Biotechnological and molecular approaches for vanillin production: a review.

abstract：:Polygalacturonases are part of the group of enzymes involved in pectin degradation. The aim of this work was to investigate some of the factors affecting polygalacturonase production by an Aspergillus giganteus strain and to characterize this pectinolytic activity. Several carbon sources, both pure substances and natu...

journal_title：Applied biochemistry and biotechnology

authors： Pedrolli DB,Gomes E,Monti R,Carmona EC

更新日期：2008-02-01 00:00:00

abstract：:The enhancement of the biomass productivity of Escherichia coli cells harbouring the truncated 903 bp gene designated as glycoside hydrolase family 43 (GH43) from Clostridium thermocellum showing hemicellulase activity along with its further use in simultaneous saccharification and fermentation (SSF) process is descri...

journal_title：Applied biochemistry and biotechnology

authors： Das SP,Ravindran R,Ahmed S,Das D,Goyal D,Fontes CM,Goyal A

更新日期：2012-07-01 00:00:00

abstract：:Acrylamide/chitosan-based cryogel was fabricated, and a triazine dye, Reactive Green 5, was attached to the cryogel by nucleophilic substitution to build a dye affinity support for adsorption of catalase enzyme. Characterization of cryogel was performed using FTIR, SEM, EDX, BET, and swelling test. Synthesized cryogel...

journal_title：Applied biochemistry and biotechnology

authors： Yavaşer R,Karagözler AA

更新日期：2020-12-01 00:00:00

abstract：:The native Celluclast BG cellulase enzyme complex consists of different enzymes which can also degrade great substrate molecules as native celluloses. This enzyme complex has been covered by a very thin, a few nanometers thick, polymer layer, in order to improve its stability. It has been proved that the polymer layer...

journal_title：Applied biochemistry and biotechnology

authors： Hegedüs I,Hancsók J,Nagy E

更新日期：2012-11-01 00:00:00

abstract：:Endoglucanase 5 (EG5) has been isolated from the strain of E. coli TG1 harboring recombinant plasmid pCU108, which contains the cel5 gene of C. thermocellum. The enzyme has been produced with 98-fold purification and a final yield of 27% by using subsequent twofold high performance ion-exchange chromatography on Mono ...

journal_title：Applied biochemistry and biotechnology

authors： Mosolova TP,Kalyuzhnyi SV,Varfolomeyev SD,Velikodvorskaya GA

更新日期：1993-07-01 00:00:00

abstract：:To achieve targeted distribution of anticancer drugs with sustained activity, ferromagnetic ethylcellulose microcapsules containing an anticancer drug, mitomycin C (FM-MMC-mc), were prepared by a method based on phase separation principles. Two prototypes of FM-MMC-mc were made: one with the drug as the core and zinc ...

journal_title：Applied biochemistry and biotechnology

authors： Kato T,Nemoto R,Mori H,Abe R,Unno K,Goto A,Murota H,Harada M,Homma M

更新日期：1984-01-01 00:00:00

abstract：:Conjunctive administration of the tissue-type plasminogen activator (t-PA) and the urokinase-fibrinogen covalent conjugate (UK-Fbg) was studied by the example of venous thrombosis in dogs. Comparing the effect of separate use of the two components, we observed the potentiation of thrombolytic effect induced by an i.v....

journal_title：Applied biochemistry and biotechnology

authors： Maksimenko AV,Tischenko EG,Petrov AD,Petrova ML,Golubykh VL

更新日期：1996-10-01 00:00:00

abstract：:The metabolic engineering of Chlamydomonas reinhardtii, one of the fastest-growing microalgae, is a potential alternative for enhanced carotenoid productivity. CrtYB (phytoene-β-carotene synthase - PBS) gene from red yeast Xanthophyllomyces dendrorhous encodes for a bifunctional enzyme that harbours both phytoene synt...

journal_title：Applied biochemistry and biotechnology

authors： Rathod JP,Vira C,Lali AM,Prakash G

更新日期：2020-04-01 00:00:00

abstract：:L-Asparaginase (ASNase) has proved its use in medical and food industries. Sequence-based screening showed the thermophilic Streptomyces strain Streptomyces thermoluteus subsp. fuscus NBRC 14270 (14270 ASNase) to positive against predicted ASNase primary sequences. The 14270 ASNase gene and four L-asparaginase genes f...

journal_title：Applied biochemistry and biotechnology

authors： Hatanaka T,Usuki H,Arima J,Uesugi Y,Yamamoto Y,Kumagai Y,Yamasato A,Mukaihara T

更新日期：2011-04-01 00:00:00

abstract：:S-Adenosylhomocysteine hydrolase (SAHase) encoded by sahase gene is a determinant when catalyzing the reversible conversion of adenosine and homocysteine to S-adenosylhomocysteine in most living organisms. The sahase gene was isolated from the genome of the highly thermostable anaerobic bacteria Thermotoga maritima, a...

