Recombination system based on cre alpha complementation and leucine zipper fusions.


:In a previous study, a recombination system based on the alpha complementation of cre recombinase and protein transduction was established. This system relied on the transient expression of the inactive, self-excisable C-terminal (beta) and the transduction of the N-terminal (alpha) cre fragments to cells as a purified protein. This recombination system potentially results in a less invasive and more controllable cre recombination in mammalian cells. In this study, we have employed a more efficient complementation triggering sequence using more than only the overlapping amino acids to help the alpha and beta fragments reassociate. In order to increase the association efficiency of the complementing fragments of cre recombinase, we chose to use a fusion of cre fragments to a self-heterodimerizing pair of proteins to trigger their binding and thus increase the efficiency of the restored enzymatic activity. For this purpose, the leucine zipper motifs (bJun and bFos) of the AP-1 transcription were fused to cre fragments (alpha and beta, respectively). This resulted in an increased reassociation efficiency of the fragments and a two times more efficient recombination system compared with the previous study.


Seidi A,Mie M,Kobatake E




Has Abstract


2009-08-01 00:00:00












  • Gastrodin production from p-2-hydroxybenzyl alcohol through biotransformation by cultured cells of Aspergillus foetidus and Penicillium cyclopium.

    abstract::The objective of this work was to take advantage of the resting cells of suitable fungus as an in vitro model to prepare gastrodin from p-2-hydroxybenzyl alcohol (HBA), which mainly exists in the metabolites of the plant Gastrodia elata Blume. The one-step biotransformation of HBA into gastrodin was examined with the ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Fan L,Dong Y,Xu T,Zhang H,Chen Q

    更新日期:2013-05-01 00:00:00

  • Simultaneous saccharification and fermentation of steam-pretreated spruce to ethanol.

    abstract::Ethanol production was studied in simultaneous saccharification and fermentation (SSF) of steam-pretreated spruce at 42 degrees C, using a thermotolerant yeast. Three yeast strains of Kluyveromyces marxianus were compared in test fermentations. SSF experiments were performed with the best of these on 5% (w/w) of subst...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Bollók M,Réczey K,Zacchi G

    更新日期:2000-04-01 00:00:00

  • Engineering Pichia pastoris for Efficient Production of a Novel Bifunctional Strongylocentrotus purpuratus Invertebrate-Type Lysozyme.

    abstract::Lysozymes are known as ubiquitously distributed immune effectors with hydrolytic activity against peptidoglycan, the major bacterial cell wall polymer, to trigger cell lysis. In the present study, the full-length cDNA sequence of a novel sea urchin Strongylocentrotus purpuratus invertebrate-type lysozyme (sp-iLys) was...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Huang P,Shi J,Sun Q,Dong X,Zhang N

    更新日期:2018-10-01 00:00:00

  • New Insights on Coffea miRNAs: Features and Evolutionary Conservation.

    abstract::Small RNAs influence the gene expression at the post-transcriptional level by guiding messenger RNA (mRNA) cleavage, translational repression, and chromatin modifications. In addition to model plants, the microRNAs (miRNAs) have been identified in different crop species. In this work, we developed a specific pipeline ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Chaves SS,Fernandes-Brum CN,Silva GF,Ferrara-Barbosa BC,Paiva LV,Nogueira FT,Cardoso TC,Amaral LR,de Souza Gomes M,Chalfun-Junior A

    更新日期:2015-10-01 00:00:00

  • Enhanced secondary metabolite biosynthesis by elicitation in transformed plant root system: effect of abiotic elicitors.

    abstract::Plants generally produce secondary metabolites in nature as a defense mechanism against pathogenic and insect attack. In this study, we applied several abiotic elicitors in order to enhance growth and ginseng saponin biosynthesis in the hairy roots of Panax ginseng. Generally, elicitor treatments were found to inhibit...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Jeong GT,Park DH

    更新日期:2006-04-01 00:00:00

  • Potential Application and Bactericidal Mechanism of Lactic Acid-Hydrogen Peroxide Consortium.

    abstract::It has been found that lactic acid and hydrogen peroxide (H2O2) displayed co-operatively enhanced killing activity to pathogens. The synergistic effect was investigated with using several microbe species, suggesting that low concentration of lactic acid and H2O2 could kill both Gram-negative and Gram-positive bacteria...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Zhang C,Zhang S,Liu W,Guo T,Gu R,Kong J

    更新日期:2019-11-01 00:00:00

  • Improvement of zinc bioaccumulation and biomass yield in the mycelia and fruiting bodies of Pleurotus florida cultured on liquid media.

