Isolation, purification, and characterization of two thermostable endo-1,4-β-D-glucanase forms from Opuntia vulgaris.

Abstract:

:Four endoglucanase temperature isoforms (T (30), T (50), T (70), and T (90)) were identified and purified from the cladodes of the xerophytic plant Opuntia vulgaris. These isoforms exhibited optimum catalytic activity at 30 °C, 50 °C, 70 °C, and 90 °C and yielded an apparent molecular mass of 150, 20, 74, and 45 kDa, respectively, on gel filtration chromatography. These isoforms were purified 24-, 25-, 29-, and 27-fold with a yield of 15%, 12%, 17%, and 19% and having a specific activity of 120, 125, 144, and 136 U/mg, respectively. The thermostable T (70) and T (90) isoforms exhibited optimum activity at pH 4.5 and 7 and also yielded a molecular weight of 66 and 36 kDa, respectively, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The T (70) had a K (m) of 43 mM and a V (max) of 12.5 μmol min(-1) μg(-1) of protein, and the T (90) isoform had a K (m) of 40 mM, with an apparent V (max) of 10 μmol min(-1) μg(-1) of protein. Western blot, immunodiffusion, and in vitro inhibition assays established the reactivity of the T (90) isoform with polyclonal anti-T (90) antibody raised in rabbit. Cross-reactivity of this antibody with the T (70) endoglucanase isoform was also noted.

authors

Shyamala S,Ravikumar S,Vikramathithan J,Srikumar K

doi

10.1007/s12010-011-9380-2

subject

Has Abstract

pub_date

2011-12-01 00:00:00

pages

1597-610

issue

7-8

eissn

0273-2289

issn

1559-0291

journal_volume

165

pub_type

杂志文章
  • Optimization of fermentation conditions for production of glycopeptide antibiotic vancomycin by Amycolatopsis orientalis.

    abstract::Glycopeptides produced by Streptomyces species are the drugs used against beta-lactam drug-resistant staphylococcal infections, and vancomycin is important among them. Increased prevalence of resistant strains increased the usage of vancomycin worldwide and also promoted attempts for indigenous production. The optimum...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:102-103:1-6:395

    authors: Padma PN,Rao AB,Yadav JS,Reddy G

    更新日期:2002-07-01 00:00:00

  • Use of Swine Wastewater as Alternative Substrate for Mycelial Bioconversion of White Rot Fungi.

    abstract::Seven white rot fungal species were tested for growth as mycelia using swine wastewater (SW), an agro-waste with tremendous environmental footprint, as the sole nutrient source. The SW contained high concentrations of carbon and nitrogen components, which could support nutritional requirements for mycelial growth. Out...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-016-2253-y

    authors: Lee J,Shin SG,Ahn J,Han G,Hwang K,Kim W,Hwang S

    更新日期:2017-02-01 00:00:00

  • A unique feature of hydrogen recovery in endogenous starch-to-alcohol fermentation of the marine microalga, Chlamydomonas perigranulata.

    abstract::A unicellular marine green alga, Chlamydomonas perigranulata, was demonstrated to synthesize starch through photosynthesis, store it in a cell, and ferment it under anaerobic conditions in the dark to produce ethanol, 2,3-butanediol (butanediol), acetic acid, and carbon dioxide (CO2). Previous fermentation data of an ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:

    authors: Hon-Nami K

    更新日期:2006-04-01 00:00:00

  • Bioconversion of sodium dodecyl sulphate to rhamnolipid by Pseudomonas aeruginosa: a novel and cost-effective production strategy.

    abstract::The utility of rhamnolipids in industry is currently limited due to the high constraints in its economic production. In this scenario, the novel utility of sodium dodecyl sulphate (SDS) as carbon source could serve as promising cost-effective strategy. Screening of effective SDS biodegraders led to the isolation of Ps...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-9988-x

    authors: Rebello S,Asok AK,Joseph SV,Joseph BV,Jose L,Mundayoor S,M S J

    更新日期:2013-01-01 00:00:00

  • A simple substrate feeding strategy using a pH control trigger in fed-batch fermentation.

    abstract::A simple automated glucose feeding strategy based on pH control was developed to produce high-cell-density fed-batch fermentation. In this strategy, the pH control scheme utilized an acidified concentrated glucose solution to lower the pH. The frequency of glucose addition to the fermentor is determined by the culture...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-007-8089-8

    authors: Ting TE,Thoma GJ,Beitle RR Jr,Davis RK,Perkins R,Karim K,Liu HM

    更新日期:2008-04-01 00:00:00

  • Expression and characterization of recombinant GH11 xylanase from thermotolerant Streptomyces sp. SWU10.

