Effector-assisted refolding of recombinant tissue-plasminogen activator produced in Escherichia coli.

abstract：:The host-pathogen interaction and involvement of calcium (Ca2+) signaling in tuberculosis infection is crucial and plays a significant role in pathogenesis. Ca2+ is known as a ubiquitous second messenger that could control multiple processes and is included in cellular activities like division, motility, stress respon...

journal_title：Applied biochemistry and biotechnology

authors： Sharma S,Meena LS

更新日期：2017-02-01 00:00:00

abstract：:A Bacillus subtilis strain isolated from contaminated soil from a refinery has been screened for biosurfactant production in crystal sugar (sucrose) with different nitrogen sources (NaNO(3), (NH(4))(2)SO(4), urea, and residual brewery yeast). The highest reduction in surface tension was achieved with a 48-h fermentati...

journal_title：Applied biochemistry and biotechnology

authors： Fonseca RR,Silva AJ,De França FP,Cardoso VL,Sérvulo EF

更新日期：2007-04-01 00:00:00

abstract：:Lignocellulosic biomass has great potential as a cheap feedstock in biological processes to produce biofuels or chemicals; however, dilute acid pretreatment at high temperatures produces undesirable compounds. Toxicity tests were done with inhibitors in standard media, to predict the growth-limiting effects on thermop...

journal_title：Applied biochemistry and biotechnology

authors： Thomasser C,Danner H,Neureiter M,Saidi B,Braun R

更新日期：2002-04-01 00:00:00

abstract：:This study investigated the effects of flask-to-liquid volume ratio on the growth of Panax ginseng hairy root, transformed by Agrobacterium rhizogenes, in flask cultures and compared the characteristics of various bioreactors for scale-up. The flask-to-liquid volume ratio was optimum at 1.5 mL of air/mL of medium in f...

journal_title：Applied biochemistry and biotechnology

authors： Jeong GT,Park DH,Hwang B,Woo JC

更新日期：2003-04-01 00:00:00

abstract：:The presence of high strength fats and oils in dairy industry wastewaters poses serious challenges for biological treatment systems, and, therefore, its pretreatment is necessary in order to remove them. In the present study, synthetic dairy wastewater prepared in the laboratory was pretreated using the sophorolipid-p...

journal_title：Applied biochemistry and biotechnology

authors： Daverey A,Pakshirajan K

更新日期：2011-03-01 00:00:00

abstract：:A high cellobiohydrolase (CBH)-producing strain was isolated and identified as Penicillium purpurogenum KJS506 according to the morphology and comparison of internal transcribed spacer rDNA gene sequence. When rice straw and corn steep powder were used as carbon and nitrogen sources, respectively, a maximum CBH activi...

journal_title：Applied biochemistry and biotechnology

authors： Lee KM,Joo AR,Jeya M,Lee KM,Moon HJ,Lee JK

更新日期：2011-01-01 00:00:00

abstract：:We have previously demonstrated that pretreatment of corn stover with dilute sulfuric acid can achieve high digestibility and efficient recovery of hemicellulose sugars with high yield and concentration. Further improvement of this process was sought in this work. A modification was made in the operation of the percol...

journal_title：Applied biochemistry and biotechnology

authors： Zhu Y,Lee YY,Elander RT

更新日期：2005-04-01 00:00:00

abstract：:The epithelial-mesenchymal transition (EMT) is a process in which epithelial cells lose their cell polarity and cell-cell adhesion, and gain migratory and invasive properties. It is believed that EMT is associated with initiation and completion of the invasion-metastasis cascade. In this study, an economic approach wa...

journal_title：Applied biochemistry and biotechnology

authors： Li Y,Gao A,Yu L

更新日期：2016-01-01 00:00:00

abstract：:An anaerobic sequencing batch reactor containing immobilized biomass (AnSBBR) was used to produce biomethane by treating the effluent from another AnSBBR used to produce biohydrogen from glucose- (AR-EPHG) and sucrose-based (AR-EPHS) wastewater. In addition, biomethane was also produced from sucrose-based synthetic wa...

journal_title：Applied biochemistry and biotechnology

authors： Lullio TG,Souza LP,Ratusznei SM,Rodrigues JA,Zaiat M

更新日期：2014-11-01 00:00:00

abstract：:Four endoglucanase temperature isoforms (T (30), T (50), T (70), and T (90)) were identified and purified from the cladodes of the xerophytic plant Opuntia vulgaris. These isoforms exhibited optimum catalytic activity at 30 °C, 50 °C, 70 °C, and 90 °C and yielded an apparent molecular mass of 150, 20, 74, and 45 kDa, ...

