Cloning, expression, and characterization of a milk-clotting aspartic protease gene (Po-Asp) from Pleurotus ostreatus.

abstract：:The use of probiotics, alone or in interaction with bile acids, is a modern strategy in the prevention and treatment of hypercholesterolemia. Numerous mechanisms for hypocholesterolemic effect of probiotics have been hypothesized, based mostly on in vitro evidence. Interaction with bile acids through reaction of decon...

journal_title：Applied biochemistry and biotechnology

authors： Pavlović N,Stankov K,Mikov M

更新日期：2012-12-01 00:00:00

abstract：:Amyloglucosidase from Novo (Copenhagen, Denmark) was immobilized in controlled pore silica particles with the silane-glutaraldehyde covalent method. Thermal stability of the free and immobilized enzyme (IE) was determined with 30% (w/v) alpha-amylase liquefied cassava starch, pH 4.5, temperatures from 35 to 75 degrees...

journal_title：Applied biochemistry and biotechnology

authors： Zanin GM,De Moraes FF

更新日期：1998-04-01 00:00:00

abstract：:Enzyme-linked immunosorbent assay (ELISA), horseradish peroxidase (HRP)-catalyzed fluorescent reaction, and oxalate chemiluminescence analysis have been combined to develop a highly sensitive, simple, and rapid method for analysis of bovine serum albumin (BSA) based on a pair of specific monoclonal antibodies in vacci...

journal_title：Applied biochemistry and biotechnology

authors： Xue P,Zhang K,Zhang Z,Li Y,Liu F,Sun Y,Zhang X,Song C,Fu A,Jin B,Yang K

更新日期：2012-03-01 00:00:00

abstract：:A green synthesis of iron oxide nanoparticles (FeNPs) was developed using Murraya koenigii leaf extract as reducing and stabilizing agent. UV-vis spectra show that the absorption band centred at a wavelength of 277 nm which corresponds to the surface plasmon resonances of synthesized FeNPs. Fourier transform infrared ...

journal_title：Applied biochemistry and biotechnology

authors： Mohanraj S,Kodhaiyolii S,Rengasamy M,Pugalenthi V

更新日期：2014-05-01 00:00:00

abstract：:Phosphoinositide 3-kinase (PI3K) enzyme plays a vital role in the insulin signaling pathway as well as in other pathways that are involved in the growth, migration, and survival of cells. In the insulin signaling pathway, PI3K proteins that include p50α, p85α, p85β, p55γ, p110α, p110β, and p110γ are associated with th...

journal_title：Applied biochemistry and biotechnology

authors： Chakraborty C,Doss CG,Bhatia R,Agoramoorthy G

更新日期：2015-04-01 00:00:00

abstract：:Recently, poly(3-hydroxybutyrate) (PHB) has been found in a few thermophilic strains where several advantages can be gained from running fermentation at high temperatures. Caldimonas manganoxidans, a thermophilic gram-negative bacterium, was investigated for the feasibility as a PHB-producing strain. It is suggested t...

journal_title：Applied biochemistry and biotechnology

authors： Hsiao LJ,Lin JH,Sankatumvong P,Wu TM,Li SY

更新日期：2016-11-01 00:00:00

abstract：:Molecular chaperones and protein folding factors of bacterial periplasmic space play important roles in assisting disulfide bond formation and proper protein folding. In this study, effects of disulfide bond protein (Dsb) families were investigated on assembly of 3F3 Fab, an antibody inhibitor targeting matrix metallo...

journal_title：Applied biochemistry and biotechnology

authors： Rodriguez C,Nam DH,Kruchowy E,Ge X

更新日期：2017-10-01 00:00:00

abstract：:Foot and mouth disease virus, (FMDV) from a crude cell lysate was purified in a single step by affinity chromatography with heparin as a ligand. The virus eluted from an Heparin-Ultrogel A4R column at 1M sodium chloride in 10 mM sodium phosphate buffer, pH 7.0, while most cell protein and albumin did so at lower conce...

journal_title：Applied biochemistry and biotechnology

authors： Navarro del Cañizo AA,Mazza M,Bellinzoni R,Cascone O

更新日期：1996-12-01 00:00:00

abstract：:Methanol, acetone and diethyl ether extracts of Alpinia galanga have been evaluated against pathogens viz. Bacillus subtilis MTCC 2391, Enterobacter aerogene, Enterobacter cloacae, Enterococcus faecalis, Escherichia coli MTCC 1563, Klebsiella pneumoniae, Pseudomonas aeruginosa MTCC 6642, Salmonella typhimurium, Staphy...

journal_title：Applied biochemistry and biotechnology

authors： Rao K,Ch B,Narasu LM,Giri A

更新日期：2010-10-01 00:00:00

abstract：:The transesterification of soybean lecithin with ethyl esters of polyunsaturated fatty acids (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) using immobilized lipase from Rhizomucor miehei was tested in the presence or absence of organic solvent (hexane) and additives (urea with Ca(2+) or Mg(2+)). The rea...