    abstract::Xylans are major hemicellulose components of plant cell wall which can be hydrolyzed by xylanolytic enzymes. Three forms of endo-β-1,4-xylanases (XynSW1, XynSW2A, and XynSW2B) produced by thermotolerant Streptomyces sp. SWU10 have been reported. In the present study, we described the expression and characterization of...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0508-4

    authors: Sukhumsirichart W,Deesukon W,Kawakami T,Matsumoto S,Seesom W,Sakamoto T

    更新日期:2014-01-01 00:00:00

  • Properties of a thermostable nonspecific fructofuranosidase produced by Cladosporium cladosporioides cells for hydrolysis of Jerusalem artichoke extract.

    abstract::Thermostable invertase (E.C. 3.2.1.26) and inulinase 2,1-beta-D-fructan fructanohydrolase (E.C. 3.2.1.7) activities were produced by Cladosporium cladosporioides grown on sucrose, inulin, yam extract, or Jerusalem artichoke. The ratio I (inulinase)/S(invertase) activity was between 0.31 and 0.36. Both activities had h...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02922120

    authors: Ferreira MS,De Andrade AV,Kennedy JF

    更新日期:1991-10-01 00:00:00

  • Production of herbicide-resistant medicinal plant Salvia miltiorrhiza transformed with the bar gene.

    abstract::In this study, we successfully performed Agrobacterium-mediated genetic transformation of Salvia miltiorrhiza and produced herbicide-resistant transformants. Leaf discs of S. miltiorrhiza were infected with Agrobacterium tumefaciens EHA105 harboring pCAMBIA 3301. The pCAMBIA 3301 includes an intron-containing gus repo...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-015-1826-5

    authors: Liu Y,Yang SX,Cheng Y,Liu DQ,Zhang Y,Deng KJ,Zheng XL

    更新日期:2015-12-01 00:00:00

  • Lignin Sulfonation and SO2 Addition Enhance the Hydrolyzability of Deacetylated and Then Steam-Pretreated Poplar with Reduced Inhibitor Formation.

    abstract::The merit of deacetylation of corn stover prior to pretreatment is decreasing the formation of inhibitors and improving enzyme hydrolysis, proved in dilute acid pretreatment. However, few studies are done on how deacetylation would affect bioconversion process containing steam explosion. In this study, the effect of d...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-017-2545-x

    authors: Tang Y,Dou X,Hu J,Jiang J,Saddler JN

    更新日期:2018-01-01 00:00:00

  • Effects of pulsed electric field on secondary metabolism of Vitis vinifera L. cv. Gamay Fréaux suspension culture and exudates.

    abstract::Plant cell cultures provide a large potential for the production of secondary metabolites. Through the application of different physical and chemical cell stress factors, we investigated the production of the secondary metabolites in plant cell cultures. The effects of pulsed electric field (PEF) and ethephon on growt...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-010-9146-2

    authors: Cai Z,Riedel H,Thaw Saw NM,Kütük O,Mewis I,Jäger H,Knorr D,Smetanska I

    更新日期:2011-06-01 00:00:00

  • On-line characterization of physiological state in poly(beta-hydroxybutyrate) production by Wautersia eutropha.

    abstract::Culture fluorescence measurement technique has the potential for on-line characterization of metabolic status of fermentation processes. Many fluorophores present inside the living cells such as NADH + H+, tryptophan, pyridoxine, and riboflavin fluoresce at specific excitation and emission wavelength combinations. Sin...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-008-8395-9

    authors: Khanna S,Srivastava AK

    更新日期:2009-05-01 00:00:00

  • Effective purification of Cerrena unicolor laccase using microfiltration, ultrafiltration and acetone precipitation.

    abstract::Microfiltration followed by concentration and diafiltration on ultrafiltration membranes (Biomax-100, Biomax-10 and Ultracel-10) was used to recover extracellular laccase (EC 1.10.3.2) from culture broth of wood-rotting fungus Cerrena unicolor. Feed, permeate, retentate and membrane wash-out solutions were analysed fo...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8791-9

    authors: Bryjak J,Rekuć A

    更新日期:2010-04-01 00:00:00

  • Biotechnological storage and utilization of entrapped solar energy.

    abstract::Our laboratory has recently developed a device employing immobilized F0F1 adenosine triphosphatase (ATPase) that allows synthesis of adenosine triphosphate (ATP) from adenosine 5'-diphosphate and inorganic phosphate using solar energy. We present estimates of total solar energy received by Earth's land area and demons...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:120:3:159

    authors: Bhattacharya S,Schiavone M,Nayak A,Bhattacharya SK

    更新日期:2005-03-01 00:00:00

  • Scale up and application of biosurfactant from Bacillus subtilis in Enhanced Oil recovery.

