 amino acids with a predicted molecular weight of 32 kDa. The amino acid seq...

journal_title：Applied biochemistry and biotechnology

authors： Yang P,Li Y,Wang Y,Meng K,Luo H,Yuan T,Bai Y,Zhan Z,Yao B

更新日期：2009-10-01 00:00:00

abstract：:A soluble glucoside 3-dehydrogenase (G3DH) was purified from a newly isolated Sphingobacterium faecium ZJF-D6 CCTCC M 2013251. The enzyme was purified to 35.71-fold with a yield of 41.91 % and was estimated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis with a molecular mass of 62 kDa. The sequences of ...

journal_title：Applied biochemistry and biotechnology

authors： Zhang JF,Yang B,Chen W,Chen J

更新日期：2014-04-01 00:00:00

abstract：:In this presented work, magnetic poly(HEMA-GMA) nanoparticles were synthesized, characterized, and used for immobilization of an anti-leukemic enzyme L-asparaginase. The average particle size of the synthesized magnetic nanoparticles was found to be as 117.5 nm. L-asparaginase was successfully immobilized onto the syn...

journal_title：Applied biochemistry and biotechnology

authors： Orhan H,Aktaş Uygun D

更新日期：2020-08-01 00:00:00

abstract：:Residue of chili plants left in the field after harvesting is a major lignocellulosic resource that is underexploited. India has over 0.6 million tons of this residue available as surplus annually which projects it as a potent feedstock for conversion to bioethanol. The cellulose, hemicellulose and lignin content of t...

journal_title：Applied biochemistry and biotechnology

authors： Preeti VE,Sandhya SV,Kuttiraja M,Sindhu R,Vani S,Kumar SR,Pandey A,Binod P

更新日期：2012-07-01 00:00:00