journal_title：Applied biochemistry and biotechnology

authors： Marsaoui N,Naghmouchi K,Baah J,Raies A,Laplante S

更新日期：2015-06-01 00:00:00

abstract：:An integrated wet-milling and alkali pretreatment was applied to corn stover prior to enzymatic hydrolysis. The effects of NaOH concentration in the pretreatment on crystalline structure, chemical composition, and reducing-sugar yield of corn stover were investigated, and the mechanism of increasing reducing-sugar yie...

journal_title：Applied biochemistry and biotechnology

authors： He X,Miao Y,Jiang X,Xu Z,Ouyang P

更新日期：2010-04-01 00:00:00

abstract：:To meet the increasing demands of sophorolipids as biosurfactants and bioactive compounds, it is necessary to obtain higher and more specific sophorolipid-producing strains. One sophorolipid-producing strain, Wickerhamiella domercqiae var. sophorolipid CGMCC 1576 (Y(2A)), was mutated by low-energy nitrogen ion beam im...

journal_title：Applied biochemistry and biotechnology

authors： Li H,Ma X,Shao L,Shen J,Song X

更新日期：2012-06-01 00:00:00

abstract：:The enzymatic hydrolysates of the lipid-extracted microalgal biomass residues (LMBRs) from biodiesel production were evaluated as nutritional sources for the mixotrophic growth of Chlorella vulgaris and lipid production at different temperature levels and substrate concentrations. Both parameters had a significant eff...

journal_title：Applied biochemistry and biotechnology

authors： Ma X,Zheng H,Huang H,Liu Y,Ruan R

更新日期：2014-10-01 00:00:00

abstract：:Here, we demonstrate the micropropagation protocol of Argyrolobium roseum (Camb.), an endangered herb exhibiting anti-diabetic and immune-suppressant properties, and antioxidant enzymes pattern is evaluated. Maximum callogenic response (60 %) was observed from leaf explant at 1.0 mg L(-1) 1-nephthalene acetic acid (NA...

journal_title：Applied biochemistry and biotechnology

authors： Habib D,Chaudhary MF,Zia M

更新日期：2014-01-01 00:00:00

abstract：:The methods for increasing the production of ergothioneine (ERG) were investigated by using the mycelial culture of several mushroom species, primarily Ganoderma neo-japonicum. We first found that ERG was accumulated at the different levels in mycelia and fruiting bodies, respectively, depending on the mushroom specie...

journal_title：Applied biochemistry and biotechnology

authors： Lee WY,Park EJ,Ahn JK

更新日期：2009-07-01 00:00:00

abstract：:Cofermentation of glucose, xylose, and arabinose is critical for complete bioconversion of lignocellulosic biomass, such as agricultural residues and herbaceous energy crops, to ethanol. We have previously developed a plasmid-bearing strain of Zymomonas mobilis (206C[pZB301]) capable of cofermenting glucose, xylose, a...

journal_title：Applied biochemistry and biotechnology

authors： Mohagheghi A,Evans K,Chou YC,Zhang M

更新日期：2002-04-01 00:00:00

abstract：:In a previous study, a recombination system based on the alpha complementation of cre recombinase and protein transduction was established. This system relied on the transient expression of the inactive, self-excisable C-terminal (beta) and the transduction of the N-terminal (alpha) cre fragments to cells as a purifie...

journal_title：Applied biochemistry and biotechnology

authors： Seidi A,Mie M,Kobatake E

更新日期：2009-08-01 00:00:00

abstract：:The rheological behavior of a sulfated galactan extracted from Halymenia durvillei, a red seaweed collected in the coastal waters of a small island of Madagascar (Nosy-be in Indian Ocean), was investigated in dilute and semi-dilute solutions. In dilute solution with NaCl at 0.3 M, the polysaccharide adopted a coil con...

journal_title：Applied biochemistry and biotechnology

authors： Fenoradosoa TA,Laroche C,Delattre C,Dulong V,Le Cerf D,Picton L,Michaud P

更新日期：2012-07-01 00:00:00

abstract：:A new cellulase producer strain of Penicillium digitatum (RV 06) was previously obtained from rotten maize grains. This work aim was to optimize the production and characterize this microorganism produced cellulase. A CMCase maximum production (1.6 U/mL) was obtained in stationary liquid culture, with an initial pH of...

journal_title：Applied biochemistry and biotechnology

authors： Dos Santos FC,de Oliveira MAS,Seixas FAV,Barbosa-Tessmann IP

更新日期：2020-09-01 00:00:00

abstract：:A high cellobiohydrolase (CBH)-producing strain was isolated and identified as Penicillium purpurogenum KJS506 according to the morphology and comparison of internal transcribed spacer rDNA gene sequence. When rice straw and corn steep powder were used as carbon and nitrogen sources, respectively, a maximum CBH activi...