    abstract::The effect of different concentrations of zinc on the bioaccumulation of zinc and biomass yield in both mycelium and fruiting body of Pleurotus florida cultivated in liquid medium was studied. The results showed that the optimum yield of mycelia (11.33 ± 0.44 g/L) and fruiting bodies (7.70 ± 0.19 g/L) dry biomass was ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Poursaeid N,Azadbakht A,Balali GR

    更新日期:2015-04-01 00:00:00

  • Statistical optimization of alpha-amylase production with immobilized cells of Streptomyces erumpens MTCC 7317 in Luffa cylindrica L. sponge discs.

    abstract::The purpose of this investigation was to study the effect of Streptomyces erumpens cells immobilized in various matrices, i.e., agar-agar, polyacrylamide, and luffa (Luffa cylindrica L.) sponge for production of alpha-amylase. Luffa sponge was found to be 21% and 51% more effective in enzyme yield than agar-agar and p...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Kar S,Swain MR,Ray RC

    更新日期:2009-02-01 00:00:00

  • Characterization of a family B DNA polymerase from the hyperthermophilic crenarchaeon Ignicoccus hospitalis KIN4/I and its application to PCR.

    abstract::A family B DNA polymerase gene from the hyperthermophilic crenarchaeon Ignicoccus hospitalis KIN4/I was highly expressed under the control of T7lac promoter of pET-28ARG in Escherichia coli BL21-CodonPlus(DE3)-RIL cells. The produced I. hospitalis (Iho) DNA polymerase was purified by heat treatment followed by HisTrap...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Seo KJ,Cho SS,Ppyun HW,Youn MH,Kim SH,Seo BS,Kwon ST

    更新日期:2014-07-01 00:00:00

  • Application of a chitosan-immobilized Talaromyces thermophilus lipase to a batch biodiesel production from waste frying oils.

    abstract::Waste frying oil, which not only harms people's health but also causes environmental pollution, can be a good alternative to partially substitute petroleum diesel through transesterification reaction. This oil contained 8.8 % of free fatty acids, which cause a problem in a base-catalyzed process. In this study, synthe...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Romdhane IB,Romdhane ZB,Bouzid M,Gargouri A,Belghith H

    更新日期:2013-12-01 00:00:00

  • Real-Time and Online Monitoring of Glucose Contents by Using Molecular Imprinted Polymer-Based IDEs Sensor.

    abstract::A highly sensitive, selective, reversible, and reusable glucose sensor is developed by using molecularly imprinted polymer-based artificial receptors onto interdigital transducer. Sensor receptors were synthesized through bulk imprinting technology by using styrene as monomer, ethylene glycol dimethacrylate (EGDMA) as...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Asghar N,Mustafa G,Yasinzai M,Al-Soud YA,Lieberzeit PA,Latif U

    更新日期:2019-12-01 00:00:00

  • Study on the production of a xylanolytic complex from Penicillium canescens 10-10c.

    abstract::We screened about a hundred microorganisms (including unidentified yeasts, fungi, and bacteria) for their ability to produce xylanolytic enzymes. About 40 of them were hemicellulolytic; among these, we selected Penicillium canescens 10-10c for detailed study because of its ability to produce an interesting enzymatic c...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Gaspar A,Cosson T,Roques C,Thonart P

    更新日期:1997-07-01 00:00:00

  • Protein Production Through Microbial Conversion of Rice Straw by Multi-Strain Fermentation.

    abstract::Multi-strain mixed fermentation can provide a relatively complete lignocellulosic enzyme system compared with single-strain fermentation. This study was firstly to screen strains which have a strong ability to hydrolyse rice straw (RS) enzymatically and enrich true protein (TP). Then, the conditions in the process of ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Jia J,Chen H,Wu B,Cui F,Fang H,Wang H,Ni Z

    更新日期:2019-01-01 00:00:00

  • Mutagenesis for Improvement of Activity and Stability of Prolyl Aminopeptidase from Aspergillus oryzae.

    abstract::In this study, the prokaryotic expression system of Escherichia coli was used to modify prolyl aminopeptidase derived from Aspergillus oryzae JN-412 (AoPAP) via random mutagenesis and site-directed saturation mutagenesis. A random mutant library with a capacity of approximately 3000 mutants was compiled using error-pr...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Liu D,Zhang D,Huang Q,Gu L,Zhou N,Tian Y

    更新日期:2020-08-01 00:00:00

  • Phytodegradation potential of Erythrina crista-galli L., Fabaceae, in petroleum-contaminated soil.