journal_title：Applied biochemistry and biotechnology

authors： Lozada-Ramírez JD,Sánchez-Ferrer A,García-Carmona F

更新日期：2013-06-01 00:00:00

abstract：:MicroRNAs are small non-coding RNAs which play significant role in RNA interference. The present work deals with mining of the conserved miRNA and their target genes from the contigs, ESTs, and BAC end sequences of commercially important catfish, Clarias batrachus, from India. A total of 138, 1 and 1 conserved pre-miR...

journal_title：Applied biochemistry and biotechnology

authors： Agarwal S,Nagpure NS,Srivastava P,Kumar R,Pandey M,Srivastava S,Jena JK,Das P,Kushwaha B

更新日期：2017-07-01 00:00:00

abstract：:Application of statistical experimental designs for optimization of fermentation parameters to enhance ethanol production, which is an economical and renewable energy source using Saccharomyces cerevisiae NCIM 3090 from palmyra jaggery, was studied in a batch fermentor. Using Plackett-Burman design, impeller speed, co...

journal_title：Applied biochemistry and biotechnology

authors： Bandaru VV,Bandaru SR,Somalanka SR,Mendu DR,Imandi SB,Bejawada SR,Medicherla NR,Devarajan T,Karothi J,Chityala A

更新日期：2007-12-01 00:00:00

abstract：:Lysozymes are known as ubiquitously distributed immune effectors with hydrolytic activity against peptidoglycan, the major bacterial cell wall polymer, to trigger cell lysis. In the present study, the full-length cDNA sequence of a novel sea urchin Strongylocentrotus purpuratus invertebrate-type lysozyme (sp-iLys) was...

journal_title：Applied biochemistry and biotechnology

authors： Huang P,Shi J,Sun Q,Dong X,Zhang N

更新日期：2018-10-01 00:00:00

abstract：:A combined process, de-algination followed by enzymatic saccharification, was designed to produce alginate and glucose from Saccharina japonica consecutively. The process conditions of de-algination were optimized separately for each stage of acidification and alkaline extraction. Collectively, the de-algination yield...

journal_title：Applied biochemistry and biotechnology

authors： Ryu HJ,Oh KK

更新日期：2017-05-01 00:00:00

abstract：CONCLUSION:Immobilization of the thermophilic lactase causes a decrease in the formation of intermediary products. This means that partially hydrolyzed lactose syrups contain less of these intermediary products when the immobilized thermophilic lactase is used in the hydrolysis process than when a soluble lactase is us...

journal_title：Applied biochemistry and biotechnology

authors： Rugh S

更新日期：1982-01-01 00:00:00

abstract：:Paraoxonase 1 (PON1: EC 3.1.8.1) is a calcium-dependent enzyme associated with high-density lipoproteins (HDLs) and has a protective effect against oxidation of low-density lipoproteins (LDLs) in mammals. PON1 is the best-studied member of a family of enzymes called serum paraoxonases, or PONs, identified in mammals a...

journal_title：Applied biochemistry and biotechnology

authors： Erzengin M,Demir D,Arslan M,Sinan S

更新日期：2014-08-01 00:00:00

abstract：:A simple, sensitive aptamer-based biosensor for the detection of phenylalanine is developed using the electrochemical transduction method. For this proposed aptasensor, a 5-thiol-terminated aptamer is covalently attached onto a gold electrode. At the first time, the electrode was evaluated as an electrochemical aptase...

journal_title：Applied biochemistry and biotechnology

authors： Omidinia E,Shadjou N,Hasanzadeh M

更新日期：2014-02-01 00:00:00

abstract：:In this study, an easily detectable method was employed for screening laccase-producing microorganisms by using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) as laccase secretion indicator. A novel laccase-producing strain was isolated and identified as Shiraia bambusicola Henn. strain GZ11K2 according to the mor...

journal_title：Applied biochemistry and biotechnology

authors： Du W,Sun C,Yu J,Liang J,Liang Z,Han Y,Zou X

更新日期：2012-12-01 00:00:00

abstract：:A spectrophotometric method of measuring oxygenase activity in cell extracts or in zymograms was developed. It is an easy and cheap method that allows spectrophotometric measurement of activity by a colored reaction and reveals activity bands in a polyacrylamide gel electrophoresis (PAGE) gel as brown bands. To prove ...

journal_title：Applied biochemistry and biotechnology

authors： Zazueta-Sandoval R,Novoa VZ,Jiménez HS,Ortiz RC

更新日期：2003-04-01 00:00:00

abstract：:Agrobacterium isolated from soil samples produced two extracellular polysaccharides: succinoglycan, an acidic soluble polymer, and curdlan gum, a neutral, insoluble polymer. Maize glucose, cassava glucose, and maize maltose were used in fermentation medium to produce insoluble polysaccharide. Two Agrobacterium sp. str...