    abstract::There is a lack of fundamental knowledge about the scale up of biosurfactant production. In order to develop suitable technology of commercialization, carrying out tests in shake flasks and bioreactors was essential. A reactor with integrated foam collector was designed for biosurfactant production using Bacillus subt...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8889-0

    authors: Amani H,Mehrnia MR,Sarrafzadeh MH,Haghighi M,Soudi MR

    更新日期:2010-09-01 00:00:00

  • Mobilization and biodegradation of 2-methylnaphthalene by amphiphilic polyurethane nano-particle.

    abstract::Amphiphilic polyurethane (APU) nano-particle enhanced the mobilization of 2-methylnaphthalene (2-MNPT) in soil. Significant increase in the solubility of 2-MNPT was achieved. The molar solubilization ratio was 0.4 (mole 2-MNPT/mole APU). Simple precipitation of APU particle by 2 N CaCl(2) recovered 95% of APU particle...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8550-y

    authors: Kim YB,Kim JY,Kim EK

    更新日期:2009-10-01 00:00:00

  • The effect of hydrogen ion on the steady-state multiplicity of substrate-inhibited enzymatic reactions. II. Transient behavior.

    abstract::In this paper we concentrate our attention on the stability and transient behavior of the isothermal system (CSTR) with a substrate-inhibited enzyme reaction producing hydrogen ions. Our investigation covers the region of multiple steady states uncovered previously (1) (ordinary hysteresis and isola). We investigate t...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02780380

    authors: Elnashaie SS,Elrifaie MA,Ibrahim G,Badra G

    更新日期:1983-12-01 00:00:00

  • Sorbitol can be produced not only chemically but also biotechnologically.

    abstract::Sorbitol, a polyol found in many fruits, is attracting increasing industrial interest as a sweetener, humectant, texturizer, and softener. It is principally produced by chemical means. The bacterium Zymomonas mobilis is able to produce sorbitol together with gluconic acid from fructose and glucose, respectively. This ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1385/abab:118:1-3:321

    authors: Jonas R,Silveira MM

    更新日期:2004-07-01 00:00:00

  • Development and application of a system for analysis of mixed cultures of microorganisms.

    abstract::Development and application of a system for real-time quantitative assessment of individual cell activities in a mixed culture system was investigated. This was based on a concept that the activities of individual cells in a mixed culture can be assessed if the cells are physically separated (in separate compartments)...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:80:1:51

    authors: Tanaka H,Ebata T,Kuwahara I,Matsuo M,Ogbonna JC

    更新日期:1999-04-01 00:00:00

  • Ultrastructural and intracellular chemical changes of a novel halophilic strain V430 of Staphylococcus saprophyticus under CaCl2 stress.

    abstract::Observation of the ultrastructural and intracellular chemical changes of CaCl2-tolerant strains is important both for understanding their adaptation mechanism under high salt stress and for providing theoretical basis of their application in treating high-CaCl2 wastewaters. A novel strain V 430 isolated by us has been...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-007-0038-z

    authors: Xin X,Wang Y

    更新日期:2007-09-01 00:00:00

  • Modeling and computational simulation of dilution and biochemical materials balance equations for partially emptied batch reactors.

    abstract::Sequencing batch reactors (SBRs) including aerobic SBRs and anaerobic SBRs (ASBRs) are partially emptied batch reactors that are widely used as bioprocesses in pollution control. We present dilution and biochemical materials balance modeling equations and simulation results for the partially emptied batch reactors, es...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02685941

    authors: Ndon UJ,Zhao JC,Siguan ML

    更新日期:2007-01-01 00:00:00

  • Optimization of L-lactic acid production from glucose by Rhizopus oryzae ATCC 52311.

    abstract::The effect of nutrients on L(+)-lactic acid production from glucose was investigated using Rhizopus oryzae ATCC 52311. From the shake-flask experiments, the optimal medium composition was defined for improved lactic-acid production. In order to enhance lactic-acid production rate and product yield, controlled aeration...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:78:1-3:401

    authors: Zhou Y,Domínguez JM,Cao N,Du J,Tsao GT

    更新日期:1999-04-01 00:00:00

  • Determining yields in high solids enzymatic hydrolysis of biomass.

    abstract::As technologies for utilizing biomass for fuel and chemical production continue to improve, enzymatic hydrolysis can be run at still higher solids concentrations. For hydrolyses that initially contain little or no free water (10-40% total solids, w/w), the saccharification of insoluble polymers into soluble sugars inv...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-008-8375-0

    authors: Kristensen JB,Felby C,Jørgensen H

    更新日期:2009-05-01 00:00:00

  • Efficient Antibody Assembly in E. coli Periplasm by Disulfide Bond Folding Factor Co-expression and Culture Optimization.

