Impact of targeted specific antibiotic delivery for gut microbiota modulation on high-fructose-fed rats.


:The objective of present investigation was to study the effect of gut microbiota alteration by oral administration of targeted delivery of pH sensitive cefdinir microspheres to high-fructose-fed (HFD) rats. Rats were fed with a high-fructose diet with or without cefdinir microsphere administration for 30 days. The fecal microbiota community, oral glucose tolerance, the markers of liver injury, plasma and hepatic lipids profile, and histological evaluation were investigated. The levels of blood glucose, liver injury markers, lipid profile in plasma and liver, and fat tissue were significantly increased in high-fructose-fed rats. However, after pH-sensitive cefdinir microsphere administration, the elevation of these parameters was significantly suppressed. Cef EL significantly lowered the increased AST (p < 0.05) and ALT (p < 0.001) levels in HFD group. There is a significant lower (p < 0.01) AUCglucose level in Cef EL group than HFD group The histological changes in the liver and the small and large intestines were more profound in HFD group as compared to cefdinir-treated HFD and control groups. Feeding of cefdinir microsphere sustained lactobacilli and bifidobacteria and significantly decreased (p < 0.05) the number of Enterobacteriaceae induced by HFD. Experimental evidences demonstrated that the effectiveness of pH-specific cefdinir microsphere on reducing insulin resistance and development of metabolic changes in high-fructose-fed rats and suggested that it may be a promising therapeutic agent in treating type 2 diabetes. Intestinal-targeted antibiotic delivery needs to be further explored for its therapeutic applications.


Jena PK,Singh S,Prajapati B,Nareshkumar G,Mehta T,Seshadri S




Has Abstract


2014-04-01 00:00:00












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    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Xu P,Yano T,Yamamoto K,Suzuki H,Kumagai H

    更新日期:1996-03-01 00:00:00

  • Principles for efficient utilization of light for mass production of photoautotrophic microorganisms.

    abstract::Outdoor production of microalgae could be set on a sound industrial basis if solar energy were utilized at a much higher efficiency than presently obtained. Many types of photobioreactors have been developed in the past in an attempt to answer this challenge, but their photosynthetic efficiency has been rather similar...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Richmond A,Qiang H

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  • Mixed enzymic reaction--internal diffusion kinetics of nonuniformly distributed immobilized enzymes. The system agarose-micrococcal endonuclease.

    abstract::Two types of (CNBr-activated) Agarose-staphylococcal endonuclease derivatives have been prepared, one with the enzyme uniformly distributed in the support, and the other with the enzyme preferentially bound in the most external part of the support particles; the latter were obtained using agarose of very small pores a...

    journal_title:Applied biochemistry and biotechnology

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    authors: Guisan JM,Serrano J,Melo FV,Ballesteros A

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  • Isolation, purification, and characterization of two thermostable endo-1,4-β-D-glucanase forms from Opuntia vulgaris.

    abstract::Four endoglucanase temperature isoforms (T (30), T (50), T (70), and T (90)) were identified and purified from the cladodes of the xerophytic plant Opuntia vulgaris. These isoforms exhibited optimum catalytic activity at 30 °C, 50 °C, 70 °C, and 90 °C and yielded an apparent molecular mass of 150, 20, 74, and 45 kDa, ...

    journal_title:Applied biochemistry and biotechnology

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    authors: Shyamala S,Ravikumar S,Vikramathithan J,Srikumar K

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  • Evaluation of the hydrolysis specificity of protease from marine Exiguobacterium sp. SWJS2 via free amino acid analysis.

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    pub_type: 杂志文章


    authors: Lei F,Cui C,Zhao Q,Sun-Waterhouse D,Zhao M

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  • Enhanced hydrolysis and acidification of waste activated sludge by biosurfactant rhamnolipid.

    abstract::The effect of biosurfactant rhamnolipid (RL) on hydrolysis and acidification of waste activated sludge (WAS) was investigated. The results indicated that RL could greatly reduce the surface tension of sludge, resulting in stimulating the hydrolysis rate of WAS and enhancing the production of short-chain fatty acids (S...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Yi X,Luo K,Yang Q,Li XM,Deng WG,Cheng HB,Wang ZL,Zeng GM

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  • β-D-xylosidase from Geobacillus thermoleovorans IT-08: biochemical characterization and bioinformatics of the enzyme.

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    journal_title:Applied biochemistry and biotechnology

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  • Mutagenesis for Improvement of Activity and Stability of Prolyl Aminopeptidase from Aspergillus oryzae.

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    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Liu D,Zhang D,Huang Q,Gu L,Zhou N,Tian Y

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  • Inulin-containing biomass for ethanol production: carbohydrate extraction and ethanol fermentation.

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    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


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  • Potentiometric response of lipid modified ISFET.

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    authors: Osa T

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    pub_type: 杂志文章


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  • One-Step Enzymatic Synthesis of 1-Tuliposide A Using Tuliposide-Converting Enzyme.

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    pub_type: 杂志文章


    authors: Nomura T,Ogita S,Kato Y

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  • Carbonization on combustion and biodegradation of agricultural waste as a possible source of silica.