journal_title：Applied biochemistry and biotechnology

authors： Shyamala S,Ravikumar S,Vikramathithan J,Srikumar K

更新日期：2011-12-01 00:00:00

abstract：:The present study deals with the characterization of halotolerant protease produced by Bacillus aquimaris VITP4 strain isolated from Kumta coast, Karnataka, India. The studies were performed at 40 °C and pH 8 in Tris buffer. Metal ions such as Mn(2+) and Ca(2+) increased the proteolytic activity of the enzyme by 34 an...

journal_title：Applied biochemistry and biotechnology

authors： Thaz CJ,Jayaraman G

更新日期：2014-01-01 00:00:00

abstract：:Field contamination with pesticide mixtures of organophosphates (OPs) and organochlorines (OCs) is becoming global issues to be solved urgently. The strategy of utilizing engineered microorganisms that have an ability to simultaneously degrade OPs and OCs has increasingly received great interest. In this work, an OP d...

journal_title：Applied biochemistry and biotechnology

authors： Yang J,Liu R,Song W,Yang Y,Cui F,Qiao C

更新日期：2012-02-01 00:00:00

abstract：:In plants, the final step of cysteine formation is catalyzed by O-acetylserine (thiol) lyase (OAS-TL). The purpose of this study was to isolate and characterize an OAS-TL from the tree legume Leucaena leucocephala (leucaena). Leucaena contains a toxic, nonprotein amino acid, mimosine, which is also formed by an OAS-TL...

journal_title：Applied biochemistry and biotechnology

authors： Yafuso JT,Negi VS,Bingham JP,Borthakur D

更新日期：2014-07-01 00:00:00

abstract：:Proteases constitute one of the most important groups of industrial enzymes, accounting for at least 25% of the total enzyme sales, with two-thirds of the proteases produced commercially being of microbial origin. Immobilized enzymes are currently the subject of considerable interest because of their advantages over s...

journal_title：Applied biochemistry and biotechnology

authors： Kirkkopru I,Alpaslan C,Omay D,Güvenilir Y

更新日期：2006-04-01 00:00:00

abstract：:During glycerol fed-batch fermentation, the process could be divided into multiple equal subintervals, and the feed operation was performed in pulse form at the start of each subinterval. Based on the macrokinetic models, the multipulse feed strategy for both glucose and corn steep slurry was determined by a general n...

journal_title：Applied biochemistry and biotechnology

authors： Xie D,Liu D,Zhu H,Liu T

更新日期：2001-08-01 00:00:00

abstract：:Lysozyme was immobilized by two different methods in two different ways in order to obtain a preparation with an activity as high as possible toward a macromolecular substrate. The enzyme was bound as a Schiff base to a silicate carrier by using oxidized dextrans of different lengths as spacer and also was bound to co...

journal_title：Applied biochemistry and biotechnology

authors： Moser I,Dworsky P,Pittner F

更新日期：1988-12-01 00:00:00

abstract：:Fatty acid methyl esters (FAMEs) show large potential applications as diesel substitutes, also known as biodiesel fuel. Biodiesel fuel as renewable energy is an alternative that can reduce energy dependence on petroleum as well as air pollution. Several processes for the production of biodiesel fuel have been develope...

journal_title：Applied biochemistry and biotechnology

authors： Jeong GT,Park DH,Kang CH,Lee WT,Sunwoo CS,Yoon CH,Choi BC,Kim HS,Kim SW,Lee UT

更新日期：2004-04-01 00:00:00

abstract：:Entrapped cells of P. diminuta were used for the production of 7-aminocephalosporanic acid (7-ACA), a key intermediate required for the production of most of the clinically used cephalosporin derivatives, i.e., semisynthetic cephalosporins. The repeated batch production of 7-ACA with entrapped cells of P. diminuta in ...

journal_title：Applied biochemistry and biotechnology

authors： Nigam VK,Kundu S,Ghosh P

更新日期：2007-04-01 00:00:00

abstract：:Vanillin is one of the most widely used flavoring agents in the world. As the annual world market demand of vanillin could not be met by natural extraction, chemical synthesis, or tissue culture technology, thus biotechnological approaches may be replacement routes to make production of bio-vanillin economically viabl...

journal_title：Applied biochemistry and biotechnology

authors： Kaur B,Chakraborty D

更新日期：2013-02-01 00:00:00

abstract：:Tradescantia pallida (Wandering jew)-a succulent perennial herb-was screened to be a potent chromium (Cr) accumulator. Its ability to grow under Cr stress was examined by studying biochemical changes and physiological response of the plant in presence of 5-20 mg L(-1) Cr(VI) concentration in hydroponic environment for...

