Abstract:
:Field contamination with pesticide mixtures of organophosphates (OPs) and organochlorines (OCs) is becoming global issues to be solved urgently. The strategy of utilizing engineered microorganisms that have an ability to simultaneously degrade OPs and OCs has increasingly received great interest. In this work, an OP degradation gene (mpd) and an OC degradation gene (linA) were simultaneously introduced into Escherichia coli by using two compatible plasmids, resulting in strains with both OP degradation and OC degradation capabilities. To overcome the potential substrate uptake limitation, MPH was displayed on the cell surface of Escherichia coli using the N- and C-terminal domains of ice nucleation protein (INPNC) as an anchoring motif. The surface localization of INPNC-MPH was verified by cell fractionation, Western blot, proteinase accessibility, and immunofluorescence microscopy. Furthermore, both LinA and green fluorescent protein (GFP) were functionally co-expressed in the MPH-displaying Escherichia coli. The engineered Escherichia coli degraded OPs as well as OCs rapidly, and it can be easily monitored by GFP fluorescence.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Yang J,Liu R,Song W,Yang Y,Cui F,Qiao Cdoi
10.1007/s12010-011-9450-5subject
Has Abstractpub_date
2012-02-01 00:00:00pages
590-8issue
3eissn
0273-2289issn
1559-0291journal_volume
166pub_type
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