journal_title：Applied biochemistry and biotechnology

authors： Lee KM,Joo AR,Jeya M,Lee KM,Moon HJ,Lee JK

更新日期：2011-01-01 00:00:00

abstract：:Apoptotic cell death is a fundamental process in the development and physiological homeostasis of multicellular organisms. It is associated with control of cell numbers in tissues and organs during development, with cell turnover, and with response to infection. Molecules that trigger this process in continuously prol...

journal_title：Applied biochemistry and biotechnology

authors： Das MK,Sharma RS,Mishra V

更新日期：2012-03-01 00:00:00

abstract：:This investigation utilized sugarcane bagasse aqueous extract (SBAE), a nontoxic, cost-effective medium to boost triacylglycerol (TAG) accumulation in novel fresh water microalgal isolate Scenedesmus sp. IITRIND2. Maximum lipid productivity of 112 ± 5.2 mg/L/day was recorded in microalgae grown in SBAE compared to mod...

journal_title：Applied biochemistry and biotechnology

authors： Arora N,Patel A,Pruthi PA,Pruthi V

更新日期：2016-09-01 00:00:00

abstract：:This research aimed at developing and designing a slow and targeted delivery of Amphotericin B (AmB) antibiotic by placing three types of shells containing different ratios of biodegradable and biocompatible polymers poly (D, L-lactide)-co-(glycolide) (PLGA), polyethylene glycol (PEG), and polyvinyl pyrrolidone (PVP) ...

journal_title：Applied biochemistry and biotechnology

authors： Sadat Akhavi S,Moradi Dehaghi S

更新日期：2020-06-01 00:00:00

abstract：:Lipase-catalyzed esterification of selected phenolic acids with linolenyl alcohols was investigated in selected organic solvent media. The enzyme activity for the esterification of dihydrocaffeic acid with linolenyl alcohol in solvent mixtures of hexane/2-butanone of 75:25 (v/v) and 65:35 (v/v) was 0.88 and 0.47 micro...

journal_title：Applied biochemistry and biotechnology

authors： Sabally K,Karboune S,Yeboah FK,Kermasha S

更新日期：2005-10-01 00:00:00

abstract：:Forty-four endophytic fungal isolates obtained from marine sponge, Hyrtios erectus, were evaluated and screened for their hydrolase activities. Most of the isolates were found to be prolific producers of hydrolytic enzymes. Only 11 isolates exhibited maximum cellular contents of lipids, rhamnolipids, and protein in th...

journal_title：Applied biochemistry and biotechnology

authors： El-Gendy MMAA,Yahya SMM,Hamed AR,Soltan MM,El-Bondkly AMA

更新日期：2018-07-01 00:00:00

abstract：:A novel β-1,3-glucanase gene, designated Ccglu17A, was cloned from the biological control fungus Chaetomium cupreum Ame. Its 1626-bp open reading frame encoded 541 amino acids. The corresponding amino acid sequence showed highest identity (67 %) with a glycoside hydrolase family 17 β-1,3-glucanase from Chaetomium glob...

journal_title：Applied biochemistry and biotechnology

authors： Jiang C,Song J,Cong H,Zhang J,Yang Q

更新日期：2017-05-01 00:00:00

abstract：:In order to detect low levels of enzyme activity, specifically glucose oxidase, in biological samples, an immunoenzymatic assay was developed since currently available methods could not be used because of either their lack of sensitivity or the conditions prevailing in our samples: turbidity of the medium, presence of...

journal_title：Applied biochemistry and biotechnology

authors： de Bellefontaine C,Josse F,Domurado M,Domurado D

更新日期：1994-08-01 00:00:00

abstract：:To facilitate structural studies of receptor-interacting protein 3 (RIP3), we developed a large-scale expression system of a glutathione-S-transferase (GST) fused with an 82 amino acid RIP3 protein in Escherichia coli. RIP3 truncation was subcloned into the pGEX-4T-1 vector and overexpressed in BL21(DE3)RIL cells. The...

journal_title：Applied biochemistry and biotechnology

authors： Jeong MS,Park JS,Jang SB

更新日期：2007-05-01 00:00:00

abstract：:Dissolved oxygen (DO) is an imperative parameter of the activated sludge process (ASP) for wastewater bioremediation. The effect of DO on microbial communities and corresponding metabolic functions in wastewater bioremediation was investigated using next-generation analysis techniques in this study. Illumina-based who...

journal_title：Applied biochemistry and biotechnology

authors： Yadav TC,Jadeja NB,Kapley A

更新日期：2020-10-01 00:00:00

abstract：:Drinking water distribution systems are composed of various pipe materials and may harbor biofilms even in the continuous presence of disinfectants. Biofilms formation on five pipe materials (copper (Cu), polyethylene (PE), stainless steel (STS), cast iron (CI), and concrete-coated polycarbonate (CP)) within drinking ...

journal_title：Applied biochemistry and biotechnology

authors： Zhu Z,Wu C,Zhong D,Yuan Y,Shan L,Zhang J

更新日期：2014-07-01 00:00:00