journal_title：Applied biochemistry and biotechnology

authors： Sinha V,Pakshirajan K,Chaturvedi R

更新日期：2014-08-01 00:00:00

abstract：:This research aimed at developing and designing a slow and targeted delivery of Amphotericin B (AmB) antibiotic by placing three types of shells containing different ratios of biodegradable and biocompatible polymers poly (D, L-lactide)-co-(glycolide) (PLGA), polyethylene glycol (PEG), and polyvinyl pyrrolidone (PVP) ...

journal_title：Applied biochemistry and biotechnology

authors： Sadat Akhavi S,Moradi Dehaghi S

更新日期：2020-06-01 00:00:00

abstract：:Polyhydroxyalkanoates, biodegradable plastics with the desired physical and chemical properties of conventional synthetic plastics, are extensively investigated. In this study, specific bacterial strains produced specific copolymers from food waste. Copolymers of HB and HV (poly[3-hydroxybutyrate-co-3-hydroxyvalerate]...

journal_title：Applied biochemistry and biotechnology

authors： Wong PA,Chua H,Lo W,Lawford HG,Yu PH

更新日期：2002-04-01 00:00:00

abstract：:Solid-state fermentation, featured by water-saving, eco-friendly and high concentration product, is a promising technology in lignocellulosic ethanol industry. However, in solid-state fermentation system, large gas content inside the substrate directly leads to high oxygen partial pressure and inhibits ethanol ferment...

journal_title：Applied biochemistry and biotechnology

authors： Sun L,Wang L,Chen H

更新日期：2020-10-01 00:00:00

abstract：:The rheological behavior of a sulfated galactan extracted from Halymenia durvillei, a red seaweed collected in the coastal waters of a small island of Madagascar (Nosy-be in Indian Ocean), was investigated in dilute and semi-dilute solutions. In dilute solution with NaCl at 0.3 M, the polysaccharide adopted a coil con...

journal_title：Applied biochemistry and biotechnology

authors： Fenoradosoa TA,Laroche C,Delattre C,Dulong V,Le Cerf D,Picton L,Michaud P

更新日期：2012-07-01 00:00:00

abstract：:Apoptotic cell death is a fundamental process in the development and physiological homeostasis of multicellular organisms. It is associated with control of cell numbers in tissues and organs during development, with cell turnover, and with response to infection. Molecules that trigger this process in continuously prol...

journal_title：Applied biochemistry and biotechnology

authors： Das MK,Sharma RS,Mishra V

更新日期：2012-03-01 00:00:00

abstract：:The vegetative insecticidal proteins (VIPs) of Bacillus thuringiensis (Bt) have a broad-spectrum insecticidal activity against Lepidopteran pests and no cross-resistance with the insecticidal crystal protein Cry protein. So there are great potentials for the control of agricultural pests and the resolution of resistan...

journal_title：Applied biochemistry and biotechnology

authors： Chi B,Li H,Zhang J,Wei P,Gao J,Liu R

更新日期：2019-04-01 00:00:00

abstract：:More than two decades ago, a group of research engineers and applied scientists with interest in energy applications of bioprocessing initiated an annual symposium series ultimately entitled "Biotechnology for Fuels and Chemicals." The Department of Energy, several of the national laboratories, and various industrial ...

journal_title：Applied biochemistry and biotechnology

authors： Scott CD

更新日期：2004-04-01 00:00:00

abstract：:Lignocellulose biomass contain large macromolecules especially cellulose and hemicelluloses that can be converted to fuel and chemicals using microbial biocatalysts. This study presents comprehensive optimization of production of biomass-hydrolyzing enzymes (BHE) by a high β-glucosidase-producing Trichoderma SG2 for b...

journal_title：Applied biochemistry and biotechnology

authors： Nanjundaswamy A,Okeke BC

更新日期：2020-05-01 00:00:00

abstract：:An extracellular low molecular weight xylanase (Xyn628) from Streptomyces sp. CS628 was isolated from Korean soil sample, produced in wheat bran medium, purified, and biochemically characterized. Xyn628 was purified 4.8-fold with a 33.78 % yield using Sepharose CL-6B column chromatography. The purified xylanase was ~1...

journal_title：Applied biochemistry and biotechnology

authors： Rahman MA,Choi YH,Pradeep GC,Choi YS,Choi EJ,Cho SS,Yoo JC

更新日期：2014-07-01 00:00:00

abstract：:We explored the influence of dilution rate and pH in continuous cultures of Clostridium acetobutylicum. A 200-mL fibrous bed bioreactor was used to produce high cell density and butyrate concentrations at pH 5.4 and 35degreesC. By feeding glucose and butyrate as a cosubstrate, the fermentation was maintained in the so...

journal_title：Applied biochemistry and biotechnology

authors： Huang WC,Ramey DE,Yang ST

更新日期：2004-04-01 00:00:00