journal_title：Applied biochemistry and biotechnology

authors： Portilho M,Matioli G,Zanin GM,de Moraes FF,Scamparini AR

更新日期：2006-03-01 00:00:00

abstract：:The capacity for anaerobic decolorization of a sulfonated azo dye, Congo Red, by a strain of a sulfate-reducing bacterium was evaluated. After optimizing the growth rate of the bacteria on a simple carbon source and terminal electron acceptor pair, lactate and sulfate, respectively, the effect of the dye concentration...

journal_title：Applied biochemistry and biotechnology

authors： Diniz PE,Lopes AT,Lino AR,Serralheiro ML

更新日期：2002-03-01 00:00:00

abstract：:EDTA, the target compound of this study from the effluent of secondary biotreatment units, can be biodegraded by special microorganisms. So far, there are three species of microorganisms--Agrobacterium, Gram-negative BNCI, and DSM9103--that can degrade EDTA and are published in the literature. We have successfully iso...

journal_title：Applied biochemistry and biotechnology

authors： Fang HY,Chen SC,Chen SL

更新日期：2003-11-01 00:00:00

abstract：:Beta-xylosidase recovery by micelles using cetyl trimethyl ammonium bromide (CTAB) cationic surfactant was verified under different experimental conditions. A 2(5-1) fractional factorial design with center points was employed to verify the influence of the following factors on enzyme extraction: pH (x1), CTAB concentr...

journal_title：Applied biochemistry and biotechnology

authors： Hasmann FA,Pessoa A Jr,Roberto IC

更新日期：2001-04-01 00:00:00

abstract：:Several recombinant derivatives of serine protease inhibitor called silk protease inhibitor 2 (SPI2), which is a silk component in Galleria mellonella (Lepidoptera, Insecta), were prepared in the expression vector Pichia pastoris. Both the native and the recombinant protease inhibitors were highly active against subti...

journal_title：Applied biochemistry and biotechnology

authors： Kodrík D,Kludkiewicz B,Navrátil O,Skoková Habuštová O,Horáčková V,Svobodová Z,Vinokurov KS,Sehnal F

更新日期：2013-09-01 00:00:00

abstract：:Bacillus amyloliquefaciens LL3 is a glutamate-independent poly-γ-glutamic acid (γ-PGA) producing strain which consists of a circular chromosome (3,995,227 bp) and an endogenous plasmid pMC1 (6,758 bp). The study of the function of native plasmid and the genome-size reduction of the B. amyloliquefaciens LL3 strain requ...

journal_title：Applied biochemistry and biotechnology

authors： Feng J,Gu Y,Wang J,Song C,Yang C,Xie H,Zhang W,Wang S

更新日期：2013-09-01 00:00:00

abstract：:An extracellular lipase was purified from the fermentation broth of Penicillium expansum PED-03 by DEAE-Sepharose chromatography, followed by sephacryl S-200 chromatography. The enzyme was purified 81.8-fold with 19.8% recovery and a specific activity of 85.94 U/mg. The molecular weight of the homogeneous enzyme was a...

journal_title：Applied biochemistry and biotechnology

authors： Lianghua T,Liming X,Min S,Huaying G

更新日期：2007-08-01 00:00:00

abstract：:The high cost of nicotinamide cofactors requires that they be regenerated in situ when used in preparative enzymatic synthesis. Numerous strategies have been tested for in situ regeneration of reduced and oxidized cofactors. Regeneration of reduced cofactors is relatively straightforward; regeneration of oxidized cofa...

journal_title：Applied biochemistry and biotechnology

authors： Chenault HK,Whitesides GM

更新日期：1987-03-01 00:00:00

abstract：:As a part of our study on microbial heterogeneity in subsurface environments, we have examined the microbial respiration of sediment samples obtained from a coastal site near Oyster, VA. The sediments at the site are unconsolidated, fine to coarse beach sand and gravel. A Columbus Instruments Micro-Oxymax Respirometer...

journal_title：Applied biochemistry and biotechnology

authors： Chapatwala KD,Babu GR,Vijaya OK,Armstead E,Palumbo AV,Zhang C,Phelps TJ

更新日期：1996-04-01 00:00:00

abstract：:The enzyme cellobiase from Novo was immobilized in controlled pore silica particles by covalent binding with the silane-glutaraldehyde method with protein and activity yields of 67 and 13.7%, respectively. The activity of the free enzyme (FE) and immobilized enzyme (IE) was determined with 2 g/L of cellobiose, from 40...

journal_title：Applied biochemistry and biotechnology

authors： Calsavara LP,De Moraes FF,Zanin GM

更新日期：2001-04-01 00:00:00

abstract：:The three-dimensional distribution (x, y, and z) of ultrasound-induced microalgal cell disruption in a sonochemical reactor was predicted by solving the Helmholtz equation using a three-dimensional acoustic module in the COMSOL Multiphysics software. The simulated local ultrasound pressure at any given location (x, y,...

journal_title：Applied biochemistry and biotechnology

authors： Wang M,Yuan W,Hale A

更新日期：2016-03-01 00:00:00