    abstract::This work aimed at investigating both the tolerance and the phytodegradation potential of Erythrina crista-galli L. in petroleum-contaminated soil. It consisted in analyzing E. crista-galli germination, surviving, growth, and development when cultivated at different contaminant concentrations and pollutant degradation...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: de Farias V,Maranho LT,de Vasconcelos EC,da Silva Carvalho Filho MA,Lacerda LG,Azevedo JA,Pandey A,Soccol CR

    更新日期:2009-04-01 00:00:00

  • Venous thrombolysis by tissue-type plasminogen activator in conjunction with the urokinase-fibrinogen covalent conjugate. Effects of potentiation and faster action in dogs.

    abstract::Conjunctive administration of the tissue-type plasminogen activator (t-PA) and the urokinase-fibrinogen covalent conjugate (UK-Fbg) was studied by the example of venous thrombosis in dogs. Comparing the effect of separate use of the two components, we observed the potentiation of thrombolytic effect induced by an i.v....

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Maksimenko AV,Tischenko EG,Petrov AD,Petrova ML,Golubykh VL

    更新日期:1996-10-01 00:00:00

  • Impact of targeted specific antibiotic delivery for gut microbiota modulation on high-fructose-fed rats.

    abstract::The objective of present investigation was to study the effect of gut microbiota alteration by oral administration of targeted delivery of pH sensitive cefdinir microspheres to high-fructose-fed (HFD) rats. Rats were fed with a high-fructose diet with or without cefdinir microsphere administration for 30 days. The fec...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Jena PK,Singh S,Prajapati B,Nareshkumar G,Mehta T,Seshadri S

    更新日期:2014-04-01 00:00:00

  • Influence of Altered NADH Metabolic Pathway on the Respiratory-deficient Mutant of Rhizopus oryzae and its L-lactate Production.

    abstract::Respiratory-deficient mutants of Rhizopus oryzae (R. oryzae) AS 3.3461 were acquired by ultraviolet (UV) irradiation to investigate changes in intracellular NADH metabolic pathway and its influence on the fermentation characteristics of the strain. Compared with R. oryzae AS 3.3461, the intracellular ATP level of the ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Shu C,Guo C,Luo S,Jiang S,Zheng Z

    更新日期:2015-08-01 00:00:00

  • Biochemical, immunological and kinetic characterisation of thiol protease inhibitor (cystatin) from liver.

    abstract::Regulation of the cysteine protease activity is imperative for proper functioning of the various organ systems. Elevated activities of cysteine proteinases due to impaired regulation by the endogenous cysteine proteinase inhibitors (cystatins) have been linked to liver malignancies. To gain an insight into these regul...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Shah A,Priyadarshini M,Khan MS,Aatif M,Amin F,Bano B

    更新日期:2013-10-01 00:00:00

  • Studies on productivity and characterization of polygalacturonase from Aspergillus giganteus submerged culture using citrus pectin and orange waste.

    abstract::Polygalacturonases are part of the group of enzymes involved in pectin degradation. The aim of this work was to investigate some of the factors affecting polygalacturonase production by an Aspergillus giganteus strain and to characterize this pectinolytic activity. Several carbon sources, both pure substances and natu...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Pedrolli DB,Gomes E,Monti R,Carmona EC

    更新日期:2008-02-01 00:00:00

  • Site-Directed Mutagenesis of Cytochrome P450 2D6 and 2C19 Enzymes: Expression and Spectral Characterization of Naturally Occurring Allelic Variants.

    abstract::Genetic polymorphism of the cytochrome P450 (CYP) genes particularly affects CYP2D6 and CYP2C19 to a functionally relevant extent, and it is therefore crucial to elucidate the enzyme kinetic and molecular basis for altered catalytic activity of these allelic variants. This study explored the expression and function of...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Dong AN,Pan Y,Palanisamy UD,Yiap BC,Ahemad N,Ong CE

    更新日期:2018-09-01 00:00:00

  • Role of selenium supplementation and heat stress on trehalose and glutathione content in Saccharomyces cerevisiae.

    abstract::The role of selenium (Se) supplementation on glutathione, a potent intracellular redox buffer, and trehalose, a well-known stress protectant molecule, was studied. The amount of glutathione decreased significantly while that of trehalose showed a minor decrease in the cells grown in Se-supplemented medium. After heat ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Sharma SC,Anand MS