    abstract::Molecular chaperones and protein folding factors of bacterial periplasmic space play important roles in assisting disulfide bond formation and proper protein folding. In this study, effects of disulfide bond protein (Dsb) families were investigated on assembly of 3F3 Fab, an antibody inhibitor targeting matrix metallo...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-017-2502-8

    authors: Rodriguez C,Nam DH,Kruchowy E,Ge X

    更新日期:2017-10-01 00:00:00

  • Effects of organic loading, influent concentration, and feed time on biohydrogen production in a mechanically stirred AnSBBR treating sucrose-based wastewater.

    abstract::An anaerobic sequencing batch biofilm reactor (AnSBBR-total volume 7.5 L; liquid volume 3.6 L; treated volume per cycle 1.5 L) treated sucrose-based wastewater to produce biohydrogen (at 30 °C). Different applied volumetric organic loads (AVOL of 9.0, 12.0, 13.5, 18.0, and 27.0 kg COD m(-3) day(-1)), which were varied...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0457-y

    authors: Manssouri M,Rodrigues JA,Ratusznei SM,Zaiat M

    更新日期:2013-12-01 00:00:00

  • Expression of codon-optmized phosphoenolpyruvate carboxylase gene from Glaciecola sp. HTCC2999 in Escherichia coli and its application for C4 chemical production.

    abstract::We examined the expression of the phosphoenolpyruvate carboxylase (PEPC) gene from marine bacteria in Escherichia coli using codon optimization. The codon-optimized PEPC gene was expressed in the E. coli K-12 strain W3110. SDS-PAGE analysis revealed that the codon-optimized PEPC gene was only expressed in E. coli, and...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-9730-8

    authors: Park S,Pack SP,Lee J

    更新日期:2012-08-01 00:00:00

  • Functional Characterization of a Robust Marine Microbial Esterase and Its Utilization in the Stereo-Selective Preparation of Ethyl (S)-3-Hydroxybutyrate.

    abstract::One novel microbial esterase PHE21 was cloned from the genome of Pseudomonas oryzihabitans HUP022 identified from the deep sea of the Western Pacific. PHE21 was heterologously expressed and functionally characterized to be a robust esterase which behaved high resistance to various metal ions, organic solvents, surfact...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-016-2161-1

    authors: Wang Y,Zhang Y,Hu Y

    更新日期:2016-11-01 00:00:00

  • Prediction of folding stability and degradability of the de novo designed protein MB-1 in cow rumen.

    abstract::The authors have recently reported on the design of a protein (MB-1) enriched in methionine, threonine, lysine, and leucine. The protein is intended to be produced by rumen bacteria, in a way that would provide high producing lactating cows with limiting amino acids. In this report, MB-1 stability in the rumen is asse...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02788809

    authors: MacCallum JD,Hefford MA,Omar S,Beauregard M

    更新日期:1997-04-01 00:00:00

  • Study on the production of a xylanolytic complex from Penicillium canescens 10-10c.

    abstract::We screened about a hundred microorganisms (including unidentified yeasts, fungi, and bacteria) for their ability to produce xylanolytic enzymes. About 40 of them were hemicellulolytic; among these, we selected Penicillium canescens 10-10c for detailed study because of its ability to produce an interesting enzymatic c...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02787840

    authors: Gaspar A,Cosson T,Roques C,Thonart P

    更新日期:1997-07-01 00:00:00

  • Biological N removal from wastes generated from amine-based CO2 capture: case monoethanolamine.

    abstract::Large-scale amine-based CO(2) capture will generate waste containing large amounts of ammonia, in addition to contaminants such as the actual amine as well as degradation products thereof. Monoethanolamine (MEA) has been a dominant amine applied so far in this context. This study reveals how biological N removal can b...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-0075-0

    authors: Hauser I,Colaço AB,Skjæran JA,Einbu A,Ostgaard K,Svendsen HF,Cervantes FJ

    更新日期:2013-02-01 00:00:00

  • Rhizopus oryzae lipase immobilized on hierarchical mesoporous silica supports for transesterification of rice bran oil.

    abstract::The tunable textural properties of self-oriented mesoporous silica were investigated for their suitability as enzyme immobilization matrices to support transesterification of rice bran oil. Different morphologies of mesoporous silica (rod-like, rice-like, and spherical) were synthesized and characterized by scanning e...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-1432-y

    authors: Ramachandran P,Narayanan GK,Gandhi S,Sethuraman S,Krishnan UM

    更新日期:2015-03-01 00:00:00