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    journal_title:Applied biochemistry and biotechnology

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    authors: Krishnamoorthy S,Iyer NR,Murthy AR

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    pub_type: 杂志文章


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  • Synergized antimicrobial activity of eugenol incorporated polyhydroxybutyrate films against food spoilage microorganisms in conjunction with pediocin.

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    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Narayanan A,Neera,Mallesha,Ramana KV

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  • An integrated bioconversion process for production of L-lactic acid from starchy potato feedstocks.

    abstract::The potential market for lactic acid as the feedstock for biodegradable polymers, oxygenated chemicals, and specialty chemicals is significant. L-lactic acid is often the desired enantiomer for such applications. However, stereospecific lactobacilli do not metabolize starch efficiently. In this work, Argonne researche...

    journal_title:Applied biochemistry and biotechnology

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    authors: Tsai SP,Moon SH

    更新日期:1998-04-01 00:00:00

  • Purification and partial characterization of bacillomycin L produced by Bacillus amyloliquefaciens K103 from lemon.

    abstract::Bacillus amyloliquefaciens K103 isolated from a lemon sample was used as a biocontrol agent to suppress Rhizoctonia solani Kühn and other fungal plant pathogens. Two antifungal compounds were purified from the culture broth using acid precipitation, gel permeation chromatography, and reversed-phase high-performance li...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


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    更新日期:2013-12-01 00:00:00

  • Effect of extruder parameters and moisture content of switchgrass, prairie cord grass on sugar recovery from enzymatic hydrolysis.

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  • RNA interference--a silent but an efficient therapeutic tool.

    abstract::RNA interference (RNAi) is an evolutionary conserved gene regulation pathway that has emerged as an important discovery in the field of molecular biology. One of the important advantages of RNAi in therapy is that it brings about efficient downregulation of gene expression by targeting complementary transcripts in com...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,评审


    authors: Ramachandran PV,Ignacimuthu S

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  • Scale up and application of biosurfactant from Bacillus subtilis in Enhanced Oil recovery.

    abstract::There is a lack of fundamental knowledge about the scale up of biosurfactant production. In order to develop suitable technology of commercialization, carrying out tests in shake flasks and bioreactors was essential. A reactor with integrated foam collector was designed for biosurfactant production using Bacillus subt...

    journal_title:Applied biochemistry and biotechnology

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    authors: Amani H,Mehrnia MR,Sarrafzadeh MH,Haghighi M,Soudi MR

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  • Methods to enhance tolerances of Chlorella KR-1 to toxic compounds in flue gas.

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    authors: Lee JN,Lee JS,Shin CS,Park SC,Kim SW

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  • Potential Application and Bactericidal Mechanism of Lactic Acid-Hydrogen Peroxide Consortium.

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    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Zhang C,Zhang S,Liu W,Guo T,Gu R,Kong J

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  • Effector-assisted refolding of recombinant tissue-plasminogen activator produced in Escherichia coli.

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    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Grunfeld H,Patel A,Shatzman A,Nishikawa AH

    更新日期:1992-05-01 00:00:00

  • Compositional changes in sugarcane bagasse on low temperature, long-term diluted ammonia treatment.

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    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Kim M,Aita G,Day DF

    更新日期:2010-05-01 00:00:00

  • Studies on productivity and characterization of polygalacturonase from Aspergillus giganteus submerged culture using citrus pectin and orange waste.

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    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Pedrolli DB,Gomes E,Monti R,Carmona EC

    更新日期:2008-02-01 00:00:00

  • L-Asparaginase as potent anti-leukemic agent and its significance of having reduced glutaminase side activity for better treatment of acute lymphoblastic leukaemia.

    abstract::Acute lymphoblastic leukaemia (ALL) is one of the leading types of malignant disorder seen in children. Viral infections, genetic factors and exposure to chemical carcinogens are some of the factors responsible for causing ALL. Treatment strategies followed for curing ALL include chemotherapy or radiation therapy, whe...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,评审


    authors: Ramya LN,Doble M,Rekha VP,Pulicherla KK

    更新日期:2012-08-01 00:00:00

  • 6-Tosyl-4,5,6,7-tetrahydrothieno[2,3-c]pyridine-3-carboxamide analogues: synthesis, characterization, MO calculation, and antibacterial activity.

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    pub_type: 杂志文章


    authors: Doshi H,Thakkar S,Khirsariya P,Thakur MC,Ray A

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    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Agibert SA,Moreira MB,Ratusznei SM,Rodrigues JA,Zaiat M,Foresti E

    更新日期:2007-02-01 00:00:00

  • Composite Electrospun Scaffold Derived from Recombinant Fibroin of Weaver Ant (Oecophylla smaragdina) as Cell-Substratum.

    abstract::Unlike silkworm (Bombyx mori) fibroin (SF), weaver ant (Oecophylla smaragdina) fibroin (WAF) is much less studied. Due to differences in amino acid composition and protein structure, this work aimed to produce the recombinant WAF protein, designated as WAF1, and investigated on its potential application as a biomateri...

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    pub_type: 杂志文章


    authors: Khamhaengpol A,Siri S

    更新日期:2017-09-01 00:00:00