    更新日期:2006-04-01 00:00:00

  • Bioconversion of sodium dodecyl sulphate to rhamnolipid by Pseudomonas aeruginosa: a novel and cost-effective production strategy.

    abstract::The utility of rhamnolipids in industry is currently limited due to the high constraints in its economic production. In this scenario, the novel utility of sodium dodecyl sulphate (SDS) as carbon source could serve as promising cost-effective strategy. Screening of effective SDS biodegraders led to the isolation of Ps...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Rebello S,Asok AK,Joseph SV,Joseph BV,Jose L,Mundayoor S,M S J

    更新日期:2013-01-01 00:00:00

  • Antioxidative Metabolites Synthesized by Marine Pigmented Vibrio sp. and Its Protection on Oxidative Deterioration of Membrane Lipids.

    abstract::Bacterial strain Vibrio sp. (PIGB 184) isolated from water samples of the Arabian Sea and identified through 16S rRNA demonstrated the production of pigmentary antioxidants with higher ABTS activities 90.9 ± 0.42 % in comparison with the standard commercial pigmented antioxidant, quercetin 88.8 ± 1.4 %. Antioxidative ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Pawar R,Mohandass C,Dastager SG,Kolekar YM,Malwankar R

    更新日期:2016-04-01 00:00:00

  • Screening of variables in beta-xylosidase recovery using cetyl trimethyl ammonium bromide reversed micelles.

    abstract::Beta-xylosidase recovery by micelles using cetyl trimethyl ammonium bromide (CTAB) cationic surfactant was verified under different experimental conditions. A 2(5-1) fractional factorial design with center points was employed to verify the influence of the following factors on enzyme extraction: pH (x1), CTAB concentr...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Hasmann FA,Pessoa A Jr,Roberto IC

    更新日期:2001-04-01 00:00:00

  • Biosynthesis of glucosyl glycerol, a compatible solute, using intermolecular transglycosylation activity of amylosucrase from Methylobacillus flagellatus KT.

    abstract::A putative α-amylase gene (accession number, CP000284) of Methylobacillus flagellatus KT ATCC51484 was cloned in Escherichia coli, and its gene product was expressed and characterized. The purified recombinant enzyme (MFAS) displayed a typical amylosucrase (ASase) activity by the demonstration of multiple activities o...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Jeong JW,Seo DH,Jung JH,Park JH,Baek NI,Kim MJ,Park CS

    更新日期:2014-06-01 00:00:00

  • Design and evaluation of an optimal controller for simultaneous saccharification and fermentation process.

    abstract::Ethanol from corn is produced using dry grind corn process in which simultaneous saccharification and fermentation (SSF) is one of the most critical unit operations. In this work an optimal controller based on a previously validated SSF model was developed by formulating the SSF process as a Bolza problem and using gr...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Murthy GS,Johnston DB,Rausch KD,Tumbleson ME,Singh V

    更新日期:2012-01-01 00:00:00

  • A simple substrate feeding strategy using a pH control trigger in fed-batch fermentation.

    abstract::A simple automated glucose feeding strategy based on pH control was developed to produce high-cell-density fed-batch fermentation. In this strategy, the pH control scheme utilized an acidified concentrated glucose solution to lower the pH. The frequency of glucose addition to the fermentor is determined by the culture...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Ting TE,Thoma GJ,Beitle RR Jr,Davis RK,Perkins R,Karim K,Liu HM

    更新日期:2008-04-01 00:00:00

  • Antibacterial activity of Alpinia galanga (L) Willd crude extracts.

    abstract::Methanol, acetone and diethyl ether extracts of Alpinia galanga have been evaluated against pathogens viz. Bacillus subtilis MTCC 2391, Enterobacter aerogene, Enterobacter cloacae, Enterococcus faecalis, Escherichia coli MTCC 1563, Klebsiella pneumoniae, Pseudomonas aeruginosa MTCC 6642, Salmonella typhimurium, Staphy...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Rao K,Ch B,Narasu LM,Giri A

    更新日期:2010-10-01 00:00:00

  • Distribution of hydrocarbon-degrading bacteria in the soil environment and their contribution to bioremediation.

    abstract::A real-time PCR quantification method for indigenous hydrocarbon-degrading bacteria (HDB) carrying the alkB gene in the soil environment was developed to investigate their distribution in soil. The detection limit of indigenous HDB by the method was 1 × 10(6) cells/g-soil. The indigenous HDB were widely distributed th...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Fukuhara Y,Horii S,Matsuno T,Matsumiya Y,Mukai M,Kubo M

    更新日期:2013-05-01 00:00:00